Lactococcus virus 712

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Skunavirus

Average proteome isoelectric point is 6.35

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 55 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|Q09WU6|Q09WU6_9CAUD Putative structural protein OS=Lactococcus virus 712 OX=213769 PE=4 SV=1
MM1 pKa = 7.52EE2 pKa = 5.73FDD4 pKa = 6.24SYY6 pKa = 11.4MDD8 pKa = 3.4WLNNLLTMPLNDD20 pKa = 3.86VILGVKK26 pKa = 8.96DD27 pKa = 3.57TIEE30 pKa = 4.55DD31 pKa = 3.52KK32 pKa = 10.66TVYY35 pKa = 10.62LSLSDD40 pKa = 3.67SKK42 pKa = 11.01VIKK45 pKa = 9.72MDD47 pKa = 3.07NTSFVMGYY55 pKa = 9.13YY56 pKa = 8.74YY57 pKa = 10.45QVVLSVKK64 pKa = 10.52DD65 pKa = 3.51VDD67 pKa = 4.41DD68 pKa = 4.44EE69 pKa = 4.52LVGLVGDD76 pKa = 4.15VLQNGWNMTNWSEE89 pKa = 4.32NSHH92 pKa = 6.58LYY94 pKa = 10.7NYY96 pKa = 8.61TGTVYY101 pKa = 10.42LPCGAGGQAWQQ112 pKa = 3.59

Molecular weight: 12.71 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|Q09WU5|Q09WU5_9CAUD Putative structural protein OS=Lactococcus virus 712 OX=213769 PE=4 SV=1
MM1 pKa = 6.54WCRR4 pKa = 11.84WSSMAMNLLNTSNIAKK20 pKa = 9.93EE21 pKa = 4.06MQTKK25 pKa = 7.47VTEE28 pKa = 4.24RR29 pKa = 11.84MGDD32 pKa = 2.9WFEE35 pKa = 5.71AEE37 pKa = 4.92FKK39 pKa = 11.02AKK41 pKa = 10.75ANTASRR47 pKa = 11.84RR48 pKa = 11.84TRR50 pKa = 11.84LIRR53 pKa = 11.84SHH55 pKa = 5.25GHH57 pKa = 5.22TYY59 pKa = 9.85TYY61 pKa = 11.35ARR63 pKa = 11.84YY64 pKa = 9.69QNTGQLSGNLKK75 pKa = 9.15QVKK78 pKa = 9.81KK79 pKa = 10.45GDD81 pKa = 4.02KK82 pKa = 9.89IVVNAGTRR90 pKa = 11.84ANYY93 pKa = 10.18ASGYY97 pKa = 8.71HH98 pKa = 5.92GMYY101 pKa = 10.56FLVEE105 pKa = 4.16KK106 pKa = 10.74KK107 pKa = 10.71GMQDD111 pKa = 3.28VKK113 pKa = 9.28TTLKK117 pKa = 10.68KK118 pKa = 10.0GANYY122 pKa = 9.69ANSMKK127 pKa = 10.65LL128 pKa = 3.26

Molecular weight: 14.61 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

55

0

55

8885

36

999

161.5

18.44

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.83 ± 0.587

0.698 ± 0.17

6.021 ± 0.263

7.53 ± 0.616

4.423 ± 0.259

6.1 ± 0.561

1.261 ± 0.184

6.888 ± 0.295

9.229 ± 0.478

8.542 ± 0.417

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.6 ± 0.175

6.449 ± 0.278

2.183 ± 0.264

3.376 ± 0.246

3.59 ± 0.245

6.337 ± 0.408

6.427 ± 0.303

6.494 ± 0.367

1.711 ± 0.167

4.311 ± 0.421
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski