Ruminococcus callidus ATCC 27760

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Clostridia; Eubacteriales; Oscillospiraceae; Ruminococcus; Ruminococcus callidus

Average proteome isoelectric point is 6.39

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3475 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|U2JPD7|U2JPD7_9FIRM Glutamine amidotransferase type-2 domain-containing protein (Fragment) OS=Ruminococcus callidus ATCC 27760 OX=411473 GN=RUMCAL_03165 PE=4 SV=1
TT1 pKa = 6.4TATTATTTTTAPTAAKK17 pKa = 10.21GDD19 pKa = 3.69ASGDD23 pKa = 3.71GVLDD27 pKa = 3.76TNDD30 pKa = 3.0VFEE33 pKa = 5.88AMLCVAYY40 pKa = 10.21RR41 pKa = 11.84GAGMSSNLTADD52 pKa = 3.73QIAAADD58 pKa = 3.58IDD60 pKa = 4.19GDD62 pKa = 4.18GSVDD66 pKa = 3.6STDD69 pKa = 2.89VYY71 pKa = 11.3YY72 pKa = 10.25ILYY75 pKa = 10.08YY76 pKa = 10.6VALQGAGKK84 pKa = 10.04NPTWDD89 pKa = 3.39FVLGRR94 pKa = 11.84KK95 pKa = 8.46

Molecular weight: 9.76 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|U2LJ93|U2LJ93_9FIRM Kinase PfkB family OS=Ruminococcus callidus ATCC 27760 OX=411473 GN=RUMCAL_03397 PE=4 SV=1
MM1 pKa = 7.38HH2 pKa = 8.1RR3 pKa = 11.84LPQQQLRR10 pKa = 11.84QQRR13 pKa = 11.84PPPRR17 pKa = 11.84QQLRR21 pKa = 11.84LQTKK25 pKa = 9.56RR26 pKa = 11.84LQQLRR31 pKa = 11.84QPLLQAKK38 pKa = 9.76RR39 pKa = 11.84PQQPRR44 pKa = 11.84QLLPQTKK51 pKa = 9.23QPQQLRR57 pKa = 11.84QLLQTKK63 pKa = 8.33QLQQPRR69 pKa = 11.84KK70 pKa = 9.1QLPQAKK76 pKa = 9.1QLQQPRR82 pKa = 11.84VLLQQAKK89 pKa = 10.09RR90 pKa = 11.84LPQLRR95 pKa = 11.84KK96 pKa = 9.46QLPQMKK102 pKa = 9.73QPQQPKK108 pKa = 10.26KK109 pKa = 10.62LPQQ112 pKa = 3.66

Molecular weight: 13.64 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3475

0

3475

928887

36

7715

267.3

29.85

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.344 ± 0.043

1.875 ± 0.026

5.676 ± 0.052

6.711 ± 0.048

4.106 ± 0.032

6.744 ± 0.044

1.986 ± 0.023

6.289 ± 0.045

5.866 ± 0.047

8.979 ± 0.063

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.856 ± 0.023

4.168 ± 0.046

3.636 ± 0.028

3.989 ± 0.039

4.544 ± 0.046

6.154 ± 0.039

6.342 ± 0.066

6.671 ± 0.037

1.035 ± 0.018

4.029 ± 0.048
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski