Propionispira arboris

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Negativicutes; Selenomonadales; Selenomonadaceae; Propionispira

Average proteome isoelectric point is 6.43

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3963 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1H7A893|A0A1H7A893_9FIRM Uncharacterized protein OS=Propionispira arboris OX=84035 GN=SAMN05660742_111112 PE=4 SV=1
MM1 pKa = 7.67ILTEE5 pKa = 4.08MNAANALNYY14 pKa = 9.5IDD16 pKa = 4.8PADD19 pKa = 3.81MPEE22 pKa = 4.11KK23 pKa = 10.61VVTIYY28 pKa = 11.03LPAIDD33 pKa = 5.4NYY35 pKa = 9.58LTNATGKK42 pKa = 10.15DD43 pKa = 3.07WGILTDD49 pKa = 4.17TYY51 pKa = 11.08TAIDD55 pKa = 4.06PLAVMIASVILVTWFEE71 pKa = 5.06DD72 pKa = 3.97PSQMGKK78 pKa = 10.12VSNNGLLAMIGQLHH92 pKa = 6.23SKK94 pKa = 10.82YY95 pKa = 8.9MVEE98 pKa = 4.52SATLL102 pKa = 3.3

Molecular weight: 11.15 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1H7B828|A0A1H7B828_9FIRM Amino acid/amide ABC transporter substrate-binding protein HAAT family (TC 3.A.1.4.-) OS=Propionispira arboris OX=84035 GN=SAMN05660742_11588 PE=3 SV=1
MM1 pKa = 7.35KK2 pKa = 9.4RR3 pKa = 11.84TYY5 pKa = 10.31QPNTLWRR12 pKa = 11.84KK13 pKa = 6.72RR14 pKa = 11.84THH16 pKa = 6.25GFRR19 pKa = 11.84EE20 pKa = 3.85RR21 pKa = 11.84MKK23 pKa = 9.06TQGGRR28 pKa = 11.84LVLKK32 pKa = 10.11RR33 pKa = 11.84RR34 pKa = 11.84RR35 pKa = 11.84VRR37 pKa = 11.84GRR39 pKa = 11.84KK40 pKa = 9.12KK41 pKa = 10.53LSAA44 pKa = 4.03

Molecular weight: 5.44 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3963

0

3963

1224091

39

1766

308.9

34.4

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.11 ± 0.044

1.239 ± 0.016

5.218 ± 0.029

6.225 ± 0.04

4.291 ± 0.029

6.882 ± 0.037

1.902 ± 0.016

8.359 ± 0.034

6.962 ± 0.035

9.619 ± 0.044

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

3.008 ± 0.019

4.569 ± 0.028

3.396 ± 0.022

3.816 ± 0.025

3.744 ± 0.028

5.786 ± 0.031

5.513 ± 0.029

6.937 ± 0.035

0.91 ± 0.016

3.514 ± 0.026
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski