Planifilum fulgidum

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Bacilli; Bacillales; Thermoactinomycetaceae; Planifilum

Average proteome isoelectric point is 7.06

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3217 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1I2MGJ7|A0A1I2MGJ7_9BACL Uncharacterized protein OS=Planifilum fulgidum OX=201973 GN=SAMN04488025_10818 PE=4 SV=1
MM1 pKa = 7.64AKK3 pKa = 8.89YY4 pKa = 9.46TMVDD8 pKa = 2.97KK9 pKa = 7.78EE10 pKa = 4.42TCIACGACGAAAPDD24 pKa = 3.19IFDD27 pKa = 4.34YY28 pKa = 11.17DD29 pKa = 4.39DD30 pKa = 4.21EE31 pKa = 5.7GLAEE35 pKa = 5.16NILEE39 pKa = 4.37GDD41 pKa = 3.87DD42 pKa = 3.48NRR44 pKa = 11.84GVVEE48 pKa = 4.45VPEE51 pKa = 4.01EE52 pKa = 4.61LYY54 pKa = 10.97DD55 pKa = 3.84QLEE58 pKa = 4.69DD59 pKa = 3.48AHH61 pKa = 7.1SGCPTGSIKK70 pKa = 10.33ISSQPMDD77 pKa = 4.24GDD79 pKa = 3.67PGKK82 pKa = 10.78AEE84 pKa = 3.97EE85 pKa = 4.2

Molecular weight: 9.01 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1I2NN53|A0A1I2NN53_9BACL Alanine racemase OS=Planifilum fulgidum OX=201973 GN=SAMN04488025_11370 PE=3 SV=1
MM1 pKa = 7.34YY2 pKa = 8.85PFCSGLAPCDD12 pKa = 3.24RR13 pKa = 11.84RR14 pKa = 11.84YY15 pKa = 11.11SSTNRR20 pKa = 11.84SISRR24 pKa = 11.84GVMPGLMRR32 pKa = 11.84RR33 pKa = 11.84ANSRR37 pKa = 11.84WQEE40 pKa = 3.76ANSRR44 pKa = 11.84PEE46 pKa = 3.47RR47 pKa = 11.84CIFSISALLLRR58 pKa = 11.84EE59 pKa = 4.0MVKK62 pKa = 10.01IKK64 pKa = 11.02YY65 pKa = 7.46PFPQYY70 pKa = 10.44HH71 pKa = 6.26YY72 pKa = 10.17PLLWDD77 pKa = 3.75EE78 pKa = 5.14RR79 pKa = 11.84KK80 pKa = 10.5GDD82 pKa = 3.72DD83 pKa = 3.56KK84 pKa = 11.23KK85 pKa = 11.13RR86 pKa = 11.84SGFSPVFVTKK96 pKa = 10.3MMEE99 pKa = 3.85RR100 pKa = 11.84HH101 pKa = 5.0NRR103 pKa = 11.84RR104 pKa = 11.84ARR106 pKa = 11.84VYY108 pKa = 10.91GRR110 pKa = 11.84GGCARR115 pKa = 11.84GPLLVAARR123 pKa = 11.84GFEE126 pKa = 4.49RR127 pKa = 11.84IWVIALIFFPLCFACDD143 pKa = 3.35TGQGFSGNGKK153 pKa = 9.61ARR155 pKa = 11.84SFFCAARR162 pKa = 11.84CILDD166 pKa = 3.29VANRR170 pKa = 11.84RR171 pKa = 11.84EE172 pKa = 4.13EE173 pKa = 4.19GGNRR177 pKa = 3.34

Molecular weight: 20.29 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3217

0

3217

940472

39

2177

292.3

32.68

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.594 ± 0.048

0.837 ± 0.014

4.9 ± 0.038

7.628 ± 0.055

3.983 ± 0.037

8.236 ± 0.044

2.105 ± 0.018

5.82 ± 0.032

4.852 ± 0.041

10.52 ± 0.054

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.589 ± 0.017

2.592 ± 0.027

4.91 ± 0.03

2.925 ± 0.03

7.865 ± 0.052

5.143 ± 0.029

4.575 ± 0.025

7.707 ± 0.035

1.425 ± 0.022

2.794 ± 0.025
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski