Amino acid dipepetide frequency for Aquaspirillum sp. LM1

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
15.684AlaAla: 15.684 ± 0.184
1.522AlaCys: 1.522 ± 0.036
7.327AlaAsp: 7.327 ± 0.107
7.293AlaGlu: 7.293 ± 0.098
3.532AlaPhe: 3.532 ± 0.058
9.979AlaGly: 9.979 ± 0.127
2.912AlaHis: 2.912 ± 0.063
5.024AlaIle: 5.024 ± 0.073
3.39AlaLys: 3.39 ± 0.069
15.455AlaLeu: 15.455 ± 0.163
3.263AlaMet: 3.263 ± 0.066
3.183AlaAsn: 3.183 ± 0.077
5.243AlaPro: 5.243 ± 0.102
6.173AlaGln: 6.173 ± 0.082
8.691AlaArg: 8.691 ± 0.105
7.573AlaSer: 7.573 ± 0.103
5.405AlaThr: 5.405 ± 0.091
8.11AlaVal: 8.11 ± 0.094
1.874AlaTrp: 1.874 ± 0.053
2.55AlaTyr: 2.55 ± 0.055
0.0AlaXaa: 0.0 ± 0.0
Cys
1.282CysAla: 1.282 ± 0.036
0.197CysCys: 0.197 ± 0.014
0.507CysAsp: 0.507 ± 0.021
0.48CysGlu: 0.48 ± 0.02
0.361CysPhe: 0.361 ± 0.018
0.999CysGly: 0.999 ± 0.032
0.358CysHis: 0.358 ± 0.018
0.394CysIle: 0.394 ± 0.018
0.269CysLys: 0.269 ± 0.014
1.112CysLeu: 1.112 ± 0.03
0.204CysMet: 0.204 ± 0.014
0.259CysAsn: 0.259 ± 0.018
0.661CysPro: 0.661 ± 0.029
0.526CysGln: 0.526 ± 0.026
0.739CysArg: 0.739 ± 0.024
0.532CysSer: 0.532 ± 0.023
0.48CysThr: 0.48 ± 0.018
0.732CysVal: 0.732 ± 0.027
0.18CysTrp: 0.18 ± 0.015
0.305CysTyr: 0.305 ± 0.018
0.0CysXaa: 0.0 ± 0.0
Asp
7.305AspAla: 7.305 ± 0.101
0.581AspCys: 0.581 ± 0.025
2.955AspAsp: 2.955 ± 0.066
2.988AspGlu: 2.988 ± 0.059
1.909AspPhe: 1.909 ± 0.04
4.512AspGly: 4.512 ± 0.073
1.17AspHis: 1.17 ± 0.036
2.852AspIle: 2.852 ± 0.056
1.807AspLys: 1.807 ± 0.043
5.129AspLeu: 5.129 ± 0.084
1.312AspMet: 1.312 ± 0.039
1.592AspAsn: 1.592 ± 0.04
2.843AspPro: 2.843 ± 0.053
2.042AspGln: 2.042 ± 0.044
2.828AspArg: 2.828 ± 0.053
3.126AspSer: 3.126 ± 0.058
3.236AspThr: 3.236 ± 0.066
3.619AspVal: 3.619 ± 0.067
1.206AspTrp: 1.206 ± 0.043
1.66AspTyr: 1.66 ± 0.049
0.0AspXaa: 0.0 ± 0.0
Glu
6.436GluAla: 6.436 ± 0.095
0.448GluCys: 0.448 ± 0.021
2.204GluAsp: 2.204 ± 0.05
2.201GluGlu: 2.201 ± 0.061
1.745GluPhe: 1.745 ± 0.035
3.208GluGly: 3.208 ± 0.059
1.558GluHis: 1.558 ± 0.042
2.561GluIle: 2.561 ± 0.061
1.846GluLys: 1.846 ± 0.054
6.023GluLeu: 6.023 ± 0.083
1.209GluMet: 1.209 ± 0.035
1.386GluAsn: 1.386 ± 0.041
2.132GluPro: 2.132 ± 0.057
3.319GluGln: 3.319 ± 0.063
4.077GluArg: 4.077 ± 0.073
2.195GluSer: 2.195 ± 0.056
2.514GluThr: 2.514 ± 0.057
4.009GluVal: 4.009 ± 0.06
0.666GluTrp: 0.666 ± 0.023
1.127GluTyr: 1.127 ± 0.035
0.0GluXaa: 0.0 ± 0.0
Phe
4.499PheAla: 4.499 ± 0.073
0.392PheCys: 0.392 ± 0.019
2.273PheAsp: 2.273 ± 0.05
1.631PheGlu: 1.631 ± 0.038
1.242PhePhe: 1.242 ± 0.039
2.78PheGly: 2.78 ± 0.058
0.703PheHis: 0.703 ± 0.027
1.593PheIle: 1.593 ± 0.045
1.053PheLys: 1.053 ± 0.032
2.88PheLeu: 2.88 ± 0.065
0.715PheMet: 0.715 ± 0.025
1.187PheAsn: 1.187 ± 0.031
1.371PhePro: 1.371 ± 0.031
1.08PheGln: 1.08 ± 0.034
1.806PheArg: 1.806 ± 0.046
2.225PheSer: 2.225 ± 0.048
1.867PheThr: 1.867 ± 0.048
2.453PheVal: 2.453 ± 0.056
0.499PheTrp: 0.499 ± 0.023
0.984PheTyr: 0.984 ± 0.031
0.0PheXaa: 0.0 ± 0.0
Gly
7.162GlyAla: 7.162 ± 0.105
0.98GlyCys: 0.98 ± 0.032
3.881GlyAsp: 3.881 ± 0.073
4.757GlyGlu: 4.757 ± 0.074
3.088GlyPhe: 3.088 ± 0.055
5.999GlyGly: 5.999 ± 0.111
2.09GlyHis: 2.09 ± 0.045
3.589GlyIle: 3.589 ± 0.072
3.366GlyLys: 3.366 ± 0.061
8.648GlyLeu: 8.648 ± 0.096
2.413GlyMet: 2.413 ± 0.051
2.47GlyAsn: 2.47 ± 0.112
2.389GlyPro: 2.389 ± 0.051
4.413GlyGln: 4.413 ± 0.062
4.675GlyArg: 4.675 ± 0.07
4.307GlySer: 4.307 ± 0.079
3.22GlyThr: 3.22 ± 0.081
6.293GlyVal: 6.293 ± 0.084
1.488GlyTrp: 1.488 ± 0.041
2.243GlyTyr: 2.243 ± 0.045
0.0GlyXaa: 0.0 ± 0.0
His
3.373HisAla: 3.373 ± 0.063
0.353HisCys: 0.353 ± 0.019
1.428HisAsp: 1.428 ± 0.039
1.035HisGlu: 1.035 ± 0.031
0.888HisPhe: 0.888 ± 0.03
2.31HisGly: 2.31 ± 0.045
0.788HisHis: 0.788 ± 0.028
1.059HisIle: 1.059 ± 0.031
0.664HisLys: 0.664 ± 0.027
2.521HisLeu: 2.521 ± 0.057
0.494HisMet: 0.494 ± 0.022
0.605HisAsn: 0.605 ± 0.025
1.89HisPro: 1.89 ± 0.043
1.175HisGln: 1.175 ± 0.034
1.442HisArg: 1.442 ± 0.04
1.403HisSer: 1.403 ± 0.037
1.57HisThr: 1.57 ± 0.042
1.395HisVal: 1.395 ± 0.038
0.586HisTrp: 0.586 ± 0.029
0.805HisTyr: 0.805 ± 0.028
0.0HisXaa: 0.0 ± 0.0
Ile
5.499IleAla: 5.499 ± 0.088
0.408IleCys: 0.408 ± 0.018
3.158IleAsp: 3.158 ± 0.06
2.864IleGlu: 2.864 ± 0.059
1.155IlePhe: 1.155 ± 0.03
4.066IleGly: 4.066 ± 0.074
1.07IleHis: 1.07 ± 0.03
1.651IleIle: 1.651 ± 0.047
1.471IleLys: 1.471 ± 0.042
3.687IleLeu: 3.687 ± 0.073
0.727IleMet: 0.727 ± 0.028
1.544IleAsn: 1.544 ± 0.041
2.068IlePro: 2.068 ± 0.053
1.598IleGln: 1.598 ± 0.046
2.786IleArg: 2.786 ± 0.055
2.591IleSer: 2.591 ± 0.043
2.357IleThr: 2.357 ± 0.051
2.953IleVal: 2.953 ± 0.058
0.461IleTrp: 0.461 ± 0.02
1.013IleTyr: 1.013 ± 0.03
0.0IleXaa: 0.0 ± 0.0
Lys
3.55LysAla: 3.55 ± 0.065
0.2LysCys: 0.2 ± 0.014
1.467LysAsp: 1.467 ± 0.042
1.336LysGlu: 1.336 ± 0.044
0.893LysPhe: 0.893 ± 0.03
2.113LysGly: 2.113 ± 0.053
0.718LysHis: 0.718 ± 0.028
1.487LysIle: 1.487 ± 0.041
1.493LysLys: 1.493 ± 0.05
3.83LysLeu: 3.83 ± 0.068
0.773LysMet: 0.773 ± 0.027
1.114LysAsn: 1.114 ± 0.034
1.953LysPro: 1.953 ± 0.061
1.528LysGln: 1.528 ± 0.042
1.926LysArg: 1.926 ± 0.039
1.609LysSer: 1.609 ± 0.045
1.989LysThr: 1.989 ± 0.047
2.405LysVal: 2.405 ± 0.053
0.374LysTrp: 0.374 ± 0.02
0.68LysTyr: 0.68 ± 0.029
0.0LysXaa: 0.0 ± 0.0
Leu
17.43LeuAla: 17.43 ± 0.193
1.229LeuCys: 1.229 ± 0.039
6.904LeuAsp: 6.904 ± 0.094
5.277LeuGlu: 5.277 ± 0.086
3.512LeuPhe: 3.512 ± 0.06
8.785LeuGly: 8.785 ± 0.11
2.757LeuHis: 2.757 ± 0.061
4.726LeuIle: 4.726 ± 0.072
3.767LeuLys: 3.767 ± 0.068
12.766LeuLeu: 12.766 ± 0.171
2.388LeuMet: 2.388 ± 0.055
3.629LeuAsn: 3.629 ± 0.061
6.784LeuPro: 6.784 ± 0.111
4.199LeuGln: 4.199 ± 0.066
7.296LeuArg: 7.296 ± 0.106
7.437LeuSer: 7.437 ± 0.097
6.662LeuThr: 6.662 ± 0.098
7.063LeuVal: 7.063 ± 0.106
1.429LeuTrp: 1.429 ± 0.048
2.39LeuTyr: 2.39 ± 0.057
0.0LeuXaa: 0.0 ± 0.0
Met
2.944MetAla: 2.944 ± 0.054
0.165MetCys: 0.165 ± 0.011
1.143MetAsp: 1.143 ± 0.028
0.962MetGlu: 0.962 ± 0.034
0.662MetPhe: 0.662 ± 0.024
1.652MetGly: 1.652 ± 0.043
0.634MetHis: 0.634 ± 0.027
0.967MetIle: 0.967 ± 0.03
0.843MetLys: 0.843 ± 0.028
2.718MetLeu: 2.718 ± 0.058
0.577MetMet: 0.577 ± 0.026
0.755MetAsn: 0.755 ± 0.024
1.323MetPro: 1.323 ± 0.036
1.128MetGln: 1.128 ± 0.034
1.501MetArg: 1.501 ± 0.039
1.536MetSer: 1.536 ± 0.042
1.468MetThr: 1.468 ± 0.037
1.708MetVal: 1.708 ± 0.044
0.182MetTrp: 0.182 ± 0.014
0.363MetTyr: 0.363 ± 0.018
0.0MetXaa: 0.0 ± 0.0
Asn
3.271AsnAla: 3.271 ± 0.066
0.255AsnCys: 0.255 ± 0.015
1.625AsnAsp: 1.625 ± 0.064
1.147AsnGlu: 1.147 ± 0.036
0.867AsnPhe: 0.867 ± 0.034
2.48AsnGly: 2.48 ± 0.068
0.72AsnHis: 0.72 ± 0.023
1.371AsnIle: 1.371 ± 0.041
0.867AsnLys: 0.867 ± 0.025
3.148AsnLeu: 3.148 ± 0.074
0.626AsnMet: 0.626 ± 0.024
0.93AsnAsn: 0.93 ± 0.047
1.839AsnPro: 1.839 ± 0.039
1.348AsnGln: 1.348 ± 0.038
1.795AsnArg: 1.795 ± 0.04
1.512AsnSer: 1.512 ± 0.051
1.714AsnThr: 1.714 ± 0.061
1.829AsnVal: 1.829 ± 0.048
0.423AsnTrp: 0.423 ± 0.02
0.704AsnTyr: 0.704 ± 0.027
0.0AsnXaa: 0.0 ± 0.0
Pro
7.282ProAla: 7.282 ± 0.133
0.46ProCys: 0.46 ± 0.019
3.586ProAsp: 3.586 ± 0.063
3.049ProGlu: 3.049 ± 0.067
1.645ProPhe: 1.645 ± 0.045
4.058ProGly: 4.058 ± 0.061
1.168ProHis: 1.168 ± 0.037
1.865ProIle: 1.865 ± 0.042
1.392ProLys: 1.392 ± 0.044
5.558ProLeu: 5.558 ± 0.097
1.14ProMet: 1.14 ± 0.033
1.221ProAsn: 1.221 ± 0.035
2.689ProPro: 2.689 ± 0.067
1.909ProGln: 1.909 ± 0.048
2.61ProArg: 2.61 ± 0.055
2.554ProSer: 2.554 ± 0.05
2.418ProThr: 2.418 ± 0.071
4.135ProVal: 4.135 ± 0.07
0.809ProTrp: 0.809 ± 0.031
1.084ProTyr: 1.084 ± 0.032
0.0ProXaa: 0.0 ± 0.0
Gln
7.134GlnAla: 7.134 ± 0.082
0.41GlnCys: 0.41 ± 0.018
1.873GlnAsp: 1.873 ± 0.039
1.789GlnGlu: 1.789 ± 0.042
1.42GlnPhe: 1.42 ± 0.038
3.495GlnGly: 3.495 ± 0.056
1.558GlnHis: 1.558 ± 0.042
2.06GlnIle: 2.06 ± 0.052
1.177GlnLys: 1.177 ± 0.037
6.074GlnLeu: 6.074 ± 0.097
1.012GlnMet: 1.012 ± 0.029
0.98GlnAsn: 0.98 ± 0.033
2.74GlnPro: 2.74 ± 0.058
3.019GlnGln: 3.019 ± 0.074
3.791GlnArg: 3.791 ± 0.075
2.137GlnSer: 2.137 ± 0.043
2.32GlnThr: 2.32 ± 0.053
3.52GlnVal: 3.52 ± 0.059
0.706GlnTrp: 0.706 ± 0.033
0.914GlnTyr: 0.914 ± 0.029
0.0GlnXaa: 0.0 ± 0.0
Arg
6.717ArgAla: 6.717 ± 0.092
0.647ArgCys: 0.647 ± 0.027
3.53ArgAsp: 3.53 ± 0.059
3.78ArgGlu: 3.78 ± 0.067
2.762ArgPhe: 2.762 ± 0.049
3.788ArgGly: 3.788 ± 0.061
2.233ArgHis: 2.233 ± 0.052
3.003ArgIle: 3.003 ± 0.063
1.838ArgLys: 1.838 ± 0.047
8.407ArgLeu: 8.407 ± 0.119
1.677ArgMet: 1.677 ± 0.038
1.652ArgAsn: 1.652 ± 0.042
2.982ArgPro: 2.982 ± 0.06
4.242ArgGln: 4.242 ± 0.078
4.549ArgArg: 4.549 ± 0.086
2.995ArgSer: 2.995 ± 0.053
2.657ArgThr: 2.657 ± 0.055
4.392ArgVal: 4.392 ± 0.073
1.227ArgTrp: 1.227 ± 0.04
2.028ArgTyr: 2.028 ± 0.044
0.0ArgXaa: 0.0 ± 0.0
Ser
6.836SerAla: 6.836 ± 0.094
0.567SerCys: 0.567 ± 0.026
2.71SerAsp: 2.71 ± 0.055
2.695SerGlu: 2.695 ± 0.057
1.904SerPhe: 1.904 ± 0.041
5.113SerGly: 5.113 ± 0.091
1.541SerHis: 1.541 ± 0.04
2.35SerIle: 2.35 ± 0.049
1.587SerLys: 1.587 ± 0.046
6.562SerLeu: 6.562 ± 0.091
1.274SerMet: 1.274 ± 0.032
1.503SerAsn: 1.503 ± 0.043
2.893SerPro: 2.893 ± 0.053
2.78SerGln: 2.78 ± 0.058
3.687SerArg: 3.687 ± 0.066
3.268SerSer: 3.268 ± 0.078
2.81SerThr: 2.81 ± 0.073
4.06SerVal: 4.06 ± 0.079
0.77SerTrp: 0.77 ± 0.029
1.37SerTyr: 1.37 ± 0.042
0.0SerXaa: 0.0 ± 0.0
Thr
5.389ThrAla: 5.389 ± 0.092
0.443ThrCys: 0.443 ± 0.021
2.58ThrAsp: 2.58 ± 0.063
2.264ThrGlu: 2.264 ± 0.044
1.613ThrPhe: 1.613 ± 0.041
4.385ThrGly: 4.385 ± 0.093
1.275ThrHis: 1.275 ± 0.037
1.971ThrIle: 1.971 ± 0.049
0.979ThrLys: 0.979 ± 0.035
8.013ThrLeu: 8.013 ± 0.116
0.815ThrMet: 0.815 ± 0.027
1.117ThrAsn: 1.117 ± 0.04
3.719ThrPro: 3.719 ± 0.077
2.292ThrGln: 2.292 ± 0.044
3.213ThrArg: 3.213 ± 0.062
2.547ThrSer: 2.547 ± 0.068
2.616ThrThr: 2.616 ± 0.074
3.971ThrVal: 3.971 ± 0.083
0.581ThrTrp: 0.581 ± 0.024
1.003ThrTyr: 1.003 ± 0.036
0.0ThrXaa: 0.0 ± 0.0
Val
8.353ValAla: 8.353 ± 0.104
0.859ValCys: 0.859 ± 0.029
3.735ValAsp: 3.735 ± 0.066
3.871ValGlu: 3.871 ± 0.072
2.566ValPhe: 2.566 ± 0.058
4.952ValGly: 4.952 ± 0.074
1.455ValHis: 1.455 ± 0.036
3.261ValIle: 3.261 ± 0.06
2.481ValLys: 2.481 ± 0.058
8.285ValLeu: 8.285 ± 0.114
1.803ValMet: 1.803 ± 0.04
2.324ValAsn: 2.324 ± 0.057
2.994ValPro: 2.994 ± 0.058
2.697ValGln: 2.697 ± 0.056
4.609ValArg: 4.609 ± 0.078
4.759ValSer: 4.759 ± 0.083
3.581ValThr: 3.581 ± 0.081
5.457ValVal: 5.457 ± 0.089
1.015ValTrp: 1.015 ± 0.032
1.653ValTyr: 1.653 ± 0.042
0.0ValXaa: 0.0 ± 0.0
Trp
1.391TrpAla: 1.391 ± 0.039
0.181TrpCys: 0.181 ± 0.015
0.577TrpAsp: 0.577 ± 0.026
0.52TrpGlu: 0.52 ± 0.022
0.521TrpPhe: 0.521 ± 0.024
0.872TrpGly: 0.872 ± 0.034
0.451TrpHis: 0.451 ± 0.022
0.528TrpIle: 0.528 ± 0.026
0.444TrpLys: 0.444 ± 0.019
2.754TrpLeu: 2.754 ± 0.075
0.421TrpMet: 0.421 ± 0.021
0.386TrpAsn: 0.386 ± 0.019
0.79TrpPro: 0.79 ± 0.031
1.204TrpGln: 1.204 ± 0.041
1.285TrpArg: 1.285 ± 0.039
0.681TrpSer: 0.681 ± 0.026
0.534TrpThr: 0.534 ± 0.021
1.058TrpVal: 1.058 ± 0.029
0.236TrpTrp: 0.236 ± 0.018
0.274TrpTyr: 0.274 ± 0.017
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.648TyrAla: 2.648 ± 0.062
0.279TyrCys: 0.279 ± 0.015
1.21TyrAsp: 1.21 ± 0.037
0.925TyrGlu: 0.925 ± 0.026
0.947TyrPhe: 0.947 ± 0.031
1.923TyrGly: 1.923 ± 0.05
0.625TyrHis: 0.625 ± 0.026
0.819TyrIle: 0.819 ± 0.028
0.703TyrLys: 0.703 ± 0.025
2.862TyrLeu: 2.862 ± 0.057
0.385TyrMet: 0.385 ± 0.019
0.635TyrAsn: 0.635 ± 0.028
1.331TyrPro: 1.331 ± 0.037
1.458TyrGln: 1.458 ± 0.036
1.798TyrArg: 1.798 ± 0.049
1.294TyrSer: 1.294 ± 0.036
1.368TyrThr: 1.368 ± 0.041
1.549TyrVal: 1.549 ± 0.037
0.392TyrTrp: 0.392 ± 0.02
0.615TyrTyr: 0.615 ± 0.028
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 3268 proteins (1103201 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski