Candidatus Terasakiella magnetica
Average proteome isoelectric point is 6.19
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 3572 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A1C3RGI3|A0A1C3RGI3_9PROT Chaperone protein DnaJ OS=Candidatus Terasakiella magnetica OX=1867952 GN=dnaJ PE=3 SV=1
MM1 pKa = 7.66 IKK3 pKa = 10.62 LLLGTAALSVMSLISVGANAADD25 pKa = 4.66 PIKK28 pKa = 10.78 LSFSGFMEE36 pKa = 3.77 QWVGYY41 pKa = 9.9 SDD43 pKa = 4.88 NEE45 pKa = 4.24 GDD47 pKa = 3.89 NGAANYY53 pKa = 10.69 SEE55 pKa = 4.79 VGTFSDD61 pKa = 4.48 VEE63 pKa = 4.29 LFMKK67 pKa = 10.83 GSTRR71 pKa = 11.84 LDD73 pKa = 3.2 NGLTVGVNFEE83 pKa = 4.18 IEE85 pKa = 4.03 RR86 pKa = 11.84 SGGADD91 pKa = 3.17 GVSDD95 pKa = 3.79 EE96 pKa = 5.32 SYY98 pKa = 11.71 VSVTSDD104 pKa = 2.99 ALGTLKK110 pKa = 10.71 VGSTMGVSYY119 pKa = 10.91 GLSNHH124 pKa = 6.02 HH125 pKa = 6.93 WDD127 pKa = 3.73 VGALMDD133 pKa = 5.89 DD134 pKa = 4.17 GVHH137 pKa = 6.51 QLFAVNALGDD147 pKa = 4.41 EE148 pKa = 4.42 IDD150 pKa = 3.92 TTHH153 pKa = 5.93 NTSDD157 pKa = 3.31 GHH159 pKa = 6.49 KK160 pKa = 10.33 VIYY163 pKa = 10.24 LSPNLGGVQAGFSYY177 pKa = 11.24 GLINEE182 pKa = 4.58 TNVGSVDD189 pKa = 3.73 TLDD192 pKa = 3.81 TNNDD196 pKa = 3.09 LQYY199 pKa = 11.27 NAGIVYY205 pKa = 10.39 SADD208 pKa = 3.48 YY209 pKa = 11.0 DD210 pKa = 3.97 GLSVNVDD217 pKa = 3.17 VNYY220 pKa = 10.69 EE221 pKa = 4.13 LIEE224 pKa = 4.9 DD225 pKa = 4.14 GGLTGGGVASTTAGSEE241 pKa = 4.07 TDD243 pKa = 3.21 GTDD246 pKa = 3.04 QVKK249 pKa = 10.71 NEE251 pKa = 3.98 EE252 pKa = 3.89 SWRR255 pKa = 11.84 AGFTVAQQGLTVSASYY271 pKa = 11.32 LEE273 pKa = 4.22 TDD275 pKa = 3.23 NKK277 pKa = 9.91 GTVTGKK283 pKa = 10.49 DD284 pKa = 3.07 STAWEE289 pKa = 4.12 AGVTYY294 pKa = 8.75 KK295 pKa = 10.38 TGPYY299 pKa = 8.8 GFSVGYY305 pKa = 8.76 FNRR308 pKa = 11.84 ATEE311 pKa = 4.29 DD312 pKa = 3.46 SGLSTALEE320 pKa = 4.57 DD321 pKa = 3.68 TTDD324 pKa = 3.39 MYY326 pKa = 11.41 LVSASYY332 pKa = 11.24 NLGPGVTLAGSIVTIEE348 pKa = 4.24 TDD350 pKa = 3.55 DD351 pKa = 5.19 ASDD354 pKa = 3.6 SSPNQTDD361 pKa = 3.4 EE362 pKa = 4.12 GSNWALITGLKK373 pKa = 9.24 VHH375 pKa = 6.9 FF376 pKa = 4.81
Molecular weight: 39.3 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.698
IPC2_protein 3.834
IPC_protein 3.859
Toseland 3.63
ProMoST 4.012
Dawson 3.846
Bjellqvist 3.999
Wikipedia 3.77
Rodwell 3.681
Grimsley 3.541
Solomon 3.834
Lehninger 3.795
Nozaki 3.948
DTASelect 4.202
Thurlkill 3.681
EMBOSS 3.783
Sillero 3.973
Patrickios 1.291
IPC_peptide 3.834
IPC2_peptide 3.948
IPC2.peptide.svr19 3.852
Protein with the highest isoelectric point:
>tr|A0A1C3RHC1|A0A1C3RHC1_9PROT Putative D-alanine--D-alanine ligase domain protein OS=Candidatus Terasakiella magnetica OX=1867952 GN=MTBPR1_260007 PE=3 SV=1
MM1 pKa = 7.35 KK2 pKa = 9.43 RR3 pKa = 11.84 TYY5 pKa = 10.27 QPSKK9 pKa = 9.73 LVRR12 pKa = 11.84 KK13 pKa = 8.95 RR14 pKa = 11.84 RR15 pKa = 11.84 HH16 pKa = 4.63 GFRR19 pKa = 11.84 SRR21 pKa = 11.84 MATVAGRR28 pKa = 11.84 RR29 pKa = 11.84 VLSARR34 pKa = 11.84 RR35 pKa = 11.84 AKK37 pKa = 9.97 GRR39 pKa = 11.84 KK40 pKa = 8.91 RR41 pKa = 11.84 LSAA44 pKa = 3.96
Molecular weight: 5.18 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.448
IPC2_protein 11.155
IPC_protein 12.544
Toseland 12.705
ProMoST 13.203
Dawson 12.705
Bjellqvist 12.705
Wikipedia 13.188
Rodwell 12.384
Grimsley 12.749
Solomon 13.203
Lehninger 13.1
Nozaki 12.705
DTASelect 12.705
Thurlkill 12.705
EMBOSS 13.203
Sillero 12.705
Patrickios 12.106
IPC_peptide 13.203
IPC2_peptide 12.193
IPC2.peptide.svr19 9.089
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
3572
0
3572
1122897
21
14707
314.4
34.82
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
8.976 ± 0.062
1.063 ± 0.024
6.14 ± 0.082
6.955 ± 0.053
4.053 ± 0.029
7.384 ± 0.058
2.238 ± 0.027
6.19 ± 0.036
5.897 ± 0.069
9.66 ± 0.075
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.84 ± 0.037
3.863 ± 0.048
3.999 ± 0.048
3.693 ± 0.027
4.893 ± 0.072
6.088 ± 0.056
5.314 ± 0.086
6.92 ± 0.034
1.144 ± 0.016
2.691 ± 0.03
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here