Vibrio phage ICP2_2013_A_Haiti
Average proteome isoelectric point is 6.01
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 57 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A076G5G2|A0A076G5G2_9CAUD Uncharacterized protein OS=Vibrio phage ICP2_2013_A_Haiti OX=1529058 GN=ICP22013AHaiti_34 PE=4 SV=1
MM1 pKa = 7.7 SINLSSISIGTKK13 pKa = 9.7 VRR15 pKa = 11.84 GLSRR19 pKa = 11.84 PDD21 pKa = 4.21 DD22 pKa = 4.44 LDD24 pKa = 4.94 DD25 pKa = 5.46 GYY27 pKa = 11.4 LFSEE31 pKa = 4.36 DD32 pKa = 4.15 CILEE36 pKa = 4.18 RR37 pKa = 11.84 EE38 pKa = 4.25 EE39 pKa = 3.86 EE40 pKa = 4.33 SAVIIAIFGVADD52 pKa = 4.23 LSDD55 pKa = 4.35 PYY57 pKa = 11.19 AAVPFKK63 pKa = 10.57 ILSGTVDD70 pKa = 3.81 DD71 pKa = 5.11 LAGSVYY77 pKa = 10.11 TVGDD81 pKa = 4.01 TVHH84 pKa = 7.04 LDD86 pKa = 3.32 ANDD89 pKa = 3.17 ILEE92 pKa = 4.29 IVEE95 pKa = 4.06
Molecular weight: 10.18 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.114
IPC2_protein 3.757
IPC_protein 3.719
Toseland 3.503
ProMoST 3.859
Dawson 3.719
Bjellqvist 3.935
Wikipedia 3.668
Rodwell 3.554
Grimsley 3.414
Solomon 3.694
Lehninger 3.656
Nozaki 3.846
DTASelect 4.075
Thurlkill 3.579
EMBOSS 3.681
Sillero 3.846
Patrickios 3.249
IPC_peptide 3.694
IPC2_peptide 3.808
IPC2.peptide.svr19 3.786
Protein with the highest isoelectric point:
>tr|A0A076G594|A0A076G594_9CAUD Nmad5 domain-containing protein OS=Vibrio phage ICP2_2013_A_Haiti OX=1529058 GN=ICP22013AHaiti_36 PE=4 SV=1
MM1 pKa = 7.31 KK2 pKa = 10.23 QRR4 pKa = 11.84 NPNRR8 pKa = 11.84 AHH10 pKa = 5.66 SPEE13 pKa = 3.78 RR14 pKa = 11.84 LMLRR18 pKa = 11.84 KK19 pKa = 8.35 QTADD23 pKa = 2.91 VGLIKK28 pKa = 10.53 VVNLISRR35 pKa = 11.84 ATHH38 pKa = 5.52 GAVFGGRR45 pKa = 11.84 LGFICAAIKK54 pKa = 10.24 QDD56 pKa = 3.25 GANIRR61 pKa = 11.84 SLSTWEE67 pKa = 4.86 AYY69 pKa = 9.18 GQVYY73 pKa = 10.82 DD74 pKa = 4.53 SVTTMAIIHH83 pKa = 6.32
Molecular weight: 9.15 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.307
IPC2_protein 9.575
IPC_protein 10.101
Toseland 10.467
ProMoST 10.116
Dawson 10.599
Bjellqvist 10.277
Wikipedia 10.774
Rodwell 10.906
Grimsley 10.657
Solomon 10.672
Lehninger 10.657
Nozaki 10.467
DTASelect 10.262
Thurlkill 10.482
EMBOSS 10.862
Sillero 10.526
Patrickios 10.716
IPC_peptide 10.672
IPC2_peptide 9.18
IPC2.peptide.svr19 8.599
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
57
0
57
13737
58
1416
241.0
26.84
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
7.964 ± 0.47
0.903 ± 0.157
6.435 ± 0.295
6.857 ± 0.331
3.822 ± 0.145
7.76 ± 0.625
1.434 ± 0.173
5.54 ± 0.194
6.231 ± 0.341
7.36 ± 0.299
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.715 ± 0.199
4.826 ± 0.221
3.938 ± 0.35
4.171 ± 0.366
4.805 ± 0.262
6.391 ± 0.268
6.341 ± 0.281
7.192 ± 0.337
1.478 ± 0.121
3.836 ± 0.175
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here