Pararhizobium antarcticum
Average proteome isoelectric point is 6.4
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 4832 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A657LPZ9|A0A657LPZ9_9RHIZ Chaperone protein DnaK OS=Pararhizobium antarcticum OX=1798805 GN=dnaK PE=2 SV=1
MM1 pKa = 7.49 NIKK4 pKa = 10.39 SLLLGSAAALAAVSGANAADD24 pKa = 4.6 AIVAAEE30 pKa = 4.47 PEE32 pKa = 3.93 PMEE35 pKa = 4.1 YY36 pKa = 10.8 VRR38 pKa = 11.84 VCDD41 pKa = 4.42 AFGTGYY47 pKa = 10.47 FYY49 pKa = 10.57 IPGTEE54 pKa = 3.83 TCLKK58 pKa = 9.4 IGGYY62 pKa = 9.79 VRR64 pKa = 11.84 TQINYY69 pKa = 10.57 DD70 pKa = 3.18 EE71 pKa = 5.15 DD72 pKa = 3.97 LTVNGADD79 pKa = 2.89 WDD81 pKa = 3.88 ARR83 pKa = 11.84 TRR85 pKa = 11.84 GYY87 pKa = 9.51 LTFAAKK93 pKa = 10.46 NDD95 pKa = 3.91 TEE97 pKa = 4.23 YY98 pKa = 9.71 GTLSGYY104 pKa = 10.48 INLQADD110 pKa = 3.85 DD111 pKa = 4.34 TNDD114 pKa = 3.46 TFLDD118 pKa = 4.36 GAWINIAGFDD128 pKa = 3.48 VGYY131 pKa = 10.12 FYY133 pKa = 11.44 NWWDD137 pKa = 3.63 DD138 pKa = 3.39 MGLSGEE144 pKa = 4.35 TDD146 pKa = 3.13 VSAGNLSNAIRR157 pKa = 11.84 YY158 pKa = 6.62 TYY160 pKa = 10.82 DD161 pKa = 3.01 GGTFKK166 pKa = 11.14 VGVAAEE172 pKa = 3.99 EE173 pKa = 4.06 LAGNGTNDD181 pKa = 3.62 DD182 pKa = 4.08 VGVSGYY188 pKa = 10.15 VAGSIGGVTADD199 pKa = 4.66 LVASYY204 pKa = 11.12 DD205 pKa = 3.56 FDD207 pKa = 4.03 VEE209 pKa = 4.12 QFAVKK214 pKa = 10.11 GRR216 pKa = 11.84 LVAEE220 pKa = 4.87 IGPGALGILGVYY232 pKa = 10.44 ADD234 pKa = 4.18 GANFFWDD241 pKa = 3.48 VSEE244 pKa = 4.04 WSVAAEE250 pKa = 4.01 YY251 pKa = 10.28 KK252 pKa = 10.33 IQATEE257 pKa = 3.96 KK258 pKa = 10.8 FFITPAAQYY267 pKa = 10.06 FGDD270 pKa = 3.81 FAFVDD275 pKa = 5.16 GNDD278 pKa = 3.03 AWKK281 pKa = 10.71 VGVTAGYY288 pKa = 10.33 QITEE292 pKa = 4.19 GLRR295 pKa = 11.84 TLATVNYY302 pKa = 10.13 LDD304 pKa = 3.91 VDD306 pKa = 3.92 NVADD310 pKa = 4.24 GEE312 pKa = 4.47 WTGFVRR318 pKa = 11.84 LQRR321 pKa = 11.84 DD322 pKa = 3.62 FF323 pKa = 4.22
Molecular weight: 34.62 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.721
IPC2_protein 3.783
IPC_protein 3.808
Toseland 3.579
ProMoST 3.973
Dawson 3.808
Bjellqvist 3.961
Wikipedia 3.745
Rodwell 3.63
Grimsley 3.49
Solomon 3.795
Lehninger 3.757
Nozaki 3.91
DTASelect 4.177
Thurlkill 3.63
EMBOSS 3.757
Sillero 3.923
Patrickios 0.769
IPC_peptide 3.795
IPC2_peptide 3.91
IPC2.peptide.svr19 3.832
Protein with the highest isoelectric point:
>tr|A0A657LKY1|A0A657LKY1_9RHIZ Conjugal transfer protein TrbE OS=Pararhizobium antarcticum OX=1798805 GN=AX760_24775 PE=3 SV=1
MM1 pKa = 7.62 TKK3 pKa = 9.11 RR4 pKa = 11.84 TYY6 pKa = 10.36 QPSKK10 pKa = 9.73 LVRR13 pKa = 11.84 KK14 pKa = 8.95 RR15 pKa = 11.84 RR16 pKa = 11.84 HH17 pKa = 4.81 GFRR20 pKa = 11.84 SRR22 pKa = 11.84 IATKK26 pKa = 10.34 GGRR29 pKa = 11.84 KK30 pKa = 9.47 VITARR35 pKa = 11.84 RR36 pKa = 11.84 ARR38 pKa = 11.84 GRR40 pKa = 11.84 KK41 pKa = 9.03 RR42 pKa = 11.84 LSAA45 pKa = 4.03
Molecular weight: 5.29 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.449
IPC2_protein 11.155
IPC_protein 12.544
Toseland 12.705
ProMoST 13.203
Dawson 12.705
Bjellqvist 12.705
Wikipedia 13.188
Rodwell 12.398
Grimsley 12.749
Solomon 13.203
Lehninger 13.1
Nozaki 12.705
DTASelect 12.705
Thurlkill 12.705
EMBOSS 13.203
Sillero 12.705
Patrickios 12.135
IPC_peptide 13.203
IPC2_peptide 12.193
IPC2.peptide.svr19 9.082
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
4832
0
4832
1519063
41
2833
314.4
34.17
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
11.902 ± 0.047
0.834 ± 0.009
5.885 ± 0.028
5.5 ± 0.029
3.947 ± 0.025
8.352 ± 0.033
2.027 ± 0.016
5.808 ± 0.028
3.652 ± 0.029
9.959 ± 0.039
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.693 ± 0.016
2.804 ± 0.019
4.775 ± 0.025
3.14 ± 0.02
6.526 ± 0.033
5.794 ± 0.028
5.593 ± 0.022
7.279 ± 0.027
1.269 ± 0.013
2.261 ± 0.018
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here