Sphingomonas sp. HMF7854
Average proteome isoelectric point is 6.79
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 3013 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A3S0ELD5|A0A3S0ELD5_9SPHN FMN-binding negative transcriptional regulator OS=Sphingomonas sp. HMF7854 OX=2315330 GN=HMF7854_05065 PE=4 SV=1
MM1 pKa = 7.44 TYY3 pKa = 10.66 EE4 pKa = 4.62 DD5 pKa = 3.82 ASKK8 pKa = 11.26 APVAHH13 pKa = 7.46 DD14 pKa = 4.3 DD15 pKa = 3.4 VDD17 pKa = 5.08 AIPARR22 pKa = 11.84 SHH24 pKa = 5.67 TLATGNAITGAGTVSGAAGADD45 pKa = 3.36 LVGPGGHH52 pKa = 5.79 IVMVQGAGGEE62 pKa = 4.12 NGDD65 pKa = 3.85 LAGGRR70 pKa = 11.84 LHH72 pKa = 7.01 VAGAYY77 pKa = 8.22 GALAIAPNGQYY88 pKa = 10.51 SYY90 pKa = 10.19 TPNRR94 pKa = 11.84 GAPDD98 pKa = 3.81 GVADD102 pKa = 4.31 RR103 pKa = 11.84 FIYY106 pKa = 10.02 TLADD110 pKa = 3.24 AGGATDD116 pKa = 5.38 KK117 pKa = 10.97 ATLTINLGRR126 pKa = 11.84 EE127 pKa = 4.23 PEE129 pKa = 4.45 VVRR132 pKa = 11.84 ADD134 pKa = 3.3 ARR136 pKa = 11.84 QVVPGPNGVVVLPAGVTLNDD156 pKa = 3.61 VHH158 pKa = 7.16 VHH160 pKa = 5.62 GADD163 pKa = 4.11 LVIDD167 pKa = 4.68 LPDD170 pKa = 3.91 GTQMIIPNGAVFVPQLVIDD189 pKa = 4.26 DD190 pKa = 4.45 VEE192 pKa = 4.55 VPSTNLAALLIDD204 pKa = 4.38 QEE206 pKa = 4.51 PKK208 pKa = 9.77 PASGTPQSSGGNFEE222 pKa = 4.83 VPVPPLDD229 pKa = 4.09 PGVPLGDD236 pKa = 4.13 LLPPTEE242 pKa = 4.29 LTYY245 pKa = 10.53 TPPEE249 pKa = 4.05 FRR251 pKa = 11.84 DD252 pKa = 3.12 VGQFIDD258 pKa = 4.07 RR259 pKa = 11.84 EE260 pKa = 4.13 PSVVIDD266 pKa = 4.67 TPDD269 pKa = 3.13 QPAGAVEE276 pKa = 4.04 ATATVNEE283 pKa = 4.36 AGLPARR289 pKa = 11.84 TIGGKK294 pKa = 9.93 AEE296 pKa = 4.03 PAGSNAAANSEE307 pKa = 4.49 TTSGTIVFDD316 pKa = 4.21 SGDD319 pKa = 3.69 APATLTVNGVAITAVGQVLHH339 pKa = 6.09 GQYY342 pKa = 11.57 GDD344 pKa = 3.5 MTVTSIGAGTVGYY357 pKa = 10.4 SYY359 pKa = 10.39 TLADD363 pKa = 4.01 NSAGDD368 pKa = 3.53 ATKK371 pKa = 10.8 DD372 pKa = 3.3 VFTVVVTDD380 pKa = 3.96 RR381 pKa = 11.84 DD382 pKa = 3.77 GDD384 pKa = 3.89 SATAHH389 pKa = 5.66 LTVSIVDD396 pKa = 3.98 DD397 pKa = 4.23 VPTARR402 pKa = 11.84 ADD404 pKa = 3.72 VDD406 pKa = 3.86 SLAAGSYY413 pKa = 10.53 GPEE416 pKa = 3.96 TGNVIIGAGTAGGAGGAGADD436 pKa = 3.54 TLGADD441 pKa = 4.39 GAALTAVTGFAGSSDD456 pKa = 3.54 SSFDD460 pKa = 3.36 AAGNLVVNGQHH471 pKa = 6.29 GVLTIKK477 pKa = 10.88 ADD479 pKa = 3.42 GSYY482 pKa = 10.66 SYY484 pKa = 11.48 ARR486 pKa = 11.84 NPGTAGGVSDD496 pKa = 4.08 SFTYY500 pKa = 10.56 TLTDD504 pKa = 3.26 GDD506 pKa = 4.96 GDD508 pKa = 3.98 TSSATLTINIGDD520 pKa = 4.19 ARR522 pKa = 11.84 PLAGPNAPVQLDD534 pKa = 3.56 DD535 pKa = 5.16 DD536 pKa = 4.6 ALAGGNPGGVGDD548 pKa = 4.7 VSPDD552 pKa = 3.28 SANLSGTLAASGGDD566 pKa = 3.53 GPLAYY571 pKa = 10.37 ALTLGGAPAGFTYY584 pKa = 10.79 VAGANGALLVQQNGTTVLTVTLNAATGAYY613 pKa = 9.86 SVTQNAPILHH623 pKa = 6.56 AAGGDD628 pKa = 3.6 EE629 pKa = 4.3 NNASFTIGYY638 pKa = 8.95 SATDD642 pKa = 3.55 KK643 pKa = 11.49 DD644 pKa = 3.56 GDD646 pKa = 3.95 AATGALTINVNDD658 pKa = 3.92 DD659 pKa = 3.42 TPTIGRR665 pKa = 11.84 ADD667 pKa = 3.57 LAAPVLSVDD676 pKa = 3.95 EE677 pKa = 4.51 TNLAANASAGFAALFVPHH695 pKa = 7.06 LGADD699 pKa = 4.08 GAGSTSYY706 pKa = 11.17 ALSVVAGADD715 pKa = 3.06 SGLIDD720 pKa = 3.52 VATGQKK726 pKa = 9.61 IVLTSNNGVVEE737 pKa = 3.87 GHH739 pKa = 6.2 VGSAAGAIAFTVSVNAATGTVTLDD763 pKa = 3.04 QVRR766 pKa = 11.84 ALSHH770 pKa = 7.27 PDD772 pKa = 2.85 ATNPDD777 pKa = 4.15 DD778 pKa = 4.59 AVSPAAGSIALVATVTDD795 pKa = 3.86 KK796 pKa = 11.69 DD797 pKa = 3.27 GDD799 pKa = 4.06 AASLSLNIGSSLVFHH814 pKa = 7.8 DD815 pKa = 5.51 DD816 pKa = 3.44 GPSIVGSDD824 pKa = 3.54 ATPPALAVDD833 pKa = 4.72 EE834 pKa = 4.62 STLGADD840 pKa = 4.23 ASASFANLFAANFGADD856 pKa = 3.4 GAGPTVYY863 pKa = 10.42 GLHH866 pKa = 5.95 VVDD869 pKa = 5.92 GSDD872 pKa = 3.64 SGLIDD877 pKa = 3.53 VATGEE882 pKa = 4.78 HH883 pKa = 6.37 IFLFNLDD890 pKa = 3.69 GAVVGLVGGSFEE902 pKa = 4.64 GEE904 pKa = 3.71 PAVAFTLSLDD914 pKa = 3.72 PATGIATLNQSRR926 pKa = 11.84 ALAHH930 pKa = 6.62 PNAGDD935 pKa = 3.71 PNDD938 pKa = 4.07 AVSPAAEE945 pKa = 5.32 LIQITATITDD955 pKa = 3.78 RR956 pKa = 11.84 DD957 pKa = 3.89 GDD959 pKa = 4.21 SATASHH965 pKa = 7.71 DD966 pKa = 3.27 IGSALLFLDD975 pKa = 5.11 DD976 pKa = 4.82 GPSFARR982 pKa = 11.84 TDD984 pKa = 3.8 ALPEE988 pKa = 3.83 TLMVDD993 pKa = 3.79 EE994 pKa = 5.21 TSLATDD1000 pKa = 3.16 AHH1002 pKa = 6.35 GNVAFLFDD1010 pKa = 4.25 GFYY1013 pKa = 10.88 GADD1016 pKa = 3.48 GAGSIHH1022 pKa = 6.59 YY1023 pKa = 10.44 SLVATNGSDD1032 pKa = 3.3 SGLVDD1037 pKa = 3.47 VATGQHH1043 pKa = 5.69 IFLFVNGGGVEE1054 pKa = 4.03 GHH1056 pKa = 6.44 VGNSAGGVLAFTVSLDD1072 pKa = 3.55 SDD1074 pKa = 3.97 SGDD1077 pKa = 3.28 VTLDD1081 pKa = 3.19 QIRR1084 pKa = 11.84 ALSHH1088 pKa = 6.97 PDD1090 pKa = 3.19 AADD1093 pKa = 3.27 SDD1095 pKa = 4.79 DD1096 pKa = 3.97 AVSPLVGTIGVTATITDD1113 pKa = 3.49 GDD1115 pKa = 4.23 GDD1117 pKa = 4.36 SVATSVDD1124 pKa = 3.39 LSGALVFHH1132 pKa = 7.8 DD1133 pKa = 4.94 DD1134 pKa = 3.91 GPRR1137 pKa = 11.84 AAADD1141 pKa = 3.51 VNSVSEE1147 pKa = 4.33 GGSVSTTVLTGVLANDD1163 pKa = 3.54 TAGADD1168 pKa = 3.68 GLKK1171 pKa = 10.71 AGGAVVSAAAGGGSAISVGGSGGTVIAGQYY1201 pKa = 7.15 GTLTIFADD1209 pKa = 3.22 GHH1211 pKa = 5.44 YY1212 pKa = 10.16 AYY1214 pKa = 10.34 QSNPNSVSATVVDD1227 pKa = 3.83 HH1228 pKa = 6.27 FTYY1231 pKa = 10.6 TMRR1234 pKa = 11.84 DD1235 pKa = 3.18 GDD1237 pKa = 4.52 GDD1239 pKa = 4.22 PSTATLDD1246 pKa = 3.5 ITVDD1250 pKa = 3.79 PVDD1253 pKa = 4.78 LPAPAARR1260 pKa = 11.84 GTVFEE1265 pKa = 4.89 AALDD1269 pKa = 4.03 LLKK1272 pKa = 11.13 DD1273 pKa = 4.0 GSDD1276 pKa = 3.41 LAAGTVTGSNPGSTGEE1292 pKa = 4.44 TVGGTLLNPAADD1304 pKa = 3.8 HH1305 pKa = 6.06 YY1306 pKa = 11.84 AAFTQTTPYY1315 pKa = 10.96 GVFSIDD1321 pKa = 4.56 AAGHH1325 pKa = 4.16 WTFTLTSPVLGPQANDD1341 pKa = 3.47 GANPQIPVQHH1351 pKa = 6.36 FSYY1354 pKa = 10.46 TVFDD1358 pKa = 4.65 ANGNSATGSIDD1369 pKa = 3.2 VTIVDD1374 pKa = 5.31 DD1375 pKa = 4.38 VPQVTNVTAANGVTLDD1391 pKa = 3.6 EE1392 pKa = 4.32 TAGAVAGAAGGMTAISATSAGAVITATQLFGADD1425 pKa = 3.53 GPAASGAVAYY1435 pKa = 9.73 GVALANGLGSEE1446 pKa = 4.16 ATGLKK1451 pKa = 8.87 TAVGDD1456 pKa = 3.71 FAITLVQVDD1465 pKa = 3.94 ATHH1468 pKa = 7.49 IAGTYY1473 pKa = 9.75 NGASVAFTLAINADD1487 pKa = 3.42 GSITVVQNVALEE1499 pKa = 4.14 HH1500 pKa = 6.8 SEE1502 pKa = 4.08 TSDD1505 pKa = 3.66 PNDD1508 pKa = 3.78 ALSLAGLVDD1517 pKa = 3.43 ATITIKK1523 pKa = 11.17 DD1524 pKa = 3.43 GDD1526 pKa = 3.82 GDD1528 pKa = 4.02 IASGAAGIGQLVTFRR1543 pKa = 11.84 DD1544 pKa = 3.88 DD1545 pKa = 3.63 GPAVSVTAGADD1556 pKa = 3.09 AGVTLTTHH1564 pKa = 7.22 DD1565 pKa = 4.33 ALTIGAASDD1574 pKa = 4.09 SVSSSASFGGLFGATSTGGADD1595 pKa = 3.83 GAAGPAWSFALGVTTGASGLTSHH1618 pKa = 6.95 GAAINLYY1625 pKa = 9.25 LVNGVVLGSTAGTAPAGAGDD1645 pKa = 3.9 PSVVFSVAVSGAGVVTLTQYY1665 pKa = 11.08 SQIDD1669 pKa = 3.64 HH1670 pKa = 6.79 SLPGATGSFDD1680 pKa = 3.64 SQIAAILNDD1689 pKa = 3.65 HH1690 pKa = 7.32 AITLTGTATVTDD1702 pKa = 4.02 GDD1704 pKa = 4.08 GDD1706 pKa = 3.65 QATGSKK1712 pKa = 9.72 TIDD1715 pKa = 3.12 IANNLHH1721 pKa = 6.34 FADD1724 pKa = 5.0 AGPSITAGYY1733 pKa = 9.46 FLQQGIVADD1742 pKa = 4.1 EE1743 pKa = 4.68 SGPSTAQAIALSGEE1757 pKa = 3.92 ITAGNDD1763 pKa = 3.1 PDD1765 pKa = 4.02 VAGSGAIGHH1774 pKa = 6.46 ATGSVPFVAAFPDD1787 pKa = 5.02 FGADD1791 pKa = 3.34 GPAASGSLVYY1801 pKa = 10.79 GFTILNDD1808 pKa = 3.89 GASGLTTSEE1817 pKa = 3.94 GTPILLQLLANGVVAGVVQGTSTAAFAIAIDD1848 pKa = 3.97 PATGLVSLEE1857 pKa = 4.03 QYY1859 pKa = 11.07 LSIAHH1864 pKa = 6.77 SDD1866 pKa = 3.47 PTNGNDD1872 pKa = 3.09 VTRR1875 pKa = 11.84 LVNGSLGVTATATDD1889 pKa = 3.68 FDD1891 pKa = 4.95 GDD1893 pKa = 4.09 HH1894 pKa = 7.2 ASATLDD1900 pKa = 3.19 VSLYY1904 pKa = 10.79 LQFRR1908 pKa = 11.84 DD1909 pKa = 4.95 DD1910 pKa = 4.38 APQGAADD1917 pKa = 3.97 SNSVSEE1923 pKa = 4.59 GGTVATTAATGVLVNDD1939 pKa = 4.16 ASGADD1944 pKa = 4.1 GYY1946 pKa = 10.58 RR1947 pKa = 11.84 AAGAVVLAGTAAGGSVTAVSGGGTAIAGLYY1977 pKa = 7.49 GTLTIYY1983 pKa = 11.29 ADD1985 pKa = 3.31 GHH1987 pKa = 5.88 YY1988 pKa = 10.31 SYY1990 pKa = 11.04 QSNPNAVSGTQVDD2003 pKa = 3.75 HH2004 pKa = 6.74 FVYY2007 pKa = 10.05 TIEE2010 pKa = 5.46 DD2011 pKa = 3.63 RR2012 pKa = 11.84 DD2013 pKa = 3.77 GDD2015 pKa = 3.84 RR2016 pKa = 11.84 STTTLDD2022 pKa = 2.89 ITINDD2027 pKa = 3.74 VSNPAQNQSGTVFEE2041 pKa = 4.81 AALDD2045 pKa = 3.96 FAKK2048 pKa = 10.8 DD2049 pKa = 3.57 GADD2052 pKa = 3.49 LAVGTATGSNPGSTGEE2068 pKa = 4.45 TVSGTLANAFAAPGGYY2084 pKa = 8.7 VAQHH2088 pKa = 6.02 IVTANYY2094 pKa = 10.14 VFDD2097 pKa = 5.67 LDD2099 pKa = 5.3 VNGHH2103 pKa = 4.5 WTFTLTSPLHH2113 pKa = 6.41 SGTAADD2119 pKa = 4.12 GANVEE2124 pKa = 4.41 ASFSFSYY2131 pKa = 10.77 AVADD2135 pKa = 3.72 ALGNVSNGSIAVSVVDD2151 pKa = 4.94 DD2152 pKa = 3.98 VPTARR2157 pKa = 11.84 GDD2159 pKa = 3.62 TDD2161 pKa = 3.7 ALAAGSYY2168 pKa = 10.37 GPEE2171 pKa = 4.09 AGNVITGTGTLEE2183 pKa = 4.11 GTGGAGTDD2191 pKa = 3.22 TRR2193 pKa = 11.84 GADD2196 pKa = 3.48 GAAVSSLTGTGAADD2210 pKa = 3.48 TSFDD2214 pKa = 3.34 ASGNLSVAGLYY2225 pKa = 7.61 GTLTMKK2231 pKa = 10.88 ADD2233 pKa = 3.24 GSYY2236 pKa = 10.86 AYY2238 pKa = 8.72 TRR2240 pKa = 11.84 NPGTPGGVDD2249 pKa = 3.51 DD2250 pKa = 4.42 VFHH2253 pKa = 6.1 YY2254 pKa = 10.02 TLTDD2258 pKa = 3.23 GDD2260 pKa = 4.55 GDD2262 pKa = 3.89 VSSATLTIHH2271 pKa = 6.46 VGDD2274 pKa = 4.55 AAPVTAANAAVQLDD2288 pKa = 3.97 DD2289 pKa = 5.22 DD2290 pKa = 4.8 ALAGGNPGGTGDD2302 pKa = 4.32 VNPDD2306 pKa = 3.08 AANLTGTLAASGGDD2320 pKa = 4.09 GPLSWALSLSGAPAGFAYY2338 pKa = 10.49 VAGPGGSLLIQQGGVTVITVTVNATTGGYY2367 pKa = 7.77 TVTQNAPISHH2377 pKa = 7.26 PAGGTEE2383 pKa = 4.07 NNVSFTIGYY2392 pKa = 6.79 TATDD2396 pKa = 3.48 QDD2398 pKa = 3.78 SDD2400 pKa = 4.19 SANGTLIINVNDD2412 pKa = 3.64 DD2413 pKa = 3.69 TPTVVVTADD2422 pKa = 2.85 VDD2424 pKa = 3.82 TGVVLTTHH2432 pKa = 7.29 DD2433 pKa = 3.69 AATIGAASDD2442 pKa = 3.79 TASSSANFGGIFHH2455 pKa = 7.39 LAFSAGADD2463 pKa = 3.63 GTAAAPTLGFTLHH2476 pKa = 5.69 ATALASGLTQGGAAINLFEE2495 pKa = 5.89 IGGKK2499 pKa = 9.47 VVGSTAATIGAVTAANTVFDD2519 pKa = 4.14 VGVGSTGMVTLTQYY2533 pKa = 11.0 SQVDD2537 pKa = 3.85 HH2538 pKa = 7.3 PLPGATSGYY2547 pKa = 9.83 AAQEE2551 pKa = 3.85 VSLADD2556 pKa = 3.74 GLITLTGAASITDD2569 pKa = 3.67 KK2570 pKa = 11.58 DD2571 pKa = 3.77 GDD2573 pKa = 4.05 TATQSQTVAIGSNLHH2588 pKa = 6.3 FADD2591 pKa = 5.28 DD2592 pKa = 4.58 GPAISATLTGTQIVIDD2608 pKa = 4.01 EE2609 pKa = 4.46 TDD2611 pKa = 3.32 GVAAAGGEE2619 pKa = 4.04 VDD2621 pKa = 3.96 VAGGNLGTVTMAAASLFTVTQGHH2644 pKa = 5.77 VSADD2648 pKa = 3.8 GQSLGYY2654 pKa = 10.96 ALALSSNGVNSGLLQSAANQPIFLYY2679 pKa = 11.09 SMGGTVVGSTSATAGGVTSGNTVFTVAINAATGAVTLTQFQAIEE2723 pKa = 4.09 HH2724 pKa = 6.55 GNTGSVNEE2732 pKa = 4.19 ASAAILAGLLNVVVTATDD2750 pKa = 3.66 GDD2752 pKa = 4.46 GDD2754 pKa = 4.05 TATAAVDD2761 pKa = 3.69 LGSRR2765 pKa = 11.84 IVFLDD2770 pKa = 3.96 DD2771 pKa = 4.17 GPTLGTIQNGTANNNPASAVSVGTLHH2797 pKa = 7.08 FAAGSDD2803 pKa = 3.42 APAVVTAISVDD2814 pKa = 3.42 THH2816 pKa = 6.15 GATSGGHH2823 pKa = 6.55 AIVSSFAGGVLTGYY2837 pKa = 10.85 SDD2839 pKa = 3.73 VDD2841 pKa = 3.32 NSGTVSAGDD2850 pKa = 3.38 TAVFTLTVNPAAGSSGQYY2868 pKa = 10.5 VFDD2871 pKa = 5.0 LLAPLDD2877 pKa = 3.93 PTVTDD2882 pKa = 3.75 VPIGGSSSFGAGPTQSQTLTDD2903 pKa = 3.77 GGSANLSVVSGYY2915 pKa = 10.74 LAGAGFNEE2923 pKa = 4.58 ASWMSTGVASGLTLSSVNGSTAGWGVANNNFEE2955 pKa = 4.11 GTAEE2959 pKa = 3.9 FFNWDD2964 pKa = 3.76 FGSGALDD2971 pKa = 3.48 TQGGVFTPPAGVTLPPITYY2990 pKa = 10.48 AVFDD2994 pKa = 3.92 FVNYY2998 pKa = 10.54 SGGDD3002 pKa = 3.51 DD3003 pKa = 2.99 IKK3005 pKa = 11.42 YY3006 pKa = 9.3 VVHH3009 pKa = 5.57 YY3010 pKa = 9.36 TDD3012 pKa = 3.91 GSFVSGTVPSANLIGSGPNWTFTAAAGKK3040 pKa = 9.57 FIADD3044 pKa = 2.91 IEE3046 pKa = 4.5 MFTSGTAPGKK3056 pKa = 10.3 VDD3058 pKa = 3.85 LVSVGVTSTSLNKK3071 pKa = 10.47 SFDD3074 pKa = 3.47 FSLTLKK3080 pKa = 10.71 DD3081 pKa = 3.71 ADD3083 pKa = 3.88 GDD3085 pKa = 4.21 PVSGAFTMNIANGNTPSAPVAPVVLDD3111 pKa = 3.79 LGGDD3115 pKa = 3.79 GVHH3118 pKa = 6.1 FVSRR3122 pKa = 11.84 AAGVTYY3128 pKa = 10.22 DD3129 pKa = 3.81 YY3130 pKa = 11.31 AGDD3133 pKa = 3.72 GHH3135 pKa = 6.79 PVATAWAAPEE3145 pKa = 4.6 DD3146 pKa = 4.78 GILVRR3151 pKa = 11.84 DD3152 pKa = 3.74 ANGNGTVDD3160 pKa = 3.76 GAAEE3164 pKa = 3.73 FAFAHH3169 pKa = 6.91 DD3170 pKa = 4.58 GLSDD3174 pKa = 4.04 LQALAAQYY3182 pKa = 11.23 GPTLDD3187 pKa = 4.91 AADD3190 pKa = 3.93 ADD3192 pKa = 4.33 FARR3195 pKa = 11.84 FGVWQDD3201 pKa = 3.11 ANLDD3205 pKa = 3.69 GRR3207 pKa = 11.84 VDD3209 pKa = 3.18 AGEE3212 pKa = 4.07 YY3213 pKa = 10.21 HH3214 pKa = 6.8 SLAALGISSVSLVSDD3229 pKa = 4.79 GISYY3233 pKa = 10.05 VAANGEE3239 pKa = 4.34 VEE3241 pKa = 4.39 VAGTGSFVRR3250 pKa = 11.84 NGASFALADD3259 pKa = 3.69 AAFLTGSRR3267 pKa = 11.84 EE3268 pKa = 3.78 LAQLDD3273 pKa = 3.58 KK3274 pKa = 11.28 AAAANTAGTTVIAAVAAAAMAQLPAATAAPGHH3306 pKa = 6.48 APDD3309 pKa = 3.97 TAHH3312 pKa = 7.21 AVAALASATPVAPAPEE3328 pKa = 4.43 PATTVRR3334 pKa = 11.84 AMLSNEE3340 pKa = 3.87 SHH3342 pKa = 6.79 APAADD3347 pKa = 3.32 KK3348 pKa = 10.84 PEE3350 pKa = 4.28 PVASASHH3357 pKa = 5.86 GRR3359 pKa = 11.84 GGAEE3363 pKa = 3.79 PEE3365 pKa = 4.43 GVADD3369 pKa = 4.02 AVSAHH3374 pKa = 6.79 AEE3376 pKa = 4.02 PAASPSALDD3385 pKa = 4.28 AGSAMPAVHH3394 pKa = 6.96 GSPIVLAAAQLPMISAEE3411 pKa = 4.14 SLQALAGLGAEE3422 pKa = 4.94 GKK3424 pKa = 6.95 TTMVVGKK3431 pKa = 10.02 VIVDD3435 pKa = 3.82 ALHH3438 pKa = 6.68 GGGGAPSIDD3447 pKa = 3.69 AMLQAVGANHH3457 pKa = 7.55 DD3458 pKa = 4.03 AGANPALAALASPAAAAVPGWDD3480 pKa = 3.27 AGHH3483 pKa = 6.38 FAGLSTHH3490 pKa = 6.8 LPSIMEE3496 pKa = 4.2 AAGLHH3501 pKa = 5.64 QDD3503 pKa = 4.6 AIQPAAHH3510 pKa = 6.82 AAA3512 pKa = 3.42
Molecular weight: 342.39 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.7
IPC2_protein 3.694
IPC_protein 3.783
Toseland 3.528
ProMoST 3.961
Dawson 3.783
Bjellqvist 3.935
Wikipedia 3.757
Rodwell 3.592
Grimsley 3.427
Solomon 3.783
Lehninger 3.745
Nozaki 3.897
DTASelect 4.215
Thurlkill 3.592
EMBOSS 3.757
Sillero 3.91
Patrickios 1.901
IPC_peptide 3.783
IPC2_peptide 3.872
IPC2.peptide.svr19 3.798
Protein with the highest isoelectric point:
>tr|A0A429V250|A0A429V250_9SPHN Uncharacterized protein OS=Sphingomonas sp. HMF7854 OX=2315330 GN=HMF7854_15230 PE=4 SV=1
MM1 pKa = 7.45 KK2 pKa = 9.61 RR3 pKa = 11.84 TFQPSNLVRR12 pKa = 11.84 KK13 pKa = 8.96 RR14 pKa = 11.84 RR15 pKa = 11.84 HH16 pKa = 4.62 GFRR19 pKa = 11.84 SRR21 pKa = 11.84 MATVGGRR28 pKa = 11.84 KK29 pKa = 9.07 VLNARR34 pKa = 11.84 RR35 pKa = 11.84 ARR37 pKa = 11.84 GRR39 pKa = 11.84 KK40 pKa = 9.02 KK41 pKa = 10.61 LSAA44 pKa = 4.03
Molecular weight: 5.14 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.523
IPC2_protein 11.242
IPC_protein 12.837
Toseland 13.013
ProMoST 13.495
Dawson 13.013
Bjellqvist 12.998
Wikipedia 13.481
Rodwell 12.676
Grimsley 13.042
Solomon 13.495
Lehninger 13.408
Nozaki 13.013
DTASelect 12.998
Thurlkill 13.013
EMBOSS 13.51
Sillero 13.013
Patrickios 12.398
IPC_peptide 13.51
IPC2_peptide 12.486
IPC2.peptide.svr19 9.177
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
3013
0
3013
967107
29
3512
321.0
34.63
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
13.461 ± 0.074
0.747 ± 0.013
5.7 ± 0.036
5.499 ± 0.05
3.464 ± 0.028
9.069 ± 0.048
1.975 ± 0.021
4.434 ± 0.026
2.62 ± 0.037
10.479 ± 0.059
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.17 ± 0.021
2.396 ± 0.033
5.591 ± 0.039
3.197 ± 0.026
7.993 ± 0.061
5.397 ± 0.034
5.055 ± 0.043
7.254 ± 0.033
1.437 ± 0.02
2.062 ± 0.025
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here