Capybara microvirus Cap3_SP_264
Average proteome isoelectric point is 6.36
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 6 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A4P8W5J5|A0A4P8W5J5_9VIRU Uncharacterized protein OS=Capybara microvirus Cap3_SP_264 OX=2585422 PE=4 SV=1
MM1 pKa = 7.35 SKK3 pKa = 10.58 KK4 pKa = 9.99 EE5 pKa = 4.07 AIKK8 pKa = 10.87 NDD10 pKa = 3.48 DD11 pKa = 3.66 EE12 pKa = 4.55 LRR14 pKa = 11.84 VYY16 pKa = 10.77 LEE18 pKa = 4.09 SLTPDD23 pKa = 3.33 EE24 pKa = 4.71 LRR26 pKa = 11.84 DD27 pKa = 3.76 VKK29 pKa = 11.02 SVLSYY34 pKa = 10.16 QVEE37 pKa = 4.33 MTKK40 pKa = 10.7 SNKK43 pKa = 9.83 DD44 pKa = 3.02 SAANDD49 pKa = 3.44 LQLFKK54 pKa = 11.29 DD55 pKa = 3.41 NEE57 pKa = 4.37 IYY59 pKa = 10.73 FKK61 pKa = 10.6 IPYY64 pKa = 9.77 AEE66 pKa = 4.18 SLLLYY71 pKa = 7.24 WTTAYY76 pKa = 10.5 QSAKK80 pKa = 11.04 SMLEE84 pKa = 3.66 KK85 pKa = 10.58 FNQFLILL92 pKa = 3.93
Molecular weight: 10.81 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.909
IPC2_protein 5.016
IPC_protein 4.813
Toseland 4.66
ProMoST 4.914
Dawson 4.749
Bjellqvist 4.902
Wikipedia 4.622
Rodwell 4.647
Grimsley 4.571
Solomon 4.749
Lehninger 4.698
Nozaki 4.863
DTASelect 5.003
Thurlkill 4.66
EMBOSS 4.635
Sillero 4.914
Patrickios 4.088
IPC_peptide 4.749
IPC2_peptide 4.902
IPC2.peptide.svr19 4.953
Protein with the highest isoelectric point:
>tr|A0A4P8W5S2|A0A4P8W5S2_9VIRU Replication initiator protein OS=Capybara microvirus Cap3_SP_264 OX=2585422 PE=4 SV=1
MM1 pKa = 7.09 HH2 pKa = 7.12 QISNSRR8 pKa = 11.84 LIYY11 pKa = 9.82 QIKK14 pKa = 10.11 KK15 pKa = 7.64 YY16 pKa = 9.8 NQMVHH21 pKa = 7.32 RR22 pKa = 11.84 INPLKK27 pKa = 10.32 PYY29 pKa = 9.89 QVYY32 pKa = 9.21 QAPVGSSSLFVDD44 pKa = 4.04 EE45 pKa = 4.93 FSTYY49 pKa = 10.8 EE50 pKa = 3.93 EE51 pKa = 4.11 ALKK54 pKa = 10.41 CAKK57 pKa = 10.23 SIAKK61 pKa = 9.3 AWCNDD66 pKa = 3.03 YY67 pKa = 9.31 TLIIDD72 pKa = 3.75 SQGFSTRR79 pKa = 11.84 LEE81 pKa = 4.14 YY82 pKa = 10.86 VFPKK86 pKa = 10.68 SNPLKK91 pKa = 10.69 NKK93 pKa = 8.8 VKK95 pKa = 10.64 SYY97 pKa = 9.67 VV98 pKa = 3.14
Molecular weight: 11.4 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 8.775
IPC2_protein 8.887
IPC_protein 8.814
Toseland 9.282
ProMoST 9.165
Dawson 9.619
Bjellqvist 9.399
Wikipedia 9.809
Rodwell 9.809
Grimsley 9.706
Solomon 9.677
Lehninger 9.633
Nozaki 9.414
DTASelect 9.355
Thurlkill 9.443
EMBOSS 9.736
Sillero 9.575
Patrickios 4.202
IPC_peptide 9.663
IPC2_peptide 8.112
IPC2.peptide.svr19 7.809
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
6
0
6
2082
88
898
347.0
39.59
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
5.812 ± 0.85
1.393 ± 0.474
6.532 ± 0.464
4.563 ± 0.613
6.244 ± 0.949
5.043 ± 1.068
1.969 ± 0.463
5.668 ± 0.44
5.764 ± 1.167
8.934 ± 0.774
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.921 ± 0.438
7.589 ± 0.753
4.515 ± 0.436
4.995 ± 0.837
4.515 ± 0.671
9.126 ± 0.622
4.275 ± 0.518
5.139 ± 0.664
0.913 ± 0.074
5.091 ± 0.625
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here