Bacteroidales bacterium KA00251

Taxonomy: cellular organisms; Bacteria; FCB group; Bacteroidetes/Chlorobi group; Bacteroidetes; Bacteroidia; Bacteroidales; unclassified Bacteroidales

Average proteome isoelectric point is 7.01

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 1795 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A133XTB7|A0A133XTB7_9BACT DNA (Cytosine-5-)-methyltransferase OS=Bacteroidales bacterium KA00251 OX=1497953 GN=HMPREF1869_01281 PE=3 SV=1
MM1 pKa = 7.54NDD3 pKa = 2.69ISQILLGNKK12 pKa = 9.55AIQSLFDD19 pKa = 4.0KK20 pKa = 11.34YY21 pKa = 11.49NLTDD25 pKa = 3.61EE26 pKa = 5.21FVATPEE32 pKa = 3.75YY33 pKa = 9.18DD34 pKa = 3.23TLYY37 pKa = 10.69TEE39 pKa = 4.26LTGTVVLLIEE49 pKa = 5.08ANQLPTIGDD58 pKa = 3.52VSALEE63 pKa = 3.98

Molecular weight: 6.96 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A133XV20|A0A133XV20_9BACT SNARE-like domain protein OS=Bacteroidales bacterium KA00251 OX=1497953 GN=HMPREF1869_01075 PE=4 SV=1
MM1 pKa = 7.19IQQEE5 pKa = 4.3SRR7 pKa = 11.84LNVADD12 pKa = 3.52NSGAKK17 pKa = 9.22EE18 pKa = 3.93VLCIRR23 pKa = 11.84VLGGTRR29 pKa = 11.84RR30 pKa = 11.84RR31 pKa = 11.84YY32 pKa = 10.35AGVGDD37 pKa = 4.04VIVVTVKK44 pKa = 10.81SVVPTSDD51 pKa = 2.61IKK53 pKa = 11.1KK54 pKa = 8.03GTVSRR59 pKa = 11.84ALIVRR64 pKa = 11.84TKK66 pKa = 10.75KK67 pKa = 10.23PIRR70 pKa = 11.84RR71 pKa = 11.84ADD73 pKa = 3.12GSYY76 pKa = 10.63IRR78 pKa = 11.84FGDD81 pKa = 3.8NACVLLTSSGEE92 pKa = 3.93LRR94 pKa = 11.84GTRR97 pKa = 11.84IFGPVARR104 pKa = 11.84EE105 pKa = 3.49LRR107 pKa = 11.84QANMKK112 pKa = 9.63VVSLAPEE119 pKa = 4.0VLL121 pKa = 3.56

Molecular weight: 13.12 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

1795

0

1795

553800

37

2515

308.5

34.72

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.082 ± 0.054

1.116 ± 0.022

4.914 ± 0.038

7.003 ± 0.062

4.532 ± 0.036

6.501 ± 0.048

2.026 ± 0.027

6.631 ± 0.049

6.852 ± 0.044

10.399 ± 0.068

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.287 ± 0.029

4.23 ± 0.04

4.153 ± 0.036

3.589 ± 0.03

5.01 ± 0.039

6.986 ± 0.047

5.551 ± 0.044

6.235 ± 0.045

0.938 ± 0.02

3.966 ± 0.039
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski