Amino acid dipepetide frequency for Sphingomonas sp. HMF7854

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
21.09AlaAla: 21.09 ± 0.227
0.994AlaCys: 0.994 ± 0.034
7.352AlaAsp: 7.352 ± 0.088
8.141AlaGlu: 8.141 ± 0.117
4.107AlaPhe: 4.107 ± 0.073
12.354AlaGly: 12.354 ± 0.131
2.186AlaHis: 2.186 ± 0.05
6.146AlaIle: 6.146 ± 0.08
3.318AlaLys: 3.318 ± 0.08
14.509AlaLeu: 14.509 ± 0.169
3.619AlaMet: 3.619 ± 0.071
3.028AlaAsn: 3.028 ± 0.075
6.836AlaPro: 6.836 ± 0.098
4.091AlaGln: 4.091 ± 0.079
10.192AlaArg: 10.192 ± 0.116
6.866AlaSer: 6.866 ± 0.086
6.63AlaThr: 6.63 ± 0.103
9.182AlaVal: 9.182 ± 0.109
1.664AlaTrp: 1.664 ± 0.044
2.304AlaTyr: 2.304 ± 0.051
0.0AlaXaa: 0.0 ± 0.0
Cys
0.947CysAla: 0.947 ± 0.039
0.087CysCys: 0.087 ± 0.009
0.476CysAsp: 0.476 ± 0.023
0.388CysGlu: 0.388 ± 0.02
0.272CysPhe: 0.272 ± 0.017
0.824CysGly: 0.824 ± 0.035
0.205CysHis: 0.205 ± 0.015
0.312CysIle: 0.312 ± 0.019
0.146CysLys: 0.146 ± 0.012
0.645CysLeu: 0.645 ± 0.024
0.12CysMet: 0.12 ± 0.011
0.188CysAsn: 0.188 ± 0.014
0.432CysPro: 0.432 ± 0.021
0.181CysGln: 0.181 ± 0.013
0.594CysArg: 0.594 ± 0.026
0.513CysSer: 0.513 ± 0.024
0.376CysThr: 0.376 ± 0.018
0.478CysVal: 0.478 ± 0.021
0.115CysTrp: 0.115 ± 0.012
0.17CysTyr: 0.17 ± 0.013
0.0CysXaa: 0.0 ± 0.0
Asp
6.733AspAla: 6.733 ± 0.095
0.468AspCys: 0.468 ± 0.025
2.974AspAsp: 2.974 ± 0.066
3.734AspGlu: 3.734 ± 0.074
1.974AspPhe: 1.974 ± 0.045
5.534AspGly: 5.534 ± 0.104
1.331AspHis: 1.331 ± 0.036
2.171AspIle: 2.171 ± 0.054
1.478AspLys: 1.478 ± 0.046
5.736AspLeu: 5.736 ± 0.088
1.07AspMet: 1.07 ± 0.038
1.22AspAsn: 1.22 ± 0.037
3.895AspPro: 3.895 ± 0.062
2.053AspGln: 2.053 ± 0.048
5.375AspArg: 5.375 ± 0.091
2.452AspSer: 2.452 ± 0.052
2.351AspThr: 2.351 ± 0.058
3.786AspVal: 3.786 ± 0.069
1.105AspTrp: 1.105 ± 0.036
1.56AspTyr: 1.56 ± 0.043
0.0AspXaa: 0.0 ± 0.0
Glu
7.611GluAla: 7.611 ± 0.101
0.32GluCys: 0.32 ± 0.02
2.446GluAsp: 2.446 ± 0.054
3.142GluGlu: 3.142 ± 0.076
1.438GluPhe: 1.438 ± 0.039
4.298GluGly: 4.298 ± 0.073
1.188GluHis: 1.188 ± 0.035
2.553GluIle: 2.553 ± 0.059
1.708GluLys: 1.708 ± 0.046
5.836GluLeu: 5.836 ± 0.09
1.24GluMet: 1.24 ± 0.034
1.146GluAsn: 1.146 ± 0.037
3.087GluPro: 3.087 ± 0.062
2.624GluGln: 2.624 ± 0.061
5.859GluArg: 5.859 ± 0.094
2.169GluSer: 2.169 ± 0.054
2.913GluThr: 2.913 ± 0.058
3.794GluVal: 3.794 ± 0.074
0.794GluTrp: 0.794 ± 0.033
0.82GluTyr: 0.82 ± 0.031
0.0GluXaa: 0.0 ± 0.0
Phe
4.952PheAla: 4.952 ± 0.085
0.294PheCys: 0.294 ± 0.018
2.563PheAsp: 2.563 ± 0.057
1.909PheGlu: 1.909 ± 0.048
1.249PhePhe: 1.249 ± 0.044
3.524PheGly: 3.524 ± 0.064
0.73PheHis: 0.73 ± 0.027
1.287PheIle: 1.287 ± 0.039
0.773PheLys: 0.773 ± 0.027
3.121PheLeu: 3.121 ± 0.062
0.599PheMet: 0.599 ± 0.023
1.019PheAsn: 1.019 ± 0.032
1.526PhePro: 1.526 ± 0.038
0.925PheGln: 0.925 ± 0.029
2.294PheArg: 2.294 ± 0.053
1.855PheSer: 1.855 ± 0.05
1.915PheThr: 1.915 ± 0.044
2.697PheVal: 2.697 ± 0.059
0.513PheTrp: 0.513 ± 0.029
0.892PheTyr: 0.892 ± 0.032
0.0PheXaa: 0.0 ± 0.0
Gly
10.517GlyAla: 10.517 ± 0.136
0.886GlyCys: 0.886 ± 0.032
4.834GlyAsp: 4.834 ± 0.075
5.321GlyGlu: 5.321 ± 0.078
3.637GlyPhe: 3.637 ± 0.068
8.548GlyGly: 8.548 ± 0.155
1.941GlyHis: 1.941 ± 0.045
4.122GlyIle: 4.122 ± 0.068
2.922GlyLys: 2.922 ± 0.061
8.897GlyLeu: 8.897 ± 0.106
2.099GlyMet: 2.099 ± 0.05
2.153GlyAsn: 2.153 ± 0.063
4.052GlyPro: 4.052 ± 0.069
3.127GlyGln: 3.127 ± 0.061
7.296GlyArg: 7.296 ± 0.108
5.476GlySer: 5.476 ± 0.094
4.922GlyThr: 4.922 ± 0.08
6.09GlyVal: 6.09 ± 0.086
1.705GlyTrp: 1.705 ± 0.042
2.146GlyTyr: 2.146 ± 0.049
0.0GlyXaa: 0.0 ± 0.0
His
2.275HisAla: 2.275 ± 0.051
0.233HisCys: 0.233 ± 0.016
1.204HisAsp: 1.204 ± 0.035
1.065HisGlu: 1.065 ± 0.034
0.802HisPhe: 0.802 ± 0.029
2.073HisGly: 2.073 ± 0.045
0.59HisHis: 0.59 ± 0.027
0.73HisIle: 0.73 ± 0.027
0.393HisLys: 0.393 ± 0.022
2.015HisLeu: 2.015 ± 0.052
0.396HisMet: 0.396 ± 0.022
0.44HisAsn: 0.44 ± 0.02
1.336HisPro: 1.336 ± 0.041
0.597HisGln: 0.597 ± 0.026
1.697HisArg: 1.697 ± 0.043
1.013HisSer: 1.013 ± 0.037
0.548HisThr: 0.548 ± 0.024
1.401HisVal: 1.401 ± 0.038
0.403HisTrp: 0.403 ± 0.022
0.54HisTyr: 0.54 ± 0.023
0.0HisXaa: 0.0 ± 0.0
Ile
6.816IleAla: 6.816 ± 0.083
0.356IleCys: 0.356 ± 0.019
3.413IleAsp: 3.413 ± 0.065
3.052IleGlu: 3.052 ± 0.059
1.34IlePhe: 1.34 ± 0.035
4.603IleGly: 4.603 ± 0.071
0.803IleHis: 0.803 ± 0.033
1.629IleIle: 1.629 ± 0.045
0.951IleLys: 0.951 ± 0.036
3.727IleLeu: 3.727 ± 0.071
0.697IleMet: 0.697 ± 0.026
1.167IleAsn: 1.167 ± 0.037
2.076IlePro: 2.076 ± 0.053
1.048IleGln: 1.048 ± 0.035
3.191IleArg: 3.191 ± 0.062
2.232IleSer: 2.232 ± 0.047
2.024IleThr: 2.024 ± 0.052
3.873IleVal: 3.873 ± 0.071
0.443IleTrp: 0.443 ± 0.024
0.9IleTyr: 0.9 ± 0.034
0.0IleXaa: 0.0 ± 0.0
Lys
3.429LysAla: 3.429 ± 0.059
0.105LysCys: 0.105 ± 0.01
1.338LysAsp: 1.338 ± 0.042
1.151LysGlu: 1.151 ± 0.039
0.723LysPhe: 0.723 ± 0.029
2.261LysGly: 2.261 ± 0.059
0.453LysHis: 0.453 ± 0.024
1.204LysIle: 1.204 ± 0.044
0.916LysLys: 0.916 ± 0.036
3.028LysLeu: 3.028 ± 0.06
0.611LysMet: 0.611 ± 0.029
0.596LysAsn: 0.596 ± 0.03
1.77LysPro: 1.77 ± 0.046
0.906LysGln: 0.906 ± 0.035
2.083LysArg: 2.083 ± 0.048
1.395LysSer: 1.395 ± 0.044
1.364LysThr: 1.364 ± 0.045
2.05LysVal: 2.05 ± 0.049
0.332LysTrp: 0.332 ± 0.019
0.486LysTyr: 0.486 ± 0.024
0.0LysXaa: 0.0 ± 0.0
Leu
15.543LeuAla: 15.543 ± 0.167
0.726LeuCys: 0.726 ± 0.032
6.383LeuAsp: 6.383 ± 0.096
5.188LeuGlu: 5.188 ± 0.082
3.911LeuPhe: 3.911 ± 0.081
9.156LeuGly: 9.156 ± 0.114
1.925LeuHis: 1.925 ± 0.043
4.395LeuIle: 4.395 ± 0.06
2.942LeuLys: 2.942 ± 0.06
11.447LeuLeu: 11.447 ± 0.177
1.958LeuMet: 1.958 ± 0.048
2.33LeuAsn: 2.33 ± 0.055
5.897LeuPro: 5.897 ± 0.088
2.833LeuGln: 2.833 ± 0.049
7.432LeuArg: 7.432 ± 0.089
5.962LeuSer: 5.962 ± 0.074
5.393LeuThr: 5.393 ± 0.08
8.158LeuVal: 8.158 ± 0.106
1.302LeuTrp: 1.302 ± 0.045
1.905LeuTyr: 1.905 ± 0.047
0.0LeuXaa: 0.0 ± 0.0
Met
2.875MetAla: 2.875 ± 0.051
0.114MetCys: 0.114 ± 0.012
1.03MetAsp: 1.03 ± 0.038
0.879MetGlu: 0.879 ± 0.03
0.589MetPhe: 0.589 ± 0.023
1.662MetGly: 1.662 ± 0.045
0.398MetHis: 0.398 ± 0.018
1.191MetIle: 1.191 ± 0.033
0.802MetLys: 0.802 ± 0.027
2.459MetLeu: 2.459 ± 0.053
0.583MetMet: 0.583 ± 0.025
0.676MetAsn: 0.676 ± 0.027
1.291MetPro: 1.291 ± 0.037
0.615MetGln: 0.615 ± 0.027
1.629MetArg: 1.629 ± 0.043
1.397MetSer: 1.397 ± 0.038
1.506MetThr: 1.506 ± 0.039
1.528MetVal: 1.528 ± 0.043
0.21MetTrp: 0.21 ± 0.016
0.263MetTyr: 0.263 ± 0.017
0.0MetXaa: 0.0 ± 0.0
Asn
3.008AsnAla: 3.008 ± 0.061
0.195AsnCys: 0.195 ± 0.014
1.29AsnAsp: 1.29 ± 0.04
1.072AsnGlu: 1.072 ± 0.035
0.884AsnPhe: 0.884 ± 0.034
2.439AsnGly: 2.439 ± 0.063
0.456AsnHis: 0.456 ± 0.024
1.123AsnIle: 1.123 ± 0.036
0.568AsnLys: 0.568 ± 0.027
2.429AsnLeu: 2.429 ± 0.062
0.477AsnMet: 0.477 ± 0.022
0.644AsnAsn: 0.644 ± 0.034
1.664AsnPro: 1.664 ± 0.046
0.768AsnGln: 0.768 ± 0.03
1.932AsnArg: 1.932 ± 0.049
1.278AsnSer: 1.278 ± 0.047
0.981AsnThr: 0.981 ± 0.043
1.726AsnVal: 1.726 ± 0.044
0.37AsnTrp: 0.37 ± 0.02
0.657AsnTyr: 0.657 ± 0.028
0.0AsnXaa: 0.0 ± 0.0
Pro
8.006ProAla: 8.006 ± 0.109
0.326ProCys: 0.326 ± 0.018
3.712ProAsp: 3.712 ± 0.056
3.379ProGlu: 3.379 ± 0.059
1.987ProPhe: 1.987 ± 0.048
5.184ProGly: 5.184 ± 0.086
1.007ProHis: 1.007 ± 0.035
2.287ProIle: 2.287 ± 0.05
1.312ProLys: 1.312 ± 0.041
5.767ProLeu: 5.767 ± 0.095
1.115ProMet: 1.115 ± 0.037
1.256ProAsn: 1.256 ± 0.037
3.385ProPro: 3.385 ± 0.114
1.651ProGln: 1.651 ± 0.047
3.535ProArg: 3.535 ± 0.069
2.869ProSer: 2.869 ± 0.053
2.794ProThr: 2.794 ± 0.062
4.559ProVal: 4.559 ± 0.078
0.703ProTrp: 0.703 ± 0.028
1.078ProTyr: 1.078 ± 0.039
0.0ProXaa: 0.0 ± 0.0
Gln
4.245GlnAla: 4.245 ± 0.07
0.21GlnCys: 0.21 ± 0.018
1.347GlnAsp: 1.347 ± 0.04
1.253GlnGlu: 1.253 ± 0.039
1.062GlnPhe: 1.062 ± 0.033
2.546GlnGly: 2.546 ± 0.057
0.6GlnHis: 0.6 ± 0.024
1.448GlnIle: 1.448 ± 0.037
0.822GlnLys: 0.822 ± 0.032
3.718GlnLeu: 3.718 ± 0.066
0.741GlnMet: 0.741 ± 0.026
0.738GlnAsn: 0.738 ± 0.024
2.131GlnPro: 2.131 ± 0.046
1.421GlnGln: 1.421 ± 0.043
2.947GlnArg: 2.947 ± 0.064
1.752GlnSer: 1.752 ± 0.043
1.556GlnThr: 1.556 ± 0.045
2.446GlnVal: 2.446 ± 0.043
0.438GlnTrp: 0.438 ± 0.022
0.553GlnTyr: 0.553 ± 0.028
0.0GlnXaa: 0.0 ± 0.0
Arg
9.239ArgAla: 9.239 ± 0.128
0.578ArgCys: 0.578 ± 0.026
4.41ArgAsp: 4.41 ± 0.068
4.41ArgGlu: 4.41 ± 0.071
3.456ArgPhe: 3.456 ± 0.07
5.673ArgGly: 5.673 ± 0.099
1.817ArgHis: 1.817 ± 0.042
4.013ArgIle: 4.013 ± 0.072
1.921ArgLys: 1.921 ± 0.055
9.485ArgLeu: 9.485 ± 0.135
1.966ArgMet: 1.966 ± 0.046
1.815ArgAsn: 1.815 ± 0.045
4.309ArgPro: 4.309 ± 0.08
2.858ArgGln: 2.858 ± 0.055
7.095ArgArg: 7.095 ± 0.121
4.617ArgSer: 4.617 ± 0.069
3.78ArgThr: 3.78 ± 0.062
5.182ArgVal: 5.182 ± 0.08
1.446ArgTrp: 1.446 ± 0.045
1.857ArgTyr: 1.857 ± 0.045
0.0ArgXaa: 0.0 ± 0.0
Ser
6.848SerAla: 6.848 ± 0.096
0.434SerCys: 0.434 ± 0.021
3.051SerAsp: 3.051 ± 0.065
2.598SerGlu: 2.598 ± 0.048
2.263SerPhe: 2.263 ± 0.048
5.724SerGly: 5.724 ± 0.096
0.997SerHis: 0.997 ± 0.033
2.445SerIle: 2.445 ± 0.055
1.221SerLys: 1.221 ± 0.042
5.668SerLeu: 5.668 ± 0.083
1.18SerMet: 1.18 ± 0.038
1.368SerAsn: 1.368 ± 0.044
3.027SerPro: 3.027 ± 0.055
1.536SerGln: 1.536 ± 0.048
3.945SerArg: 3.945 ± 0.065
3.094SerSer: 3.094 ± 0.074
2.698SerThr: 2.698 ± 0.056
3.75SerVal: 3.75 ± 0.061
0.844SerTrp: 0.844 ± 0.033
1.279SerTyr: 1.279 ± 0.042
0.0SerXaa: 0.0 ± 0.0
Thr
6.428ThrAla: 6.428 ± 0.082
0.347ThrCys: 0.347 ± 0.02
2.672ThrAsp: 2.672 ± 0.057
2.121ThrGlu: 2.121 ± 0.047
1.627ThrPhe: 1.627 ± 0.049
5.09ThrGly: 5.09 ± 0.078
0.889ThrHis: 0.889 ± 0.032
2.811ThrIle: 2.811 ± 0.052
1.157ThrLys: 1.157 ± 0.037
5.723ThrLeu: 5.723 ± 0.087
1.076ThrMet: 1.076 ± 0.033
1.211ThrAsn: 1.211 ± 0.037
3.266ThrPro: 3.266 ± 0.066
1.38ThrGln: 1.38 ± 0.043
3.56ThrArg: 3.56 ± 0.066
2.855ThrSer: 2.855 ± 0.065
2.653ThrThr: 2.653 ± 0.07
4.097ThrVal: 4.097 ± 0.075
0.494ThrTrp: 0.494 ± 0.025
1.092ThrTyr: 1.092 ± 0.041
0.0ThrXaa: 0.0 ± 0.0
Val
10.244ValAla: 10.244 ± 0.122
0.495ValCys: 0.495 ± 0.024
4.429ValAsp: 4.429 ± 0.067
4.648ValGlu: 4.648 ± 0.063
2.001ValPhe: 2.001 ± 0.05
6.193ValGly: 6.193 ± 0.085
1.421ValHis: 1.421 ± 0.035
3.173ValIle: 3.173 ± 0.069
1.834ValLys: 1.834 ± 0.047
6.658ValLeu: 6.658 ± 0.085
1.461ValMet: 1.461 ± 0.038
1.928ValAsn: 1.928 ± 0.055
4.185ValPro: 4.185 ± 0.07
2.044ValGln: 2.044 ± 0.052
5.905ValArg: 5.905 ± 0.091
3.992ValSer: 3.992 ± 0.087
4.406ValThr: 4.406 ± 0.085
5.392ValVal: 5.392 ± 0.088
0.843ValTrp: 0.843 ± 0.032
1.288ValTyr: 1.288 ± 0.034
0.0ValXaa: 0.0 ± 0.0
Trp
1.307TrpAla: 1.307 ± 0.037
0.103TrpCys: 0.103 ± 0.011
0.707TrpAsp: 0.707 ± 0.025
0.548TrpGlu: 0.548 ± 0.021
0.538TrpPhe: 0.538 ± 0.026
0.966TrpGly: 0.966 ± 0.034
0.361TrpHis: 0.361 ± 0.019
0.649TrpIle: 0.649 ± 0.028
0.474TrpLys: 0.474 ± 0.024
1.894TrpLeu: 1.894 ± 0.054
0.337TrpMet: 0.337 ± 0.019
0.458TrpAsn: 0.458 ± 0.022
0.735TrpPro: 0.735 ± 0.027
0.618TrpGln: 0.618 ± 0.023
1.42TrpArg: 1.42 ± 0.04
0.98TrpSer: 0.98 ± 0.033
0.845TrpThr: 0.845 ± 0.031
0.867TrpVal: 0.867 ± 0.034
0.285TrpTrp: 0.285 ± 0.019
0.278TrpTyr: 0.278 ± 0.018
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.495TyrAla: 2.495 ± 0.056
0.196TyrCys: 0.196 ± 0.014
1.368TyrAsp: 1.368 ± 0.042
0.987TyrGlu: 0.987 ± 0.035
0.778TyrPhe: 0.778 ± 0.031
2.037TyrGly: 2.037 ± 0.052
0.454TyrHis: 0.454 ± 0.02
0.652TyrIle: 0.652 ± 0.027
0.541TyrLys: 0.541 ± 0.028
2.025TyrLeu: 2.025 ± 0.051
0.351TyrMet: 0.351 ± 0.019
0.58TyrAsn: 0.58 ± 0.031
1.013TyrPro: 1.013 ± 0.035
0.698TyrGln: 0.698 ± 0.031
1.946TyrArg: 1.946 ± 0.047
1.204TyrSer: 1.204 ± 0.041
0.896TyrThr: 0.896 ± 0.031
1.484TyrVal: 1.484 ± 0.042
0.362TyrTrp: 0.362 ± 0.023
0.555TyrTyr: 0.555 ± 0.027
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 3013 proteins (967107 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski