Desulfovibrio carbinoliphilus subsp. oakridgensis
Average proteome isoelectric point is 6.7
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 3713 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|G7Q8V4|G7Q8V4_9DELT Uncharacterized protein OS=Desulfovibrio carbinoliphilus subsp. oakridgensis OX=694327 GN=DFW101_1432 PE=4 SV=1
MM1 pKa = 8.02 ADD3 pKa = 3.16 TGNPGTFLLFSDD15 pKa = 3.53 VHH17 pKa = 6.86 FDD19 pKa = 3.72 PFADD23 pKa = 4.12 PSLVPALAASDD34 pKa = 3.7 VSAWTGLLSSSSQTAYY50 pKa = 10.43 AAYY53 pKa = 9.88 GSDD56 pKa = 3.03 SNYY59 pKa = 10.4 PLFEE63 pKa = 4.36 SALDD67 pKa = 3.79 DD68 pKa = 3.75 MAARR72 pKa = 11.84 AADD75 pKa = 3.74 VSTILYY81 pKa = 9.46 PGDD84 pKa = 3.33 ILAHH88 pKa = 6.89 DD89 pKa = 5.06 FPQDD93 pKa = 3.51 YY94 pKa = 10.63 AALTGDD100 pKa = 4.05 TSQAGLDD107 pKa = 3.39 AFAQKK112 pKa = 9.06 TVAFFVQEE120 pKa = 3.88 VDD122 pKa = 3.77 SRR124 pKa = 11.84 FPDD127 pKa = 3.49 AMVLVADD134 pKa = 5.27 GNCDD138 pKa = 3.4 TDD140 pKa = 3.71 NMSGSIGARR149 pKa = 11.84 PGDD152 pKa = 4.12 PYY154 pKa = 11.45 LSDD157 pKa = 3.69 TAPLLAQAFFNNDD170 pKa = 2.82 TDD172 pKa = 3.68 RR173 pKa = 11.84 TAFASTYY180 pKa = 11.02 AVGGYY185 pKa = 9.53 YY186 pKa = 10.23 AVEE189 pKa = 4.22 PDD191 pKa = 3.73 GPTGLKK197 pKa = 10.61 YY198 pKa = 10.26 IVLNDD203 pKa = 3.61 NLWITEE209 pKa = 3.97 YY210 pKa = 11.08 DD211 pKa = 3.75 DD212 pKa = 4.17 PAAGAVEE219 pKa = 4.26 LSWFASEE226 pKa = 4.9 LAEE229 pKa = 4.91 SAQNFQKK236 pKa = 10.5 VWVVGHH242 pKa = 6.41 IPVGANASSVVASYY256 pKa = 11.12 DD257 pKa = 3.16 QTGRR261 pKa = 11.84 IAYY264 pKa = 9.8 SGNLDD269 pKa = 3.88 DD270 pKa = 5.45 GFNAAFVGLEE280 pKa = 3.92 LAYY283 pKa = 10.59 DD284 pKa = 3.77 ATIKK288 pKa = 10.24 ATFTGHH294 pKa = 4.54 THH296 pKa = 6.55 NDD298 pKa = 3.4 DD299 pKa = 3.46 FRR301 pKa = 11.84 LVTDD305 pKa = 4.54 GSGAANLLRR314 pKa = 11.84 IAPSISPVFDD324 pKa = 3.94 NNPGYY329 pKa = 10.14 QVYY332 pKa = 10.7 SFDD335 pKa = 3.73 TRR337 pKa = 11.84 TSSLLDD343 pKa = 3.29 EE344 pKa = 4.46 TTYY347 pKa = 11.15 TLDD350 pKa = 3.88 LQSSSPAWSKK360 pKa = 10.51 EE361 pKa = 3.5 YY362 pKa = 10.56 AYY364 pKa = 10.63 AEE366 pKa = 4.55 TYY368 pKa = 10.15 GQTLATPQEE377 pKa = 3.91 WQAVYY382 pKa = 10.29 GAILTNPATQAAYY395 pKa = 9.42 IDD397 pKa = 4.18 HH398 pKa = 7.36 LGQGAATQFPVTAANLPAYY417 pKa = 9.78 LATPGFVLPATYY429 pKa = 10.29 NAAVAALAGG438 pKa = 3.81
Molecular weight: 46.31 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.799
IPC2_protein 3.706
IPC_protein 3.757
Toseland 3.516
ProMoST 3.935
Dawson 3.77
Bjellqvist 3.923
Wikipedia 3.732
Rodwell 3.579
Grimsley 3.427
Solomon 3.757
Lehninger 3.719
Nozaki 3.872
DTASelect 4.177
Thurlkill 3.579
EMBOSS 3.732
Sillero 3.884
Patrickios 0.261
IPC_peptide 3.745
IPC2_peptide 3.859
IPC2.peptide.svr19 3.783
Protein with the highest isoelectric point:
>tr|G7Q6L8|G7Q6L8_9DELT NADH dehydrogenase (Quinone) OS=Desulfovibrio carbinoliphilus subsp. oakridgensis OX=694327 GN=DFW101_1623 PE=4 SV=1
MM1 pKa = 7.43 SKK3 pKa = 8.62 KK4 pKa = 8.87 TYY6 pKa = 9.67 QPSKK10 pKa = 9.23 IRR12 pKa = 11.84 RR13 pKa = 11.84 KK14 pKa = 8.13 RR15 pKa = 11.84 THH17 pKa = 5.96 GFLVRR22 pKa = 11.84 SRR24 pKa = 11.84 TKK26 pKa = 10.32 NGQAILRR33 pKa = 11.84 RR34 pKa = 11.84 RR35 pKa = 11.84 RR36 pKa = 11.84 AKK38 pKa = 9.7 GRR40 pKa = 11.84 KK41 pKa = 8.66 RR42 pKa = 11.84 LAVV45 pKa = 3.41
Molecular weight: 5.39 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.433
IPC2_protein 11.125
IPC_protein 12.501
Toseland 12.661
ProMoST 13.159
Dawson 12.661
Bjellqvist 12.661
Wikipedia 13.144
Rodwell 12.398
Grimsley 12.705
Solomon 13.159
Lehninger 13.056
Nozaki 12.661
DTASelect 12.661
Thurlkill 12.661
EMBOSS 13.159
Sillero 12.661
Patrickios 12.135
IPC_peptide 13.159
IPC2_peptide 12.149
IPC2.peptide.svr19 9.06
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
3713
0
3713
1225608
30
2352
330.1
35.7
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
12.929 ± 0.076
1.281 ± 0.017
5.48 ± 0.027
5.761 ± 0.041
3.872 ± 0.026
8.783 ± 0.042
1.871 ± 0.017
4.223 ± 0.035
3.668 ± 0.034
11.123 ± 0.048
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.327 ± 0.016
2.33 ± 0.023
5.645 ± 0.039
2.746 ± 0.024
7.162 ± 0.041
4.823 ± 0.03
5.032 ± 0.035
7.477 ± 0.033
1.108 ± 0.015
2.359 ± 0.023
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here