Maize-associated totivirus 3
Average proteome isoelectric point is 6.54
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 3 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A2H5CRU9|A0A2H5CRU9_9VIRU RNA-directed RNA polymerase OS=Maize-associated totivirus 3 OX=2057198 PE=3 SV=1
MM1 pKa = 7.59 DD2 pKa = 3.86 TFVRR6 pKa = 11.84 HH7 pKa = 5.63 FFKK10 pKa = 10.78 EE11 pKa = 4.03 VTTPGVPFVLDD22 pKa = 4.12 EE23 pKa = 4.72 ACHH26 pKa = 5.28 TWTYY30 pKa = 10.21 FQGFKK35 pKa = 10.36 TVVTGDD41 pKa = 3.39 ASKK44 pKa = 11.15 SSARR48 pKa = 11.84 DD49 pKa = 3.04 IYY51 pKa = 11.26 QAMTPLGMLEE61 pKa = 4.1 TAFEE65 pKa = 4.71 SMGSNYY71 pKa = 10.7 DD72 pKa = 3.6 GLTKK76 pKa = 10.65 NYY78 pKa = 7.33 LTPEE82 pKa = 3.95 GAINFSNIHH91 pKa = 6.26 DD92 pKa = 4.01 TLKK95 pKa = 10.89 QSGGMNNQEE104 pKa = 4.83 LSTFMAFCQTISWHH118 pKa = 6.73 DD119 pKa = 3.44 NHH121 pKa = 5.78 VTLLFNMLRR130 pKa = 11.84 YY131 pKa = 9.76 VILMKK136 pKa = 10.81 FKK138 pKa = 10.51 EE139 pKa = 4.58 CKK141 pKa = 9.74 QNLNGALGRR150 pKa = 11.84 FQEE153 pKa = 5.08 DD154 pKa = 3.44 FLEE157 pKa = 5.15 INMDD161 pKa = 3.75 QYY163 pKa = 11.43 WPDD166 pKa = 3.29 SYY168 pKa = 11.53 PRR170 pKa = 11.84 DD171 pKa = 3.73 CAFDD175 pKa = 3.44 SWPVDD180 pKa = 3.49 VTEE183 pKa = 4.93 EE184 pKa = 3.84 GAIALEE190 pKa = 4.34 HH191 pKa = 6.89 LSTDD195 pKa = 3.33 QTRR198 pKa = 11.84 NTGPAIDD205 pKa = 4.48 LRR207 pKa = 11.84 GLTSTEE213 pKa = 4.21 SIFVIFMLHH222 pKa = 4.92 KK223 pKa = 7.64 WTRR226 pKa = 11.84 SSRR229 pKa = 11.84 FKK231 pKa = 10.65 IDD233 pKa = 3.03 VSTKK237 pKa = 10.64 KK238 pKa = 9.88 LTDD241 pKa = 3.22 TLQFRR246 pKa = 11.84 GVDD249 pKa = 3.35 VSALYY254 pKa = 10.46 DD255 pKa = 3.96 SYY257 pKa = 11.12 WKK259 pKa = 10.19 QDD261 pKa = 3.2 TVMPALLSSNAAWHH275 pKa = 6.52 ALAKK279 pKa = 10.59 YY280 pKa = 7.53 VTQNRR285 pKa = 11.84 LFDD288 pKa = 3.71 QFSSALYY295 pKa = 10.36 LVTALTYY302 pKa = 10.44 QYY304 pKa = 11.16 VPEE307 pKa = 4.25 TAEE310 pKa = 4.11 GVAWLSLNWRR320 pKa = 11.84 VVLPEE325 pKa = 3.81 FTSIRR330 pKa = 11.84 GKK332 pKa = 10.35 FAFFNTGDD340 pKa = 3.66 AAFLSQRR347 pKa = 11.84 SLDD350 pKa = 3.2 EE351 pKa = 3.74 WEE353 pKa = 4.42 YY354 pKa = 11.02 IGGKK358 pKa = 6.64 TEE360 pKa = 4.65 KK361 pKa = 10.07 INLMSLTFCQAFYY374 pKa = 11.16 AGIALRR380 pKa = 11.84 TTRR383 pKa = 11.84 RR384 pKa = 11.84 GLEE387 pKa = 3.85 MMPADD392 pKa = 4.17 IYY394 pKa = 11.06 GTDD397 pKa = 3.36 AEE399 pKa = 4.62 FWNAEE404 pKa = 4.15 NFVSAAVAEE413 pKa = 4.58 FARR416 pKa = 11.84 IKK418 pKa = 10.79 VPMSGTPGLYY428 pKa = 8.37 ITCDD432 pKa = 3.29 EE433 pKa = 4.75 RR434 pKa = 11.84 LDD436 pKa = 3.66 IQNDD440 pKa = 3.44 DD441 pKa = 4.08 RR442 pKa = 11.84 VVTTNNPNRR451 pKa = 11.84 RR452 pKa = 11.84 DD453 pKa = 3.65 LPPTHH458 pKa = 7.25 TYY460 pKa = 11.29 GEE462 pKa = 4.32 VQIEE466 pKa = 4.27 EE467 pKa = 4.41 TVLDD471 pKa = 5.36 DD472 pKa = 5.4 IEE474 pKa = 5.92 LDD476 pKa = 3.61 PTTLPDD482 pKa = 4.22 RR483 pKa = 11.84 DD484 pKa = 3.81 PDD486 pKa = 3.79 KK487 pKa = 10.82 QVHH490 pKa = 5.66 INRR493 pKa = 11.84 MRR495 pKa = 11.84 IAKK498 pKa = 9.84 VGAEE502 pKa = 4.51 AMRR505 pKa = 11.84 AMDD508 pKa = 4.47 PTCQLPAGFEE518 pKa = 3.71 LALRR522 pKa = 11.84 DD523 pKa = 3.36 RR524 pKa = 11.84 VAATRR529 pKa = 11.84 PRR531 pKa = 11.84 VRR533 pKa = 11.84 QAARR537 pKa = 11.84 VTVACPLLPFPGVPTLLLPTQCTPYY562 pKa = 10.35 PIPFEE567 pKa = 4.04 LAGKK571 pKa = 10.07 FDD573 pKa = 4.57 DD574 pKa = 4.27 SMGRR578 pKa = 11.84 FDD580 pKa = 5.53 RR581 pKa = 11.84 RR582 pKa = 11.84 GTRR585 pKa = 11.84 LKK587 pKa = 10.25 IDD589 pKa = 3.56 VAWRR593 pKa = 11.84 VMNFLQDD600 pKa = 4.1 QSYY603 pKa = 8.05 TWQCEE608 pKa = 3.87 YY609 pKa = 10.67 RR610 pKa = 11.84 GTRR613 pKa = 11.84 IGQQEE618 pKa = 4.44 YY619 pKa = 10.05 VRR621 pKa = 11.84 GRR623 pKa = 11.84 YY624 pKa = 9.93 SNMIWPVLYY633 pKa = 10.44 QPEE636 pKa = 4.71 DD637 pKa = 3.5 NDD639 pKa = 3.11 EE640 pKa = 4.07 DD641 pKa = 4.28 VILRR645 pKa = 11.84 RR646 pKa = 11.84 ITEE649 pKa = 3.87 VGLPDD654 pKa = 3.66 VPLPPMHH661 pKa = 7.07 NEE663 pKa = 3.66 FFKK666 pKa = 11.18 GKK668 pKa = 9.26 SVEE671 pKa = 4.05 YY672 pKa = 9.53 KK673 pKa = 8.65 YY674 pKa = 11.15 QSIRR678 pKa = 11.84 RR679 pKa = 11.84 GLAARR684 pKa = 11.84 FRR686 pKa = 11.84 GAKK689 pKa = 9.25 IDD691 pKa = 3.37 ISSYY695 pKa = 8.68 GAHH698 pKa = 5.66 VPVLKK703 pKa = 10.81 KK704 pKa = 10.19 MSTVTYY710 pKa = 10.32 AVNEE714 pKa = 4.09 GLRR717 pKa = 11.84 TVRR720 pKa = 11.84 GFIEE724 pKa = 4.44 RR725 pKa = 11.84 AAQDD729 pKa = 3.36 FRR731 pKa = 11.84 LAPVAGPGVIPPIEE745 pKa = 4.0 QAPDD749 pKa = 2.96 AA750 pKa = 4.84
Molecular weight: 85.15 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 5.406
IPC2_protein 5.448
IPC_protein 5.448
Toseland 5.601
ProMoST 5.677
Dawson 5.563
Bjellqvist 5.639
Wikipedia 5.499
Rodwell 5.499
Grimsley 5.639
Solomon 5.563
Lehninger 5.537
Nozaki 5.753
DTASelect 5.931
Thurlkill 5.741
EMBOSS 5.715
Sillero 5.83
Patrickios 4.126
IPC_peptide 5.575
IPC2_peptide 5.83
IPC2.peptide.svr19 5.812
Protein with the highest isoelectric point:
>tr|A0A2H5CRV4|A0A2H5CRV4_9VIRU RNA-directed RNA polymerase OS=Maize-associated totivirus 3 OX=2057198 PE=3 SV=1
MM1 pKa = 7.59 LNATPGGDD9 pKa = 3.14 GPYY12 pKa = 9.82 IRR14 pKa = 11.84 RR15 pKa = 11.84 KK16 pKa = 10.19 EE17 pKa = 3.95 PLTLNNVPVWVNIRR31 pKa = 11.84 RR32 pKa = 11.84 NHH34 pKa = 4.77 LTAVDD39 pKa = 3.36 RR40 pKa = 11.84 HH41 pKa = 5.68 DD42 pKa = 4.12 AKK44 pKa = 10.88 HH45 pKa = 5.39 VLGFILPGEE54 pKa = 4.25 SFDD57 pKa = 3.43 VGGFGRR63 pKa = 11.84 YY64 pKa = 8.23 LVGKK68 pKa = 9.76 ACVAAIAIPCDD79 pKa = 3.99 GMWLLYY85 pKa = 10.92 VSVTTPANILPPIVKK100 pKa = 8.62 RR101 pKa = 11.84 TLSAMFSTVDD111 pKa = 3.28 GYY113 pKa = 11.61 YY114 pKa = 10.44 FHH116 pKa = 7.91 DD117 pKa = 4.89 ADD119 pKa = 3.74 TTKK122 pKa = 10.84 YY123 pKa = 10.0 LRR125 pKa = 11.84 KK126 pKa = 10.05 AFDD129 pKa = 3.17 VDD131 pKa = 3.54 RR132 pKa = 11.84 SVIAHH137 pKa = 6.86 AAKK140 pKa = 10.01 PIPPHH145 pKa = 5.69 EE146 pKa = 4.72 GEE148 pKa = 4.26 FEE150 pKa = 4.09 RR151 pKa = 11.84 AVITAEE157 pKa = 3.76 HH158 pKa = 6.0 HH159 pKa = 3.99 THH161 pKa = 5.67 YY162 pKa = 10.47 RR163 pKa = 11.84 PEE165 pKa = 4.46 EE166 pKa = 3.34 IWAIAKK172 pKa = 7.32 QHH174 pKa = 5.48 KK175 pKa = 6.06 TTLKK179 pKa = 10.91 AMGIVLANLRR189 pKa = 11.84 KK190 pKa = 9.22 IQGVTEE196 pKa = 4.01 ATVATFLAYY205 pKa = 9.84 IMTVRR210 pKa = 11.84 PQVAYY215 pKa = 11.1 LMATSKK221 pKa = 10.9 RR222 pKa = 11.84 IWRR225 pKa = 11.84 SKK227 pKa = 10.76 NIAEE231 pKa = 4.1 LTEE234 pKa = 3.96 VLKK237 pKa = 11.4 NIATPIKK244 pKa = 10.56 SMHH247 pKa = 4.83 QHH249 pKa = 6.84 EE250 pKa = 4.73 ICDD253 pKa = 3.69 LTEE256 pKa = 4.52 LFEE259 pKa = 4.38 LQCLVNRR266 pKa = 11.84 GVGQIDD272 pKa = 3.16 WRR274 pKa = 11.84 KK275 pKa = 8.48 EE276 pKa = 3.62 RR277 pKa = 11.84 SHH279 pKa = 6.02 RR280 pKa = 11.84 TSPDD284 pKa = 3.13 VVKK287 pKa = 11.09 VSLADD292 pKa = 3.44 VVKK295 pKa = 10.19 YY296 pKa = 9.26 ATEE299 pKa = 3.76 IFLLGKK305 pKa = 9.98 AHH307 pKa = 7.11 GYY309 pKa = 10.07 HH310 pKa = 5.45 YY311 pKa = 11.15 RR312 pKa = 11.84 KK313 pKa = 8.94 MDD315 pKa = 3.23 KK316 pKa = 10.2 QKK318 pKa = 10.82 YY319 pKa = 9.23 ISARR323 pKa = 11.84 WEE325 pKa = 3.68 WSPTGSIHH333 pKa = 5.96 SQYY336 pKa = 10.82 PEE338 pKa = 4.76 DD339 pKa = 4.67 DD340 pKa = 3.87 PYY342 pKa = 11.23 IPGNYY347 pKa = 8.03 RR348 pKa = 11.84 QKK350 pKa = 10.02 TKK352 pKa = 10.49 FVALNMMSRR361 pKa = 11.84 QHH363 pKa = 6.35 VDD365 pKa = 2.47 SMFLRR370 pKa = 11.84 KK371 pKa = 9.52 PEE373 pKa = 3.62 IHH375 pKa = 6.53 AWTSVKK381 pKa = 10.73 YY382 pKa = 9.62 EE383 pKa = 3.87 WAKK386 pKa = 10.25 QRR388 pKa = 11.84 AIYY391 pKa = 10.09 GVDD394 pKa = 3.3 LTSTVITNFAMYY406 pKa = 10.0 RR407 pKa = 11.84 CEE409 pKa = 4.02 EE410 pKa = 4.23 VFKK413 pKa = 11.11 HH414 pKa = 5.86 RR415 pKa = 11.84 FPVGEE420 pKa = 3.83 EE421 pKa = 3.42 AAADD425 pKa = 3.84 RR426 pKa = 11.84 VHH428 pKa = 6.86 RR429 pKa = 11.84 RR430 pKa = 11.84 LKK432 pKa = 10.67 IMLEE436 pKa = 4.24 DD437 pKa = 4.03 NEE439 pKa = 4.66 SFCYY443 pKa = 10.81 DD444 pKa = 3.25 FDD446 pKa = 5.14 DD447 pKa = 5.86 FNAQHH452 pKa = 6.09 STQAMQAVLVAYY464 pKa = 10.27 LNVFQADD471 pKa = 3.46 MSDD474 pKa = 3.73 DD475 pKa = 3.63 QRR477 pKa = 11.84 DD478 pKa = 3.53 AMSWVIDD485 pKa = 3.75 SLSDD489 pKa = 3.6 VTIHH493 pKa = 6.46 NSNVQPPEE501 pKa = 3.75 QYY503 pKa = 9.89 EE504 pKa = 4.25 LKK506 pKa = 9.94 GTLLSGWRR514 pKa = 11.84 LTTFMNTALNYY525 pKa = 10.15 VYY527 pKa = 10.1 FKK529 pKa = 11.06 AAGCFDD535 pKa = 3.23 IGGVRR540 pKa = 11.84 DD541 pKa = 3.92 SVHH544 pKa = 6.66 NGDD547 pKa = 5.02 DD548 pKa = 3.41 VLVSIKK554 pKa = 10.63 HH555 pKa = 5.58 IGAAVKK561 pKa = 9.64 IHH563 pKa = 6.24 HH564 pKa = 6.72 RR565 pKa = 11.84 MALINARR572 pKa = 11.84 AQPTKK577 pKa = 10.89 CNVFSVGEE585 pKa = 3.91 FLRR588 pKa = 11.84 VEE590 pKa = 4.23 HH591 pKa = 7.01 KK592 pKa = 10.46 VDD594 pKa = 3.32 KK595 pKa = 10.22 DD596 pKa = 3.38 TGLGAQYY603 pKa = 9.02 LTRR606 pKa = 11.84 ACATLVHH613 pKa = 6.2 SRR615 pKa = 11.84 CEE617 pKa = 4.19 SQEE620 pKa = 4.0 PEE622 pKa = 4.31 HH623 pKa = 6.18 LTGAVKK629 pKa = 10.65 AIVTRR634 pKa = 11.84 ARR636 pKa = 11.84 EE637 pKa = 3.86 VFEE640 pKa = 4.02 RR641 pKa = 11.84 AKK643 pKa = 10.44 ISQALLADD651 pKa = 4.33 LVRR654 pKa = 11.84 SAIRR658 pKa = 11.84 RR659 pKa = 11.84 VAAVFHH665 pKa = 6.74 RR666 pKa = 11.84 PAKK669 pKa = 8.7 EE670 pKa = 3.65 AFVIAEE676 pKa = 3.91 LHH678 pKa = 5.85 AVVGGASTEE687 pKa = 4.05 DD688 pKa = 3.59 FAPIDD693 pKa = 3.6 FKK695 pKa = 11.3 INEE698 pKa = 4.09 RR699 pKa = 11.84 CEE701 pKa = 3.92 YY702 pKa = 9.15 SQEE705 pKa = 3.87 RR706 pKa = 11.84 SASDD710 pKa = 3.39 KK711 pKa = 11.41 DD712 pKa = 4.15 DD713 pKa = 3.54 MVVTQDD719 pKa = 4.31 LYY721 pKa = 10.84 PGIFDD726 pKa = 3.83 YY727 pKa = 11.32 ARR729 pKa = 11.84 TLAQAYY735 pKa = 9.23 EE736 pKa = 4.37 GVLDD740 pKa = 3.81 EE741 pKa = 4.31 QQAKK745 pKa = 9.91 RR746 pKa = 11.84 RR747 pKa = 11.84 VISATTRR754 pKa = 11.84 QISVTRR760 pKa = 11.84 KK761 pKa = 6.37 TWLDD765 pKa = 3.55 IVPLPADD772 pKa = 3.35 TFYY775 pKa = 11.27 RR776 pKa = 11.84 FGRR779 pKa = 11.84 ALYY782 pKa = 10.5 KK783 pKa = 9.7 MYY785 pKa = 10.33 HH786 pKa = 6.28 GLVSMPHH793 pKa = 6.11 LEE795 pKa = 3.84 KK796 pKa = 10.88 ARR798 pKa = 11.84 FVGIPPIALLDD809 pKa = 3.65 SRR811 pKa = 11.84 SRR813 pKa = 11.84 NIIRR817 pKa = 11.84 NIIVEE822 pKa = 4.16 ASDD825 pKa = 3.14 VEE827 pKa = 4.31 YY828 pKa = 10.99 ALRR831 pKa = 11.84 VLLL834 pKa = 4.86
Molecular weight: 94.54 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 7.632
IPC2_protein 7.936
IPC_protein 7.775
Toseland 7.732
ProMoST 8.331
Dawson 8.551
Bjellqvist 8.741
Wikipedia 8.536
Rodwell 8.595
Grimsley 7.834
Solomon 8.609
Lehninger 8.609
Nozaki 8.843
DTASelect 8.551
Thurlkill 8.609
EMBOSS 8.726
Sillero 8.873
Patrickios 4.444
IPC_peptide 8.609
IPC2_peptide 7.614
IPC2.peptide.svr19 7.779
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
3
0
3
3192
750
1608
1064.0
120.71
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
8.897 ± 0.416
1.472 ± 0.084
6.234 ± 0.166
5.482 ± 0.01
4.699 ± 0.345
5.388 ± 0.207
2.82 ± 0.479
5.42 ± 0.309
4.574 ± 0.334
7.926 ± 0.18
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.757 ± 0.117
3.509 ± 0.148
4.699 ± 0.383
3.697 ± 0.166
7.018 ± 0.081
5.294 ± 0.092
6.892 ± 0.395
7.675 ± 0.413
1.723 ± 0.084
3.822 ± 0.043
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here