Glycomyces sambucus

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Actinomycetia; Glycomycetales; Glycomycetaceae; Glycomyces

Average proteome isoelectric point is 5.95

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 5037 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1G9CNK5|A0A1G9CNK5_9ACTN Uncharacterized protein OS=Glycomyces sambucus OX=380244 GN=SAMN05216298_0435 PE=4 SV=1
MM1 pKa = 7.09HH2 pKa = 7.45RR3 pKa = 11.84YY4 pKa = 8.44VRR6 pKa = 11.84RR7 pKa = 11.84GLVTAVMSGGLILLGSSMASADD29 pKa = 3.83EE30 pKa = 4.38GSGDD34 pKa = 3.98LLGLGGVVDD43 pKa = 4.61SVTSGLTGDD52 pKa = 4.47DD53 pKa = 3.66QGDD56 pKa = 3.93TASGDD61 pKa = 3.29ASADD65 pKa = 3.77GNSDD69 pKa = 3.09AAANGGNGGDD79 pKa = 3.83SGNAGDD85 pKa = 4.09ATSDD89 pKa = 3.35NTATSGNSGDD99 pKa = 4.31SGNSGDD105 pKa = 3.77TGGNSALNVCVLAEE119 pKa = 4.08CHH121 pKa = 6.31ASADD125 pKa = 4.07SGDD128 pKa = 3.95SGDD131 pKa = 3.92TGSTGDD137 pKa = 3.85TGDD140 pKa = 4.02AANSSHH146 pKa = 6.26TSGGNSGDD154 pKa = 3.46AGEE157 pKa = 5.22GGNADD162 pKa = 3.88AGAEE166 pKa = 4.15SGNTADD172 pKa = 5.2SGEE175 pKa = 4.3VWSEE179 pKa = 4.03TGWFTDD185 pKa = 3.36SSGDD189 pKa = 3.66TASGDD194 pKa = 3.38ASADD198 pKa = 3.57GDD200 pKa = 3.86SDD202 pKa = 3.93AAATGGNGGDD212 pKa = 3.88SGDD215 pKa = 4.35AGDD218 pKa = 4.03ATSDD222 pKa = 3.37NTATSGNSGDD232 pKa = 4.29SGDD235 pKa = 4.04SGSTGGNSALNLCVLAEE252 pKa = 4.17CHH254 pKa = 6.56ASADD258 pKa = 4.05SGNSGDD264 pKa = 3.94TGSTGDD270 pKa = 4.93TGNAWNTSHH279 pKa = 7.13TNGGHH284 pKa = 6.2SGDD287 pKa = 4.51AGDD290 pKa = 5.19GGNADD295 pKa = 4.37AEE297 pKa = 4.34AGSWNSAHH305 pKa = 7.06IGDD308 pKa = 4.22AVSDD312 pKa = 3.9NGNDD316 pKa = 2.69WWHH319 pKa = 5.53FTGQNAHH326 pKa = 5.84GWHH329 pKa = 6.91HH330 pKa = 6.34GTTGDD335 pKa = 3.87TASGDD340 pKa = 3.42ATAGGDD346 pKa = 3.4SDD348 pKa = 4.76AAATGGNGGTSGDD361 pKa = 3.92AGDD364 pKa = 3.72ATSANTATTGDD375 pKa = 4.05SGDD378 pKa = 4.1SGNSGDD384 pKa = 3.91TGGNTALGLGEE395 pKa = 4.45SDD397 pKa = 3.6QNRR400 pKa = 11.84HH401 pKa = 5.64GWHH404 pKa = 6.48HH405 pKa = 6.54WCGCWKK411 pKa = 10.42HH412 pKa = 6.91DD413 pKa = 4.12DD414 pKa = 3.84ASSTEE419 pKa = 4.16VNSGDD424 pKa = 4.08SGNTGWTGTTGDD436 pKa = 3.64AWNTSHH442 pKa = 6.87TMGGHH447 pKa = 6.23SGDD450 pKa = 3.94AGNGGDD456 pKa = 3.6AWAGAWSANTAHH468 pKa = 6.72TGWAHH473 pKa = 6.32AVV475 pKa = 3.26

Molecular weight: 44.85 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1G9GPQ1|A0A1G9GPQ1_9ACTN Glycosyltransferase involved in cell wall bisynthesis OS=Glycomyces sambucus OX=380244 GN=SAMN05216298_2340 PE=4 SV=1
MM1 pKa = 7.4GSVIKK6 pKa = 10.42KK7 pKa = 8.47RR8 pKa = 11.84RR9 pKa = 11.84KK10 pKa = 9.22RR11 pKa = 11.84MAKK14 pKa = 9.41KK15 pKa = 9.87KK16 pKa = 9.7HH17 pKa = 5.84RR18 pKa = 11.84KK19 pKa = 8.7LLRR22 pKa = 11.84KK23 pKa = 7.95TRR25 pKa = 11.84VQRR28 pKa = 11.84RR29 pKa = 11.84KK30 pKa = 10.07LGKK33 pKa = 9.87

Molecular weight: 4.1 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

5037

0

5037

1656086

27

3202

328.8

35.17

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

14.397 ± 0.055

0.697 ± 0.009

6.412 ± 0.036

6.069 ± 0.035

3.084 ± 0.02

9.27 ± 0.032

2.077 ± 0.016

3.645 ± 0.024

1.982 ± 0.023

10.134 ± 0.039

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.841 ± 0.015

1.967 ± 0.021

5.674 ± 0.032

2.592 ± 0.02

7.314 ± 0.045

4.825 ± 0.025

5.855 ± 0.027

8.304 ± 0.032

1.673 ± 0.015

2.189 ± 0.016
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski