Streptococcus phage Javan522

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; unclassified Siphoviridae

Average proteome isoelectric point is 6.59

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 60 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A4D6B8Q2|A0A4D6B8Q2_9CAUD Capsid protein OS=Streptococcus phage Javan522 OX=2548235 GN=Javan522_0040 PE=4 SV=1
MM1 pKa = 7.82LIYY4 pKa = 10.15PATFTQDD11 pKa = 2.07TDD13 pKa = 4.11YY14 pKa = 11.77IMVTFPDD21 pKa = 3.7VPEE24 pKa = 5.63AITQGEE30 pKa = 4.54DD31 pKa = 3.17FQEE34 pKa = 4.6AYY36 pKa = 10.36EE37 pKa = 4.17MAVEE41 pKa = 4.15VLGFALEE48 pKa = 5.25DD49 pKa = 3.43YY50 pKa = 10.53TDD52 pKa = 3.83YY53 pKa = 11.38PKK55 pKa = 11.13ACAVSDD61 pKa = 4.43LKK63 pKa = 10.98EE64 pKa = 3.99QYY66 pKa = 9.46PDD68 pKa = 3.46SDD70 pKa = 3.42IALIGIDD77 pKa = 3.16MVAYY81 pKa = 7.98MKK83 pKa = 10.29KK84 pKa = 8.58YY85 pKa = 9.49HH86 pKa = 5.88SKK88 pKa = 10.45KK89 pKa = 8.6VRR91 pKa = 11.84KK92 pKa = 9.32NVTIPEE98 pKa = 4.28WLNDD102 pKa = 3.5AAEE105 pKa = 4.41DD106 pKa = 3.75KK107 pKa = 10.95NLNFSQVLTEE117 pKa = 4.09ALEE120 pKa = 4.63LKK122 pKa = 10.44LQAA125 pKa = 4.95

Molecular weight: 14.24 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A4D6BCQ4|A0A4D6BCQ4_9CAUD Uncharacterized protein OS=Streptococcus phage Javan522 OX=2548235 GN=Javan522_0022 PE=4 SV=1
MM1 pKa = 7.36TKK3 pKa = 7.85QHH5 pKa = 6.91RR6 pKa = 11.84EE7 pKa = 3.47TLIWYY12 pKa = 7.66RR13 pKa = 11.84ASHH16 pKa = 5.21QEE18 pKa = 3.99RR19 pKa = 11.84EE20 pKa = 4.0RR21 pKa = 11.84LLDD24 pKa = 4.04FGLVDD29 pKa = 3.46KK30 pKa = 11.12ARR32 pKa = 11.84YY33 pKa = 7.09VTLLRR38 pKa = 11.84QLRR41 pKa = 11.84KK42 pKa = 9.91KK43 pKa = 10.48YY44 pKa = 10.73AII46 pKa = 4.1

Molecular weight: 5.73 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

60

0

60

12116

42

1307

201.9

22.8

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.049 ± 0.69

0.669 ± 0.108

6.71 ± 0.275

7.263 ± 0.321

3.871 ± 0.245

6.388 ± 0.244

1.321 ± 0.118

7.065 ± 0.284

9.128 ± 0.337

8.707 ± 0.279

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.377 ± 0.162

5.72 ± 0.202

2.666 ± 0.218

3.978 ± 0.201

4.16 ± 0.221

6.116 ± 0.304

5.976 ± 0.258

5.918 ± 0.247

1.081 ± 0.119

3.838 ± 0.279
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski