Natronorubrum sulfidifaciens JCM 14089

Taxonomy: cellular organisms; Archaea; Euryarchaeota; Stenosarchaea group; Halobacteria; Natrialbales; Natrialbaceae; Natronorubrum; Natronorubrum sulfidifaciens

Average proteome isoelectric point is 4.79

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3428 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|L9WD93|L9WD93_9EURY Uncharacterized protein OS=Natronorubrum sulfidifaciens JCM 14089 OX=1230460 GN=C495_03677 PE=4 SV=1
MM1 pKa = 7.44EE2 pKa = 4.86LTWHH6 pKa = 6.23GHH8 pKa = 4.48STWHH12 pKa = 4.69VTVGEE17 pKa = 4.21TALLIDD23 pKa = 4.8PFFDD27 pKa = 3.84NPKK30 pKa = 9.57TDD32 pKa = 5.02LEE34 pKa = 4.4PADD37 pKa = 4.78IDD39 pKa = 3.84TPDD42 pKa = 3.68YY43 pKa = 11.39VLLTHH48 pKa = 6.39GHH50 pKa = 7.03ADD52 pKa = 3.94HH53 pKa = 6.95IADD56 pKa = 4.27AGAFSDD62 pKa = 3.87ATLVATPEE70 pKa = 4.14LVSYY74 pKa = 10.45CQDD77 pKa = 2.84EE78 pKa = 4.78FGFEE82 pKa = 4.11DD83 pKa = 3.7AVGGMGMNLGGTVEE97 pKa = 4.54CGDD100 pKa = 3.9AFVTMVRR107 pKa = 11.84ADD109 pKa = 3.55HH110 pKa = 6.68TNGIMTEE117 pKa = 3.94NDD119 pKa = 3.09ASGGMPAGFVISDD132 pKa = 3.79TKK134 pKa = 8.88PTQVADD140 pKa = 3.87EE141 pKa = 4.58EE142 pKa = 4.71STAFYY147 pKa = 10.94NAGDD151 pKa = 3.83TSLMTEE157 pKa = 3.71MRR159 pKa = 11.84EE160 pKa = 4.05VVGPYY165 pKa = 10.16LEE167 pKa = 5.0PDD169 pKa = 3.25AAAVPIGDD177 pKa = 4.72HH178 pKa = 5.56FTMGPWQAAVAVDD191 pKa = 3.81WLDD194 pKa = 2.8VDD196 pKa = 4.01YY197 pKa = 11.12ALPQHH202 pKa = 6.94YY203 pKa = 7.94DD204 pKa = 3.16TFPPIEE210 pKa = 4.01QDD212 pKa = 3.09PEE214 pKa = 3.91EE215 pKa = 4.42FEE217 pKa = 5.27RR218 pKa = 11.84EE219 pKa = 4.14VKK221 pKa = 9.78ATGSDD226 pKa = 3.22ADD228 pKa = 3.95VVIAEE233 pKa = 4.38ADD235 pKa = 3.63EE236 pKa = 4.7PFDD239 pKa = 3.54II240 pKa = 5.69

Molecular weight: 25.9 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|L9W1D0|L9W1D0_9EURY Sodium/hydrogen exchanger OS=Natronorubrum sulfidifaciens JCM 14089 OX=1230460 GN=C495_16233 PE=4 SV=1
MM1 pKa = 7.52AKK3 pKa = 10.19KK4 pKa = 10.18SGHH7 pKa = 5.14FSMRR11 pKa = 11.84IVDD14 pKa = 4.49WFSMDD19 pKa = 3.19RR20 pKa = 11.84VFTKK24 pKa = 10.29FIFEE28 pKa = 4.19VLLPSTIILTRR39 pKa = 11.84QCNIYY44 pKa = 10.72NRR46 pKa = 11.84VICNRR51 pKa = 11.84IFLIYY56 pKa = 10.24ILMIHH61 pKa = 6.87RR62 pKa = 11.84DD63 pKa = 3.35VPRR66 pKa = 11.84AKK68 pKa = 10.39RR69 pKa = 11.84LVTVNCRR76 pKa = 11.84QQ77 pKa = 3.08

Molecular weight: 9.28 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3428

0

3428

967963

34

3351

282.4

30.8

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

10.444 ± 0.063

0.762 ± 0.012

8.567 ± 0.059

8.927 ± 0.066

3.254 ± 0.029

8.056 ± 0.042

2.112 ± 0.021

4.673 ± 0.03

1.85 ± 0.026

9.036 ± 0.05

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.797 ± 0.02

2.326 ± 0.023

4.561 ± 0.028

2.656 ± 0.026

6.184 ± 0.043

5.539 ± 0.029

6.802 ± 0.028

8.624 ± 0.041

1.105 ± 0.017

2.72 ± 0.022
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski