Amino acid dipepetide frequency for Enterobacteria phage vB_EcoS_IME18

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
7.744AlaAla: 7.744 ± 0.881
0.86AlaCys: 0.86 ± 0.326
4.037AlaAsp: 4.037 ± 0.469
4.964AlaGlu: 4.964 ± 0.732
2.052AlaPhe: 2.052 ± 0.396
5.824AlaGly: 5.824 ± 0.632
1.059AlaHis: 1.059 ± 0.258
6.685AlaIle: 6.685 ± 0.655
6.817AlaLys: 6.817 ± 1.103
6.354AlaLeu: 6.354 ± 0.736
2.846AlaMet: 2.846 ± 0.423
3.375AlaAsn: 3.375 ± 0.455
1.655AlaPro: 1.655 ± 0.317
3.375AlaGln: 3.375 ± 0.569
4.501AlaArg: 4.501 ± 0.645
5.824AlaSer: 5.824 ± 0.782
4.567AlaThr: 4.567 ± 0.745
5.361AlaVal: 5.361 ± 0.614
1.191AlaTrp: 1.191 ± 0.281
2.515AlaTyr: 2.515 ± 0.416
0.0AlaXaa: 0.0 ± 0.0
Cys
0.463CysAla: 0.463 ± 0.179
0.199CysCys: 0.199 ± 0.128
1.191CysAsp: 1.191 ± 0.265
0.794CysGlu: 0.794 ± 0.227
0.596CysPhe: 0.596 ± 0.234
1.059CysGly: 1.059 ± 0.377
0.199CysHis: 0.199 ± 0.108
0.993CysIle: 0.993 ± 0.229
0.86CysLys: 0.86 ± 0.286
0.86CysLeu: 0.86 ± 0.231
0.331CysMet: 0.331 ± 0.141
0.794CysAsn: 0.794 ± 0.209
0.463CysPro: 0.463 ± 0.217
0.463CysGln: 0.463 ± 0.188
0.529CysArg: 0.529 ± 0.187
0.794CysSer: 0.794 ± 0.251
0.728CysThr: 0.728 ± 0.258
0.86CysVal: 0.86 ± 0.279
0.199CysTrp: 0.199 ± 0.122
0.463CysTyr: 0.463 ± 0.158
0.0CysXaa: 0.0 ± 0.0
Asp
6.155AspAla: 6.155 ± 0.757
0.463AspCys: 0.463 ± 0.181
3.574AspAsp: 3.574 ± 0.631
4.898AspGlu: 4.898 ± 0.488
3.045AspPhe: 3.045 ± 0.511
6.089AspGly: 6.089 ± 0.851
1.191AspHis: 1.191 ± 0.299
3.508AspIle: 3.508 ± 0.447
4.302AspLys: 4.302 ± 0.476
3.971AspLeu: 3.971 ± 0.496
1.919AspMet: 1.919 ± 0.377
2.978AspAsn: 2.978 ± 0.554
1.655AspPro: 1.655 ± 0.322
1.721AspGln: 1.721 ± 0.393
2.118AspArg: 2.118 ± 0.42
4.236AspSer: 4.236 ± 0.591
2.449AspThr: 2.449 ± 0.391
4.434AspVal: 4.434 ± 0.597
0.596AspTrp: 0.596 ± 0.211
2.383AspTyr: 2.383 ± 0.435
0.0AspXaa: 0.0 ± 0.0
Glu
5.361GluAla: 5.361 ± 0.597
0.86GluCys: 0.86 ± 0.269
4.302GluAsp: 4.302 ± 0.513
4.898GluGlu: 4.898 ± 0.704
3.111GluPhe: 3.111 ± 0.488
3.045GluGly: 3.045 ± 0.417
0.993GluHis: 0.993 ± 0.281
6.089GluIle: 6.089 ± 0.617
4.104GluLys: 4.104 ± 0.676
4.898GluLeu: 4.898 ± 0.618
2.714GluMet: 2.714 ± 0.389
2.978GluAsn: 2.978 ± 0.384
1.721GluPro: 1.721 ± 0.466
2.581GluGln: 2.581 ± 0.494
3.442GluArg: 3.442 ± 0.566
3.905GluSer: 3.905 ± 0.443
3.773GluThr: 3.773 ± 0.558
5.493GluVal: 5.493 ± 0.69
0.728GluTrp: 0.728 ± 0.201
3.045GluTyr: 3.045 ± 0.397
0.0GluXaa: 0.0 ± 0.0
Phe
2.581PheAla: 2.581 ± 0.305
0.86PheCys: 0.86 ± 0.249
2.846PheAsp: 2.846 ± 0.436
3.309PheGlu: 3.309 ± 0.469
1.522PhePhe: 1.522 ± 0.426
3.375PheGly: 3.375 ± 0.535
0.662PheHis: 0.662 ± 0.259
2.714PheIle: 2.714 ± 0.393
2.978PheLys: 2.978 ± 0.448
1.787PheLeu: 1.787 ± 0.349
1.655PheMet: 1.655 ± 0.321
2.515PheAsn: 2.515 ± 0.349
1.456PhePro: 1.456 ± 0.308
1.191PheGln: 1.191 ± 0.319
1.522PheArg: 1.522 ± 0.295
2.317PheSer: 2.317 ± 0.359
2.78PheThr: 2.78 ± 0.404
2.118PheVal: 2.118 ± 0.359
0.794PheTrp: 0.794 ± 0.203
1.059PheTyr: 1.059 ± 0.245
0.0PheXaa: 0.0 ± 0.0
Gly
4.964GlyAla: 4.964 ± 0.736
1.655GlyCys: 1.655 ± 0.34
4.898GlyAsp: 4.898 ± 0.562
4.633GlyGlu: 4.633 ± 0.577
2.25GlyPhe: 2.25 ± 0.418
5.758GlyGly: 5.758 ± 0.822
1.324GlyHis: 1.324 ± 0.376
3.111GlyIle: 3.111 ± 0.437
5.957GlyLys: 5.957 ± 0.592
4.368GlyLeu: 4.368 ± 0.48
2.78GlyMet: 2.78 ± 0.492
3.574GlyAsn: 3.574 ± 0.5
0.529GlyPro: 0.529 ± 0.256
2.184GlyGln: 2.184 ± 0.355
3.243GlyArg: 3.243 ± 0.426
5.229GlySer: 5.229 ± 0.625
3.773GlyThr: 3.773 ± 0.419
5.957GlyVal: 5.957 ± 0.675
1.721GlyTrp: 1.721 ± 0.267
3.375GlyTyr: 3.375 ± 0.52
0.0GlyXaa: 0.0 ± 0.0
His
0.993HisAla: 0.993 ± 0.284
0.132HisCys: 0.132 ± 0.084
1.258HisAsp: 1.258 ± 0.274
1.324HisGlu: 1.324 ± 0.291
0.794HisPhe: 0.794 ± 0.207
1.125HisGly: 1.125 ± 0.27
0.529HisHis: 0.529 ± 0.164
1.191HisIle: 1.191 ± 0.349
1.39HisLys: 1.39 ± 0.328
1.125HisLeu: 1.125 ± 0.319
0.529HisMet: 0.529 ± 0.199
0.794HisAsn: 0.794 ± 0.232
0.794HisPro: 0.794 ± 0.233
0.529HisGln: 0.529 ± 0.201
0.993HisArg: 0.993 ± 0.307
1.258HisSer: 1.258 ± 0.318
1.059HisThr: 1.059 ± 0.302
0.993HisVal: 0.993 ± 0.297
0.199HisTrp: 0.199 ± 0.123
0.794HisTyr: 0.794 ± 0.271
0.0HisXaa: 0.0 ± 0.0
Ile
6.685IleAla: 6.685 ± 0.594
0.794IleCys: 0.794 ± 0.217
4.699IleAsp: 4.699 ± 0.521
4.964IleGlu: 4.964 ± 0.497
2.449IlePhe: 2.449 ± 0.411
4.434IleGly: 4.434 ± 0.556
0.86IleHis: 0.86 ± 0.262
4.17IleIle: 4.17 ± 0.452
5.229IleLys: 5.229 ± 0.667
3.375IleLeu: 3.375 ± 0.602
1.787IleMet: 1.787 ± 0.375
3.773IleAsn: 3.773 ± 0.483
2.052IlePro: 2.052 ± 0.38
2.383IleGln: 2.383 ± 0.386
2.647IleArg: 2.647 ± 0.424
4.633IleSer: 4.633 ± 0.631
5.229IleThr: 5.229 ± 0.592
3.508IleVal: 3.508 ± 0.524
0.794IleTrp: 0.794 ± 0.202
2.383IleTyr: 2.383 ± 0.365
0.0IleXaa: 0.0 ± 0.0
Lys
6.486LysAla: 6.486 ± 0.97
1.059LysCys: 1.059 ± 0.297
4.037LysAsp: 4.037 ± 0.579
5.957LysGlu: 5.957 ± 0.646
2.978LysPhe: 2.978 ± 0.717
4.567LysGly: 4.567 ± 0.565
1.655LysHis: 1.655 ± 0.345
4.633LysIle: 4.633 ± 0.606
4.633LysLys: 4.633 ± 0.56
4.964LysLeu: 4.964 ± 0.624
2.78LysMet: 2.78 ± 0.409
3.177LysAsn: 3.177 ± 0.386
2.78LysPro: 2.78 ± 0.485
2.912LysGln: 2.912 ± 0.507
3.773LysArg: 3.773 ± 0.49
3.442LysSer: 3.442 ± 0.473
3.839LysThr: 3.839 ± 0.441
4.699LysVal: 4.699 ± 0.73
0.662LysTrp: 0.662 ± 0.196
2.184LysTyr: 2.184 ± 0.331
0.0LysXaa: 0.0 ± 0.0
Leu
5.096LeuAla: 5.096 ± 0.707
0.596LeuCys: 0.596 ± 0.207
4.037LeuAsp: 4.037 ± 0.494
3.508LeuGlu: 3.508 ± 0.504
2.184LeuPhe: 2.184 ± 0.371
3.905LeuGly: 3.905 ± 0.516
1.522LeuHis: 1.522 ± 0.341
4.434LeuIle: 4.434 ± 0.551
4.832LeuLys: 4.832 ± 0.691
4.17LeuLeu: 4.17 ± 0.491
1.721LeuMet: 1.721 ± 0.281
3.045LeuAsn: 3.045 ± 0.486
3.045LeuPro: 3.045 ± 0.357
1.721LeuGln: 1.721 ± 0.302
3.375LeuArg: 3.375 ± 0.475
4.964LeuSer: 4.964 ± 0.587
4.964LeuThr: 4.964 ± 0.51
4.368LeuVal: 4.368 ± 0.607
0.993LeuTrp: 0.993 ± 0.278
1.986LeuTyr: 1.986 ± 0.347
0.0LeuXaa: 0.0 ± 0.0
Met
2.449MetAla: 2.449 ± 0.359
0.397MetCys: 0.397 ± 0.149
1.39MetAsp: 1.39 ± 0.314
2.449MetGlu: 2.449 ± 0.42
1.787MetPhe: 1.787 ± 0.321
0.86MetGly: 0.86 ± 0.267
0.596MetHis: 0.596 ± 0.207
2.78MetIle: 2.78 ± 0.417
2.978MetLys: 2.978 ± 0.488
2.184MetLeu: 2.184 ± 0.439
1.787MetMet: 1.787 ± 0.377
1.324MetAsn: 1.324 ± 0.252
0.993MetPro: 0.993 ± 0.212
1.456MetGln: 1.456 ± 0.308
1.919MetArg: 1.919 ± 0.391
2.515MetSer: 2.515 ± 0.411
1.456MetThr: 1.456 ± 0.31
1.919MetVal: 1.919 ± 0.344
0.265MetTrp: 0.265 ± 0.129
0.728MetTyr: 0.728 ± 0.224
0.0MetXaa: 0.0 ± 0.0
Asn
4.302AsnAla: 4.302 ± 0.733
0.397AsnCys: 0.397 ± 0.171
3.375AsnAsp: 3.375 ± 0.372
2.714AsnGlu: 2.714 ± 0.405
2.052AsnPhe: 2.052 ± 0.384
4.368AsnGly: 4.368 ± 0.695
0.993AsnHis: 0.993 ± 0.274
3.111AsnIle: 3.111 ± 0.406
3.111AsnLys: 3.111 ± 0.517
2.449AsnLeu: 2.449 ± 0.521
1.059AsnMet: 1.059 ± 0.224
2.25AsnAsn: 2.25 ± 0.379
2.118AsnPro: 2.118 ± 0.322
2.515AsnGln: 2.515 ± 0.366
1.721AsnArg: 1.721 ± 0.32
3.839AsnSer: 3.839 ± 0.473
2.118AsnThr: 2.118 ± 0.355
3.574AsnVal: 3.574 ± 0.552
0.728AsnTrp: 0.728 ± 0.195
1.787AsnTyr: 1.787 ± 0.355
0.0AsnXaa: 0.0 ± 0.0
Pro
1.919ProAla: 1.919 ± 0.379
0.397ProCys: 0.397 ± 0.189
1.919ProAsp: 1.919 ± 0.421
2.78ProGlu: 2.78 ± 0.609
1.191ProPhe: 1.191 ± 0.274
2.647ProGly: 2.647 ± 0.439
0.596ProHis: 0.596 ± 0.181
1.787ProIle: 1.787 ± 0.364
1.456ProLys: 1.456 ± 0.368
1.588ProLeu: 1.588 ± 0.262
1.125ProMet: 1.125 ± 0.261
2.184ProAsn: 2.184 ± 0.366
1.191ProPro: 1.191 ± 0.443
1.125ProGln: 1.125 ± 0.309
1.324ProArg: 1.324 ± 0.33
1.919ProSer: 1.919 ± 0.336
1.853ProThr: 1.853 ± 0.32
3.375ProVal: 3.375 ± 0.44
0.463ProTrp: 0.463 ± 0.168
1.456ProTyr: 1.456 ± 0.284
0.0ProXaa: 0.0 ± 0.0
Gln
3.177GlnAla: 3.177 ± 0.474
0.331GlnCys: 0.331 ± 0.162
1.588GlnAsp: 1.588 ± 0.335
2.383GlnGlu: 2.383 ± 0.446
1.059GlnPhe: 1.059 ± 0.261
2.449GlnGly: 2.449 ± 0.41
0.529GlnHis: 0.529 ± 0.167
2.714GlnIle: 2.714 ± 0.386
2.118GlnLys: 2.118 ± 0.382
3.442GlnLeu: 3.442 ± 0.564
0.993GlnMet: 0.993 ± 0.234
2.25GlnAsn: 2.25 ± 0.427
1.125GlnPro: 1.125 ± 0.31
2.052GlnGln: 2.052 ± 0.443
1.787GlnArg: 1.787 ± 0.35
2.515GlnSer: 2.515 ± 0.481
1.655GlnThr: 1.655 ± 0.346
3.111GlnVal: 3.111 ± 0.401
0.728GlnTrp: 0.728 ± 0.242
1.588GlnTyr: 1.588 ± 0.322
0.0GlnXaa: 0.0 ± 0.0
Arg
3.375ArgAla: 3.375 ± 0.519
0.927ArgCys: 0.927 ± 0.364
2.515ArgAsp: 2.515 ± 0.443
3.971ArgGlu: 3.971 ± 0.48
2.184ArgPhe: 2.184 ± 0.402
2.978ArgGly: 2.978 ± 0.388
0.86ArgHis: 0.86 ± 0.244
3.045ArgIle: 3.045 ± 0.499
3.773ArgLys: 3.773 ± 0.415
3.64ArgLeu: 3.64 ± 0.395
1.324ArgMet: 1.324 ± 0.3
2.052ArgAsn: 2.052 ± 0.38
1.655ArgPro: 1.655 ± 0.413
1.456ArgGln: 1.456 ± 0.316
3.177ArgArg: 3.177 ± 0.536
2.846ArgSer: 2.846 ± 0.448
1.522ArgThr: 1.522 ± 0.363
4.699ArgVal: 4.699 ± 0.599
0.794ArgTrp: 0.794 ± 0.255
2.449ArgTyr: 2.449 ± 0.399
0.0ArgXaa: 0.0 ± 0.0
Ser
5.56SerAla: 5.56 ± 0.856
0.794SerCys: 0.794 ± 0.244
4.037SerAsp: 4.037 ± 0.466
4.037SerGlu: 4.037 ± 0.424
3.243SerPhe: 3.243 ± 0.393
6.354SerGly: 6.354 ± 0.525
0.794SerHis: 0.794 ± 0.234
4.037SerIle: 4.037 ± 0.407
3.773SerLys: 3.773 ± 0.443
5.626SerLeu: 5.626 ± 0.7
1.655SerMet: 1.655 ± 0.309
2.449SerAsn: 2.449 ± 0.611
1.986SerPro: 1.986 ± 0.302
2.846SerGln: 2.846 ± 0.373
3.375SerArg: 3.375 ± 0.34
3.706SerSer: 3.706 ± 0.464
3.971SerThr: 3.971 ± 0.521
4.501SerVal: 4.501 ± 0.521
0.993SerTrp: 0.993 ± 0.294
2.184SerTyr: 2.184 ± 0.408
0.0SerXaa: 0.0 ± 0.0
Thr
4.832ThrAla: 4.832 ± 0.603
1.191ThrCys: 1.191 ± 0.273
3.706ThrAsp: 3.706 ± 0.505
3.243ThrGlu: 3.243 ± 0.429
2.912ThrPhe: 2.912 ± 0.412
4.964ThrGly: 4.964 ± 0.459
0.993ThrHis: 0.993 ± 0.238
3.971ThrIle: 3.971 ± 0.46
3.574ThrLys: 3.574 ± 0.561
3.375ThrLeu: 3.375 ± 0.446
1.522ThrMet: 1.522 ± 0.323
2.581ThrAsn: 2.581 ± 0.538
2.978ThrPro: 2.978 ± 0.527
2.317ThrGln: 2.317 ± 0.364
1.986ThrArg: 1.986 ± 0.387
3.64ThrSer: 3.64 ± 0.366
3.375ThrThr: 3.375 ± 0.587
3.971ThrVal: 3.971 ± 0.576
0.794ThrTrp: 0.794 ± 0.208
1.655ThrTyr: 1.655 ± 0.264
0.0ThrXaa: 0.0 ± 0.0
Val
5.427ValAla: 5.427 ± 0.602
0.463ValCys: 0.463 ± 0.188
4.633ValAsp: 4.633 ± 0.519
4.434ValGlu: 4.434 ± 0.662
2.912ValPhe: 2.912 ± 0.501
3.64ValGly: 3.64 ± 0.602
1.456ValHis: 1.456 ± 0.331
4.501ValIle: 4.501 ± 0.618
6.089ValLys: 6.089 ± 0.75
3.574ValLeu: 3.574 ± 0.438
1.721ValMet: 1.721 ± 0.354
3.773ValAsn: 3.773 ± 0.561
2.449ValPro: 2.449 ± 0.485
2.714ValGln: 2.714 ± 0.506
4.17ValArg: 4.17 ± 0.583
5.162ValSer: 5.162 ± 0.519
4.898ValThr: 4.898 ± 0.553
4.699ValVal: 4.699 ± 0.637
1.059ValTrp: 1.059 ± 0.212
2.25ValTyr: 2.25 ± 0.419
0.0ValXaa: 0.0 ± 0.0
Trp
1.125TrpAla: 1.125 ± 0.312
0.199TrpCys: 0.199 ± 0.112
0.86TrpAsp: 0.86 ± 0.233
0.662TrpGlu: 0.662 ± 0.22
0.463TrpPhe: 0.463 ± 0.223
0.927TrpGly: 0.927 ± 0.225
0.331TrpHis: 0.331 ± 0.15
0.662TrpIle: 0.662 ± 0.18
1.059TrpLys: 1.059 ± 0.29
0.927TrpLeu: 0.927 ± 0.231
0.463TrpMet: 0.463 ± 0.157
0.86TrpAsn: 0.86 ± 0.245
0.265TrpPro: 0.265 ± 0.162
0.596TrpGln: 0.596 ± 0.157
1.059TrpArg: 1.059 ± 0.302
0.927TrpSer: 0.927 ± 0.249
1.324TrpThr: 1.324 ± 0.302
0.728TrpVal: 0.728 ± 0.168
0.199TrpTrp: 0.199 ± 0.121
0.529TrpTyr: 0.529 ± 0.174
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.978TyrAla: 2.978 ± 0.432
0.265TyrCys: 0.265 ± 0.117
2.78TyrAsp: 2.78 ± 0.408
2.118TyrGlu: 2.118 ± 0.367
1.258TyrPhe: 1.258 ± 0.283
2.912TyrGly: 2.912 ± 0.348
0.662TyrHis: 0.662 ± 0.184
2.714TyrIle: 2.714 ± 0.446
2.184TyrLys: 2.184 ± 0.35
1.787TyrLeu: 1.787 ± 0.369
1.456TyrMet: 1.456 ± 0.347
1.853TyrAsn: 1.853 ± 0.348
1.39TyrPro: 1.39 ± 0.302
1.456TyrGln: 1.456 ± 0.281
2.581TyrArg: 2.581 ± 0.382
2.25TyrSer: 2.25 ± 0.398
2.25TyrThr: 2.25 ± 0.428
1.655TyrVal: 1.655 ± 0.314
0.265TyrTrp: 0.265 ± 0.149
1.721TyrTyr: 1.721 ± 0.318
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 82 proteins (15110 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski