Legionella worsleiensis

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Gammaproteobacteria; Legionellales; Legionellaceae; Legionella

Average proteome isoelectric point is 6.63

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2614 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0W1A3H8|A0A0W1A3H8_9GAMM SidA protein subsrate of the Dot/Icm transport system OS=Legionella worsleiensis OX=45076 GN=sidA PE=4 SV=1
MM1 pKa = 6.95VANGSVVLNADD12 pKa = 3.52GTLSFTPAADD22 pKa = 3.91FNGNASFTYY31 pKa = 9.12TVTSAGLTEE40 pKa = 4.13TATVNVTVNPVNEE53 pKa = 4.1APINSTPGAQTLSEE67 pKa = 4.14DD68 pKa = 3.28ATRR71 pKa = 11.84VFSSANGNALSVTDD85 pKa = 3.72IDD87 pKa = 4.17SAVVTTTISVLHH99 pKa = 6.42GSLTALATAGVTISNNGTGSVTLSGAPAAINTALNGLIYY138 pKa = 10.77SPVADD143 pKa = 3.58YY144 pKa = 11.0HH145 pKa = 7.57GSDD148 pKa = 3.96TLTMSTTDD156 pKa = 3.71GSLLDD161 pKa = 4.09SDD163 pKa = 4.54TVGITINPVVDD174 pKa = 3.58IANDD178 pKa = 4.09TIVTNEE184 pKa = 3.82DD185 pKa = 3.22TAVIVNVNANDD196 pKa = 3.59SFEE199 pKa = 4.44NAGHH203 pKa = 7.32TITAINGTTIAVGGTVAVADD223 pKa = 4.25GSVLLNANGTLTFTPSANYY242 pKa = 10.5NGTTNFTYY250 pKa = 10.0TVTSGGVTEE259 pKa = 4.24TASVNVTVNAVNDD272 pKa = 3.85APQGQNNLLSVNEE285 pKa = 4.21DD286 pKa = 3.59TPIVLSAANFNFTDD300 pKa = 4.08TDD302 pKa = 3.61DD303 pKa = 3.93TPANTFAGVRR313 pKa = 11.84ITTLPANGTLTLNGVAVVAGQTISIANINAGLLVFTPTADD353 pKa = 3.54YY354 pKa = 11.06SGQTNFTFQVEE365 pKa = 4.73DD366 pKa = 3.29NGGTANGGVNLDD378 pKa = 3.46PTPNVMTVDD387 pKa = 3.72VASVADD393 pKa = 3.9TPVVFAHH400 pKa = 6.97LSLPTLVAGTAVIAANFSNNSNEE423 pKa = 4.09NNLSNWSRR431 pKa = 11.84VALFSNTFAEE441 pKa = 4.38IQGGSGKK448 pKa = 8.94TNTEE452 pKa = 3.91ANLFSQIVWAGEE464 pKa = 3.93TGTNATEE471 pKa = 3.82VSNFNNRR478 pKa = 11.84WTKK481 pKa = 9.88PSGTIIYY488 pKa = 8.56NANEE492 pKa = 4.18VADD495 pKa = 4.39DD496 pKa = 3.89AQGILVLNSNQLTVAEE512 pKa = 4.33RR513 pKa = 11.84ALTNYY518 pKa = 9.3VLSVDD523 pKa = 4.06MFADD527 pKa = 3.89ADD529 pKa = 3.91SPRR532 pKa = 11.84ANGTGIVFGYY542 pKa = 10.51QNASNYY548 pKa = 10.17FLVRR552 pKa = 11.84WEE554 pKa = 4.36NPSIEE559 pKa = 4.07YY560 pKa = 10.77SPGGEE565 pKa = 4.09FFNVHH570 pKa = 6.38PGQYY574 pKa = 9.65QEE576 pKa = 4.07LTLVQVVGNVPIDD589 pKa = 3.59LARR592 pKa = 11.84TTFNADD598 pKa = 2.32DD599 pKa = 3.84WFNVVINVSNLGISVSASDD618 pKa = 3.96MSNPSITANFDD629 pKa = 3.67YY630 pKa = 10.52IYY632 pKa = 10.79GSVSGAAAAAPALRR646 pKa = 11.84TIGLYY651 pKa = 9.93TYY653 pKa = 10.71DD654 pKa = 4.11NDD656 pKa = 5.9SAVQWDD662 pKa = 4.67NINVNQPSSYY672 pKa = 10.72QYY674 pKa = 9.96TLQTEE679 pKa = 5.19AYY681 pKa = 9.64LKK683 pKa = 11.08DD684 pKa = 3.56NDD686 pKa = 4.05GSEE689 pKa = 4.26TLGNITLAGIPAGVTLLDD707 pKa = 3.62VVTNTSIPVNAGSATIIPGNDD728 pKa = 2.71VRR730 pKa = 11.84LVSSTALTTAQINAITATVSSVEE753 pKa = 4.13SSNGSTASQTTSVQLEE769 pKa = 4.47LNGGYY774 pKa = 10.52APEE777 pKa = 4.54TFTGTNASEE786 pKa = 3.99WLEE789 pKa = 4.06GDD791 pKa = 3.59SSNDD795 pKa = 2.9ILNAGGGNDD804 pKa = 4.08TIIGGLGNDD813 pKa = 4.51TITTGSGSDD822 pKa = 3.56QIVLLKK828 pKa = 10.66GQGGATAGAAPTDD841 pKa = 3.98TITDD845 pKa = 3.75FTVNVDD851 pKa = 3.8TIVIKK856 pKa = 9.29GTNIIGVAVSTPVSNTYY873 pKa = 9.87TITVNYY879 pKa = 9.98SGGAPTEE886 pKa = 4.19YY887 pKa = 10.66FKK889 pKa = 10.96VTLSNGAVLNDD900 pKa = 3.82SGDD903 pKa = 3.36THH905 pKa = 6.09LTGVVVSGGTATIDD919 pKa = 3.44GTIVGAILYY928 pKa = 10.51LDD930 pKa = 3.64INSNNQEE937 pKa = 4.14DD938 pKa = 3.64EE939 pKa = 4.28GEE941 pKa = 4.1RR942 pKa = 11.84LGITDD947 pKa = 3.25QYY949 pKa = 11.69GHH951 pKa = 5.83VEE953 pKa = 4.05WVLDD957 pKa = 4.07LAKK960 pKa = 10.59LDD962 pKa = 3.95VNGDD966 pKa = 3.38GQYY969 pKa = 11.55VLGEE973 pKa = 3.89ARR975 pKa = 11.84AVQTGGFDD983 pKa = 3.3IDD985 pKa = 3.62TGLSYY990 pKa = 10.52EE991 pKa = 4.32INLFGPVGSAIVSPLTSLLQAQLEE1015 pKa = 4.6SGFDD1019 pKa = 3.63YY1020 pKa = 9.89QTANANIVARR1030 pKa = 11.84LGLPEE1035 pKa = 4.12GTEE1038 pKa = 4.5LISLNPIMGSHH1049 pKa = 7.52DD1050 pKa = 3.66VLAQNAAVMTAAVQFAEE1067 pKa = 4.37IAAIRR1072 pKa = 11.84YY1073 pKa = 7.3STDD1076 pKa = 2.98EE1077 pKa = 3.98GHH1079 pKa = 6.83VSFSVFEE1086 pKa = 5.04AISKK1090 pKa = 10.58ALLEE1094 pKa = 4.35LPEE1097 pKa = 4.26GVIADD1102 pKa = 4.61FSDD1105 pKa = 3.54KK1106 pKa = 11.39AFLQNIASHH1115 pKa = 6.97LDD1117 pKa = 3.52LGDD1120 pKa = 3.45MASPEE1125 pKa = 4.48VIAFMAASQQALQSSMDD1142 pKa = 3.74TLVPGQNALAAISEE1156 pKa = 4.54VQHH1159 pKa = 5.88LTQGSYY1165 pKa = 10.62AQIIEE1170 pKa = 4.73HH1171 pKa = 5.39YY1172 pKa = 8.84TNGYY1176 pKa = 9.1LPAYY1180 pKa = 8.92LLSDD1184 pKa = 3.52ISVIILAFSSSIISYY1199 pKa = 10.98DD1200 pKa = 3.43EE1201 pKa = 4.14LKK1203 pKa = 11.11GFNDD1207 pKa = 3.81QFNTLVNQSAIMLSDD1222 pKa = 3.56GSEE1225 pKa = 4.05SSNKK1229 pKa = 9.93DD1230 pKa = 3.48DD1231 pKa = 5.06FVHH1234 pKa = 6.6EE1235 pKa = 4.44LDD1237 pKa = 3.72SVVNNFVLAHH1247 pKa = 6.18GLDD1250 pKa = 3.7VSHH1253 pKa = 6.66ATDD1256 pKa = 4.1GQQSLDD1262 pKa = 3.91DD1263 pKa = 3.48QHH1265 pKa = 6.67MNALINQSVEE1275 pKa = 4.06DD1276 pKa = 4.4GNVLAHH1282 pKa = 6.08EE1283 pKa = 4.64VNNHH1287 pKa = 5.98EE1288 pKa = 5.08IITDD1292 pKa = 4.31LSSSIGGMEE1301 pKa = 3.9LHH1303 pKa = 6.68GSSGNDD1309 pKa = 3.45FIFGGEE1315 pKa = 4.25GNDD1318 pKa = 4.56LILGGEE1324 pKa = 4.28GDD1326 pKa = 3.74DD1327 pKa = 4.61RR1328 pKa = 11.84LYY1330 pKa = 11.22GGQGDD1335 pKa = 4.17DD1336 pKa = 4.85SILGGLGDD1344 pKa = 4.32DD1345 pKa = 4.58VIHH1348 pKa = 7.0GGMGNDD1354 pKa = 3.74LMTGGGGHH1362 pKa = 7.59DD1363 pKa = 2.59IFVFEE1368 pKa = 4.64KK1369 pKa = 10.79ADD1371 pKa = 3.41IGEE1374 pKa = 4.32EE1375 pKa = 3.93PAIDD1379 pKa = 4.12TITDD1383 pKa = 4.41FKK1385 pKa = 11.37LGIAGDD1391 pKa = 3.82NSGDD1395 pKa = 3.91KK1396 pKa = 9.88STLDD1400 pKa = 3.28LTDD1403 pKa = 4.14LFKK1406 pKa = 11.2EE1407 pKa = 4.41STLSSDD1413 pKa = 4.49SLDD1416 pKa = 3.38TLLQISTIHH1425 pKa = 6.46NEE1427 pKa = 4.12STNQTDD1433 pKa = 3.86TVIKK1437 pKa = 9.85TDD1439 pKa = 3.51PTGNAQFNNSPEE1451 pKa = 4.2TIILSGVDD1459 pKa = 3.17VASAYY1464 pKa = 7.66GTTNSVEE1471 pKa = 4.37LVQHH1475 pKa = 5.92MLAANVLVMGHH1486 pKa = 6.38

Molecular weight: 154.03 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0W1AEJ3|A0A0W1AEJ3_9GAMM Substrate of the Dot/Icm secretion system OS=Legionella worsleiensis OX=45076 GN=pieE_1 PE=4 SV=1
MM1 pKa = 7.82SINTRR6 pKa = 11.84LKK8 pKa = 10.34SIAVLLVSAMLTGCVVAVVAGAAAGMVYY36 pKa = 10.48DD37 pKa = 4.3RR38 pKa = 11.84RR39 pKa = 11.84SVTTIEE45 pKa = 3.47ADD47 pKa = 2.92ARR49 pKa = 11.84LFHH52 pKa = 6.67VIHH55 pKa = 6.77KK56 pKa = 9.53EE57 pKa = 3.83IVTDD61 pKa = 3.79PQFRR65 pKa = 11.84NSRR68 pKa = 11.84ILVTSFNRR76 pKa = 11.84VVLLVGQTPTASLRR90 pKa = 11.84VVAEE94 pKa = 4.26RR95 pKa = 11.84VAQNAPGVRR104 pKa = 11.84RR105 pKa = 11.84VYY107 pKa = 11.17DD108 pKa = 4.65EE109 pKa = 4.05ITVDD113 pKa = 3.62NPIPLTQRR121 pKa = 11.84SQDD124 pKa = 3.53SLITGQVRR132 pKa = 11.84SNMLTKK138 pKa = 10.47KK139 pKa = 9.98GLEE142 pKa = 4.04SGSIRR147 pKa = 11.84IVTEE151 pKa = 3.49NGVVYY156 pKa = 10.59LMGIVTPEE164 pKa = 3.86QASLAVDD171 pKa = 3.58VARR174 pKa = 11.84RR175 pKa = 11.84INGVRR180 pKa = 11.84KK181 pKa = 9.14VVKK184 pKa = 9.21IFQYY188 pKa = 10.51IRR190 pKa = 3.21

Molecular weight: 20.78 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2614

0

2614

893477

51

3762

341.8

38.29

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.835 ± 0.05

1.261 ± 0.018

4.99 ± 0.037

5.712 ± 0.047

4.373 ± 0.039

6.003 ± 0.053

2.713 ± 0.023

7.085 ± 0.036

5.805 ± 0.053

11.04 ± 0.063

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.413 ± 0.026

4.867 ± 0.039

4.115 ± 0.026

4.67 ± 0.04

4.25 ± 0.037

6.763 ± 0.039

5.439 ± 0.04

6.142 ± 0.047

1.095 ± 0.017

3.423 ± 0.033
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski