Aquaspirillum sp. LM1
Average proteome isoelectric point is 6.63
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 3268 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A1U9JLK1|A0A1U9JLK1_9NEIS Uncharacterized protein OS=Aquaspirillum sp. LM1 OX=1938604 GN=BXU06_07570 PE=4 SV=1
MM1 pKa = 7.89 DD2 pKa = 4.9 KK3 pKa = 10.82 SYY5 pKa = 10.0 TCILIADD12 pKa = 4.35 ATLQDD17 pKa = 4.69 LPVLLADD24 pKa = 4.9 LPASVHH30 pKa = 5.62 LRR32 pKa = 11.84 LVHH35 pKa = 6.23 SASEE39 pKa = 3.92 ALGEE43 pKa = 4.26 FWQALASAGLTQLHH57 pKa = 6.25 WLSHH61 pKa = 4.51 GMPGGVMIAGQALTAADD78 pKa = 4.42 FCQRR82 pKa = 11.84 FDD84 pKa = 3.47 GAAEE88 pKa = 4.06 RR89 pKa = 11.84 DD90 pKa = 3.42 LDD92 pKa = 3.85 VAFWSCHH99 pKa = 4.97 TGAGEE104 pKa = 3.6 PGYY107 pKa = 11.16 AFVEE111 pKa = 4.18 ALTRR115 pKa = 11.84 ATGAQVAASAVEE127 pKa = 4.15 VGAGFWQLSPAVNAPFSARR146 pKa = 11.84 AQLEE150 pKa = 4.28 FAHH153 pKa = 6.24 TLPAPAQFASSVTYY167 pKa = 10.17 QASQEE172 pKa = 4.11 MGGLALGDD180 pKa = 3.95 LNGDD184 pKa = 3.59 GTLDD188 pKa = 3.29 IQTTGIFNDD197 pKa = 3.55 ASTLINDD204 pKa = 4.07 GDD206 pKa = 3.93 GSFANDD212 pKa = 3.51 ASGTGQPNGEE222 pKa = 4.41 WGCEE226 pKa = 3.65 LADD229 pKa = 4.58 LNGDD233 pKa = 3.53 GHH235 pKa = 7.77 LDD237 pKa = 3.55 LVSRR241 pKa = 11.84 IYY243 pKa = 10.91 NAVVVNRR250 pKa = 11.84 GSAQNGFTNQQYY262 pKa = 9.78 YY263 pKa = 9.46 LQNEE267 pKa = 4.36 TVYY270 pKa = 10.94 DD271 pKa = 4.89 AIAAQVNNDD280 pKa = 2.78 GRR282 pKa = 11.84 FDD284 pKa = 4.38 LLVSRR289 pKa = 11.84 TIQGQQTLSVLLGQSDD305 pKa = 4.12 GTLAAPVNYY314 pKa = 10.24 ALASEE319 pKa = 4.67 GDD321 pKa = 3.83 ANQLHH326 pKa = 5.95 ATDD329 pKa = 5.09 LNNDD333 pKa = 3.62 GKK335 pKa = 10.9 QDD337 pKa = 4.13 VIALSYY343 pKa = 11.37 NNTISVLMNNGDD355 pKa = 3.68 GTFANSVSYY364 pKa = 10.86 QEE366 pKa = 5.42 GDD368 pKa = 3.58 YY369 pKa = 11.12 QNQLFNLTSADD380 pKa = 3.76 VNGDD384 pKa = 3.7 GYY386 pKa = 11.33 QDD388 pKa = 3.53 VVAVDD393 pKa = 3.47 IFAGSVSVKK402 pKa = 10.32 LNQGNGLLGPASHH415 pKa = 6.83 YY416 pKa = 9.74 ATGTAPTDD424 pKa = 3.36 IVAVDD429 pKa = 4.15 LNGDD433 pKa = 3.84 GAVDD437 pKa = 4.22 LATANTSSVSVLLGNGDD454 pKa = 3.45 GSFAAKK460 pKa = 9.89 QDD462 pKa = 3.45 IQLDD466 pKa = 4.02 TLPKK470 pKa = 9.91 HH471 pKa = 6.26 IRR473 pKa = 11.84 AGDD476 pKa = 3.66 LNQDD480 pKa = 3.62 GKK482 pKa = 11.14 QDD484 pKa = 3.95 LVVGSYY490 pKa = 10.26 SGNSVSVLLNTTGSVDD506 pKa = 3.65 SIAPVVSRR514 pKa = 11.84 LEE516 pKa = 3.94 VAGTSLIVHH525 pKa = 5.36 YY526 pKa = 8.96 TEE528 pKa = 3.94 ITQLDD533 pKa = 4.26 AVHH536 pKa = 6.95 PPALSQFAAKK546 pKa = 10.11 INNSPVALTGAPVVDD561 pKa = 4.52 AVHH564 pKa = 5.88 HH565 pKa = 5.74 TVTLTLGTPVALGQTVSLSYY585 pKa = 11.04 TDD587 pKa = 4.8 ASTNNDD593 pKa = 2.93 VQAVQDD599 pKa = 3.98 LAGNDD604 pKa = 3.43 AASFTLTTAPMAAVRR619 pKa = 11.84 FSADD623 pKa = 2.98 TGVSDD628 pKa = 4.41 SDD630 pKa = 5.29 LITFQPVQTLSGHH643 pKa = 5.97 LQTPTVDD650 pKa = 3.37 GQTVQVSVNNGMSWEE665 pKa = 4.3 TANHH669 pKa = 5.72 STGQTYY675 pKa = 9.06 WSLDD679 pKa = 3.34 QSLLGQSLYY688 pKa = 10.61 QGPNVLKK695 pKa = 10.88 VRR697 pKa = 11.84 LHH699 pKa = 5.48 TASSNTEE706 pKa = 3.98 VASQNYY712 pKa = 9.52 DD713 pKa = 3.57 LTWSGRR719 pKa = 11.84 IFMEE723 pKa = 4.25 SGSVNGNNIKK733 pKa = 10.24 VQFSSDD739 pKa = 3.52 LPLDD743 pKa = 4.05 ASHH746 pKa = 7.36 PPSLDD751 pKa = 3.34 SFEE754 pKa = 4.67 VLADD758 pKa = 3.73 NVAVALSGVAVVDD771 pKa = 4.4 GNARR775 pKa = 11.84 TLTLTLANPVLAGQQVLVKK794 pKa = 10.52 YY795 pKa = 9.71 NDD797 pKa = 3.45 PMPDD801 pKa = 3.47 DD802 pKa = 4.15 GQFILDD808 pKa = 4.92 AMDD811 pKa = 4.5 ALQDD815 pKa = 3.46 IGGADD820 pKa = 3.75 AMDD823 pKa = 3.68 GMAMLSNYY831 pKa = 9.05 TPDD834 pKa = 2.32 ITAPVFRR841 pKa = 11.84 SASINGSKK849 pKa = 10.49 LVLSYY854 pKa = 11.7 ADD856 pKa = 3.95 ASGLDD861 pKa = 3.98 VNNSPLLSAFSVKK874 pKa = 9.9 VDD876 pKa = 3.51 NSVVALTGTPVVNSGGKK893 pKa = 8.43 TITLTLVNSVLNGQAVTVSYY913 pKa = 10.84 TDD915 pKa = 3.44 PTSGNDD921 pKa = 3.02 SKK923 pKa = 11.41 AIQDD927 pKa = 3.68 VYY929 pKa = 11.69 GNDD932 pKa = 3.65 AASLAQVTVNNLTPIDD948 pKa = 3.69 ITPPVLTSATINGKK962 pKa = 9.59 KK963 pKa = 10.06 LVLSYY968 pKa = 11.65 ADD970 pKa = 3.82 ASALHH975 pKa = 5.77 SQIPLLSAFSVNVAGNAVLLSGGVPEE1001 pKa = 4.84 VDD1003 pKa = 3.25 TAAKK1007 pKa = 9.28 TVTLTLINPVLNGQVVTVSYY1027 pKa = 10.81 TDD1029 pKa = 3.48 PTSGNDD1035 pKa = 3.1 SQAIQDD1041 pKa = 3.76 VYY1043 pKa = 11.71 GNDD1046 pKa = 3.39 AASLNNVAVSNITPVDD1062 pKa = 3.43 TTPPVLTSATVSGHH1076 pKa = 5.21 EE1077 pKa = 4.31 VVLSYY1082 pKa = 11.34 TDD1084 pKa = 3.63 TNVLSSQGPLLSAFSVKK1101 pKa = 10.34 LAGSVATLSGAPVVNTTAKK1120 pKa = 10.19 TITLTLATPAQNGQAVTLSYY1140 pKa = 11.02 TDD1142 pKa = 3.56 PTSGNDD1148 pKa = 3.14 SQAIQDD1154 pKa = 3.63 ASGNDD1159 pKa = 3.35 AASLNNIAVSNLTPLGDD1176 pKa = 3.48 VTPPVLTSATVNGDD1190 pKa = 3.5 QVLLSYY1196 pKa = 10.56 TDD1198 pKa = 3.66 SSPLKK1203 pKa = 10.27 SSMGGLYY1210 pKa = 9.95 VIKK1213 pKa = 10.54 VDD1215 pKa = 3.6 GVVAQTYY1222 pKa = 10.87 GMTANPSNNTILITLANAVLAGQVVTFSYY1251 pKa = 10.98 TDD1253 pKa = 3.46 PTSGNDD1259 pKa = 3.35 LLAIQDD1265 pKa = 3.69 VAGNDD1270 pKa = 3.42 AASVVDD1276 pKa = 5.07 FSVSNLTVPTYY1287 pKa = 11.29 QMAAGEE1293 pKa = 4.13 TSYY1296 pKa = 10.71 TVTATQYY1303 pKa = 11.43 NVMGNALDD1311 pKa = 3.86 NLMTGDD1317 pKa = 4.02 SKK1319 pKa = 11.52 PNNLMGGAGNDD1330 pKa = 3.56 TLLGGGNNGDD1340 pKa = 3.59 RR1341 pKa = 11.84 LYY1343 pKa = 11.44 GEE1345 pKa = 5.05 AGNDD1349 pKa = 3.52 VLNSGLGGSHH1359 pKa = 6.73 RR1360 pKa = 11.84 LVGGSGDD1367 pKa = 3.66 DD1368 pKa = 3.33 RR1369 pKa = 11.84 YY1370 pKa = 10.92 EE1371 pKa = 3.74 IAYY1374 pKa = 9.78 LATLLVEE1381 pKa = 5.17 QPDD1384 pKa = 3.75 EE1385 pKa = 4.95 GNDD1388 pKa = 3.39 SVYY1391 pKa = 11.33 VMPGANLVVSLITLSANIEE1410 pKa = 3.86 NLIAKK1415 pKa = 9.86 NSEE1418 pKa = 3.89 SFNVVGNASDD1428 pKa = 4.04 NYY1430 pKa = 11.17 LLGNQASGLLKK1441 pKa = 10.68 GLAGNDD1447 pKa = 3.52 TLISQGGDD1455 pKa = 3.19 DD1456 pKa = 4.1 TLEE1459 pKa = 4.65 GGDD1462 pKa = 4.22 GDD1464 pKa = 5.64 DD1465 pKa = 4.12 VIAPVAHH1472 pKa = 6.13 SHH1474 pKa = 4.35 MTEE1477 pKa = 3.65 NTRR1480 pKa = 11.84 VLTGNMQLSGGNGADD1495 pKa = 2.76 IFVMAEE1501 pKa = 4.08 GFVGDD1506 pKa = 5.78 HH1507 pKa = 6.62 KK1508 pKa = 11.35 DD1509 pKa = 3.0 GWNPNEE1515 pKa = 3.99 FSGNYY1520 pKa = 8.45 YY1521 pKa = 10.66 SPSQPVGIQGVYY1533 pKa = 10.02 ISDD1536 pKa = 4.07 FEE1538 pKa = 4.76 RR1539 pKa = 11.84 GVDD1542 pKa = 3.98 KK1543 pKa = 11.01 ISLTLSEE1550 pKa = 4.55 AALVPTTVNTLTLTAGMTLASAVDD1574 pKa = 3.75 QAASRR1579 pKa = 11.84 GSAAAPQVTIILNEE1593 pKa = 4.13 NVGCAYY1599 pKa = 10.7 LVLDD1603 pKa = 4.33 QSDD1606 pKa = 3.61 ASTFSSNDD1614 pKa = 3.09 LAIKK1618 pKa = 10.38 LNYY1621 pKa = 10.27 SMPLGIGDD1629 pKa = 4.72 LMFTKK1634 pKa = 10.78 LSQQNQLLPAEE1645 pKa = 4.33 SAPPVFTQASVNGNQLVLSYY1665 pKa = 11.3 SDD1667 pKa = 4.79 ASPLDD1672 pKa = 4.04 SVALPLGAFAVMVNGNAASVSAASVNSLARR1702 pKa = 11.84 TVTLTLASPVQNGQAVTVSYY1722 pKa = 10.58 TDD1724 pKa = 3.62 PSSGNDD1730 pKa = 2.9 SAAIQDD1736 pKa = 3.93 RR1737 pKa = 11.84 YY1738 pKa = 11.38 GNDD1741 pKa = 3.34 ATSLAATPVNNLTTPADD1758 pKa = 3.46 VTPPVLQSASLTDD1771 pKa = 3.43 STLVLTYY1778 pKa = 10.86 SDD1780 pKa = 4.25 TSPLDD1785 pKa = 3.64 AAHH1788 pKa = 7.11 PPALSAFNVKK1798 pKa = 9.58 VAGVTVSLTGTPVVNATARR1817 pKa = 11.84 TVTLTLADD1825 pKa = 3.88 PVLAGQAVTVSYY1837 pKa = 10.58 TDD1839 pKa = 3.64 PSSGNDD1845 pKa = 3.2 AQAVQDD1851 pKa = 3.95 GSGNDD1856 pKa = 3.33 AQTVLNRR1863 pKa = 11.84 VVSNLGTNTSYY1874 pKa = 11.92 VMDD1877 pKa = 4.22 ATTVNYY1883 pKa = 7.68 TATGTAALTIQGNALNNQITGNAADD1908 pKa = 3.63 NVIRR1912 pKa = 11.84 GGQGNDD1918 pKa = 2.95 RR1919 pKa = 11.84 LLGGLGNDD1927 pKa = 3.4 QLLGEE1932 pKa = 5.36 DD1933 pKa = 4.88 GSDD1936 pKa = 3.82 SLDD1939 pKa = 3.51 GGAGSDD1945 pKa = 3.84 TLSGGAGNDD1954 pKa = 3.14 AYY1956 pKa = 11.36 YY1957 pKa = 10.84 VDD1959 pKa = 5.13 SVDD1962 pKa = 4.55 DD1963 pKa = 4.34 QIIEE1967 pKa = 4.03 QANDD1971 pKa = 3.54 SIADD1975 pKa = 3.68 TVYY1978 pKa = 11.43 ASINYY1983 pKa = 8.31 TLGNDD1988 pKa = 3.79 LEE1990 pKa = 4.68 HH1991 pKa = 6.64 LVLMGQATQGQGNEE2005 pKa = 4.16 LANGLTGNALANTLTGLAGDD2025 pKa = 4.51 DD2026 pKa = 4.8 RR2027 pKa = 11.84 IDD2029 pKa = 3.71 GKK2031 pKa = 10.93 QGADD2035 pKa = 3.02 TMLGGTGNDD2044 pKa = 3.52 YY2045 pKa = 11.24 YY2046 pKa = 11.61 YY2047 pKa = 11.12 VDD2049 pKa = 3.48 QAGDD2053 pKa = 3.89 SVIEE2057 pKa = 4.2 LANEE2061 pKa = 4.25 GNDD2064 pKa = 3.59 TIYY2067 pKa = 10.86 AYY2069 pKa = 11.09 LDD2071 pKa = 3.42 YY2072 pKa = 11.03 TLGANQEE2079 pKa = 4.18 GLNLLGTLAVVGQGNEE2095 pKa = 4.18 LGNMLNGNALDD2106 pKa = 3.82 NTLMGLAGNDD2116 pKa = 3.44 RR2117 pKa = 11.84 LDD2119 pKa = 3.97 GKK2121 pKa = 10.7 QGADD2125 pKa = 3.39 TLIGGLGNDD2134 pKa = 3.81 TYY2136 pKa = 11.54 YY2137 pKa = 11.13 VDD2139 pKa = 3.45 HH2140 pKa = 6.76 TADD2143 pKa = 3.25 VVTEE2147 pKa = 3.96 QANEE2151 pKa = 4.25 GVDD2154 pKa = 3.42 TVYY2157 pKa = 11.4 ASVSHH2162 pKa = 6.25 TLADD2166 pKa = 3.56 NVEE2169 pKa = 4.46 TLRR2172 pKa = 11.84 MTADD2176 pKa = 3.27 GQTAIGNAANNVIYY2190 pKa = 10.42 GSAGQDD2196 pKa = 3.32 VLIDD2200 pKa = 3.81 GGEE2203 pKa = 4.26 GNDD2206 pKa = 3.89 TIYY2209 pKa = 10.95 TLGDD2213 pKa = 3.11 KK2214 pKa = 10.95 GGEE2217 pKa = 3.98 QHH2219 pKa = 5.7 VHH2221 pKa = 6.25 GGLGSDD2227 pKa = 3.5 TLYY2230 pKa = 11.4 VSGTLANGDD2239 pKa = 3.61 RR2240 pKa = 11.84 GSYY2243 pKa = 10.51 VYY2245 pKa = 11.14 GDD2247 pKa = 4.41 AGDD2250 pKa = 4.08 DD2251 pKa = 3.79 TLNATQGDD2259 pKa = 4.27 VKK2261 pKa = 10.94 SDD2263 pKa = 3.54 LMGGAGNDD2271 pKa = 3.23 TYY2273 pKa = 11.52 VINNIHH2279 pKa = 6.04 NRR2281 pKa = 11.84 VIEE2284 pKa = 4.09 NVNDD2288 pKa = 4.83 GYY2290 pKa = 10.75 DD2291 pKa = 3.43 TVQTSVSFSLQAGEE2305 pKa = 4.18 MFIPDD2310 pKa = 3.88 SVDD2313 pKa = 3.4 VPVTTGNVEE2322 pKa = 4.07 RR2323 pKa = 11.84 LLMTGTANIDD2333 pKa = 3.34 GFGNEE2338 pKa = 4.27 LVNYY2342 pKa = 10.33 LLGNAGNNVLLGRR2355 pKa = 11.84 DD2356 pKa = 3.63 GADD2359 pKa = 3.04 TLVGQGGNDD2368 pKa = 3.82 LLDD2371 pKa = 4.16 GGNGNDD2377 pKa = 3.25 TLAPVTHH2384 pKa = 7.0 ASSLGTPNVHH2394 pKa = 6.56 TGQITLTGGNGSDD2407 pKa = 4.2 VFWMTQGYY2415 pKa = 9.88 VGDD2418 pKa = 4.45 HH2419 pKa = 6.27 SSGGNTLTLSDD2430 pKa = 4.6 FVHH2433 pKa = 6.57 GQDD2436 pKa = 3.31 KK2437 pKa = 11.4 VKK2439 pKa = 9.72 FTLSLSATLPTTLTTLTPSGGATLASLLNQAAVAGSASAPKK2480 pKa = 8.85 VTAFAFAGDD2489 pKa = 3.93 TYY2491 pKa = 11.47 LVLDD2495 pKa = 3.76 QTSASSFTSDD2505 pKa = 3.92 DD2506 pKa = 3.61 LAIKK2510 pKa = 10.73 LSGTPAFTFSDD2521 pKa = 3.73 LAFVKK2526 pKa = 9.84 MM2527 pKa = 4.41
Molecular weight: 259.32 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.673
IPC2_protein 3.757
IPC_protein 3.821
Toseland 3.579
ProMoST 3.999
Dawson 3.834
Bjellqvist 3.986
Wikipedia 3.795
Rodwell 3.643
Grimsley 3.478
Solomon 3.834
Lehninger 3.795
Nozaki 3.935
DTASelect 4.253
Thurlkill 3.643
EMBOSS 3.808
Sillero 3.948
Patrickios 1.176
IPC_peptide 3.821
IPC2_peptide 3.923
IPC2.peptide.svr19 3.828
Protein with the highest isoelectric point:
>tr|A0A1U9JK21|A0A1U9JK21_9NEIS Lon protease OS=Aquaspirillum sp. LM1 OX=1938604 GN=lon PE=2 SV=1
MM1 pKa = 7.45 KK2 pKa = 10.34 KK3 pKa = 10.09 SALHH7 pKa = 6.09 AGKK10 pKa = 8.35 TGKK13 pKa = 7.04 TQRR16 pKa = 11.84 SRR18 pKa = 11.84 LARR21 pKa = 11.84 PTAKK25 pKa = 9.28 ATKK28 pKa = 8.76 PLKK31 pKa = 10.38 KK32 pKa = 9.81 SVRR35 pKa = 11.84 HH36 pKa = 5.21 PQVKK40 pKa = 9.8 SSKK43 pKa = 9.01 PRR45 pKa = 11.84 QGKK48 pKa = 10.33 GNVAKK53 pKa = 9.66 TKK55 pKa = 10.69 ASTRR59 pKa = 11.84 QHH61 pKa = 4.9 TQRR64 pKa = 11.84 QRR66 pKa = 11.84 KK67 pKa = 7.63 RR68 pKa = 11.84 QQQ70 pKa = 2.66
Molecular weight: 7.88 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.503
IPC2_protein 11.213
IPC_protein 12.749
Toseland 12.925
ProMoST 13.408
Dawson 12.925
Bjellqvist 12.91
Wikipedia 13.393
Rodwell 12.837
Grimsley 12.969
Solomon 13.408
Lehninger 13.32
Nozaki 12.925
DTASelect 12.91
Thurlkill 12.925
EMBOSS 13.422
Sillero 12.925
Patrickios 12.559
IPC_peptide 13.422
IPC2_peptide 12.398
IPC2.peptide.svr19 9.091
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
3268
0
3268
1103201
26
3224
337.6
36.81
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
12.418 ± 0.063
1.058 ± 0.017
5.467 ± 0.033
5.086 ± 0.039
3.323 ± 0.023
7.747 ± 0.047
2.578 ± 0.021
4.313 ± 0.031
3.144 ± 0.036
11.643 ± 0.059
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.323 ± 0.021
2.847 ± 0.035
5.063 ± 0.044
4.813 ± 0.043
6.511 ± 0.051
5.628 ± 0.039
5.079 ± 0.054
7.071 ± 0.039
1.511 ± 0.021
2.376 ± 0.025
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here