Arcobacter sp. CECT 8987
Average proteome isoelectric point is 6.39
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 2486 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A4Q0XQN1|A0A4Q0XQN1_9PROT Uncharacterized protein OS=Arcobacter sp. CECT 8987 OX=2044504 GN=CRV04_06160 PE=4 SV=1
MM1 pKa = 7.67 AEE3 pKa = 4.54 SIAKK7 pKa = 8.93 VQSLLGKK14 pKa = 9.84 FYY16 pKa = 11.11 AQDD19 pKa = 2.98 EE20 pKa = 4.73 SGNVRR25 pKa = 11.84 VLKK28 pKa = 11.02 VGDD31 pKa = 3.75 TIYY34 pKa = 10.57 QGEE37 pKa = 4.57 KK38 pKa = 10.47 VYY40 pKa = 11.35 GDD42 pKa = 3.7 AANNSSDD49 pKa = 3.21 KK50 pKa = 11.09 LQIVSDD56 pKa = 3.83 TQEE59 pKa = 4.0 TFDD62 pKa = 3.63 ITGSDD67 pKa = 3.36 SLVFDD72 pKa = 4.23 TSTNEE77 pKa = 3.58 ATFGNEE83 pKa = 2.93 EE84 pKa = 4.07 VAFNPNSAWMPTADD98 pKa = 3.42 NATVPEE104 pKa = 4.79 GEE106 pKa = 4.68 TVTDD110 pKa = 3.71 AQGDD114 pKa = 3.65 ITEE117 pKa = 4.65 EE118 pKa = 3.63 EE119 pKa = 4.11 TAAGNEE125 pKa = 4.17 AASSSDD131 pKa = 2.99 VGVFNFEE138 pKa = 3.97 NRR140 pKa = 11.84 DD141 pKa = 3.35 GDD143 pKa = 4.25 SVNIQSDD150 pKa = 3.27 LRR152 pKa = 11.84 EE153 pKa = 4.19 SVFSNNSDD161 pKa = 3.28 SLVSNNGDD169 pKa = 3.43 GLNSNPSSNLLLNGTPTPDD188 pKa = 3.65 DD189 pKa = 4.12 VNDD192 pKa = 3.52 VPVAVDD198 pKa = 4.61 DD199 pKa = 5.16 SFTLDD204 pKa = 3.21 EE205 pKa = 4.74 DD206 pKa = 3.78 ASYY209 pKa = 10.33 TGTVATNDD217 pKa = 3.75 TLSGDD222 pKa = 3.79 GGNTFAVSGNPAHH235 pKa = 6.48 GTVTMNADD243 pKa = 3.29 GTFTYY248 pKa = 10.36 TPAADD253 pKa = 3.69 YY254 pKa = 11.27 NGADD258 pKa = 3.29 SFTYY262 pKa = 10.12 TITDD266 pKa = 3.42 ADD268 pKa = 4.5 GDD270 pKa = 4.11 TSTATVNLTVSSVNDD285 pKa = 3.27 VPVAVTDD292 pKa = 4.25 SINVSEE298 pKa = 4.59 EE299 pKa = 3.69 GLTNGIGDD307 pKa = 4.16 TNGTPTDD314 pKa = 3.77 TTNSNSVVGQLTIHH328 pKa = 6.31 NPDD331 pKa = 3.33 NDD333 pKa = 3.93 ALTITLNAPTDD344 pKa = 3.87 SLSSGNEE351 pKa = 3.89 TIVWSGNNTHH361 pKa = 6.2 EE362 pKa = 5.18 LIGKK366 pKa = 9.57 VGDD369 pKa = 3.7 TEE371 pKa = 4.4 VIKK374 pKa = 10.68 IAIADD379 pKa = 4.0 DD380 pKa = 3.82 GSYY383 pKa = 10.99 SVTLLDD389 pKa = 5.11 PVDD392 pKa = 4.31 HH393 pKa = 7.16 PMNDD397 pKa = 3.2 VEE399 pKa = 6.34 DD400 pKa = 3.68 ILSFNVGVNISDD412 pKa = 4.29 GEE414 pKa = 4.38 SSSSSTITVNIEE426 pKa = 3.58 DD427 pKa = 4.71 DD428 pKa = 3.9 MPTVEE433 pKa = 4.48 TSTTVWTEE441 pKa = 3.5 ATSIPDD447 pKa = 3.05 IFTGQVSFAGSSANQSRR464 pKa = 11.84 FVFANGAVLVTGKK477 pKa = 10.7 GFTSDD482 pKa = 3.35 TDD484 pKa = 3.63 LTLIDD489 pKa = 4.77 ADD491 pKa = 4.72 LNQSSGGLGVASDD504 pKa = 3.78 EE505 pKa = 4.65 SPYY508 pKa = 11.0 HH509 pKa = 5.72 NLANEE514 pKa = 3.64 VDD516 pKa = 4.14 FRR518 pKa = 11.84 KK519 pKa = 7.99 TADD522 pKa = 3.5 GEE524 pKa = 4.34 EE525 pKa = 4.52 GSEE528 pKa = 3.84 EE529 pKa = 4.91 LIIKK533 pKa = 9.88 LADD536 pKa = 3.48 GKK538 pKa = 10.46 ISYY541 pKa = 9.65 GATITFDD548 pKa = 3.4 YY549 pKa = 10.37 MFGGEE554 pKa = 4.28 EE555 pKa = 4.09 EE556 pKa = 4.12 VGEE559 pKa = 4.03 ALFYY563 pKa = 10.72 RR564 pKa = 11.84 DD565 pKa = 3.57 GVLISTQRR573 pKa = 11.84 FTSDD577 pKa = 3.2 AQSGDD582 pKa = 3.33 YY583 pKa = 10.86 AKK585 pKa = 10.82 NFEE588 pKa = 4.34 VTDD591 pKa = 3.84 GGFDD595 pKa = 3.8 TIVIRR600 pKa = 11.84 ALDD603 pKa = 3.95 NGNGYY608 pKa = 10.01 HH609 pKa = 7.43 DD610 pKa = 4.98 GDD612 pKa = 3.64 NSDD615 pKa = 4.09 FVVSGVEE622 pKa = 4.04 FLGSSQEE629 pKa = 4.04 NPISYY634 pKa = 11.0 AEE636 pKa = 3.97 GTIDD640 pKa = 4.3 YY641 pKa = 10.38 EE642 pKa = 4.65 FGADD646 pKa = 3.45 GAGSIVLTSLQNSVRR661 pKa = 11.84 LADD664 pKa = 3.6 GTEE667 pKa = 3.92 PEE669 pKa = 4.38 ITISDD674 pKa = 3.57 NTIIAKK680 pKa = 10.13 DD681 pKa = 3.52 ADD683 pKa = 3.56 GHH685 pKa = 6.73 LVFQIQFTPSTGQWEE700 pKa = 5.18 FYY702 pKa = 10.27 QYY704 pKa = 11.31 QKK706 pKa = 10.32 FTIGDD711 pKa = 3.75 GSSQTLGFNFRR722 pKa = 11.84 VTDD725 pKa = 3.81 ADD727 pKa = 3.5 GDD729 pKa = 4.32 AVNGTVSVGITNHH742 pKa = 4.92 GVEE745 pKa = 4.29 IEE747 pKa = 4.7 GIGAAQGDD755 pKa = 4.36 LTVDD759 pKa = 3.56 EE760 pKa = 5.51 ANLDD764 pKa = 4.38 DD765 pKa = 4.06 GTQPNEE771 pKa = 3.82 SDD773 pKa = 3.39 LTQSGTFTLNAMDD786 pKa = 6.08 GISEE790 pKa = 4.06 IQIGSEE796 pKa = 3.75 TFTYY800 pKa = 10.73 EE801 pKa = 3.97 EE802 pKa = 4.54 LVNAASNNISIEE814 pKa = 4.13 TDD816 pKa = 2.69 HH817 pKa = 6.48 GTLVITGYY825 pKa = 10.32 SGSEE829 pKa = 3.76 TSGTVSYY836 pKa = 10.93 SYY838 pKa = 9.46 TLKK841 pKa = 11.05 DD842 pKa = 3.48 SVDD845 pKa = 3.61 NDD847 pKa = 3.97 TQNGATSDD855 pKa = 3.93 EE856 pKa = 4.51 YY857 pKa = 11.72 VDD859 pKa = 4.01 QIDD862 pKa = 4.55 VNVTDD867 pKa = 4.59 KK868 pKa = 11.64 DD869 pKa = 3.84 GDD871 pKa = 3.9 SSSASININIQDD883 pKa = 4.34 DD884 pKa = 4.21 APVASDD890 pKa = 3.24 TTVSVDD896 pKa = 3.21 AMQEE900 pKa = 4.23 TVFNANVMLTLDD912 pKa = 3.82 YY913 pKa = 10.47 SGSMSSTALSEE924 pKa = 4.29 QKK926 pKa = 10.58 DD927 pKa = 3.65 AAKK930 pKa = 10.6 KK931 pKa = 10.78 LLDD934 pKa = 3.67 QYY936 pKa = 11.39 QKK938 pKa = 11.22 SLNEE942 pKa = 3.85 ADD944 pKa = 3.88 RR945 pKa = 11.84 GDD947 pKa = 3.81 VKK949 pKa = 11.64 VMVIKK954 pKa = 10.39 FATGAATHH962 pKa = 5.91 EE963 pKa = 4.64 KK964 pKa = 9.85 VWLTIAEE971 pKa = 4.08 AKK973 pKa = 10.47 AYY975 pKa = 9.41 IDD977 pKa = 3.04 QGKK980 pKa = 9.14 PYY982 pKa = 10.55 GIDD985 pKa = 2.9 SGTNYY990 pKa = 10.88 DD991 pKa = 4.43 DD992 pKa = 4.08 ALKK995 pKa = 9.65 TLIEE999 pKa = 4.63 NYY1001 pKa = 9.95 SSSGKK1006 pKa = 8.38 VTAEE1010 pKa = 3.59 GTINNSYY1017 pKa = 10.82 FMTDD1021 pKa = 3.47 GQPTQSNYY1029 pKa = 9.53 TSSNDD1034 pKa = 3.25 GSKK1037 pKa = 9.38 TEE1039 pKa = 4.29 DD1040 pKa = 3.41 SKK1042 pKa = 12.01 GDD1044 pKa = 3.64 GIGIGEE1050 pKa = 4.46 HH1051 pKa = 6.58 SNNPYY1056 pKa = 9.53 YY1057 pKa = 10.94 AQGTDD1062 pKa = 3.56 ANSTTYY1068 pKa = 10.81 VGTNGEE1074 pKa = 4.21 VGQGNWQAFLEE1085 pKa = 4.2 EE1086 pKa = 4.46 HH1087 pKa = 7.35 DD1088 pKa = 3.77 IVSYY1092 pKa = 10.57 AIGMGNNVQSSGLEE1106 pKa = 3.89 PIAYY1110 pKa = 9.58 DD1111 pKa = 3.59 GTSEE1115 pKa = 4.22 QQNHH1119 pKa = 6.82 DD1120 pKa = 4.48 KK1121 pKa = 10.94 LLQPQVDD1128 pKa = 4.44 FNKK1131 pKa = 10.79 LGDD1134 pKa = 4.3 LLVSTTPEE1142 pKa = 4.05 AQPVTSTLSAEE1153 pKa = 4.13 LGADD1157 pKa = 3.31 GGYY1160 pKa = 10.19 VSSITISGEE1169 pKa = 3.86 TYY1171 pKa = 10.05 TFDD1174 pKa = 3.26 GTTITNPNGTTANGHH1189 pKa = 4.99 TLCVTTEE1196 pKa = 4.13 FGTFEE1201 pKa = 4.39 INLEE1205 pKa = 4.28 TGVYY1209 pKa = 9.55 TYY1211 pKa = 10.96 TPVEE1215 pKa = 3.85 ALAQVEE1221 pKa = 4.23 QEE1223 pKa = 4.44 TIGFTVTDD1231 pKa = 3.69 NDD1233 pKa = 4.26 GDD1235 pKa = 4.04 TSSATLTIDD1244 pKa = 4.62 LSALVPDD1251 pKa = 4.21 VPTVVEE1257 pKa = 4.48 DD1258 pKa = 3.45 AHH1260 pKa = 6.44 DD1261 pKa = 3.67 TVITNEE1267 pKa = 4.11 NGASSIQAEE1276 pKa = 4.12 ASLGVVVGKK1285 pKa = 10.35 DD1286 pKa = 3.36 SAGSTVTVTNANGDD1300 pKa = 4.09 SLVGDD1305 pKa = 4.07 TVTVKK1310 pKa = 9.33 VTDD1313 pKa = 4.02 ANGSEE1318 pKa = 4.26 HH1319 pKa = 7.28 EE1320 pKa = 4.6 INATYY1325 pKa = 11.25 NDD1327 pKa = 3.48 VQLKK1331 pKa = 9.78 YY1332 pKa = 10.61 ISDD1335 pKa = 3.78 GSGGLIAVTDD1345 pKa = 4.49 DD1346 pKa = 3.06 ATQTHH1351 pKa = 6.08 VFTVEE1356 pKa = 3.64 GDD1358 pKa = 3.61 ASAGTYY1364 pKa = 9.32 KK1365 pKa = 9.56 VTMIHH1370 pKa = 6.8 ALDD1373 pKa = 3.91 PVYY1376 pKa = 10.56 SVPSILEE1383 pKa = 4.1 DD1384 pKa = 3.12 QTTQISLNSNGGSNSGINVTLNSNGGAVNQYY1415 pKa = 10.45 GNSIGINDD1423 pKa = 4.16 YY1424 pKa = 11.32 DD1425 pKa = 3.84 VGSAKK1430 pKa = 10.75 YY1431 pKa = 9.45 EE1432 pKa = 3.84 IEE1434 pKa = 4.42 GYY1436 pKa = 10.03 EE1437 pKa = 4.05 VLSMNFNALTGVSVSKK1453 pKa = 11.0 VSVNFNDD1460 pKa = 4.59 FGYY1463 pKa = 10.79 HH1464 pKa = 6.91 DD1465 pKa = 4.17 DD1466 pKa = 5.23 AIVKK1470 pKa = 9.41 VYY1472 pKa = 9.47 YY1473 pKa = 10.1 TDD1475 pKa = 3.43 NTYY1478 pKa = 9.23 DD1479 pKa = 3.19 TYY1481 pKa = 11.27 EE1482 pKa = 3.72 VHH1484 pKa = 7.28 GDD1486 pKa = 3.36 YY1487 pKa = 10.88 NDD1489 pKa = 3.89 RR1490 pKa = 11.84 DD1491 pKa = 4.26 GIDD1494 pKa = 3.08 IEE1496 pKa = 4.24 IPEE1499 pKa = 4.79 GKK1501 pKa = 7.78 TLSRR1505 pKa = 11.84 IDD1507 pKa = 4.52 FGAEE1511 pKa = 3.79 DD1512 pKa = 4.7 YY1513 pKa = 11.65 YY1514 pKa = 11.59 DD1515 pKa = 4.55 DD1516 pKa = 4.54 YY1517 pKa = 11.83 SVNSTITVVYY1527 pKa = 9.62 DD1528 pKa = 3.45 KK1529 pKa = 11.22 QSTIPVDD1536 pKa = 3.61 NPEE1539 pKa = 3.84 QTLEE1543 pKa = 4.19 FGAVVTDD1550 pKa = 3.93 GNGDD1554 pKa = 3.24 QSEE1557 pKa = 4.69 VVTFDD1562 pKa = 3.12 VTVDD1566 pKa = 3.5 SDD1568 pKa = 3.9 HH1569 pKa = 6.65 VLQGDD1574 pKa = 3.75 VSDD1577 pKa = 5.17 SIIGGDD1583 pKa = 3.77 GDD1585 pKa = 4.63 DD1586 pKa = 3.99 TFKK1589 pKa = 11.37 LLNGQDD1595 pKa = 3.36 INFDD1599 pKa = 3.61 GLDD1602 pKa = 3.35 AHH1604 pKa = 7.3 IKK1606 pKa = 10.21 NIEE1609 pKa = 4.23 TIDD1612 pKa = 3.77 LSAEE1616 pKa = 3.97 GKK1618 pKa = 10.23 NEE1620 pKa = 3.74 IKK1622 pKa = 10.62 NLSLDD1627 pKa = 3.92 DD1628 pKa = 4.76 VIDD1631 pKa = 3.66 MTDD1634 pKa = 3.0 SDD1636 pKa = 4.55 NEE1638 pKa = 4.05 IKK1640 pKa = 10.48 ISGTSEE1646 pKa = 4.66 DD1647 pKa = 4.24 EE1648 pKa = 4.11 VHH1650 pKa = 6.52 LTNEE1654 pKa = 3.44 WSTNNVVDD1662 pKa = 3.59 ADD1664 pKa = 4.4 GYY1666 pKa = 10.49 IEE1668 pKa = 4.8 YY1669 pKa = 10.06 IGTSDD1674 pKa = 4.71 DD1675 pKa = 3.36 EE1676 pKa = 4.59 TVKK1679 pKa = 11.01 VKK1681 pKa = 10.1 IHH1683 pKa = 5.93 QDD1685 pKa = 2.39 IHH1687 pKa = 7.2 TDD1689 pKa = 3.08 IQQ1691 pKa = 3.44
Molecular weight: 178.88 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.737
IPC2_protein 3.605
IPC_protein 3.668
Toseland 3.427
ProMoST 3.821
Dawson 3.656
Bjellqvist 3.808
Wikipedia 3.592
Rodwell 3.478
Grimsley 3.338
Solomon 3.656
Lehninger 3.617
Nozaki 3.757
DTASelect 4.037
Thurlkill 3.478
EMBOSS 3.605
Sillero 3.783
Patrickios 1.837
IPC_peptide 3.656
IPC2_peptide 3.757
IPC2.peptide.svr19 3.72
Protein with the highest isoelectric point:
>tr|A0A4Q0XQW7|A0A4Q0XQW7_9PROT Bcr/CflA family efflux transporter OS=Arcobacter sp. CECT 8987 OX=2044504 GN=CRV04_06635 PE=3 SV=1
MM1 pKa = 7.28 KK2 pKa = 9.44 RR3 pKa = 11.84 TYY5 pKa = 10.04 QPHH8 pKa = 4.86 NTPRR12 pKa = 11.84 KK13 pKa = 7.31 RR14 pKa = 11.84 THH16 pKa = 5.88 GFRR19 pKa = 11.84 VRR21 pKa = 11.84 MKK23 pKa = 8.73 TKK25 pKa = 10.05 SGRR28 pKa = 11.84 KK29 pKa = 8.71 VISARR34 pKa = 11.84 RR35 pKa = 11.84 AKK37 pKa = 9.87 GRR39 pKa = 11.84 KK40 pKa = 8.75 RR41 pKa = 11.84 LAVV44 pKa = 3.41
Molecular weight: 5.25 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.414
IPC2_protein 11.096
IPC_protein 12.457
Toseland 12.618
ProMoST 13.1
Dawson 12.618
Bjellqvist 12.603
Wikipedia 13.086
Rodwell 12.384
Grimsley 12.661
Solomon 13.1
Lehninger 13.013
Nozaki 12.618
DTASelect 12.603
Thurlkill 12.618
EMBOSS 13.115
Sillero 12.618
Patrickios 12.106
IPC_peptide 13.115
IPC2_peptide 12.091
IPC2.peptide.svr19 9.021
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
2486
0
2486
787083
37
1691
316.6
35.89
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
6.481 ± 0.062
0.962 ± 0.018
5.292 ± 0.04
7.266 ± 0.047
5.161 ± 0.044
5.513 ± 0.06
2.049 ± 0.024
8.576 ± 0.052
8.449 ± 0.054
9.825 ± 0.06
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.568 ± 0.024
5.663 ± 0.039
2.896 ± 0.029
3.695 ± 0.033
3.033 ± 0.028
6.28 ± 0.033
5.298 ± 0.042
6.268 ± 0.035
0.724 ± 0.017
4.002 ± 0.032
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here