Streptomyces phage JustBecause

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; unclassified Siphoviridae

Average proteome isoelectric point is 6.11

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 325 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A386K9N9|A0A386K9N9_9CAUD Uncharacterized protein OS=Streptomyces phage JustBecause OX=2315618 GN=327 PE=4 SV=1
MM1 pKa = 7.41TEE3 pKa = 4.94IITLEE8 pKa = 4.19NGDD11 pKa = 3.92QVEE14 pKa = 4.31AGCWLDD20 pKa = 5.27GAAGWTNSYY29 pKa = 10.35RR30 pKa = 11.84IVDD33 pKa = 3.7IATHH37 pKa = 6.46HH38 pKa = 5.94GMEE41 pKa = 4.89LNAEE45 pKa = 4.01DD46 pKa = 3.69TAIVEE51 pKa = 4.65WYY53 pKa = 9.66RR54 pKa = 11.84NSGEE58 pKa = 4.09SDD60 pKa = 2.82AGASDD65 pKa = 5.03DD66 pKa = 4.35EE67 pKa = 4.63LNKK70 pKa = 10.56LEE72 pKa = 4.9AMTGQGGITDD82 pKa = 3.75KK83 pKa = 11.15AIEE86 pKa = 4.15YY87 pKa = 10.31LGEE90 pKa = 3.91QLPEE94 pKa = 3.8GWVLNVEE101 pKa = 3.97MGEE104 pKa = 4.26YY105 pKa = 10.53VVMRR109 pKa = 11.84DD110 pKa = 3.08WVEE113 pKa = 4.36CSAEE117 pKa = 4.18GNGCEE122 pKa = 4.61VDD124 pKa = 3.13TDD126 pKa = 3.67AAGNIVLVKK135 pKa = 10.15RR136 pKa = 11.84CADD139 pKa = 3.45HH140 pKa = 7.01NPCEE144 pKa = 4.2GHH146 pKa = 6.27NGEE149 pKa = 5.49DD150 pKa = 3.83ADD152 pKa = 4.91LMNPGVGMGEE162 pKa = 3.93TTYY165 pKa = 11.31CDD167 pKa = 4.28GSCKK171 pKa = 10.5GG172 pKa = 3.61

Molecular weight: 18.47 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A386KAB5|A0A386KAB5_9CAUD Uncharacterized protein OS=Streptomyces phage JustBecause OX=2315618 GN=115 PE=4 SV=1
MM1 pKa = 7.46YY2 pKa = 10.39DD3 pKa = 2.9HH4 pKa = 7.39AEE6 pKa = 3.74AARR9 pKa = 11.84RR10 pKa = 11.84RR11 pKa = 11.84IVMRR15 pKa = 11.84AAEE18 pKa = 4.26GGILGRR24 pKa = 11.84KK25 pKa = 8.03YY26 pKa = 11.05VEE28 pKa = 4.02LANMLMSGPDD38 pKa = 3.24SPLRR42 pKa = 11.84TSALKK47 pKa = 10.6KK48 pKa = 10.6LADD51 pKa = 3.75SCDD54 pKa = 3.39NAIALAEE61 pKa = 4.13RR62 pKa = 11.84VRR64 pKa = 11.84QSRR67 pKa = 11.84GNSSRR72 pKa = 11.84NPRR75 pKa = 11.84SPGQTNGG82 pKa = 3.1

Molecular weight: 8.91 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

325

0

325

53830

28

1778

165.6

18.23

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

10.134 ± 0.29

1.131 ± 0.082

6.205 ± 0.122

7.468 ± 0.241

2.852 ± 0.084

8.809 ± 0.195

2.127 ± 0.095

4.193 ± 0.099

4.096 ± 0.164

8.057 ± 0.143

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.647 ± 0.083

2.803 ± 0.146

5.322 ± 0.145

3.112 ± 0.111

7.396 ± 0.224

5.8 ± 0.187

5.88 ± 0.157

7.5 ± 0.158

1.861 ± 0.078

2.608 ± 0.09
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski