Paenibacillus naphthalenovorans

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Bacilli; Bacillales; Paenibacillaceae; Paenibacillus

Average proteome isoelectric point is 6.53

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 5049 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0U2UH02|A0A0U2UH02_9BACL Uncharacterized protein OS=Paenibacillus naphthalenovorans OX=162209 GN=IJ22_50770 PE=4 SV=1
MM1 pKa = 7.58NFPTNRR7 pKa = 11.84NVTLLQTRR15 pKa = 11.84GVAAMEE21 pKa = 4.26LPEE24 pKa = 4.4VTTDD28 pKa = 3.18MLFRR32 pKa = 11.84IALDD36 pKa = 3.53RR37 pKa = 11.84YY38 pKa = 9.51VADD41 pKa = 3.49SYY43 pKa = 11.95DD44 pKa = 4.04YY45 pKa = 10.0FTVAHH50 pKa = 6.39AAEE53 pKa = 5.1GDD55 pKa = 3.9SFDD58 pKa = 3.53ITNGNGEE65 pKa = 4.7LIATEE70 pKa = 4.38SAFLYY75 pKa = 9.83PGIYY79 pKa = 9.54EE80 pKa = 4.31DD81 pKa = 2.96VWLIVDD87 pKa = 4.79DD88 pKa = 4.23YY89 pKa = 11.64GPNSKK94 pKa = 10.36EE95 pKa = 3.85GLVVTILLPEE105 pKa = 4.73EE106 pKa = 4.4YY107 pKa = 10.78

Molecular weight: 11.98 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0U2M345|A0A0U2M345_9BACL HTH-type transcriptional regulator KipR OS=Paenibacillus naphthalenovorans OX=162209 GN=IJ22_14160 PE=4 SV=1
MM1 pKa = 7.56QEE3 pKa = 3.64NTVQKK8 pKa = 10.05SANKK12 pKa = 7.81GTVIASVTTASMFAGILLIPLLLQEE37 pKa = 4.31VLHH40 pKa = 5.88YY41 pKa = 10.54SALAMGLILLPQALAMGLAMGLAMTVGGRR70 pKa = 11.84IVDD73 pKa = 3.36RR74 pKa = 11.84HH75 pKa = 4.9GAGWILPLGLLTVSCMSIALGFTAGQSSLWALVVQLSFRR114 pKa = 11.84GLGLGPIHH122 pKa = 6.37TPAMTVGLNALPFRR136 pKa = 11.84QVSRR140 pKa = 11.84AMSMNHH146 pKa = 5.28VAAQITASVAVVLFSLFFEE165 pKa = 5.07AKK167 pKa = 9.02RR168 pKa = 11.84SVYY171 pKa = 10.53ALLMPVQEE179 pKa = 4.65AGVLAVQQLFLAMGVMVLFAVPVSLRR205 pKa = 11.84WMRR208 pKa = 11.84KK209 pKa = 5.73KK210 pKa = 10.12HH211 pKa = 6.47RR212 pKa = 11.84GQLDD216 pKa = 3.35AACACKK222 pKa = 10.22RR223 pKa = 3.58

Molecular weight: 23.7 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

5049

0

5049

1504056

29

2803

297.9

33.21

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.263 ± 0.046

0.821 ± 0.011

4.943 ± 0.027

6.884 ± 0.037

4.041 ± 0.025

7.513 ± 0.031

2.187 ± 0.016

6.869 ± 0.029

5.705 ± 0.035

10.078 ± 0.043

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.85 ± 0.018

3.639 ± 0.028

4.216 ± 0.023

3.882 ± 0.022

5.276 ± 0.033

5.92 ± 0.026

5.16 ± 0.029

7.211 ± 0.026

1.201 ± 0.013

3.341 ± 0.019
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski