SAR11 cluster bacterium PRT-SC02

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Alphaproteobacteria; Pelagibacterales; Pelagibacteraceae; unclassified Pelagibacteraceae

Average proteome isoelectric point is 8.24

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 632 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0P9CG28|A0A0P9CG28_9PROT Uncharacterized protein OS=SAR11 cluster bacterium PRT-SC02 OX=1527298 GN=JI56_03950 PE=4 SV=1
MM1 pKa = 7.5KK2 pKa = 10.42GKK4 pKa = 10.48DD5 pKa = 3.22MTKK8 pKa = 8.93LTKK11 pKa = 10.32IMLAMIASLSLLSTSYY27 pKa = 10.88AGEE30 pKa = 4.03VTVTGNAEE38 pKa = 3.77ATYY41 pKa = 9.98SISGAKK47 pKa = 9.07TGTGVDD53 pKa = 3.63DD54 pKa = 4.01TDD56 pKa = 3.78TVGGKK61 pKa = 10.5GIGVANEE68 pKa = 3.63IDD70 pKa = 3.84FTATGEE76 pKa = 4.17TDD78 pKa = 4.09NGYY81 pKa = 6.38TWKK84 pKa = 10.68YY85 pKa = 6.68QTQFDD90 pKa = 4.71DD91 pKa = 5.95GSATDD96 pKa = 4.21DD97 pKa = 3.46GRR99 pKa = 11.84LEE101 pKa = 3.9ITSPFGTVGFYY112 pKa = 10.82VSEE115 pKa = 4.5GSLGSHH121 pKa = 6.38YY122 pKa = 10.81GWDD125 pKa = 3.38VSAYY129 pKa = 10.34GAGSDD134 pKa = 4.76QGITTDD140 pKa = 3.8AEE142 pKa = 4.31INGIEE147 pKa = 3.95ASDD150 pKa = 3.81YY151 pKa = 11.83NNIQYY156 pKa = 7.51HH157 pKa = 5.48TPADD161 pKa = 4.02LLPFGIQAKK170 pKa = 9.89VAFAPNGATGQTKK183 pKa = 9.8AASVRR188 pKa = 11.84AQGANTADD196 pKa = 3.27IAEE199 pKa = 4.38ATSYY203 pKa = 10.91QITATPIDD211 pKa = 4.29GLSVGASYY219 pKa = 11.67AEE221 pKa = 4.03ATHH224 pKa = 6.3NGGVTATKK232 pKa = 9.58YY233 pKa = 9.95QPEE236 pKa = 4.2NGSLYY241 pKa = 10.03VKK243 pKa = 10.2YY244 pKa = 10.42AIGPATIGYY253 pKa = 8.09GKK255 pKa = 9.0TYY257 pKa = 9.39IQPRR261 pKa = 11.84VAARR265 pKa = 11.84TADD268 pKa = 3.27TDD270 pKa = 3.78TTNEE274 pKa = 3.36ARR276 pKa = 11.84KK277 pKa = 10.08YY278 pKa = 9.94EE279 pKa = 4.06NTSYY283 pKa = 11.7GLGVSVNDD291 pKa = 3.51QLSVSFTTEE300 pKa = 3.58EE301 pKa = 4.25FEE303 pKa = 4.19LTEE306 pKa = 3.83VDD308 pKa = 3.68VAVATDD314 pKa = 4.1AEE316 pKa = 4.89TSATPVINVDD326 pKa = 4.38TIQAAYY332 pKa = 9.89NVGGMTVAIGHH343 pKa = 6.75AEE345 pKa = 3.89ADD347 pKa = 3.98EE348 pKa = 4.07ISYY351 pKa = 11.2NGDD354 pKa = 2.99NATANDD360 pKa = 3.85EE361 pKa = 4.33HH362 pKa = 7.3DD363 pKa = 3.68TTQTFISVKK372 pKa = 9.52MAFF375 pKa = 3.67

Molecular weight: 39.11 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0P9A876|A0A0P9A876_9PROT Phytoene synthase OS=SAR11 cluster bacterium PRT-SC02 OX=1527298 GN=JI56_00990 PE=4 SV=1
SS1 pKa = 6.13TGGRR5 pKa = 11.84NNYY8 pKa = 8.87GRR10 pKa = 11.84ITSRR14 pKa = 11.84HH15 pKa = 5.43RR16 pKa = 11.84GGGAKK21 pKa = 9.36HH22 pKa = 6.82KK23 pKa = 10.65YY24 pKa = 9.96RR25 pKa = 11.84IIDD28 pKa = 3.83FYY30 pKa = 10.74RR31 pKa = 11.84NKK33 pKa = 9.76MNTKK37 pKa = 8.45ATVEE41 pKa = 4.13RR42 pKa = 11.84IEE44 pKa = 4.07YY45 pKa = 10.21DD46 pKa = 3.48PNRR49 pKa = 11.84TAHH52 pKa = 6.71IALINYY58 pKa = 7.4EE59 pKa = 4.31NNIKK63 pKa = 10.66NYY65 pKa = 9.28ILCPQGLKK73 pKa = 10.51KK74 pKa = 10.72GDD76 pKa = 3.83IIEE79 pKa = 4.51SGSNIEE85 pKa = 4.49IKK87 pKa = 10.36IGNNLKK93 pKa = 10.61LRR95 pKa = 11.84DD96 pKa = 3.96IPVGTSVHH104 pKa = 5.73NVEE107 pKa = 4.57LTPGGGGKK115 pKa = 9.91LARR118 pKa = 11.84SAGASVVLSGYY129 pKa = 10.69DD130 pKa = 3.12GDD132 pKa = 4.05YY133 pKa = 11.27AIIKK137 pKa = 9.59LSSGEE142 pKa = 4.05TRR144 pKa = 11.84KK145 pKa = 9.66IRR147 pKa = 11.84SDD149 pKa = 3.45CSATIGVVSNPDD161 pKa = 3.14QKK163 pKa = 11.12NIKK166 pKa = 9.04IGKK169 pKa = 8.76AGRR172 pKa = 11.84NRR174 pKa = 11.84WRR176 pKa = 11.84GIRR179 pKa = 11.84PQSRR183 pKa = 11.84GVAMNPVDD191 pKa = 3.85HH192 pKa = 6.82PHH194 pKa = 6.77GGGEE198 pKa = 4.38GKK200 pKa = 9.0TSGGRR205 pKa = 11.84SPVSPWGQSAKK216 pKa = 10.4GLKK219 pKa = 7.63TRR221 pKa = 11.84KK222 pKa = 9.09PKK224 pKa = 10.68RR225 pKa = 11.84SDD227 pKa = 2.68KK228 pKa = 10.96FIISRR233 pKa = 11.84RR234 pKa = 11.84KK235 pKa = 8.88KK236 pKa = 10.22RR237 pKa = 11.84KK238 pKa = 8.99

Molecular weight: 26.01 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

632

0

632

174364

36

1499

275.9

31.35

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.154 ± 0.102

0.979 ± 0.031

4.928 ± 0.073

5.74 ± 0.096

5.516 ± 0.097

5.959 ± 0.09

1.49 ± 0.041

9.953 ± 0.11

11.799 ± 0.154

9.472 ± 0.101

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.018 ± 0.04

6.807 ± 0.107

3.044 ± 0.046

2.552 ± 0.049

3.367 ± 0.063

6.979 ± 0.076

4.486 ± 0.054

5.454 ± 0.069

0.884 ± 0.035

3.419 ± 0.06
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski