Thermomonospora umbrina

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Actinomycetia; Streptosporangiales; Thermomonosporaceae; Thermomonospora

Average proteome isoelectric point is 6.59

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 6565 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A3D9SSA7|A0A3D9SSA7_9ACTN Uncharacterized protein DUF4232 OS=Thermomonospora umbrina OX=111806 GN=DFJ69_1259 PE=4 SV=1
MM1 pKa = 7.15MMYY4 pKa = 9.98IVVPGLIVVVILVVVLVALGMRR26 pKa = 11.84ANRR29 pKa = 11.84TGADD33 pKa = 3.35EE34 pKa = 5.89DD35 pKa = 4.06DD36 pKa = 4.37WADD39 pKa = 4.46EE40 pKa = 4.56PPQQQPRR47 pKa = 11.84SRR49 pKa = 11.84GRR51 pKa = 11.84RR52 pKa = 11.84AAPPPVDD59 pKa = 3.72EE60 pKa = 5.18PVDD63 pKa = 4.23DD64 pKa = 4.94GYY66 pKa = 11.59EE67 pKa = 3.96DD68 pKa = 4.04HH69 pKa = 6.85GTGAGYY75 pKa = 10.25DD76 pKa = 3.22RR77 pKa = 11.84RR78 pKa = 11.84VAGGPLAAPTGPPAPAQPAGLAGPGPAGAPPGPPAPPPPAPRR120 pKa = 11.84RR121 pKa = 11.84APAGDD126 pKa = 3.89DD127 pKa = 3.53MSDD130 pKa = 3.06EE131 pKa = 4.67MGDD134 pKa = 3.49DD135 pKa = 4.94DD136 pKa = 4.56YY137 pKa = 11.54WATITFDD144 pKa = 3.31KK145 pKa = 11.02PKK147 pKa = 10.48FPWQHH152 pKa = 6.53DD153 pKa = 3.51RR154 pKa = 11.84DD155 pKa = 3.82EE156 pKa = 5.19DD157 pKa = 4.01RR158 pKa = 11.84DD159 pKa = 3.7EE160 pKa = 4.93AADD163 pKa = 3.96PLAAAADD170 pKa = 3.98MAEE173 pKa = 4.68PGAPQQPHH181 pKa = 6.62APAEE185 pKa = 3.98PAMAQPVPAGPMDD198 pKa = 3.43QPGYY202 pKa = 10.55GGDD205 pKa = 3.55SFGSDD210 pKa = 3.12GQYY213 pKa = 11.33GADD216 pKa = 3.13QYY218 pKa = 12.02GAGDD222 pKa = 3.36QYY224 pKa = 11.89GAGDD228 pKa = 3.47QYY230 pKa = 11.78ATGDD234 pKa = 3.43QYY236 pKa = 11.95GVEE239 pKa = 4.39QYY241 pKa = 11.19GADD244 pKa = 3.41QYY246 pKa = 12.02GGGGDD251 pKa = 3.69QYY253 pKa = 11.71ADD255 pKa = 3.32QYY257 pKa = 10.8GDD259 pKa = 3.36QYY261 pKa = 11.82ADD263 pKa = 3.45QPADD267 pKa = 3.18QGDD270 pKa = 4.13RR271 pKa = 11.84YY272 pKa = 10.57AADD275 pKa = 3.51GGRR278 pKa = 11.84YY279 pKa = 8.16PVEE282 pKa = 4.04PAPYY286 pKa = 8.97AAEE289 pKa = 4.27AGPYY293 pKa = 9.53GGEE296 pKa = 4.01PAASPGGYY304 pKa = 8.26GTGPQSVPAPPPSFPGTPEE323 pKa = 3.8PHH325 pKa = 7.27DD326 pKa = 4.34LPIAAHH332 pKa = 5.76FRR334 pKa = 11.84GDD336 pKa = 3.6AGDD339 pKa = 3.98PLATGGNPAGPTRR352 pKa = 11.84QDD354 pKa = 3.5PLDD357 pKa = 4.06YY358 pKa = 9.37ATPPPVSDD366 pKa = 3.98PLGLPVGRR374 pKa = 11.84ADD376 pKa = 4.4DD377 pKa = 4.12RR378 pKa = 11.84SSTGPFAGPGEE389 pKa = 4.46GSFGLPDD396 pKa = 3.9PPSYY400 pKa = 11.05GGAGEE405 pKa = 4.6GAGSTGYY412 pKa = 10.96GPTEE416 pKa = 4.03TTVSFSADD424 pKa = 3.47SPPAGPLHH432 pKa = 6.7GGFGDD437 pKa = 4.11SRR439 pKa = 11.84GLPRR443 pKa = 11.84NEE445 pKa = 3.99PSGAGPGGPPAHH457 pKa = 6.86SAPAAYY463 pKa = 9.49GSPAAPSGQQNDD475 pKa = 3.9TDD477 pKa = 3.84GHH479 pKa = 6.19RR480 pKa = 11.84LPTVDD485 pKa = 5.33EE486 pKa = 4.18LLQRR490 pKa = 11.84IQSDD494 pKa = 3.94RR495 pKa = 11.84QRR497 pKa = 11.84STGPTPVSGGNPLPDD512 pKa = 4.61PLNDD516 pKa = 4.18PLSTSHH522 pKa = 7.1DD523 pKa = 3.34ASYY526 pKa = 11.49GSGGAPWSSSTTGGSQGYY544 pKa = 8.34DD545 pKa = 3.09QPSSGRR551 pKa = 11.84GHH553 pKa = 7.76DD554 pKa = 4.34DD555 pKa = 3.31LSSGQLSGSGLSGGGFSGLGGGGSPGYY582 pKa = 10.02GQGGSGGQGQGYY594 pKa = 10.29ASGEE598 pKa = 4.47GYY600 pKa = 9.92PSAPAYY606 pKa = 10.52GEE608 pKa = 3.92APRR611 pKa = 11.84YY612 pKa = 9.44DD613 pKa = 4.14DD614 pKa = 4.61PLGGGPSSGRR624 pKa = 11.84EE625 pKa = 3.93SFGSFGMGEE634 pKa = 4.43PSTPPASGGSADD646 pKa = 3.58PGRR649 pKa = 11.84YY650 pKa = 9.67GDD652 pKa = 4.46FSGSNYY658 pKa = 10.6GGGDD662 pKa = 3.58PNATQAFGNGYY673 pKa = 10.32FGGQQGGHH681 pKa = 5.35GQADD685 pKa = 3.21QGTPQGEE692 pKa = 4.16QGSGYY697 pKa = 9.7GQQDD701 pKa = 3.21QRR703 pKa = 11.84GGDD706 pKa = 3.42DD707 pKa = 3.49WEE709 pKa = 4.13SHH711 pKa = 5.64RR712 pKa = 11.84DD713 pKa = 3.49YY714 pKa = 11.28RR715 pKa = 11.84RR716 pKa = 2.95

Molecular weight: 71.75 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A3D9SQA3|A0A3D9SQA3_9ACTN Putative cold-shock DNA-binding protein OS=Thermomonospora umbrina OX=111806 GN=DFJ69_2104 PE=4 SV=1
MM1 pKa = 7.4GSVIKK6 pKa = 10.42KK7 pKa = 8.47RR8 pKa = 11.84RR9 pKa = 11.84KK10 pKa = 9.22RR11 pKa = 11.84MAKK14 pKa = 9.41KK15 pKa = 9.99KK16 pKa = 9.77HH17 pKa = 5.81RR18 pKa = 11.84KK19 pKa = 8.51LLKK22 pKa = 8.15KK23 pKa = 9.25TRR25 pKa = 11.84VQRR28 pKa = 11.84RR29 pKa = 11.84NKK31 pKa = 9.81KK32 pKa = 9.63

Molecular weight: 4.02 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

6565

0

6565

2119970

29

4672

322.9

34.69

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

13.193 ± 0.045

0.787 ± 0.008

6.146 ± 0.023

5.729 ± 0.03

2.692 ± 0.017

9.648 ± 0.039

2.275 ± 0.014

3.305 ± 0.019

1.789 ± 0.023

10.283 ± 0.043

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.833 ± 0.012

1.67 ± 0.016

6.501 ± 0.035

2.401 ± 0.017

9.031 ± 0.033

4.74 ± 0.024

5.855 ± 0.025

8.656 ± 0.028

1.529 ± 0.012

1.939 ± 0.014
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski