Bacillus phage Staley

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Slashvirus; Bacillus virus Staley

Average proteome isoelectric point is 6.39

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 113 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|U5Q1F5|U5Q1F5_9CAUD Uncharacterized protein OS=Bacillus phage Staley OX=1406792 GN=Staley_75 PE=4 SV=1
MM1 pKa = 6.21QTRR4 pKa = 11.84NEE6 pKa = 4.81LITMEE11 pKa = 4.09EE12 pKa = 4.2SKK14 pKa = 10.91LEE16 pKa = 4.1SMFRR20 pKa = 11.84KK21 pKa = 9.35EE22 pKa = 4.04LSNFEE27 pKa = 3.94IMGIEE32 pKa = 3.89AFLDD36 pKa = 3.06EE37 pKa = 5.19WYY39 pKa = 10.5RR40 pKa = 11.84EE41 pKa = 4.02YY42 pKa = 11.44YY43 pKa = 10.82GFGEE47 pKa = 4.24EE48 pKa = 4.63EE49 pKa = 4.05KK50 pKa = 10.35ATEE53 pKa = 4.1NFLSLAYY60 pKa = 9.29TSSSSYY66 pKa = 11.8AMINEE71 pKa = 4.37CGNAEE76 pKa = 4.16FNEE79 pKa = 4.24LTLSHH84 pKa = 7.36FALTEE89 pKa = 3.94NNLLVAVCHH98 pKa = 6.84DD99 pKa = 3.16IEE101 pKa = 4.44EE102 pKa = 4.5NYY104 pKa = 10.87SYY106 pKa = 11.53FWMNN110 pKa = 2.94

Molecular weight: 13.0 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|U5Q1B1|U5Q1B1_9CAUD Uncharacterized protein OS=Bacillus phage Staley OX=1406792 GN=Staley_20 PE=4 SV=1
MM1 pKa = 7.54KK2 pKa = 10.29SKK4 pKa = 10.83KK5 pKa = 10.26EE6 pKa = 3.9YY7 pKa = 10.71GQDD10 pKa = 2.94FLTRR14 pKa = 11.84PRR16 pKa = 11.84KK17 pKa = 9.39MSTDD21 pKa = 3.33QEE23 pKa = 4.32KK24 pKa = 10.27VANSVFLWRR33 pKa = 11.84FDD35 pKa = 3.45KK36 pKa = 11.07EE37 pKa = 4.18NAVFTLSYY45 pKa = 10.61LGVANGILNRR55 pKa = 11.84INLMLAAEE63 pKa = 4.36VEE65 pKa = 4.11NGFINKK71 pKa = 9.29YY72 pKa = 6.37MLRR75 pKa = 11.84KK76 pKa = 9.49RR77 pKa = 11.84VRR79 pKa = 3.1

Molecular weight: 9.29 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

113

0

113

24423

25

2249

216.1

24.58

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.232 ± 0.406

0.897 ± 0.134

6.269 ± 0.156

7.477 ± 0.414

4.647 ± 0.216

6.289 ± 0.255

1.646 ± 0.142

6.51 ± 0.178

8.848 ± 0.317

7.702 ± 0.222

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

3.014 ± 0.194

5.953 ± 0.235

2.682 ± 0.176

4.111 ± 0.278

4.017 ± 0.206

5.826 ± 0.26

6.26 ± 0.348

6.539 ± 0.164

1.114 ± 0.079

3.968 ± 0.153
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski