Amino acid dipepetide frequency for Bacillus phage Staley

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
4.709AlaAla: 4.709 ± 0.7
0.491AlaCys: 0.491 ± 0.155
3.849AlaAsp: 3.849 ± 0.603
4.586AlaGlu: 4.586 ± 0.492
2.621AlaPhe: 2.621 ± 0.449
4.177AlaGly: 4.177 ± 0.604
0.983AlaHis: 0.983 ± 0.186
4.34AlaIle: 4.34 ± 0.497
5.487AlaLys: 5.487 ± 0.691
3.849AlaLeu: 3.849 ± 0.364
1.761AlaMet: 1.761 ± 0.433
4.177AlaAsn: 4.177 ± 0.467
1.228AlaPro: 1.228 ± 0.261
2.662AlaGln: 2.662 ± 0.483
2.006AlaArg: 2.006 ± 0.293
3.726AlaSer: 3.726 ± 0.548
3.931AlaThr: 3.931 ± 0.622
4.381AlaVal: 4.381 ± 0.426
0.737AlaTrp: 0.737 ± 0.193
2.621AlaTyr: 2.621 ± 0.328
0.0AlaXaa: 0.0 ± 0.0
Cys
0.328CysAla: 0.328 ± 0.116
0.041CysCys: 0.041 ± 0.046
0.696CysAsp: 0.696 ± 0.196
0.655CysGlu: 0.655 ± 0.169
0.328CysPhe: 0.328 ± 0.131
0.819CysGly: 0.819 ± 0.235
0.287CysHis: 0.287 ± 0.126
0.491CysIle: 0.491 ± 0.135
0.655CysLys: 0.655 ± 0.204
0.614CysLeu: 0.614 ± 0.197
0.123CysMet: 0.123 ± 0.087
0.778CysAsn: 0.778 ± 0.214
0.409CysPro: 0.409 ± 0.154
0.287CysGln: 0.287 ± 0.109
0.328CysArg: 0.328 ± 0.127
0.532CysSer: 0.532 ± 0.173
0.573CysThr: 0.573 ± 0.174
0.614CysVal: 0.614 ± 0.18
0.123CysTrp: 0.123 ± 0.076
0.287CysTyr: 0.287 ± 0.132
0.0CysXaa: 0.0 ± 0.0
Asp
2.948AspAla: 2.948 ± 0.287
0.614AspCys: 0.614 ± 0.151
3.685AspAsp: 3.685 ± 0.385
4.545AspGlu: 4.545 ± 0.553
3.071AspPhe: 3.071 ± 0.308
4.75AspGly: 4.75 ± 0.558
0.778AspHis: 0.778 ± 0.202
4.709AspIle: 4.709 ± 0.422
4.995AspLys: 4.995 ± 0.396
5.282AspLeu: 5.282 ± 0.509
1.965AspMet: 1.965 ± 0.237
2.989AspAsn: 2.989 ± 0.369
1.761AspPro: 1.761 ± 0.262
2.539AspGln: 2.539 ± 0.363
2.293AspArg: 2.293 ± 0.354
3.644AspSer: 3.644 ± 0.498
3.603AspThr: 3.603 ± 0.386
5.077AspVal: 5.077 ± 0.566
0.655AspTrp: 0.655 ± 0.158
2.784AspTyr: 2.784 ± 0.48
0.0AspXaa: 0.0 ± 0.0
Glu
3.808GluAla: 3.808 ± 0.532
1.024GluCys: 1.024 ± 0.237
3.726GluAsp: 3.726 ± 0.443
7.125GluGlu: 7.125 ± 0.804
3.48GluPhe: 3.48 ± 0.423
4.381GluGly: 4.381 ± 0.469
1.269GluHis: 1.269 ± 0.23
4.136GluIle: 4.136 ± 0.516
6.47GluLys: 6.47 ± 0.686
6.756GluLeu: 6.756 ± 0.587
3.03GluMet: 3.03 ± 0.395
4.586GluAsn: 4.586 ± 0.468
1.269GluPro: 1.269 ± 0.303
3.071GluGln: 3.071 ± 0.359
3.194GluArg: 3.194 ± 0.51
3.767GluSer: 3.767 ± 0.343
4.422GluThr: 4.422 ± 0.433
5.118GluVal: 5.118 ± 0.62
1.269GluTrp: 1.269 ± 0.257
2.866GluTyr: 2.866 ± 0.342
0.0GluXaa: 0.0 ± 0.0
Phe
2.784PheAla: 2.784 ± 0.287
0.45PheCys: 0.45 ± 0.138
3.562PheAsp: 3.562 ± 0.303
3.849PheGlu: 3.849 ± 0.407
1.761PhePhe: 1.761 ± 0.303
3.03PheGly: 3.03 ± 0.439
0.778PheHis: 0.778 ± 0.212
2.825PheIle: 2.825 ± 0.404
3.644PheLys: 3.644 ± 0.321
3.89PheLeu: 3.89 ± 0.581
0.983PheMet: 0.983 ± 0.215
3.276PheAsn: 3.276 ± 0.347
1.638PhePro: 1.638 ± 0.301
1.515PheGln: 1.515 ± 0.254
1.843PheArg: 1.843 ± 0.301
2.743PheSer: 2.743 ± 0.35
3.48PheThr: 3.48 ± 0.392
2.539PheVal: 2.539 ± 0.326
0.328PheTrp: 0.328 ± 0.125
1.556PheTyr: 1.556 ± 0.294
0.0PheXaa: 0.0 ± 0.0
Gly
3.48GlyAla: 3.48 ± 0.447
0.328GlyCys: 0.328 ± 0.115
3.562GlyAsp: 3.562 ± 0.392
4.095GlyGlu: 4.095 ± 0.398
3.03GlyPhe: 3.03 ± 0.487
4.34GlyGly: 4.34 ± 0.574
0.942GlyHis: 0.942 ± 0.166
4.709GlyIle: 4.709 ± 0.477
5.569GlyLys: 5.569 ± 0.572
5.446GlyLeu: 5.446 ± 0.472
1.802GlyMet: 1.802 ± 0.291
3.276GlyAsn: 3.276 ± 0.457
0.369GlyPro: 0.369 ± 0.125
3.071GlyGln: 3.071 ± 0.49
2.129GlyArg: 2.129 ± 0.288
4.463GlySer: 4.463 ± 0.484
3.644GlyThr: 3.644 ± 0.522
4.545GlyVal: 4.545 ± 0.46
0.778GlyTrp: 0.778 ± 0.157
3.317GlyTyr: 3.317 ± 0.409
0.0GlyXaa: 0.0 ± 0.0
His
0.819HisAla: 0.819 ± 0.184
0.123HisCys: 0.123 ± 0.072
1.31HisAsp: 1.31 ± 0.29
1.474HisGlu: 1.474 ± 0.312
1.187HisPhe: 1.187 ± 0.274
0.737HisGly: 0.737 ± 0.188
0.532HisHis: 0.532 ± 0.187
1.147HisIle: 1.147 ± 0.242
1.638HisLys: 1.638 ± 0.295
1.515HisLeu: 1.515 ± 0.366
0.205HisMet: 0.205 ± 0.1
0.491HisAsn: 0.491 ± 0.146
0.491HisPro: 0.491 ± 0.152
0.655HisGln: 0.655 ± 0.164
0.655HisArg: 0.655 ± 0.173
0.573HisSer: 0.573 ± 0.125
1.065HisThr: 1.065 ± 0.217
0.901HisVal: 0.901 ± 0.205
0.246HisTrp: 0.246 ± 0.128
0.696HisTyr: 0.696 ± 0.145
0.0HisXaa: 0.0 ± 0.0
Ile
4.218IleAla: 4.218 ± 0.458
0.655IleCys: 0.655 ± 0.18
5.036IleAsp: 5.036 ± 0.472
5.036IleGlu: 5.036 ± 0.561
2.702IlePhe: 2.702 ± 0.323
4.177IleGly: 4.177 ± 0.438
1.187IleHis: 1.187 ± 0.271
3.89IleIle: 3.89 ± 0.423
6.306IleLys: 6.306 ± 0.465
3.972IleLeu: 3.972 ± 0.423
1.884IleMet: 1.884 ± 0.367
4.013IleAsn: 4.013 ± 0.389
1.884IlePro: 1.884 ± 0.224
2.334IleGln: 2.334 ± 0.293
2.662IleArg: 2.662 ± 0.371
3.767IleSer: 3.767 ± 0.407
4.504IleThr: 4.504 ± 0.318
4.258IleVal: 4.258 ± 0.542
0.573IleTrp: 0.573 ± 0.188
2.047IleTyr: 2.047 ± 0.25
