Amino acid dipepetide frequency for unidentified eubacterium SCB49

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	5.02AlaAla: 5.02 ± 0.102	0.577AlaCys: 0.577 ± 0.027	3.475AlaAsp: 3.475 ± 0.079	4.03AlaGlu: 4.03 ± 0.076	3.466AlaPhe: 3.466 ± 0.061	4.381AlaGly: 4.381 ± 0.093	1.158AlaHis: 1.158 ± 0.038	5.867AlaIle: 5.867 ± 0.087	4.689AlaLys: 4.689 ± 0.083	6.543AlaLeu: 6.543 ± 0.085	1.74AlaMet: 1.74 ± 0.042	3.618AlaAsn: 3.618 ± 0.079	2.142AlaPro: 2.142 ± 0.057	2.673AlaGln: 2.673 ± 0.057	2.076AlaArg: 2.076 ± 0.051	4.41AlaSer: 4.41 ± 0.085	4.45AlaThr: 4.45 ± 0.109	4.507AlaVal: 4.507 ± 0.075	0.629AlaTrp: 0.629 ± 0.029	2.494AlaTyr: 2.494 ± 0.055	0.0AlaXaa: 0.0 ± 0.0
Cys	0.49CysAla: 0.49 ± 0.023	0.096CysCys: 0.096 ± 0.01	0.647CysAsp: 0.647 ± 0.08	0.481CysGlu: 0.481 ± 0.027	0.391CysPhe: 0.391 ± 0.021	0.656CysGly: 0.656 ± 0.029	0.152CysHis: 0.152 ± 0.014	0.587CysIle: 0.587 ± 0.025	0.437CysLys: 0.437 ± 0.023	0.661CysLeu: 0.661 ± 0.028	0.117CysMet: 0.117 ± 0.009	0.44CysAsn: 0.44 ± 0.024	0.322CysPro: 0.322 ± 0.022	0.226CysGln: 0.226 ± 0.018	0.161CysArg: 0.161 ± 0.012	0.523CysSer: 0.523 ± 0.027	0.445CysThr: 0.445 ± 0.024	0.479CysVal: 0.479 ± 0.025	0.049CysTrp: 0.049 ± 0.008	0.294CysTyr: 0.294 ± 0.02	0.0CysXaa: 0.0 ± 0.0
Asp	4.216AspAla: 4.216 ± 0.078	0.518AspCys: 0.518 ± 0.053	3.211AspAsp: 3.211 ± 0.074	3.775AspGlu: 3.775 ± 0.084	3.664AspPhe: 3.664 ± 0.074	4.223AspGly: 4.223 ± 0.169	0.935AspHis: 0.935 ± 0.031	4.554AspIle: 4.554 ± 0.077	4.16AspLys: 4.16 ± 0.077	5.195AspLeu: 5.195 ± 0.091	1.082AspMet: 1.082 ± 0.033	3.262AspAsn: 3.262 ± 0.067	1.89AspPro: 1.89 ± 0.086	1.645AspGln: 1.645 ± 0.042	1.9AspArg: 1.9 ± 0.047	3.246AspSer: 3.246 ± 0.064	3.432AspThr: 3.432 ± 0.065	4.036AspVal: 4.036 ± 0.082	0.722AspTrp: 0.722 ± 0.028	2.835AspTyr: 2.835 ± 0.063	0.0AspXaa: 0.0 ± 0.0
Glu	4.881GluAla: 4.881 ± 0.09	0.37GluCys: 0.37 ± 0.021	3.864GluAsp: 3.864 ± 0.068	4.902GluGlu: 4.902 ± 0.094	2.721GluPhe: 2.721 ± 0.06	3.972GluGly: 3.972 ± 0.079	1.129GluHis: 1.129 ± 0.034	5.743GluIle: 5.743 ± 0.085	5.698GluLys: 5.698 ± 0.09	5.835GluLeu: 5.835 ± 0.083	1.741GluMet: 1.741 ± 0.045	4.713GluAsn: 4.713 ± 0.08	1.488GluPro: 1.488 ± 0.044	2.345GluGln: 2.345 ± 0.048	2.415GluArg: 2.415 ± 0.057	3.191GluSer: 3.191 ± 0.06	4.146GluThr: 4.146 ± 0.069	4.497GluVal: 4.497 ± 0.084	0.569GluTrp: 0.569 ± 0.032	2.397GluTyr: 2.397 ± 0.062	0.0GluXaa: 0.0 ± 0.0
Phe	2.934PheAla: 2.934 ± 0.056	0.458PheCys: 0.458 ± 0.022	3.299PheAsp: 3.299 ± 0.068	3.339PheGlu: 3.339 ± 0.063	2.723PhePhe: 2.723 ± 0.071	3.474PheGly: 3.474 ± 0.067	0.752PheHis: 0.752 ± 0.028	3.905PheIle: 3.905 ± 0.071	3.901PheLys: 3.901 ± 0.075	4.495PheLeu: 4.495 ± 0.098	1.057PheMet: 1.057 ± 0.036	3.271PheAsn: 3.271 ± 0.068	1.7PhePro: 1.7 ± 0.044	1.381PheGln: 1.381 ± 0.041	1.424PheArg: 1.424 ± 0.037	3.921PheSer: 3.921 ± 0.073	3.493PheThr: 3.493 ± 0.075	3.09PheVal: 3.09 ± 0.068	0.55PheTrp: 0.55 ± 0.025	2.078PheTyr: 2.078 ± 0.048	0.0PheXaa: 0.0 ± 0.0
Gly	4.674GlyAla: 4.674 ± 0.116	0.583GlyCys: 0.583 ± 0.032	3.933GlyAsp: 3.933 ± 0.116	3.585GlyGlu: 3.585 ± 0.065	3.538GlyPhe: 3.538 ± 0.06	4.759GlyGly: 4.759 ± 0.114	1.084GlyHis: 1.084 ± 0.035	5.113GlyIle: 5.113 ± 0.088	4.421GlyLys: 4.421 ± 0.072	5.657GlyLeu: 5.657 ± 0.092	1.571GlyMet: 1.571 ± 0.049	3.668GlyAsn: 3.668 ± 0.069	1.437GlyPro: 1.437 ± 0.066	1.877GlyGln: 1.877 ± 0.058	2.095GlyArg: 2.095 ± 0.057	3.984GlySer: 3.984 ± 0.069	4.212GlyThr: 4.212 ± 0.089	4.834GlyVal: 4.834 ± 0.087	0.711GlyTrp: 0.711 ± 0.028	2.642GlyTyr: 2.642 ± 0.055	0.0GlyXaa: 0.0 ± 0.0
His	0.915HisAla: 0.915 ± 0.034	0.17HisCys: 0.17 ± 0.014	0.794HisAsp: 0.794 ± 0.027	0.85HisGlu: 0.85 ± 0.036	1.114HisPhe: 1.114 ± 0.037	0.962HisGly: 0.962 ± 0.028	0.454HisHis: 0.454 ± 0.024	1.37HisIle: 1.37 ± 0.04	1.276HisLys: 1.276 ± 0.038	1.742HisLeu: 1.742 ± 0.046	0.289HisMet: 0.289 ± 0.017	0.98HisAsn: 0.98 ± 0.031	0.875HisPro: 0.875 ± 0.031	0.638HisGln: 0.638 ± 0.027	0.622HisArg: 0.622 ± 0.024	0.954HisSer: 0.954 ± 0.033	1.054HisThr: 1.054 ± 0.037	0.898HisVal: 0.898 ± 0.037	0.214HisTrp: 0.214 ± 0.017	0.809HisTyr: 0.809 ± 0.029	0.0HisXaa: 0.0 ± 0.0
Ile	6.067IleAla: 6.067 ± 0.092	0.616IleCys: 0.616 ± 0.027	5.188IleAsp: 5.188 ± 0.082	5.495IleGlu: 5.495 ± 0.082	3.514IlePhe: 3.514 ± 0.077	4.923IleGly: 4.923 ± 0.083	1.268IleHis: 1.268 ± 0.035	5.917IleIle: 5.917 ± 0.103	5.826IleLys: 5.826 ± 0.084	6.84IleLeu: 6.84 ± 0.116	1.334IleMet: 1.334 ± 0.043	4.563IleAsn: 4.563 ± 0.077	3.099IlePro: 3.099 ± 0.063	2.292IleGln: 2.292 ± 0.051	2.277IleArg: 2.277 ± 0.049	5.805IleSer: 5.805 ± 0.076	5.294IleThr: 5.294 ± 0.087	4.849IleVal: 4.849 ± 0.08	0.65IleTrp: 0.65 ± 0.027	2.726IleTyr: 2.726 ± 0.06	0.0IleXaa: 0.0 ± 0.0
Lys	4.885LysAla: 4.885 ± 0.087	0.306LysCys: 0.306 ± 0.02	4.412LysAsp: 4.412 ± 0.081	6.21LysGlu: 6.21 ± 0.088	2.684LysPhe: 2.684 ± 0.06	4.395LysGly: 4.395 ± 0.083	1.383LysHis: 1.383 ± 0.039	5.928LysIle: 5.928 ± 0.105	7.065LysLys: 7.065 ± 0.11	6.178LysLeu: 6.178 ± 0.101	2.217LysMet: 2.217 ± 0.051	5.127LysAsn: 5.127 ± 0.096	2.244LysPro: 2.244 ± 0.058	2.775LysGln: 2.775 ± 0.053	2.799LysArg: 2.799 ± 0.057	4.266LysSer: 4.266 ± 0.076	4.815LysThr: 4.815 ± 0.082	4.534LysVal: 4.534 ± 0.079	0.698LysTrp: 0.698 ± 0.028	2.882LysTyr: 2.882 ± 0.061	0.0LysXaa: 0.0 ± 0.0
Leu	6.093LeuAla: 6.093 ± 0.092	0.665LeuCys: 0.665 ± 0.033	5.607LeuAsp: 5.607 ± 0.083	6.098LeuGlu: 6.098 ± 0.083	4.91LeuPhe: 4.91 ± 0.097	5.787LeuGly: 5.787 ± 0.086	1.54LeuHis: 1.54 ± 0.042	6.712LeuIle: 6.712 ± 0.106	7.502LeuLys: 7.502 ± 0.113	8.858LeuLeu: 8.858 ± 0.139	1.957LeuMet: 1.957 ± 0.044	5.363LeuAsn: 5.363 ± 0.081	3.32LeuPro: 3.32 ± 0.062	3.183LeuGln: 3.183 ± 0.065	3.1LeuArg: 3.1 ± 0.067	6.476LeuSer: 6.476 ± 0.096	5.28LeuThr: 5.28 ± 0.081	5.503LeuVal: 5.503 ± 0.087	0.714LeuTrp: 0.714 ± 0.029	3.062LeuTyr: 3.062 ± 0.063	0.0LeuXaa: 0.0 ± 0.0
Met	1.684MetAla: 1.684 ± 0.047	0.149MetCys: 0.149 ± 0.013	1.16MetAsp: 1.16 ± 0.035	1.327MetGlu: 1.327 ± 0.04	0.8MetPhe: 0.8 ± 0.031	1.388MetGly: 1.388 ± 0.041	0.39MetHis: 0.39 ± 0.025	1.495MetIle: 1.495 ± 0.048	2.083MetLys: 2.083 ± 0.047	2.042MetLeu: 2.042 ± 0.051	0.586MetMet: 0.586 ± 0.027	1.216MetAsn: 1.216 ± 0.032	0.845MetPro: 0.845 ± 0.031	0.814MetGln: 0.814 ± 0.03	0.812MetArg: 0.812 ± 0.029	1.428MetSer: 1.428 ± 0.032	1.215MetThr: 1.215 ± 0.033	1.337MetVal: 1.337 ± 0.043	0.158MetTrp: 0.158 ± 0.013	0.755MetTyr: 0.755 ± 0.029	0.0MetXaa: 0.0 ± 0.0
Asn	3.898AsnAla: 3.898 ± 0.066	0.477AsnCys: 0.477 ± 0.035	3.441AsnAsp: 3.441 ± 0.071	3.912AsnGlu: 3.912 ± 0.058	2.886AsnPhe: 2.886 ± 0.056	3.963AsnGly: 3.963 ± 0.094	0.98AsnHis: 0.98 ± 0.033	4.751AsnIle: 4.751 ± 0.079	4.405AsnLys: 4.405 ± 0.075	5.105AsnLeu: 5.105 ± 0.085	1.123AsnMet: 1.123 ± 0.036	3.79AsnAsn: 3.79 ± 0.087	2.599AsnPro: 2.599 ± 0.051	1.992AsnGln: 1.992 ± 0.051	1.94AsnArg: 1.94 ± 0.047	3.594AsnSer: 3.594 ± 0.076	4.011AsnThr: 4.011 ± 0.085	3.61AsnVal: 3.61 ± 0.07	0.677AsnTrp: 0.677 ± 0.028	2.658AsnTyr: 2.658 ± 0.062	0.0AsnXaa: 0.0 ± 0.0
Pro	2.013ProAla: 2.013 ± 0.05	0.276ProCys: 0.276 ± 0.046	2.035ProAsp: 2.035 ± 0.057	2.703ProGlu: 2.703 ± 0.056	1.874ProPhe: 1.874 ± 0.051	1.885ProGly: 1.885 ± 0.056	0.546ProHis: 0.546 ± 0.027	2.585ProIle: 2.585 ± 0.053	2.418ProLys: 2.418 ± 0.059	2.999ProLeu: 2.999 ± 0.061	0.644ProMet: 0.644 ± 0.028	2.122ProAsn: 2.122 ± 0.051	0.82ProPro: 0.82 ± 0.039	1.122ProGln: 1.122 ± 0.05	0.861ProArg: 0.861 ± 0.03	2.239ProSer: 2.239 ± 0.046	2.094ProThr: 2.094 ± 0.048	2.375ProVal: 2.375 ± 0.054	0.3ProTrp: 0.3 ± 0.018	1.341ProTyr: 1.341 ± 0.036	0.0ProXaa: 0.0 ± 0.0
Gln	2.123GlnAla: 2.123 ± 0.048	0.186GlnCys: 0.186 ± 0.013	1.9GlnAsp: 1.9 ± 0.061	2.439GlnGlu: 2.439 ± 0.058	1.636GlnPhe: 1.636 ± 0.045	1.921GlnGly: 1.921 ± 0.05	0.601GlnHis: 0.601 ± 0.025	2.498GlnIle: 2.498 ± 0.043	2.64GlnLys: 2.64 ± 0.057	3.458GlnLeu: 3.458 ± 0.06	0.804GlnMet: 0.804 ± 0.028	2.004GlnAsn: 2.004 ± 0.049	0.994GlnPro: 0.994 ± 0.03	1.363GlnGln: 1.363 ± 0.049	1.207GlnArg: 1.207 ± 0.036	1.85GlnSer: 1.85 ± 0.041	1.944GlnThr: 1.944 ± 0.05	2.018GlnVal: 2.018 ± 0.047	0.351GlnTrp: 0.351 ± 0.018	1.295GlnTyr: 1.295 ± 0.037	0.0GlnXaa: 0.0 ± 0.0
Arg	2.127ArgAla: 2.127 ± 0.048	0.184ArgCys: 0.184 ± 0.014	1.82ArgAsp: 1.82 ± 0.049	2.162ArgGlu: 2.162 ± 0.055	1.822ArgPhe: 1.822 ± 0.043	1.917ArgGly: 1.917 ± 0.055	0.557ArgHis: 0.557 ± 0.024	2.576ArgIle: 2.576 ± 0.058	2.686ArgLys: 2.686 ± 0.059	3.207ArgLeu: 3.207 ± 0.058	0.835ArgMet: 0.835 ± 0.026	1.942ArgAsn: 1.942 ± 0.044	1.045ArgPro: 1.045 ± 0.039	1.054ArgGln: 1.054 ± 0.033	1.246ArgArg: 1.246 ± 0.038	1.837ArgSer: 1.837 ± 0.05	1.745ArgThr: 1.745 ± 0.043	2.128ArgVal: 2.128 ± 0.05	0.357ArgTrp: 0.357 ± 0.02	1.44ArgTyr: 1.44 ± 0.042	0.0ArgXaa: 0.0 ± 0.0
Ser	3.823SerAla: 3.823 ± 0.061	0.649SerCys: 0.649 ± 0.031	3.377SerAsp: 3.377 ± 0.065	4.537SerGlu: 4.537 ± 0.07	3.893SerPhe: 3.893 ± 0.071	4.441SerGly: 4.441 ± 0.07	1.03SerHis: 1.03 ± 0.027	5.221SerIle: 5.221 ± 0.085	4.593SerLys: 4.593 ± 0.076	6.185SerLeu: 6.185 ± 0.092	1.224SerMet: 1.224 ± 0.039	3.614SerAsn: 3.614 ± 0.072	2.051SerPro: 2.051 ± 0.051	2.181SerGln: 2.181 ± 0.048	2.022SerArg: 2.022 ± 0.042	4.074SerSer: 4.074 ± 0.075	3.561SerThr: 3.561 ± 0.07	3.922SerVal: 3.922 ± 0.063	0.628SerTrp: 0.628 ± 0.027	2.693SerTyr: 2.693 ± 0.059	0.0SerXaa: 0.0 ± 0.0
Thr	4.38ThrAla: 4.38 ± 0.092	0.41ThrCys: 0.41 ± 0.026	3.605ThrAsp: 3.605 ± 0.1	3.985ThrGlu: 3.985 ± 0.062	3.175ThrPhe: 3.175 ± 0.064	4.347ThrGly: 4.347 ± 0.123	1.039ThrHis: 1.039 ± 0.037	5.192ThrIle: 5.192 ± 0.089	3.966ThrLys: 3.966 ± 0.08	5.996ThrLeu: 5.996 ± 0.089	1.079ThrMet: 1.079 ± 0.036	3.404ThrAsn: 3.404 ± 0.076	2.631ThrPro: 2.631 ± 0.055	2.0ThrGln: 2.0 ± 0.05	1.836ThrArg: 1.836 ± 0.05	4.094ThrSer: 4.094 ± 0.075	4.083ThrThr: 4.083 ± 0.097	4.274ThrVal: 4.274 ± 0.093	0.568ThrTrp: 0.568 ± 0.027	2.483ThrTyr: 2.483 ± 0.058	0.0ThrXaa: 0.0 ± 0.0
Val	4.721ValAla: 4.721 ± 0.074	0.536ValCys: 0.536 ± 0.024	3.729ValAsp: 3.729 ± 0.082	3.838ValGlu: 3.838 ± 0.069	3.457ValPhe: 3.457 ± 0.069	3.857ValGly: 3.857 ± 0.07	0.986ValHis: 0.986 ± 0.036	5.182ValIle: 5.182 ± 0.078	4.366ValLys: 4.366 ± 0.079	6.016ValLeu: 6.016 ± 0.092	1.325ValMet: 1.325 ± 0.042	3.57ValAsn: 3.57 ± 0.072	2.133ValPro: 2.133 ± 0.052	1.883ValGln: 1.883 ± 0.044	2.001ValArg: 2.001 ± 0.051	4.679ValSer: 4.679 ± 0.075	4.244ValThr: 4.244 ± 0.106	4.612ValVal: 4.612 ± 0.081	0.608ValTrp: 0.608 ± 0.027	2.527ValTyr: 2.527 ± 0.048	0.0ValXaa: 0.0 ± 0.0
Trp	0.563TrpAla: 0.563 ± 0.028	0.097TrpCys: 0.097 ± 0.01	0.622TrpAsp: 0.622 ± 0.024	0.638TrpGlu: 0.638 ± 0.023	0.571TrpPhe: 0.571 ± 0.027	0.557TrpGly: 0.557 ± 0.028	0.203TrpHis: 0.203 ± 0.015	0.709TrpIle: 0.709 ± 0.032	0.681TrpLys: 0.681 ± 0.028	0.995TrpLeu: 0.995 ± 0.036	0.276TrpMet: 0.276 ± 0.02	0.607TrpAsn: 0.607 ± 0.027	0.229TrpPro: 0.229 ± 0.016	0.365TrpGln: 0.365 ± 0.02	0.379TrpArg: 0.379 ± 0.019	0.582TrpSer: 0.582 ± 0.027	0.509TrpThr: 0.509 ± 0.022	0.579TrpVal: 0.579 ± 0.023	0.145TrpTrp: 0.145 ± 0.014	0.425TrpTyr: 0.425 ± 0.024	0.0TrpXaa: 0.0 ± 0.0
Tyr	2.436TyrAla: 2.436 ± 0.054	0.33TyrCys: 0.33 ± 0.018	2.381TyrAsp: 2.381 ± 0.049	2.312TyrGlu: 2.312 ± 0.057	2.406TyrPhe: 2.406 ± 0.058	2.57TyrGly: 2.57 ± 0.054	0.795TyrHis: 0.795 ± 0.03	2.639TyrIle: 2.639 ± 0.056	2.99TyrLys: 2.99 ± 0.062	3.726TyrLeu: 3.726 ± 0.078	0.684TyrMet: 0.684 ± 0.026	2.535TyrAsn: 2.535 ± 0.057	1.386TyrPro: 1.386 ± 0.044	1.426TyrGln: 1.426 ± 0.04	1.515TyrArg: 1.515 ± 0.041	2.565TyrSer: 2.565 ± 0.06	2.518TyrThr: 2.518 ± 0.056	2.19TyrVal: 2.19 ± 0.049	0.432TyrTrp: 0.432 ± 0.023	1.83TyrTyr: 1.83 ± 0.057	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.0XaaAla: 0.0 ± 0.0	0.0XaaCys: 0.0 ± 0.0	0.0XaaAsp: 0.0 ± 0.0	0.0XaaGlu: 0.0 ± 0.0	0.0XaaPhe: 0.0 ± 0.0	0.0XaaGly: 0.0 ± 0.0	0.0XaaHis: 0.0 ± 0.0	0.0XaaIle: 0.0 ± 0.0	0.0XaaLys: 0.0 ± 0.0	0.0XaaLeu: 0.0 ± 0.0	0.0XaaMet: 0.0 ± 0.0	0.0XaaAsn: 0.0 ± 0.0	0.0XaaPro: 0.0 ± 0.0	0.0XaaGln: 0.0 ± 0.0	0.0XaaArg: 0.0 ± 0.0	0.0XaaSer: 0.0 ± 0.0	0.0XaaThr: 0.0 ± 0.0	0.0XaaVal: 0.0 ± 0.0	0.0XaaTrp: 0.0 ± 0.0	0.0XaaTyr: 0.0 ± 0.0	0.0XaaXaa: 0.0 ± 0.0

Statistics based on 2926 proteins (950377 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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