Enterobacteria phage vB_EcoS_IME18

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Drexlerviridae; Tunavirinae; Tunavirus; Escherichia virus IME18

Average proteome isoelectric point is 6.54

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 82 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A2S1GNA7|A0A2S1GNA7_9CAUD Uncharacterized protein OS=Enterobacteria phage vB_EcoS_IME18 OX=2163886 PE=4 SV=1
MM1 pKa = 7.06MVSTDD6 pKa = 4.43KK7 pKa = 11.14FFTCTKK13 pKa = 7.72TSEE16 pKa = 4.26VFEE19 pKa = 4.93LVHH22 pKa = 6.35TDD24 pKa = 3.22NGDD27 pKa = 3.48FMHH30 pKa = 7.33DD31 pKa = 3.31GCDD34 pKa = 3.19VFIEE38 pKa = 4.49VKK40 pKa = 10.56EE41 pKa = 3.95SDD43 pKa = 3.89YY44 pKa = 11.82DD45 pKa = 3.81DD46 pKa = 3.5GVYY49 pKa = 10.86YY50 pKa = 10.88NPAVNTQFFTPIEE63 pKa = 4.26EE64 pKa = 4.39EE65 pKa = 4.39GEE67 pKa = 4.17EE68 pKa = 4.09AA69 pKa = 5.17

Molecular weight: 7.92 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A2S1GNE5|A0A2S1GNE5_9CAUD DNA adenine methyltransferase OS=Enterobacteria phage vB_EcoS_IME18 OX=2163886 PE=4 SV=1
MM1 pKa = 7.62SIQRR5 pKa = 11.84IAEE8 pKa = 4.1STGEE12 pKa = 3.45IDD14 pKa = 4.27KK15 pKa = 11.18RR16 pKa = 11.84HH17 pKa = 5.73INGNNGTRR25 pKa = 11.84RR26 pKa = 11.84GKK28 pKa = 10.02DD29 pKa = 2.81KK30 pKa = 10.89KK31 pKa = 10.07PRR33 pKa = 11.84QRR35 pKa = 11.84CGFYY39 pKa = 9.55IHH41 pKa = 7.18KK42 pKa = 9.92EE43 pKa = 3.74EE44 pKa = 4.11TRR46 pKa = 11.84AGLRR50 pKa = 11.84ARR52 pKa = 11.84LDD54 pKa = 3.51ALIEE58 pKa = 4.3YY59 pKa = 9.9YY60 pKa = 10.6GGPAACAKK68 pKa = 9.88ALKK71 pKa = 8.85VTNQTVQGWKK81 pKa = 8.54EE82 pKa = 3.77RR83 pKa = 11.84NMISWQGAEE92 pKa = 3.99AAHH95 pKa = 6.1RR96 pKa = 11.84AYY98 pKa = 10.34RR99 pKa = 11.84RR100 pKa = 11.84QGCKK104 pKa = 10.01GYY106 pKa = 9.73RR107 pKa = 11.84ASWLRR112 pKa = 11.84FDD114 pKa = 5.79LKK116 pKa = 10.99FDD118 pKa = 4.08GNGKK122 pKa = 9.4CLEE125 pKa = 4.28KK126 pKa = 10.48RR127 pKa = 11.84CKK129 pKa = 9.63NKK131 pKa = 10.41KK132 pKa = 8.86FMRR135 pKa = 11.84VVKK138 pKa = 10.61KK139 pKa = 10.74EE140 pKa = 4.29DD141 pKa = 2.97IGTTNSIFSS150 pKa = 3.71

Molecular weight: 17.23 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

82

0

82

15110

41

1139

184.3

20.64

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.167 ± 0.425

1.284 ± 0.15

6.168 ± 0.203

6.803 ± 0.199

4.004 ± 0.2

7.128 ± 0.182

1.78 ± 0.205

6.684 ± 0.21

7.121 ± 0.346

6.446 ± 0.204

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

3.223 ± 0.136

4.858 ± 0.259

3.23 ± 0.225

3.759 ± 0.232

4.924 ± 0.225

6.592 ± 0.254

5.758 ± 0.257

7.002 ± 0.229

1.416 ± 0.1

3.653 ± 0.161
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski