Amino acid dipepetide frequency for Streptomyces phage JustBecause

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
12.41AlaAla: 12.41 ± 0.804
1.189AlaCys: 1.189 ± 0.134
6.502AlaAsp: 6.502 ± 0.366
7.84AlaGlu: 7.84 ± 0.498
3.028AlaPhe: 3.028 ± 0.281
7.802AlaGly: 7.802 ± 0.431
1.951AlaHis: 1.951 ± 0.217
3.901AlaIle: 3.901 ± 0.398
3.66AlaLys: 3.66 ± 0.359
8.713AlaLeu: 8.713 ± 0.514
3.065AlaMet: 3.065 ± 0.241
2.471AlaAsn: 2.471 ± 0.297
4.329AlaPro: 4.329 ± 0.381
3.604AlaGln: 3.604 ± 0.481
7.561AlaArg: 7.561 ± 0.389
5.332AlaSer: 5.332 ± 0.424
5.387AlaThr: 5.387 ± 0.622
8.007AlaVal: 8.007 ± 0.414
2.025AlaTrp: 2.025 ± 0.199
2.564AlaTyr: 2.564 ± 0.201
0.0AlaXaa: 0.0 ± 0.0
Cys
0.929CysAla: 0.929 ± 0.182
0.242CysCys: 0.242 ± 0.066
0.725CysAsp: 0.725 ± 0.092
0.855CysGlu: 0.855 ± 0.13
0.204CysPhe: 0.204 ± 0.07
1.523CysGly: 1.523 ± 0.183
0.242CysHis: 0.242 ± 0.07
0.26CysIle: 0.26 ± 0.071
0.446CysLys: 0.446 ± 0.103
0.687CysLeu: 0.687 ± 0.117
0.149CysMet: 0.149 ± 0.056
0.26CysAsn: 0.26 ± 0.072
0.966CysPro: 0.966 ± 0.174
0.186CysGln: 0.186 ± 0.061
0.947CysArg: 0.947 ± 0.132
0.632CysSer: 0.632 ± 0.105
0.836CysThr: 0.836 ± 0.137
0.78CysVal: 0.78 ± 0.153
0.204CysTrp: 0.204 ± 0.062
0.242CysTyr: 0.242 ± 0.075
0.0CysXaa: 0.0 ± 0.0
Asp
6.038AspAla: 6.038 ± 0.351
0.669AspCys: 0.669 ± 0.122
3.994AspAsp: 3.994 ± 0.328
4.644AspGlu: 4.644 ± 0.354
2.006AspPhe: 2.006 ± 0.188
6.911AspGly: 6.911 ± 0.484
1.616AspHis: 1.616 ± 0.159
2.471AspIle: 2.471 ± 0.23
1.839AspLys: 1.839 ± 0.199
5.87AspLeu: 5.87 ± 0.325
1.765AspMet: 1.765 ± 0.198
1.133AspAsn: 1.133 ± 0.147
4.013AspPro: 4.013 ± 0.305
1.691AspGln: 1.691 ± 0.183
3.957AspArg: 3.957 ± 0.252
2.954AspSer: 2.954 ± 0.291
2.545AspThr: 2.545 ± 0.225
4.886AspVal: 4.886 ± 0.295
1.598AspTrp: 1.598 ± 0.16
1.449AspTyr: 1.449 ± 0.173
0.0AspXaa: 0.0 ± 0.0
Glu
7.189GluAla: 7.189 ± 0.405
0.687GluCys: 0.687 ± 0.113
4.068GluAsp: 4.068 ± 0.302
5.908GluGlu: 5.908 ± 0.442
2.192GluPhe: 2.192 ± 0.201
5.833GluGly: 5.833 ± 0.384
1.672GluHis: 1.672 ± 0.213
3.734GluIle: 3.734 ± 0.269
3.678GluLys: 3.678 ± 0.297
6.093GluLeu: 6.093 ± 0.412
2.174GluMet: 2.174 ± 0.255
2.396GluAsn: 2.396 ± 0.184
3.846GluPro: 3.846 ± 0.381
3.195GluGln: 3.195 ± 0.256
6.0GluArg: 6.0 ± 0.414
3.79GluSer: 3.79 ± 0.291
3.493GluThr: 3.493 ± 0.262
5.257GluVal: 5.257 ± 0.331
1.356GluTrp: 1.356 ± 0.163
2.118GluTyr: 2.118 ± 0.245
0.0GluXaa: 0.0 ± 0.0
Phe
2.322PheAla: 2.322 ± 0.267
0.409PheCys: 0.409 ± 0.082
2.248PheAsp: 2.248 ± 0.179
1.969PheGlu: 1.969 ± 0.169
0.873PhePhe: 0.873 ± 0.143
2.749PheGly: 2.749 ± 0.269
0.669PheHis: 0.669 ± 0.119
0.91PheIle: 0.91 ± 0.112
0.725PheLys: 0.725 ± 0.145
2.378PheLeu: 2.378 ± 0.253
0.539PheMet: 0.539 ± 0.104
0.78PheAsn: 0.78 ± 0.134
1.709PhePro: 1.709 ± 0.172
1.133PheGln: 1.133 ± 0.168
2.099PheArg: 2.099 ± 0.197
1.858PheSer: 1.858 ± 0.167
2.044PheThr: 2.044 ± 0.215
1.913PheVal: 1.913 ± 0.185
0.557PheTrp: 0.557 ± 0.098
0.632PheTyr: 0.632 ± 0.117
0.0PheXaa: 0.0 ± 0.0
Gly
7.895GlyAla: 7.895 ± 0.548
1.077GlyCys: 1.077 ± 0.13
5.573GlyAsp: 5.573 ± 0.306
6.168GlyGlu: 6.168 ± 0.394
3.01GlyPhe: 3.01 ± 0.225
6.985GlyGly: 6.985 ± 0.522
2.006GlyHis: 2.006 ± 0.174
3.715GlyIle: 3.715 ± 0.251
4.217GlyLys: 4.217 ± 0.34
6.576GlyLeu: 6.576 ± 0.364
2.731GlyMet: 2.731 ± 0.197
2.304GlyAsn: 2.304 ± 0.288
4.124GlyPro: 4.124 ± 0.3
2.879GlyGln: 2.879 ± 0.221
6.353GlyArg: 6.353 ± 0.366
6.279GlySer: 6.279 ± 0.451
5.443GlyThr: 5.443 ± 0.354
6.242GlyVal: 6.242 ± 0.4
2.081GlyTrp: 2.081 ± 0.2
2.415GlyTyr: 2.415 ± 0.225
0.0GlyXaa: 0.0 ± 0.0
His
1.783HisAla: 1.783 ± 0.189
0.279HisCys: 0.279 ± 0.077
1.17HisAsp: 1.17 ± 0.161
1.691HisGlu: 1.691 ± 0.207
0.613HisPhe: 0.613 ± 0.105
1.839HisGly: 1.839 ± 0.2
0.594HisHis: 0.594 ± 0.117
0.725HisIle: 0.725 ± 0.112
0.576HisLys: 0.576 ± 0.113
2.359HisLeu: 2.359 ± 0.237
0.483HisMet: 0.483 ± 0.098
0.669HisAsn: 0.669 ± 0.123
1.523HisPro: 1.523 ± 0.183
0.539HisGln: 0.539 ± 0.106
1.653HisArg: 1.653 ± 0.197
1.096HisSer: 1.096 ± 0.128
1.282HisThr: 1.282 ± 0.144
1.579HisVal: 1.579 ± 0.151
0.279HisTrp: 0.279 ± 0.076
0.539HisTyr: 0.539 ± 0.102
0.0HisXaa: 0.0 ± 0.0
Ile
4.143IleAla: 4.143 ± 0.34
0.39IleCys: 0.39 ± 0.084
3.102IleAsp: 3.102 ± 0.238
3.232IleGlu: 3.232 ± 0.272
1.04IlePhe: 1.04 ± 0.154
3.753IleGly: 3.753 ± 0.235
0.91IleHis: 0.91 ± 0.123
1.208IleIle: 1.208 ± 0.144
1.189IleLys: 1.189 ± 0.163
3.493IleLeu: 3.493 ± 0.273
0.706IleMet: 0.706 ± 0.115
1.263IleAsn: 1.263 ± 0.209
2.545IlePro: 2.545 ± 0.262
1.338IleGln: 1.338 ± 0.13
3.585IleArg: 3.585 ± 0.271
2.415IleSer: 2.415 ± 0.258
2.917IleThr: 2.917 ± 0.263
3.047IleVal: 3.047 ± 0.214
0.594IleTrp: 0.594 ± 0.116
1.059IleTyr: 1.059 ± 0.118
0.0IleXaa: 0.0 ± 0.0
Lys
5.016LysAla: 5.016 ± 0.37
0.39LysCys: 0.39 ± 0.092
2.118LysAsp: 2.118 ± 0.229
2.434LysGlu: 2.434 ± 0.213
1.115LysPhe: 1.115 ± 0.154
3.697LysGly: 3.697 ± 0.294
0.892LysHis: 0.892 ± 0.125
2.304LysIle: 2.304 ± 0.176
2.434LysLys: 2.434 ± 0.233
3.437LysLeu: 3.437 ± 0.399
1.059LysMet: 1.059 ± 0.125
1.338LysAsn: 1.338 ± 0.164
2.304LysPro: 2.304 ± 0.242
0.873LysGln: 0.873 ± 0.152
2.842LysArg: 2.842 ± 0.273
1.783LysSer: 1.783 ± 0.209
2.229LysThr: 2.229 ± 0.202
3.214LysVal: 3.214 ± 0.253
0.65LysTrp: 0.65 ± 0.102
0.836LysTyr: 0.836 ± 0.119
0.0LysXaa: 0.0 ± 0.0
Leu
9.196LeuAla: 9.196 ± 0.593
1.077LeuCys: 1.077 ± 0.149
5.666LeuAsp: 5.666 ± 0.308
5.74LeuGlu: 5.74 ± 0.432
1.765LeuPhe: 1.765 ± 0.206
5.963LeuGly: 5.963 ± 0.337
1.523LeuHis: 1.523 ± 0.197
3.195LeuIle: 3.195 ± 0.217
4.198LeuLys: 4.198 ± 0.279
6.762LeuLeu: 6.762 ± 0.43
2.118LeuMet: 2.118 ± 0.206
2.619LeuAsn: 2.619 ± 0.212
4.124LeuPro: 4.124 ± 0.272
1.56LeuGln: 1.56 ± 0.206
5.796LeuArg: 5.796 ± 0.308
4.849LeuSer: 4.849 ± 0.385
5.332LeuThr: 5.332 ± 0.292
5.666LeuVal: 5.666 ± 0.336
1.3LeuTrp: 1.3 ± 0.192
2.118LeuTyr: 2.118 ± 0.2
0.0LeuXaa: 0.0 ± 0.0
Met
2.972MetAla: 2.972 ± 0.234
0.149MetCys: 0.149 ± 0.047
1.56MetAsp: 1.56 ± 0.165
1.579MetGlu: 1.579 ± 0.204
0.502MetPhe: 0.502 ± 0.104
2.322MetGly: 2.322 ± 0.211
0.613MetHis: 0.613 ± 0.104
1.003MetIle: 1.003 ± 0.13
1.56MetLys: 1.56 ± 0.204
1.746MetLeu: 1.746 ± 0.192
0.576MetMet: 0.576 ± 0.112
1.3MetAsn: 1.3 ± 0.15
1.338MetPro: 1.338 ± 0.157
0.892MetGln: 0.892 ± 0.133
2.266MetArg: 2.266 ± 0.213
1.932MetSer: 1.932 ± 0.174
2.006MetThr: 2.006 ± 0.189
1.412MetVal: 1.412 ± 0.162
0.334MetTrp: 0.334 ± 0.072
0.409MetTyr: 0.409 ± 0.091
0.0MetXaa: 0.0 ± 0.0
Asn
2.917AsnAla: 2.917 ± 0.329
0.297AsnCys: 0.297 ± 0.071
1.43AsnAsp: 1.43 ± 0.144
1.505AsnGlu: 1.505 ± 0.157
0.687AsnPhe: 0.687 ± 0.132
3.678AsnGly: 3.678 ± 0.41
0.539AsnHis: 0.539 ± 0.106
1.059AsnIle: 1.059 ± 0.15
1.059AsnLys: 1.059 ± 0.176
2.489AsnLeu: 2.489 ± 0.278
0.446AsnMet: 0.446 ± 0.101
0.799AsnAsn: 0.799 ± 0.146
1.951AsnPro: 1.951 ± 0.17
0.725AsnGln: 0.725 ± 0.12
1.988AsnArg: 1.988 ± 0.203
1.895AsnSer: 1.895 ± 0.207
1.412AsnThr: 1.412 ± 0.211
1.988AsnVal: 1.988 ± 0.187
0.464AsnTrp: 0.464 ± 0.108
0.706AsnTyr: 0.706 ± 0.111
0.0AsnXaa: 0.0 ± 0.0
Pro
5.443ProAla: 5.443 ± 0.339
0.817ProCys: 0.817 ± 0.148
3.734ProAsp: 3.734 ± 0.284
5.536ProGlu: 5.536 ± 0.368
1.3ProPhe: 1.3 ± 0.167
5.666ProGly: 5.666 ± 0.356
0.929ProHis: 0.929 ± 0.122
1.579ProIle: 1.579 ± 0.16
1.969ProLys: 1.969 ± 0.212
3.715ProLeu: 3.715 ± 0.249
0.836ProMet: 0.836 ± 0.126
1.3ProAsn: 1.3 ± 0.161
2.582ProPro: 2.582 ± 0.274
1.56ProGln: 1.56 ± 0.2
3.158ProArg: 3.158 ± 0.288
2.972ProSer: 2.972 ± 0.241
2.768ProThr: 2.768 ± 0.261
4.904ProVal: 4.904 ± 0.409
0.929ProTrp: 0.929 ± 0.15
1.523ProTyr: 1.523 ± 0.166
0.0ProXaa: 0.0 ± 0.0
Gln
3.53GlnAla: 3.53 ± 0.392
0.334GlnCys: 0.334 ± 0.086
1.486GlnAsp: 1.486 ± 0.169
2.842GlnGlu: 2.842 ± 0.257
0.855GlnPhe: 0.855 ± 0.118
2.582GlnGly: 2.582 ± 0.239
0.65GlnHis: 0.65 ± 0.11
2.062GlnIle: 2.062 ± 0.196
1.505GlnLys: 1.505 ± 0.207
1.913GlnLeu: 1.913 ± 0.242
0.762GlnMet: 0.762 ± 0.126
0.836GlnAsn: 0.836 ± 0.139
1.505GlnPro: 1.505 ± 0.164
1.338GlnGln: 1.338 ± 0.177
1.988GlnArg: 1.988 ± 0.196
1.505GlnSer: 1.505 ± 0.175
1.523GlnThr: 1.523 ± 0.192
2.341GlnVal: 2.341 ± 0.179
0.576GlnTrp: 0.576 ± 0.109
0.985GlnTyr: 0.985 ± 0.11
0.0GlnXaa: 0.0 ± 0.0
Arg
6.446ArgAla: 6.446 ± 0.418
0.892ArgCys: 0.892 ± 0.156
4.329ArgAsp: 4.329 ± 0.338
6.205ArgGlu: 6.205 ± 0.425
2.099ArgPhe: 2.099 ± 0.204
5.109ArgGly: 5.109 ± 0.337
1.876ArgHis: 1.876 ± 0.211
4.068ArgIle: 4.068 ± 0.279
3.678ArgLys: 3.678 ± 0.342
5.499ArgLeu: 5.499 ± 0.327
2.712ArgMet: 2.712 ± 0.294
2.192ArgAsn: 2.192 ± 0.223
3.697ArgPro: 3.697 ± 0.326
2.415ArgGln: 2.415 ± 0.196
6.149ArgArg: 6.149 ± 0.422
4.198ArgSer: 4.198 ± 0.268
4.236ArgThr: 4.236 ± 0.261
5.053ArgVal: 5.053 ± 0.314
1.3ArgTrp: 1.3 ± 0.178
1.802ArgTyr: 1.802 ± 0.208
0.0ArgXaa: 0.0 ± 0.0
Ser
5.889SerAla: 5.889 ± 0.468
0.632SerCys: 0.632 ± 0.106
3.585SerAsp: 3.585 ± 0.287
3.938SerGlu: 3.938 ± 0.29
1.765SerPhe: 1.765 ± 0.172
6.632SerGly: 6.632 ± 0.518
0.855SerHis: 0.855 ± 0.124
1.988SerIle: 1.988 ± 0.175
1.951SerLys: 1.951 ± 0.233
4.347SerLeu: 4.347 ± 0.306
2.006SerMet: 2.006 ± 0.187
1.375SerAsn: 1.375 ± 0.216
2.582SerPro: 2.582 ± 0.252
1.783SerGln: 1.783 ± 0.188
3.846SerArg: 3.846 ± 0.313
3.901SerSer: 3.901 ± 0.533
3.344SerThr: 3.344 ± 0.26
4.849SerVal: 4.849 ± 0.401
0.947SerTrp: 0.947 ± 0.123
1.783SerTyr: 1.783 ± 0.222
0.0SerXaa: 0.0 ± 0.0
Thr
5.889ThrAla: 5.889 ± 0.477
0.706ThrCys: 0.706 ± 0.141
3.53ThrAsp: 3.53 ± 0.263
4.57ThrGlu: 4.57 ± 0.321
1.932ThrPhe: 1.932 ± 0.216
6.168ThrGly: 6.168 ± 0.469
1.077ThrHis: 1.077 ± 0.152
2.359ThrIle: 2.359 ± 0.235
1.783ThrLys: 1.783 ± 0.227
4.459ThrLeu: 4.459 ± 0.289
1.3ThrMet: 1.3 ± 0.145
1.245ThrAsn: 1.245 ± 0.16
3.567ThrPro: 3.567 ± 0.274
1.821ThrGln: 1.821 ± 0.219
3.79ThrArg: 3.79 ± 0.253
3.307ThrSer: 3.307 ± 0.26
3.604ThrThr: 3.604 ± 0.338
5.053ThrVal: 5.053 ± 0.282
1.115ThrTrp: 1.115 ± 0.16
1.523ThrTyr: 1.523 ± 0.189
0.0ThrXaa: 0.0 ± 0.0
Val
7.097ValAla: 7.097 ± 0.411
0.669ValCys: 0.669 ± 0.135
4.217ValAsp: 4.217 ± 0.293
5.183ValGlu: 5.183 ± 0.343
2.434ValPhe: 2.434 ± 0.207
4.719ValGly: 4.719 ± 0.35
1.802ValHis: 1.802 ± 0.192
3.548ValIle: 3.548 ± 0.232
3.121ValLys: 3.121 ± 0.273
6.186ValLeu: 6.186 ± 0.374
1.783ValMet: 1.783 ± 0.171
2.322ValAsn: 2.322 ± 0.241
4.273ValPro: 4.273 ± 0.302
2.396ValGln: 2.396 ± 0.225
5.778ValArg: 5.778 ± 0.339
4.7ValSer: 4.7 ± 0.253
5.666ValThr: 5.666 ± 0.381
5.443ValVal: 5.443 ± 0.365
1.412ValTrp: 1.412 ± 0.177
2.248ValTyr: 2.248 ± 0.225
0.0ValXaa: 0.0 ± 0.0
Trp
1.523TrpAla: 1.523 ± 0.195
0.167TrpCys: 0.167 ± 0.05
1.208TrpAsp: 1.208 ± 0.155
0.985TrpGlu: 0.985 ± 0.151
0.539TrpPhe: 0.539 ± 0.085
1.393TrpGly: 1.393 ± 0.159
0.297TrpHis: 0.297 ± 0.071
0.873TrpIle: 0.873 ± 0.138
0.687TrpLys: 0.687 ± 0.112
1.709TrpLeu: 1.709 ± 0.168
0.632TrpMet: 0.632 ± 0.097
0.632TrpAsn: 0.632 ± 0.113
0.799TrpPro: 0.799 ± 0.138
0.613TrpGln: 0.613 ± 0.103
1.728TrpArg: 1.728 ± 0.16
1.189TrpSer: 1.189 ± 0.143
1.393TrpThr: 1.393 ± 0.187
1.375TrpVal: 1.375 ± 0.182
0.39TrpTrp: 0.39 ± 0.089
0.483TrpTyr: 0.483 ± 0.094
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.712TyrAla: 2.712 ± 0.255
0.242TyrCys: 0.242 ± 0.07
1.802TyrAsp: 1.802 ± 0.193
1.858TyrGlu: 1.858 ± 0.213
0.557TyrPhe: 0.557 ± 0.092
2.768TyrGly: 2.768 ± 0.284
0.557TyrHis: 0.557 ± 0.099
0.966TyrIle: 0.966 ± 0.126
0.687TyrLys: 0.687 ± 0.117
2.136TyrLeu: 2.136 ± 0.191
0.613TyrMet: 0.613 ± 0.115
0.799TyrAsn: 0.799 ± 0.12
1.449TyrPro: 1.449 ± 0.158
0.576TyrGln: 0.576 ± 0.113
2.471TyrArg: 2.471 ± 0.218
1.412TyrSer: 1.412 ± 0.145
1.338TyrThr: 1.338 ± 0.156
1.988TyrVal: 1.988 ± 0.186
0.502TyrTrp: 0.502 ± 0.099
0.65TyrTyr: 0.65 ± 0.114
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 325 proteins (53830 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski