Mesocricetibacter intestinalis
Average proteome isoelectric point is 6.74
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 1827 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A4R6V894|A0A4R6V894_9PAST 3-oxoacyl-[acyl-carrier-protein] synthase-1 OS=Mesocricetibacter intestinalis OX=1521930 GN=EDC45_1319 PE=3 SV=1
MM1 pKa = 7.56 TDD3 pKa = 2.63 IAVPLTFTDD12 pKa = 3.63 AAANKK17 pKa = 8.89 VKK19 pKa = 10.76 ALISDD24 pKa = 4.41 EE25 pKa = 4.31 GNQDD29 pKa = 2.99 LKK31 pKa = 11.28 LRR33 pKa = 11.84 VYY35 pKa = 9.2 ITGGGCSGFQYY46 pKa = 11.02 GFTFDD51 pKa = 3.71 EE52 pKa = 4.79 KK53 pKa = 11.47 VNEE56 pKa = 4.16 GDD58 pKa = 3.82 LTVEE62 pKa = 3.97 NAGVQLVIDD71 pKa = 4.36 PMSLQYY77 pKa = 11.06 LIGGTVDD84 pKa = 3.33 YY85 pKa = 11.19 TEE87 pKa = 4.43 GLEE90 pKa = 4.07 GSRR93 pKa = 11.84 FIVNNPNATTTCGCGSSFSII113 pKa = 5.02
Molecular weight: 11.97 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.814
IPC2_protein 4.062
IPC_protein 3.973
Toseland 3.77
ProMoST 4.139
Dawson 3.961
Bjellqvist 4.113
Wikipedia 3.897
Rodwell 3.808
Grimsley 3.694
Solomon 3.948
Lehninger 3.897
Nozaki 4.088
DTASelect 4.279
Thurlkill 3.834
EMBOSS 3.897
Sillero 4.088
Patrickios 1.939
IPC_peptide 3.935
IPC2_peptide 4.075
IPC2.peptide.svr19 3.994
Protein with the highest isoelectric point:
>tr|A0A4R6V6I1|A0A4R6V6I1_9PAST Molybdopterin-guanine dinucleotide biosynthesis protein B OS=Mesocricetibacter intestinalis OX=1521930 GN=EDC45_1944 PE=4 SV=1
MM1 pKa = 7.45 KK2 pKa = 9.51 RR3 pKa = 11.84 TFQPSVLKK11 pKa = 10.6 RR12 pKa = 11.84 SRR14 pKa = 11.84 THH16 pKa = 6.4 GFRR19 pKa = 11.84 ARR21 pKa = 11.84 MATKK25 pKa = 10.4 NGRR28 pKa = 11.84 QVLARR33 pKa = 11.84 RR34 pKa = 11.84 RR35 pKa = 11.84 AKK37 pKa = 10.17 GRR39 pKa = 11.84 KK40 pKa = 8.79 KK41 pKa = 10.63 LSAA44 pKa = 3.95
Molecular weight: 5.14 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.517
IPC2_protein 11.213
IPC_protein 12.793
Toseland 12.969
ProMoST 13.451
Dawson 12.969
Bjellqvist 12.954
Wikipedia 13.437
Rodwell 12.676
Grimsley 12.998
Solomon 13.451
Lehninger 13.364
Nozaki 12.969
DTASelect 12.954
Thurlkill 12.969
EMBOSS 13.466
Sillero 12.969
Patrickios 12.398
IPC_peptide 13.466
IPC2_peptide 12.442
IPC2.peptide.svr19 9.148
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
1827
0
1827
598647
30
3475
327.7
36.4
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
9.461 ± 0.107
1.139 ± 0.027
4.831 ± 0.047
6.142 ± 0.061
4.237 ± 0.054
7.192 ± 0.088
2.044 ± 0.031
6.833 ± 0.055
5.454 ± 0.044
10.952 ± 0.109
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.398 ± 0.03
4.352 ± 0.073
3.951 ± 0.038
4.709 ± 0.053
5.167 ± 0.063
5.919 ± 0.063
4.666 ± 0.065
6.264 ± 0.059
1.125 ± 0.024
3.164 ± 0.036
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here