0.0IleXaa: 0.0 ± 0.0
Lys
6.265LysAla: 6.265 ± 0.777
0.696LysCys: 0.696 ± 0.214
5.282LysAsp: 5.282 ± 0.554
7.043LysGlu: 7.043 ± 0.648
3.726LysPhe: 3.726 ± 0.333
5.159LysGly: 5.159 ± 0.412
1.269LysHis: 1.269 ± 0.206
5.118LysIle: 5.118 ± 0.604
7.985LysLys: 7.985 ± 0.691
6.879LysLeu: 6.879 ± 0.635
2.621LysMet: 2.621 ± 0.34
5.651LysAsn: 5.651 ± 0.458
2.743LysPro: 2.743 ± 0.444
3.194LysGln: 3.194 ± 0.445
4.463LysArg: 4.463 ± 0.504
4.422LysSer: 4.422 ± 0.437
5.364LysThr: 5.364 ± 0.497
6.06LysVal: 6.06 ± 0.552
0.983LysTrp: 0.983 ± 0.205
3.562LysTyr: 3.562 ± 0.368
0.0LysXaa: 0.0 ± 0.0
Leu
5.528LeuAla: 5.528 ± 0.618
0.737LeuCys: 0.737 ± 0.194
5.773LeuAsp: 5.773 ± 0.535
6.674LeuGlu: 6.674 ± 0.791
2.662LeuPhe: 2.662 ± 0.337
4.095LeuGly: 4.095 ± 0.416
1.187LeuHis: 1.187 ± 0.179
4.299LeuIle: 4.299 ± 0.506
7.084LeuLys: 7.084 ± 0.656
5.405LeuLeu: 5.405 ± 0.471
2.17LeuMet: 2.17 ± 0.382
4.832LeuAsn: 4.832 ± 0.46
2.17LeuPro: 2.17 ± 0.339
2.989LeuGln: 2.989 ± 0.367
2.948LeuArg: 2.948 ± 0.364
5.282LeuSer: 5.282 ± 0.478
4.75LeuThr: 4.75 ± 0.418
4.545LeuVal: 4.545 ± 0.442
1.024LeuTrp: 1.024 ± 0.267
2.866LeuTyr: 2.866 ± 0.33
0.0LeuXaa: 0.0 ± 0.0
Met
1.638MetAla: 1.638 ± 0.285
0.164MetCys: 0.164 ± 0.094
1.884MetAsp: 1.884 ± 0.267
2.375MetGlu: 2.375 ± 0.379
1.515MetPhe: 1.515 ± 0.256
1.72MetGly: 1.72 ± 0.501
0.409MetHis: 0.409 ± 0.133
2.58MetIle: 2.58 ± 0.373
2.784MetLys: 2.784 ± 0.483
2.006MetLeu: 2.006 ± 0.307
0.942MetMet: 0.942 ± 0.218
2.211MetAsn: 2.211 ± 0.337
1.065MetPro: 1.065 ± 0.244
1.269MetGln: 1.269 ± 0.257
1.024MetArg: 1.024 ± 0.212
2.211MetSer: 2.211 ± 0.279
1.965MetThr: 1.965 ± 0.317
1.392MetVal: 1.392 ± 0.268
0.328MetTrp: 0.328 ± 0.119
0.655MetTyr: 0.655 ± 0.219
0.0MetXaa: 0.0 ± 0.0
Asn
3.603AsnAla: 3.603 ± 0.546
0.45AsnCys: 0.45 ± 0.153
3.931AsnAsp: 3.931 ± 0.333
4.299AsnGlu: 4.299 ± 0.437
2.58AsnPhe: 2.58 ± 0.381
4.75AsnGly: 4.75 ± 0.481
0.942AsnHis: 0.942 ± 0.244
2.866AsnIle: 2.866 ± 0.349
5.61AsnLys: 5.61 ± 0.518
4.668AsnLeu: 4.668 ± 0.461
1.72AsnMet: 1.72 ± 0.314
3.521AsnAsn: 3.521 ± 0.415
2.702AsnPro: 2.702 ± 0.353
2.211AsnGln: 2.211 ± 0.341
2.784AsnArg: 2.784 ± 0.355
2.866AsnSer: 2.866 ± 0.406
3.931AsnThr: 3.931 ± 0.49
3.399AsnVal: 3.399 ± 0.459
0.614AsnTrp: 0.614 ± 0.179
2.088AsnTyr: 2.088 ± 0.303
0.0AsnXaa: 0.0 ± 0.0
Pro
1.761ProAla: 1.761 ± 0.282
0.164ProCys: 0.164 ± 0.09
1.269ProAsp: 1.269 ± 0.312
1.884ProGlu: 1.884 ± 0.314
1.474ProPhe: 1.474 ± 0.237
1.228ProGly: 1.228 ± 0.214
0.655ProHis: 0.655 ± 0.154
2.088ProIle: 2.088 ± 0.364
2.743ProLys: 2.743 ± 0.437
1.761ProLeu: 1.761 ± 0.284
0.778ProMet: 0.778 ± 0.215
1.679ProAsn: 1.679 ± 0.308
0.491ProPro: 0.491 ± 0.175
0.983ProGln: 0.983 ± 0.199
0.86ProArg: 0.86 ± 0.179
2.211ProSer: 2.211 ± 0.326
1.965ProThr: 1.965 ± 0.32
1.392ProVal: 1.392 ± 0.303
0.123ProTrp: 0.123 ± 0.068
1.31ProTyr: 1.31 ± 0.209
0.0ProXaa: 0.0 ± 0.0
Gln
3.358GlnAla: 3.358 ± 0.657
0.246GlnCys: 0.246 ± 0.114
1.843GlnAsp: 1.843 ± 0.275
2.252GlnGlu: 2.252 ± 0.436
1.924GlnPhe: 1.924 ± 0.344
1.924GlnGly: 1.924 ± 0.303
0.573GlnHis: 0.573 ± 0.127
2.702GlnIle: 2.702 ± 0.393
3.358GlnLys: 3.358 ± 0.38
4.136GlnLeu: 4.136 ± 0.519
1.597GlnMet: 1.597 ± 0.387
2.334GlnAsn: 2.334 ± 0.388
1.065GlnPro: 1.065 ± 0.204
3.153GlnGln: 3.153 ± 0.859
1.884GlnArg: 1.884 ± 0.303
2.375GlnSer: 2.375 ± 0.399
1.965GlnThr: 1.965 ± 0.31
2.948GlnVal: 2.948 ± 0.429
0.328GlnTrp: 0.328 ± 0.102
1.147GlnTyr: 1.147 ± 0.191
0.0GlnXaa: 0.0 ± 0.0
Arg
1.638ArgAla: 1.638 ± 0.321
0.328ArgCys: 0.328 ± 0.137
2.457ArgAsp: 2.457 ± 0.29
2.621ArgGlu: 2.621 ± 0.364
2.498ArgPhe: 2.498 ± 0.439
2.375ArgGly: 2.375 ± 0.39
0.532ArgHis: 0.532 ± 0.132
3.562ArgIle: 3.562 ± 0.467
4.75ArgLys: 4.75 ± 0.628
2.457ArgLeu: 2.457 ± 0.404
1.556ArgMet: 1.556 ± 0.275
2.047ArgAsn: 2.047 ± 0.309
1.187ArgPro: 1.187 ± 0.277
1.351ArgGln: 1.351 ± 0.26
1.679ArgArg: 1.679 ± 0.348
1.72ArgSer: 1.72 ± 0.327
2.743ArgThr: 2.743 ± 0.368
2.825ArgVal: 2.825 ± 0.354
0.614ArgTrp: 0.614 ± 0.177
1.228ArgTyr: 1.228 ± 0.236
0.0ArgXaa: 0.0 ± 0.0
Ser
3.235SerAla: 3.235 ± 0.488
0.614SerCys: 0.614 ± 0.199
3.153SerAsp: 3.153 ± 0.364
4.054SerGlu: 4.054 ± 0.402
2.825SerPhe: 2.825 ± 0.381
4.422SerGly: 4.422 ± 0.603
0.901SerHis: 0.901 ± 0.215
3.603SerIle: 3.603 ± 0.409
4.34SerLys: 4.34 ± 0.505
4.832SerLeu: 4.832 ± 0.498
2.293SerMet: 2.293 ± 0.301
3.317SerAsn: 3.317 ± 0.436
1.597SerPro: 1.597 ± 0.209
2.58SerGln: 2.58 ± 0.512
1.72SerArg: 1.72 ± 0.202
3.89SerSer: 3.89 ± 0.423
3.521SerThr: 3.521 ± 0.503
3.48SerVal: 3.48 ± 0.403
0.655SerTrp: 0.655 ± 0.151
3.194SerTyr: 3.194 ± 0.409
0.0SerXaa: 0.0 ± 0.0
Thr
4.504ThrAla: 4.504 ± 0.842
0.45ThrCys: 0.45 ± 0.133
3.849ThrAsp: 3.849 ± 0.416
3.972ThrGlu: 3.972 ± 0.358
3.89ThrPhe: 3.89 ± 0.446
3.767ThrGly: 3.767 ± 0.407
0.983ThrHis: 0.983 ± 0.198
3.849ThrIle: 3.849 ± 0.497
4.75ThrLys: 4.75 ± 0.458
5.118ThrLeu: 5.118 ± 0.416
1.474ThrMet: 1.474 ± 0.294
3.685ThrAsn: 3.685 ± 0.302
2.17ThrPro: 2.17 ± 0.273
2.58ThrGln: 2.58 ± 0.404
1.965ThrArg: 1.965 ± 0.284
3.726ThrSer: 3.726 ± 0.444
4.054ThrThr: 4.054 ± 0.579
4.586ThrVal: 4.586 ± 0.371
0.696ThrTrp: 0.696 ± 0.138
2.539ThrTyr: 2.539 ± 0.3
0.0ThrXaa: 0.0 ± 0.0
Val
3.972ValAla: 3.972 ± 0.409
0.532ValCys: 0.532 ± 0.223
4.299ValAsp: 4.299 ± 0.449
4.668ValGlu: 4.668 ± 0.489
3.112ValPhe: 3.112 ± 0.377
3.89ValGly: 3.89 ± 0.52
1.065ValHis: 1.065 ± 0.225
4.955ValIle: 4.955 ± 0.527
6.06ValLys: 6.06 ± 0.518
4.258ValLeu: 4.258 ± 0.347
1.884ValMet: 1.884 ± 0.33
3.685ValAsn: 3.685 ± 0.308
1.802ValPro: 1.802 ± 0.267
3.03ValGln: 3.03 ± 0.347
3.235ValArg: 3.235 ± 0.383
3.562ValSer: 3.562 ± 0.388
4.054ValThr: 4.054 ± 0.421
4.218ValVal: 4.218 ± 0.363
0.573ValTrp: 0.573 ± 0.134
2.539ValTyr: 2.539 ± 0.337
0.0ValXaa: 0.0 ± 0.0
Trp
0.532TrpAla: 0.532 ± 0.144
0.246TrpCys: 0.246 ± 0.127
0.655TrpAsp: 0.655 ± 0.15
0.737TrpGlu: 0.737 ± 0.221
0.45TrpPhe: 0.45 ± 0.17
0.819TrpGly: 0.819 ± 0.166
0.409TrpHis: 0.409 ± 0.146
0.901TrpIle: 0.901 ± 0.196
0.901TrpLys: 0.901 ± 0.182
0.901TrpLeu: 0.901 ± 0.182
0.328TrpMet: 0.328 ± 0.107
0.369TrpAsn: 0.369 ± 0.131
0.123TrpPro: 0.123 ± 0.057
0.369TrpGln: 0.369 ± 0.165
0.655TrpArg: 0.655 ± 0.193
0.655TrpSer: 0.655 ± 0.142
0.778TrpThr: 0.778 ± 0.188
0.532TrpVal: 0.532 ± 0.142
0.082TrpTrp: 0.082 ± 0.063
0.696TrpTyr: 0.696 ± 0.195
0.0TrpXaa: 0.0 ± 0.0
Tyr
3.194TyrAla: 3.194 ± 0.406
0.614TyrCys: 0.614 ± 0.178
2.866TyrAsp: 2.866 ± 0.342
2.825TyrGlu: 2.825 ± 0.421
1.638TyrPhe: 1.638 ± 0.267
2.334TyrGly: 2.334 ± 0.277
0.778TyrHis: 0.778 ± 0.177
2.334TyrIle: 2.334 ± 0.384
3.358TyrLys: 3.358 ± 0.327
3.276TyrLeu: 3.276 ± 0.39
0.983TyrMet: 0.983 ± 0.226
2.621TyrAsn: 2.621 ± 0.348
0.655TyrPro: 0.655 ± 0.192
1.269TyrGln: 1.269 ± 0.208
1.843TyrArg: 1.843 ± 0.332
2.129TyrSer: 2.129 ± 0.303
2.293TyrThr: 2.293 ± 0.284
2.58TyrVal: 2.58 ± 0.401
0.409TyrTrp: 0.409 ± 0.133
1.679TyrTyr: 1.679 ± 0.366
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 113 proteins (24423 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski