Amino acid dipepetide frequency for Ruminococcus callidus ATCC 27760

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	9.618AlaAla: 9.618 ± 0.164	1.31AlaCys: 1.31 ± 0.04	5.426AlaAsp: 5.426 ± 0.1	6.607AlaGlu: 6.607 ± 0.109	3.575AlaPhe: 3.575 ± 0.08	6.135AlaGly: 6.135 ± 0.102	1.177AlaHis: 1.177 ± 0.035	4.843AlaIle: 4.843 ± 0.097	4.75AlaLys: 4.75 ± 0.08	7.205AlaLeu: 7.205 ± 0.108	2.708AlaMet: 2.708 ± 0.06	2.762AlaAsn: 2.762 ± 0.061	2.385AlaPro: 2.385 ± 0.057	2.951AlaGln: 2.951 ± 0.064	2.868AlaArg: 2.868 ± 0.063	4.166AlaSer: 4.166 ± 0.076	3.937AlaThr: 3.937 ± 0.105	7.161AlaVal: 7.161 ± 0.106	0.719AlaTrp: 0.719 ± 0.029	3.132AlaTyr: 3.132 ± 0.056	0.0AlaXaa: 0.0 ± 0.0
Cys	1.482CysAla: 1.482 ± 0.038	0.428CysCys: 0.428 ± 0.022	1.018CysAsp: 1.018 ± 0.036	1.017CysGlu: 1.017 ± 0.036	0.81CysPhe: 0.81 ± 0.032	1.871CysGly: 1.871 ± 0.057	0.386CysHis: 0.386 ± 0.022	1.128CysIle: 1.128 ± 0.039	0.998CysLys: 0.998 ± 0.033	1.359CysLeu: 1.359 ± 0.041	0.473CysMet: 0.473 ± 0.022	0.751CysAsn: 0.751 ± 0.028	0.777CysPro: 0.777 ± 0.03	0.543CysGln: 0.543 ± 0.025	1.077CysArg: 1.077 ± 0.039	1.268CysSer: 1.268 ± 0.049	1.281CysThr: 1.281 ± 0.051	1.212CysVal: 1.212 ± 0.04	0.159CysTrp: 0.159 ± 0.012	0.713CysTyr: 0.713 ± 0.03	0.0CysXaa: 0.0 ± 0.0
Asp	5.064AspAla: 5.064 ± 0.081	1.122AspCys: 1.122 ± 0.032	3.25AspAsp: 3.25 ± 0.089	3.675AspGlu: 3.675 ± 0.079	2.705AspPhe: 2.705 ± 0.059	4.947AspGly: 4.947 ± 0.127	0.934AspHis: 0.934 ± 0.03	3.682AspIle: 3.682 ± 0.068	3.001AspLys: 3.001 ± 0.066	4.509AspLeu: 4.509 ± 0.07	1.725AspMet: 1.725 ± 0.04	2.181AspAsn: 2.181 ± 0.055	1.716AspPro: 1.716 ± 0.049	1.35AspGln: 1.35 ± 0.045	2.17AspArg: 2.17 ± 0.043	3.793AspSer: 3.793 ± 0.084	3.814AspThr: 3.814 ± 0.082	3.885AspVal: 3.885 ± 0.07	0.705AspTrp: 0.705 ± 0.03	2.533AspTyr: 2.533 ± 0.068	0.0AspXaa: 0.0 ± 0.0
Glu	4.956GluAla: 4.956 ± 0.093	0.838GluCys: 0.838 ± 0.033	3.374GluAsp: 3.374 ± 0.074	5.172GluGlu: 5.172 ± 0.113	2.182GluPhe: 2.182 ± 0.055	3.153GluGly: 3.153 ± 0.063	1.41GluHis: 1.41 ± 0.045	4.814GluIle: 4.814 ± 0.08	5.301GluLys: 5.301 ± 0.095	6.014GluLeu: 6.014 ± 0.089	2.146GluMet: 2.146 ± 0.049	3.974GluAsn: 3.974 ± 0.082	2.045GluPro: 2.045 ± 0.055	3.684GluGln: 3.684 ± 0.083	3.11GluArg: 3.11 ± 0.067	3.604GluSer: 3.604 ± 0.063	4.477GluThr: 4.477 ± 0.094	3.859GluVal: 3.859 ± 0.068	0.536GluTrp: 0.536 ± 0.022	2.464GluTyr: 2.464 ± 0.057	0.0GluXaa: 0.0 ± 0.0
Phe	3.248PheAla: 3.248 ± 0.063	1.083PheCys: 1.083 ± 0.036	2.408PheAsp: 2.408 ± 0.061	2.171PheGlu: 2.171 ± 0.049	2.002PhePhe: 2.002 ± 0.058	2.981PheGly: 2.981 ± 0.064	1.02PheHis: 1.02 ± 0.037	1.996PheIle: 1.996 ± 0.059	1.354PheLys: 1.354 ± 0.042	4.119PheLeu: 4.119 ± 0.086	1.018PheMet: 1.018 ± 0.036	1.261PheAsn: 1.261 ± 0.04	1.682PhePro: 1.682 ± 0.051	1.749PheGln: 1.749 ± 0.05	2.265PheArg: 2.265 ± 0.047	3.426PheSer: 3.426 ± 0.071	2.413PheThr: 2.413 ± 0.056	2.564PheVal: 2.564 ± 0.058	0.467PheTrp: 0.467 ± 0.024	1.832PheTyr: 1.832 ± 0.049	0.0PheXaa: 0.0 ± 0.0
Gly	4.754GlyAla: 4.754 ± 0.105	1.454GlyCys: 1.454 ± 0.051	3.808GlyAsp: 3.808 ± 0.084	4.382GlyGlu: 4.382 ± 0.079	2.914GlyPhe: 2.914 ± 0.062	4.752GlyGly: 4.752 ± 0.102	1.122GlyHis: 1.122 ± 0.035	5.261GlyIle: 5.261 ± 0.089	5.218GlyLys: 5.218 ± 0.083	5.262GlyLeu: 5.262 ± 0.094	2.197GlyMet: 2.197 ± 0.053	3.181GlyAsn: 3.181 ± 0.072	0.9GlyPro: 0.9 ± 0.036	1.911GlyGln: 1.911 ± 0.048	2.593GlyArg: 2.593 ± 0.059	4.291GlySer: 4.291 ± 0.079	4.729GlyThr: 4.729 ± 0.086	5.019GlyVal: 5.019 ± 0.084	0.759GlyTrp: 0.759 ± 0.039	2.927GlyTyr: 2.927 ± 0.064	0.0GlyXaa: 0.0 ± 0.0
His	1.514HisAla: 1.514 ± 0.043	0.474HisCys: 0.474 ± 0.025	0.975HisAsp: 0.975 ± 0.037	0.997HisGlu: 0.997 ± 0.034	0.975HisPhe: 0.975 ± 0.034	1.475HisGly: 1.475 ± 0.043	0.504HisHis: 0.504 ± 0.025	1.43HisIle: 1.43 ± 0.042	1.059HisLys: 1.059 ± 0.035	1.794HisLeu: 1.794 ± 0.047	0.61HisMet: 0.61 ± 0.029	0.827HisAsn: 0.827 ± 0.036	0.85HisPro: 0.85 ± 0.03	0.672HisGln: 0.672 ± 0.027	0.99HisArg: 0.99 ± 0.041	1.13HisSer: 1.13 ± 0.032	1.302HisThr: 1.302 ± 0.04	1.197HisVal: 1.197 ± 0.042	0.284HisTrp: 0.284 ± 0.02	0.803HisTyr: 0.803 ± 0.03	0.0HisXaa: 0.0 ± 0.0
Ile	5.699IleAla: 5.699 ± 0.092	1.361IleCys: 1.361 ± 0.041	3.617IleAsp: 3.617 ± 0.072	3.388IleGlu: 3.388 ± 0.073	2.601IlePhe: 2.601 ± 0.06	4.309IleGly: 4.309 ± 0.09	1.264IleHis: 1.264 ± 0.034	3.544IleIle: 3.544 ± 0.091	2.652IleLys: 2.652 ± 0.068	5.977IleLeu: 5.977 ± 0.108	1.506IleMet: 1.506 ± 0.042	2.128IleAsn: 2.128 ± 0.055	3.279IlePro: 3.279 ± 0.066	2.444IleGln: 2.444 ± 0.049	3.297IleArg: 3.297 ± 0.061	4.648IleSer: 4.648 ± 0.088	4.19IleThr: 4.19 ± 0.075	4.045IleVal: 4.045 ± 0.07	0.653IleTrp: 0.653 ± 0.031	2.283IleTyr: 2.283 ± 0.063	0.0IleXaa: 0.0 ± 0.0
Lys	4.602LysAla: 4.602 ± 0.082	0.83LysCys: 0.83 ± 0.035	3.029LysAsp: 3.029 ± 0.069	4.072LysGlu: 4.072 ± 0.075	1.847LysPhe: 1.847 ± 0.049	3.32LysGly: 3.32 ± 0.07	1.161LysHis: 1.161 ± 0.038	3.951LysIle: 3.951 ± 0.082	4.758LysLys: 4.758 ± 0.097	5.163LysLeu: 5.163 ± 0.086	1.826LysMet: 1.826 ± 0.043	3.025LysAsn: 3.025 ± 0.061	2.167LysPro: 2.167 ± 0.049	2.545LysGln: 2.545 ± 0.057	2.934LysArg: 2.934 ± 0.059	3.462LysSer: 3.462 ± 0.077	3.66LysThr: 3.66 ± 0.073	3.284LysVal: 3.284 ± 0.064	0.643LysTrp: 0.643 ± 0.029	2.379LysTyr: 2.379 ± 0.059	0.0LysXaa: 0.0 ± 0.0
Leu	6.786LeuAla: 6.786 ± 0.089	2.164LeuCys: 2.164 ± 0.053	4.938LeuAsp: 4.938 ± 0.069	6.257LeuGlu: 6.257 ± 0.095	3.646LeuPhe: 3.646 ± 0.082	5.374LeuGly: 5.374 ± 0.11	2.161LeuHis: 2.161 ± 0.047	4.776LeuIle: 4.776 ± 0.094	4.79LeuLys: 4.79 ± 0.08	9.129LeuLeu: 9.129 ± 0.184	2.301LeuMet: 2.301 ± 0.055	3.253LeuAsn: 3.253 ± 0.075	4.151LeuPro: 4.151 ± 0.071	3.982LeuGln: 3.982 ± 0.078	4.518LeuArg: 4.518 ± 0.096	6.131LeuSer: 6.131 ± 0.091	5.834LeuThr: 5.834 ± 0.092	5.139LeuVal: 5.139 ± 0.086	0.902LeuTrp: 0.902 ± 0.037	3.556LeuTyr: 3.556 ± 0.065	0.0LeuXaa: 0.0 ± 0.0
Met	2.262MetAla: 2.262 ± 0.055	0.397MetCys: 0.397 ± 0.018	1.481MetAsp: 1.481 ± 0.039	2.026MetGlu: 2.026 ± 0.046	0.966MetPhe: 0.966 ± 0.032	1.746MetGly: 1.746 ± 0.05	0.674MetHis: 0.674 ± 0.026	1.789MetIle: 1.789 ± 0.047	1.9MetLys: 1.9 ± 0.047	2.887MetLeu: 2.887 ± 0.059	0.82MetMet: 0.82 ± 0.034	1.2MetAsn: 1.2 ± 0.036	1.223MetPro: 1.223 ± 0.037	1.653MetGln: 1.653 ± 0.041	1.457MetArg: 1.457 ± 0.047	1.522MetSer: 1.522 ± 0.047	1.853MetThr: 1.853 ± 0.044	1.561MetVal: 1.561 ± 0.047	0.243MetTrp: 0.243 ± 0.016	0.903MetTyr: 0.903 ± 0.032	0.0MetXaa: 0.0 ± 0.0
Asn	3.77AsnAla: 3.77 ± 0.066	0.862AsnCys: 0.862 ± 0.033	2.227AsnAsp: 2.227 ± 0.059	2.166AsnGlu: 2.166 ± 0.056	1.556AsnPhe: 1.556 ± 0.042	3.779AsnGly: 3.779 ± 0.088	0.878AsnHis: 0.878 ± 0.036	2.696AsnIle: 2.696 ± 0.059	1.914AsnLys: 1.914 ± 0.05	3.492AsnLeu: 3.492 ± 0.063	1.098AsnMet: 1.098 ± 0.033	1.724AsnAsn: 1.724 ± 0.057	2.115AsnPro: 2.115 ± 0.051	1.507AsnGln: 1.507 ± 0.043	2.078AsnArg: 2.078 ± 0.052	2.443AsnSer: 2.443 ± 0.056	2.53AsnThr: 2.53 ± 0.065	2.666AsnVal: 2.666 ± 0.053	0.492AsnTrp: 0.492 ± 0.022	1.684AsnTyr: 1.684 ± 0.056	0.0AsnXaa: 0.0 ± 0.0
Pro	3.409ProAla: 3.409 ± 0.079	0.593ProCys: 0.593 ± 0.024	2.716ProAsp: 2.716 ± 0.063	3.823ProGlu: 3.823 ± 0.076	1.528ProPhe: 1.528 ± 0.041	1.402ProGly: 1.402 ± 0.046	0.775ProHis: 0.775 ± 0.028	1.942ProIle: 1.942 ± 0.051	2.149ProLys: 2.149 ± 0.045	2.955ProLeu: 2.955 ± 0.067	0.962ProMet: 0.962 ± 0.033	1.443ProAsn: 1.443 ± 0.044	1.217ProPro: 1.217 ± 0.042	1.536ProGln: 1.536 ± 0.04	1.234ProArg: 1.234 ± 0.039	1.883ProSer: 1.883 ± 0.043	1.86ProThr: 1.86 ± 0.047	3.089ProVal: 3.089 ± 0.058	0.343ProTrp: 0.343 ± 0.022	1.503ProTyr: 1.503 ± 0.047	0.0ProXaa: 0.0 ± 0.0
Gln	2.948GlnAla: 2.948 ± 0.062	0.593GlnCys: 0.593 ± 0.025	1.718GlnAsp: 1.718 ± 0.054	2.859GlnGlu: 2.859 ± 0.071	1.621GlnPhe: 1.621 ± 0.04	2.027GlnGly: 2.027 ± 0.041	0.799GlnHis: 0.799 ± 0.032	2.773GlnIle: 2.773 ± 0.061	3.155GlnLys: 3.155 ± 0.069	3.752GlnLeu: 3.752 ± 0.081	1.348GlnMet: 1.348 ± 0.039	2.099GlnAsn: 2.099 ± 0.044	1.465GlnPro: 1.465 ± 0.044	2.087GlnGln: 2.087 ± 0.074	1.755GlnArg: 1.755 ± 0.043	2.119GlnSer: 2.119 ± 0.048	2.424GlnThr: 2.424 ± 0.061	2.177GlnVal: 2.177 ± 0.051	0.448GlnTrp: 0.448 ± 0.02	1.717GlnTyr: 1.717 ± 0.05	0.0GlnXaa: 0.0 ± 0.0
Arg	2.9ArgAla: 2.9 ± 0.058	0.899ArgCys: 0.899 ± 0.035	2.174ArgAsp: 2.174 ± 0.055	3.169ArgGlu: 3.169 ± 0.072	2.107ArgPhe: 2.107 ± 0.054	2.457ArgGly: 2.457 ± 0.06	0.937ArgHis: 0.937 ± 0.035	3.308ArgIle: 3.308 ± 0.061	3.445ArgLys: 3.445 ± 0.079	4.11ArgLeu: 4.11 ± 0.078	1.437ArgMet: 1.437 ± 0.047	2.161ArgAsn: 2.161 ± 0.049	1.325ArgPro: 1.325 ± 0.044	2.333ArgGln: 2.333 ± 0.063	2.64ArgArg: 2.64 ± 0.068	2.551ArgSer: 2.551 ± 0.051	2.481ArgThr: 2.481 ± 0.052	2.585ArgVal: 2.585 ± 0.049	0.484ArgTrp: 0.484 ± 0.024	1.94ArgTyr: 1.94 ± 0.051	0.0ArgXaa: 0.0 ± 0.0
Ser	5.271SerAla: 5.271 ± 0.094	1.084SerCys: 1.084 ± 0.034	3.813SerAsp: 3.813 ± 0.083	3.979SerGlu: 3.979 ± 0.075	2.569SerPhe: 2.569 ± 0.06	5.39SerGly: 5.39 ± 0.09	1.18SerHis: 1.18 ± 0.039	3.826SerIle: 3.826 ± 0.089	3.165SerLys: 3.165 ± 0.064	5.13SerLeu: 5.13 ± 0.077	1.839SerMet: 1.839 ± 0.047	2.38SerAsn: 2.38 ± 0.049	2.002SerPro: 2.002 ± 0.044	1.867SerGln: 1.867 ± 0.045	2.694SerArg: 2.694 ± 0.059	3.945SerSer: 3.945 ± 0.091	3.492SerThr: 3.492 ± 0.075	4.741SerVal: 4.741 ± 0.079	0.631SerTrp: 0.631 ± 0.022	2.547SerTyr: 2.547 ± 0.053	0.0SerXaa: 0.0 ± 0.0
Thr	6.422ThrAla: 6.422 ± 0.121	0.898ThrCys: 0.898 ± 0.028	3.851ThrAsp: 3.851 ± 0.096	4.516ThrGlu: 4.516 ± 0.081	2.377ThrPhe: 2.377 ± 0.058	4.763ThrGly: 4.763 ± 0.075	0.996ThrHis: 0.996 ± 0.037	4.059ThrIle: 4.059 ± 0.077	3.16ThrLys: 3.16 ± 0.057	5.364ThrLeu: 5.364 ± 0.094	1.571ThrMet: 1.571 ± 0.042	2.155ThrAsn: 2.155 ± 0.064	2.653ThrPro: 2.653 ± 0.055	1.861ThrGln: 1.861 ± 0.051	2.002ThrArg: 2.002 ± 0.049	3.545ThrSer: 3.545 ± 0.092	5.187ThrThr: 5.187 ± 0.35	5.058ThrVal: 5.058 ± 0.091	0.613ThrTrp: 0.613 ± 0.029	2.366ThrTyr: 2.366 ± 0.074	0.0ThrXaa: 0.0 ± 0.0
Val	4.685ValAla: 4.685 ± 0.086	1.345ValCys: 1.345 ± 0.035	3.55ValAsp: 3.55 ± 0.068	3.828ValGlu: 3.828 ± 0.071	2.863ValPhe: 2.863 ± 0.058	3.936ValGly: 3.936 ± 0.075	1.274ValHis: 1.274 ± 0.04	4.096ValIle: 4.096 ± 0.066	3.601ValLys: 3.601 ± 0.067	6.843ValLeu: 6.843 ± 0.112	1.857ValMet: 1.857 ± 0.047	2.621ValAsn: 2.621 ± 0.055	2.838ValPro: 2.838 ± 0.053	2.908ValGln: 2.908 ± 0.063	3.197ValArg: 3.197 ± 0.063	4.737ValSer: 4.737 ± 0.073	4.762ValThr: 4.762 ± 0.086	4.47ValVal: 4.47 ± 0.121	0.739ValTrp: 0.739 ± 0.03	2.56ValTyr: 2.56 ± 0.057	0.0ValXaa: 0.0 ± 0.0
Trp	0.623TrpAla: 0.623 ± 0.026	0.194TrpCys: 0.194 ± 0.014	0.624TrpAsp: 0.624 ± 0.03	0.705TrpGlu: 0.705 ± 0.026	0.48TrpPhe: 0.48 ± 0.025	0.673TrpGly: 0.673 ± 0.033	0.248TrpHis: 0.248 ± 0.018	0.723TrpIle: 0.723 ± 0.028	0.753TrpLys: 0.753 ± 0.028	1.056TrpLeu: 1.056 ± 0.034	0.25TrpMet: 0.25 ± 0.017	0.737TrpAsn: 0.737 ± 0.032	0.111TrpPro: 0.111 ± 0.012	0.487TrpGln: 0.487 ± 0.023	0.423TrpArg: 0.423 ± 0.022	0.522TrpSer: 0.522 ± 0.024	0.545TrpThr: 0.545 ± 0.028	0.578TrpVal: 0.578 ± 0.028	0.126TrpTrp: 0.126 ± 0.012	0.491TrpTyr: 0.491 ± 0.024	0.0TrpXaa: 0.0 ± 0.0
Tyr	3.421TyrAla: 3.421 ± 0.066	0.824TyrCys: 0.824 ± 0.033	2.762TyrAsp: 2.762 ± 0.084	2.305TyrGlu: 2.305 ± 0.063	1.736TyrPhe: 1.736 ± 0.048	2.945TyrGly: 2.945 ± 0.067	0.965TyrHis: 0.965 ± 0.032	2.248TyrIle: 2.248 ± 0.052	1.535TyrLys: 1.535 ± 0.049	3.668TyrLeu: 3.668 ± 0.08	0.872TyrMet: 0.872 ± 0.032	1.812TyrAsn: 1.812 ± 0.05	1.46TyrPro: 1.46 ± 0.04	1.816TyrGln: 1.816 ± 0.049	2.141TyrArg: 2.141 ± 0.053	2.359TyrSer: 2.359 ± 0.057	2.644TyrThr: 2.644 ± 0.064	2.419TyrVal: 2.419 ± 0.058	0.402TyrTrp: 0.402 ± 0.024	1.959TyrTyr: 1.959 ± 0.066	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.0XaaAla: 0.0 ± 0.0	0.0XaaCys: 0.0 ± 0.0	0.0XaaAsp: 0.0 ± 0.0	0.0XaaGlu: 0.0 ± 0.0	0.0XaaPhe: 0.0 ± 0.0	0.0XaaGly: 0.0 ± 0.0	0.0XaaHis: 0.0 ± 0.0	0.0XaaIle: 0.0 ± 0.0	0.0XaaLys: 0.0 ± 0.0	0.0XaaLeu: 0.0 ± 0.0	0.0XaaMet: 0.0 ± 0.0	0.0XaaAsn: 0.0 ± 0.0	0.0XaaPro: 0.0 ± 0.0	0.0XaaGln: 0.0 ± 0.0	0.0XaaArg: 0.0 ± 0.0	0.0XaaSer: 0.0 ± 0.0	0.0XaaThr: 0.0 ± 0.0	0.0XaaVal: 0.0 ± 0.0	0.0XaaTrp: 0.0 ± 0.0	0.0XaaTyr: 0.0 ± 0.0	0.0XaaXaa: 0.0 ± 0.0

Statistics based on 3475 proteins (928887 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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