Wenling crustacean virus 1

Taxonomy: Viruses; Riboviria; unclassified Riboviria; unclassified RNA viruses ShiM-2016

Average proteome isoelectric point is 5.64

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1L3KKE8|A0A1L3KKE8_9VIRU Uncharacterized protein OS=Wenling crustacean virus 1 OX=1923478 PE=4 SV=1
MM1 pKa = 7.54PRR3 pKa = 11.84GRR5 pKa = 11.84NIATTGRR12 pKa = 11.84GQHH15 pKa = 6.01INLKK19 pKa = 9.98GVEE22 pKa = 4.65DD23 pKa = 4.48EE24 pKa = 6.21LPDD27 pKa = 3.49IDD29 pKa = 5.58NFFDD33 pKa = 4.33YY34 pKa = 10.77ISALYY39 pKa = 10.55RR40 pKa = 11.84NAQLLFSTHH49 pKa = 4.6TTFTDD54 pKa = 3.65EE55 pKa = 4.13VAANASLNSPITRR68 pKa = 11.84FLLVPGANSLPQPGAASAFTITLLPFRR95 pKa = 11.84TSEE98 pKa = 3.86MSLVVSDD105 pKa = 4.72ISDD108 pKa = 3.69ANISDD113 pKa = 3.77QFQCPISTLQEE124 pKa = 4.41DD125 pKa = 3.84SGGWQPFTTTRR136 pKa = 11.84QPIKK140 pKa = 10.2PIAIDD145 pKa = 3.1IDD147 pKa = 3.58RR148 pKa = 11.84GRR150 pKa = 11.84EE151 pKa = 3.78HH152 pKa = 7.26LLLNVEE158 pKa = 4.31IDD160 pKa = 3.2GLTYY164 pKa = 8.4TTEE167 pKa = 3.74LTLALNSFVYY177 pKa = 9.97IHH179 pKa = 6.16VEE181 pKa = 3.88SYY183 pKa = 10.81GFSVYY188 pKa = 8.65TQSPYY193 pKa = 11.32KK194 pKa = 10.36HH195 pKa = 6.54IEE197 pKa = 3.87LAFHH201 pKa = 6.61KK202 pKa = 9.53TMQIDD207 pKa = 3.99CAAPNPTSSIHH218 pKa = 6.09NSIGTYY224 pKa = 9.52TILTILHH231 pKa = 6.05TRR233 pKa = 11.84VEE235 pKa = 4.73SPGPDD240 pKa = 3.1YY241 pKa = 10.08TQRR244 pKa = 11.84FHH246 pKa = 7.6IGSTLPFAQIDD257 pKa = 3.57AHH259 pKa = 6.79DD260 pKa = 4.39ALLTVVKK267 pKa = 10.4AVARR271 pKa = 11.84HH272 pKa = 5.62SLDD275 pKa = 3.21DD276 pKa = 4.2HH277 pKa = 6.34YY278 pKa = 11.77AEE280 pKa = 5.14AMHH283 pKa = 7.17GDD285 pKa = 3.79STLLAPLATLTQSVLALTSRR305 pKa = 11.84VPMYY309 pKa = 10.4EE310 pKa = 3.79RR311 pKa = 11.84EE312 pKa = 4.24HH313 pKa = 6.4VQFTSGVNFVTAAEE327 pKa = 4.27RR328 pKa = 11.84LWSRR332 pKa = 11.84RR333 pKa = 11.84LEE335 pKa = 4.22SNPAGGMSSSYY346 pKa = 9.54VWPGLRR352 pKa = 11.84YY353 pKa = 8.81VGKK356 pKa = 9.19PVDD359 pKa = 3.46YY360 pKa = 10.41GATYY364 pKa = 10.46FSTTHH369 pKa = 6.18TSGSCQVSIGDD380 pKa = 3.82SSLHH384 pKa = 6.52PGHH387 pKa = 6.59SSHH390 pKa = 7.83DD391 pKa = 3.74EE392 pKa = 3.78VTLQFTYY399 pKa = 10.79DD400 pKa = 3.22QDD402 pKa = 4.68FDD404 pKa = 4.77DD405 pKa = 3.69SDD407 pKa = 3.85YY408 pKa = 10.82RR409 pKa = 11.84VYY411 pKa = 10.56RR412 pKa = 11.84ARR414 pKa = 11.84YY415 pKa = 6.94TNFRR419 pKa = 11.84QFGDD423 pKa = 3.79DD424 pKa = 3.15NFFNNTFPVYY434 pKa = 9.92TSGSFSTQSVSAMNSFSAVHH454 pKa = 5.76CQGTLTFIKK463 pKa = 10.82GNTTVEE469 pKa = 4.08KK470 pKa = 10.97DD471 pKa = 3.36FDD473 pKa = 3.91TTIPIQLVRR482 pKa = 11.84DD483 pKa = 3.28GDD485 pKa = 4.06TYY487 pKa = 10.83RR488 pKa = 11.84GNWVAQIGTVSLPRR502 pKa = 11.84SNFSLPSNDD511 pKa = 2.57MWFTCWTDD519 pKa = 3.22GTVEE523 pKa = 3.96HH524 pKa = 7.57DD525 pKa = 3.35NRR527 pKa = 11.84FPFITDD533 pKa = 3.1KK534 pKa = 11.42LDD536 pKa = 3.59FGLYY540 pKa = 9.3TDD542 pKa = 4.52PPRR545 pKa = 11.84LKK547 pKa = 10.31YY548 pKa = 10.17LAAIPLIFDD557 pKa = 4.39APDD560 pKa = 3.18EE561 pKa = 4.25LLAVSDD567 pKa = 3.86ILVTGALDD575 pKa = 3.94FSLDD579 pKa = 4.02LDD581 pKa = 4.54FAPQFQPQPPFISSTEE597 pKa = 3.84NALAALTLQNSIKK610 pKa = 10.6LSIIGMDD617 pKa = 3.61LTIIDD622 pKa = 3.87NRR624 pKa = 11.84IDD626 pKa = 3.4AMEE629 pKa = 4.89SNTRR633 pKa = 11.84PNIFQFLGGIALTASMFMPMGKK655 pKa = 10.08VMLATMLAGYY665 pKa = 9.49SLEE668 pKa = 4.38TVGDD672 pKa = 3.5VMANDD677 pKa = 4.57FVTIGANTLALVATLIAHH695 pKa = 6.42HH696 pKa = 6.62RR697 pKa = 11.84MSGGVFHH704 pKa = 7.54RR705 pKa = 11.84DD706 pKa = 3.22DD707 pKa = 3.8VLSTQLPFFEE717 pKa = 6.03HH718 pKa = 7.35PDD720 pKa = 3.26LPQIEE725 pKa = 4.51NTTRR729 pKa = 11.84FRR731 pKa = 11.84TLKK734 pKa = 10.49EE735 pKa = 3.9NASLNSDD742 pKa = 3.24QFGALTRR749 pKa = 11.84PLGDD753 pKa = 3.45TTFSEE758 pKa = 4.49PFVAVRR764 pKa = 11.84TSRR767 pKa = 11.84IGVLPTPDD775 pKa = 3.99HH776 pKa = 6.64SLLPASFVDD785 pKa = 4.29AAGLLEE791 pKa = 4.4KK792 pKa = 10.57RR793 pKa = 11.84SLYY796 pKa = 9.82PEE798 pKa = 3.83HH799 pKa = 6.77HH800 pKa = 6.16QLHH803 pKa = 6.5VNTLQLYY810 pKa = 5.84EE811 pKa = 4.2TEE813 pKa = 4.6GEE815 pKa = 4.38VKK817 pKa = 10.21AVNVLTVLGVSDD829 pKa = 4.52GPQRR833 pKa = 11.84FNGVFPSASGFGHH846 pKa = 6.28TPSEE850 pKa = 4.22PGVFTLLHH858 pKa = 6.45SGTSQSSVIPLEE870 pKa = 4.34SLSGATPEE878 pKa = 4.77QILRR882 pKa = 11.84DD883 pKa = 3.87YY884 pKa = 10.51RR885 pKa = 11.84AKK887 pKa = 10.42CLSIGKK893 pKa = 9.9SYY895 pKa = 11.01IDD897 pKa = 4.09TEE899 pKa = 4.19NADD902 pKa = 3.95LDD904 pKa = 4.16TLRR907 pKa = 11.84RR908 pKa = 11.84WYY910 pKa = 10.57LGSNPARR917 pKa = 11.84PLHH920 pKa = 5.21THH922 pKa = 6.94LPTFTGTAKK931 pKa = 10.29SAQVNIPIEE940 pKa = 4.2LVEE943 pKa = 4.53QITASMADD951 pKa = 3.32HH952 pKa = 6.51NAFGKK957 pKa = 9.32PYY959 pKa = 10.43KK960 pKa = 10.82YY961 pKa = 10.9NLTNNNCKK969 pKa = 10.21HH970 pKa = 6.0FADD973 pKa = 4.83SMFSMIAYY981 pKa = 9.7GEE983 pKa = 3.97RR984 pKa = 11.84DD985 pKa = 3.47EE986 pKa = 4.72MNAAFLSRR994 pKa = 11.84LSSTRR999 pKa = 11.84FNPGVITEE1007 pKa = 4.58FEE1009 pKa = 4.77LIQYY1013 pKa = 10.66LDD1015 pKa = 5.17DD1016 pKa = 4.56ININTINTVKK1026 pKa = 10.42PGGPIIYY1033 pKa = 8.95TRR1035 pKa = 11.84EE1036 pKa = 3.58GWLL1039 pKa = 3.39

Molecular weight: 114.79 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1L3KKE8|A0A1L3KKE8_9VIRU Uncharacterized protein OS=Wenling crustacean virus 1 OX=1923478 PE=4 SV=1
MM1 pKa = 7.64SEE3 pKa = 4.3PFQVSEE9 pKa = 4.22VTSAKK14 pKa = 8.95GTRR17 pKa = 11.84NAPGYY22 pKa = 8.47STLDD26 pKa = 3.51PQPVQVAKK34 pKa = 10.35PVTTTYY40 pKa = 10.81NYY42 pKa = 10.59RR43 pKa = 11.84FAAPNIVFRR52 pKa = 11.84GAPGTSVLNLRR63 pKa = 11.84LSLNHH68 pKa = 6.64LKK70 pKa = 10.93LSDD73 pKa = 3.7PVLAKK78 pKa = 10.63LATSYY83 pKa = 7.87QHH85 pKa = 6.09WRR87 pKa = 11.84INNMSVEE94 pKa = 4.42TKK96 pKa = 10.66AVGAMATSSGSFYY109 pKa = 10.53TSYY112 pKa = 10.9ISDD115 pKa = 4.1PLMVVPPDD123 pKa = 3.99PEE125 pKa = 5.61DD126 pKa = 3.56LLKK129 pKa = 11.23LLVRR133 pKa = 11.84RR134 pKa = 11.84PEE136 pKa = 4.38TEE138 pKa = 3.56QHH140 pKa = 5.25TARR143 pKa = 11.84QNDD146 pKa = 3.74LQKK149 pKa = 10.33IHH151 pKa = 7.28PLGTAPSFQKK161 pKa = 10.58KK162 pKa = 6.77YY163 pKa = 9.21TYY165 pKa = 10.12TSLGGDD171 pKa = 3.64PRR173 pKa = 11.84LSEE176 pKa = 3.94YY177 pKa = 9.33GTYY180 pKa = 10.07VMAMATAPGAADD192 pKa = 3.33QMLVTVTISLEE203 pKa = 4.0IEE205 pKa = 4.23FEE207 pKa = 4.44LPTIIPDD214 pKa = 3.57TMTLSTPDD222 pKa = 3.65IWTMAQDD229 pKa = 3.48PQYY232 pKa = 11.63NFGPLGAYY240 pKa = 9.45IEE242 pKa = 4.48IRR244 pKa = 11.84THH246 pKa = 6.11SLAVPQVAGRR256 pKa = 11.84FIATPPNTIQLLLSDD271 pKa = 4.5GDD273 pKa = 3.76VSEE276 pKa = 4.74LVVVTITEE284 pKa = 3.88CDD286 pKa = 3.39AVVDD290 pKa = 3.69SSGRR294 pKa = 11.84VTLSIPLPATSNAYY308 pKa = 10.42VDD310 pKa = 3.99MPDD313 pKa = 3.0TVTPVGDD320 pKa = 3.74LEE322 pKa = 4.26IYY324 pKa = 10.66GYY326 pKa = 10.53INYY329 pKa = 9.82SEE331 pKa = 4.69DD332 pKa = 3.29RR333 pKa = 11.84SAEE336 pKa = 4.12RR337 pKa = 11.84IRR339 pKa = 11.84GMISKK344 pKa = 10.1RR345 pKa = 11.84NQSTKK350 pKa = 10.33RR351 pKa = 11.84FASTLRR357 pKa = 11.84NVNLHH362 pKa = 6.77PKK364 pKa = 8.64TKK366 pKa = 8.78TAIISTHH373 pKa = 7.34AINKK377 pKa = 9.13HH378 pKa = 5.62PEE380 pKa = 3.97NGPAQPVRR388 pKa = 11.84PP389 pKa = 4.11

Molecular weight: 42.55 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3

0

3

3268

389

1840

1089.3

121.76

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.436 ± 0.172

1.561 ± 0.628

5.569 ± 0.396

5.906 ± 1.041

4.284 ± 0.771

6.028 ± 0.233

4.131 ± 0.793

5.936 ± 0.124

3.733 ± 0.771

9.027 ± 0.354

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.142 ± 0.178

4.345 ± 0.222

5.508 ± 0.953

3.856 ± 0.093

5.416 ± 0.485

6.916 ± 0.993

8.048 ± 0.93

5.845 ± 0.487

0.826 ± 0.097

3.488 ± 0.169
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski