Maize-associated totivirus 3

Taxonomy: Viruses; Riboviria; Orthornavirae; Duplornaviricota; Chrymotiviricetes; Ghabrivirales; Totiviridae; unclassified Totiviridae

Average proteome isoelectric point is 6.54

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    

Protein with the lowest isoelectric point:
>tr|A0A2H5CRU9|A0A2H5CRU9_9VIRU RNA-directed RNA polymerase OS=Maize-associated totivirus 3 OX=2057198 PE=3 SV=1
MM1 pKa = 7.59DD2 pKa = 3.86TFVRR6 pKa = 11.84HH7 pKa = 5.63FFKK10 pKa = 10.78EE11 pKa = 4.03VTTPGVPFVLDD22 pKa = 4.12EE23 pKa = 4.72ACHH26 pKa = 5.28TWTYY30 pKa = 10.21FQGFKK35 pKa = 10.36TVVTGDD41 pKa = 3.39ASKK44 pKa = 11.15SSARR48 pKa = 11.84DD49 pKa = 3.04IYY51 pKa = 11.26QAMTPLGMLEE61 pKa = 4.1TAFEE65 pKa = 4.71SMGSNYY71 pKa = 10.7DD72 pKa = 3.6GLTKK76 pKa = 10.65NYY78 pKa = 7.33LTPEE82 pKa = 3.95GAINFSNIHH91 pKa = 6.26DD92 pKa = 4.01TLKK95 pKa = 10.89QSGGMNNQEE104 pKa = 4.83LSTFMAFCQTISWHH118 pKa = 6.73DD119 pKa = 3.44NHH121 pKa = 5.78VTLLFNMLRR130 pKa = 11.84YY131 pKa = 9.76VILMKK136 pKa = 10.81FKK138 pKa = 10.51EE139 pKa = 4.58CKK141 pKa = 9.74QNLNGALGRR150 pKa = 11.84FQEE153 pKa = 5.08DD154 pKa = 3.44FLEE157 pKa = 5.15INMDD161 pKa = 3.75QYY163 pKa = 11.43WPDD166 pKa = 3.29SYY168 pKa = 11.53PRR170 pKa = 11.84DD171 pKa = 3.73CAFDD175 pKa = 3.44SWPVDD180 pKa = 3.49VTEE183 pKa = 4.93EE184 pKa = 3.84GAIALEE190 pKa = 4.34HH191 pKa = 6.89LSTDD195 pKa = 3.33QTRR198 pKa = 11.84NTGPAIDD205 pKa = 4.48LRR207 pKa = 11.84GLTSTEE213 pKa = 4.21SIFVIFMLHH222 pKa = 4.92KK223 pKa = 7.64WTRR226 pKa = 11.84SSRR229 pKa = 11.84FKK231 pKa = 10.65IDD233 pKa = 3.03VSTKK237 pKa = 10.64KK238 pKa = 9.88LTDD241 pKa = 3.22TLQFRR246 pKa = 11.84GVDD249 pKa = 3.35VSALYY254 pKa = 10.46DD255 pKa = 3.96SYY257 pKa = 11.12WKK259 pKa = 10.19QDD261 pKa = 3.2TVMPALLSSNAAWHH275 pKa = 6.52ALAKK279 pKa = 10.59YY280 pKa = 7.53VTQNRR285 pKa = 11.84LFDD288 pKa = 3.71QFSSALYY295 pKa = 10.36LVTALTYY302 pKa = 10.44QYY304 pKa = 11.16VPEE307 pKa = 4.25TAEE310 pKa = 4.11GVAWLSLNWRR320 pKa = 11.84VVLPEE325 pKa = 3.81FTSIRR330 pKa = 11.84GKK332 pKa = 10.35FAFFNTGDD340 pKa = 3.66AAFLSQRR347 pKa = 11.84SLDD350 pKa = 3.2EE351 pKa = 3.74WEE353 pKa = 4.42YY354 pKa = 11.02IGGKK358 pKa = 6.64TEE360 pKa = 4.65KK361 pKa = 10.07INLMSLTFCQAFYY374 pKa = 11.16AGIALRR380 pKa = 11.84TTRR383 pKa = 11.84RR384 pKa = 11.84GLEE387 pKa = 3.85MMPADD392 pKa = 4.17IYY394 pKa = 11.06GTDD397 pKa = 3.36AEE399 pKa = 4.62FWNAEE404 pKa = 4.15NFVSAAVAEE413 pKa = 4.58FARR416 pKa = 11.84IKK418 pKa = 10.79VPMSGTPGLYY428 pKa = 8.37ITCDD432 pKa = 3.29EE433 pKa = 4.75RR434 pKa = 11.84LDD436 pKa = 3.66IQNDD440 pKa = 3.44DD441 pKa = 4.08RR442 pKa = 11.84VVTTNNPNRR451 pKa = 11.84RR452 pKa = 11.84DD453 pKa = 3.65LPPTHH458 pKa = 7.25TYY460 pKa = 11.29GEE462 pKa = 4.32VQIEE466 pKa = 4.27EE467 pKa = 4.41TVLDD471 pKa = 5.36DD472 pKa = 5.4IEE474 pKa = 5.92LDD476 pKa = 3.61PTTLPDD482 pKa = 4.22RR483 pKa = 11.84DD484 pKa = 3.81PDD486 pKa = 3.79KK487 pKa = 10.82QVHH490 pKa = 5.66INRR493 pKa = 11.84MRR495 pKa = 11.84IAKK498 pKa = 9.84VGAEE502 pKa = 4.51AMRR505 pKa = 11.84AMDD508 pKa = 4.47PTCQLPAGFEE518 pKa = 3.71LALRR522 pKa = 11.84DD523 pKa = 3.36RR524 pKa = 11.84VAATRR529 pKa = 11.84PRR531 pKa = 11.84VRR533 pKa = 11.84QAARR537 pKa = 11.84VTVACPLLPFPGVPTLLLPTQCTPYY562 pKa = 10.35PIPFEE567 pKa = 4.04LAGKK571 pKa = 10.07FDD573 pKa = 4.57DD574 pKa = 4.27SMGRR578 pKa = 11.84FDD580 pKa = 5.53RR581 pKa = 11.84RR582 pKa = 11.84GTRR585 pKa = 11.84LKK587 pKa = 10.25IDD589 pKa = 3.56VAWRR593 pKa = 11.84VMNFLQDD600 pKa = 4.1QSYY603 pKa = 8.05TWQCEE608 pKa = 3.87YY609 pKa = 10.67RR610 pKa = 11.84GTRR613 pKa = 11.84IGQQEE618 pKa = 4.44YY619 pKa = 10.05VRR621 pKa = 11.84GRR623 pKa = 11.84YY624 pKa = 9.93SNMIWPVLYY633 pKa = 10.44QPEE636 pKa = 4.71DD637 pKa = 3.5NDD639 pKa = 3.11EE640 pKa = 4.07DD641 pKa = 4.28VILRR645 pKa = 11.84RR646 pKa = 11.84ITEE649 pKa = 3.87VGLPDD654 pKa = 3.66VPLPPMHH661 pKa = 7.07NEE663 pKa = 3.66FFKK666 pKa = 11.18GKK668 pKa = 9.26SVEE671 pKa = 4.05YY672 pKa = 9.53KK673 pKa = 8.65YY674 pKa = 11.15QSIRR678 pKa = 11.84RR679 pKa = 11.84GLAARR684 pKa = 11.84FRR686 pKa = 11.84GAKK689 pKa = 9.25IDD691 pKa = 3.37ISSYY695 pKa = 8.68GAHH698 pKa = 5.66VPVLKK703 pKa = 10.81KK704 pKa = 10.19MSTVTYY710 pKa = 10.32AVNEE714 pKa = 4.09GLRR717 pKa = 11.84TVRR720 pKa = 11.84GFIEE724 pKa = 4.44RR725 pKa = 11.84AAQDD729 pKa = 3.36FRR731 pKa = 11.84LAPVAGPGVIPPIEE745 pKa = 4.0QAPDD749 pKa = 2.96AA750 pKa = 4.84

Molecular weight:
85.15 kDa
Isoelectric point according different methods:






Protein with the highest isoelectric point:
>tr|A0A2H5CRV4|A0A2H5CRV4_9VIRU RNA-directed RNA polymerase OS=Maize-associated totivirus 3 OX=2057198 PE=3 SV=1
MM1 pKa = 7.59LNATPGGDD9 pKa = 3.14GPYY12 pKa = 9.82IRR14 pKa = 11.84RR15 pKa = 11.84KK16 pKa = 10.19EE17 pKa = 3.95PLTLNNVPVWVNIRR31 pKa = 11.84RR32 pKa = 11.84NHH34 pKa = 4.77LTAVDD39 pKa = 3.36RR40 pKa = 11.84HH41 pKa = 5.68DD42 pKa = 4.12AKK44 pKa = 10.88HH45 pKa = 5.39VLGFILPGEE54 pKa = 4.25SFDD57 pKa = 3.43VGGFGRR63 pKa = 11.84YY64 pKa = 8.23LVGKK68 pKa = 9.76ACVAAIAIPCDD79 pKa = 3.99GMWLLYY85 pKa = 10.92VSVTTPANILPPIVKK100 pKa = 8.62RR101 pKa = 11.84TLSAMFSTVDD111 pKa = 3.28GYY113 pKa = 11.61YY114 pKa = 10.44FHH116 pKa = 7.91DD117 pKa = 4.89ADD119 pKa = 3.74TTKK122 pKa = 10.84YY123 pKa = 10.0LRR125 pKa = 11.84KK126 pKa = 10.05AFDD129 pKa = 3.17VDD131 pKa = 3.54RR132 pKa = 11.84SVIAHH137 pKa = 6.86AAKK140 pKa = 10.01PIPPHH145 pKa = 5.69EE146 pKa = 4.72GEE148 pKa = 4.26FEE150 pKa = 4.09RR151 pKa = 11.84AVITAEE157 pKa = 3.76HH158 pKa = 6.0HH159 pKa = 3.99THH161 pKa = 5.67YY162 pKa = 10.47RR163 pKa = 11.84PEE165 pKa = 4.46EE166 pKa = 3.34IWAIAKK172 pKa = 7.32QHH174 pKa = 5.48KK175 pKa = 6.06TTLKK179 pKa = 10.91AMGIVLANLRR189 pKa = 11.84KK190 pKa = 9.22IQGVTEE196 pKa = 4.01ATVATFLAYY205 pKa = 9.84IMTVRR210 pKa = 11.84PQVAYY215 pKa = 11.1LMATSKK221 pKa = 10.9RR222 pKa = 11.84IWRR225 pKa = 11.84SKK227 pKa = 10.76NIAEE231 pKa = 4.1LTEE234 pKa = 3.96VLKK237 pKa = 11.4NIATPIKK244 pKa = 10.56SMHH247 pKa = 4.83QHH249 pKa = 6.84EE250 pKa = 4.73ICDD253 pKa = 3.69LTEE256 pKa = 4.52LFEE259 pKa = 4.38LQCLVNRR266 pKa = 11.84GVGQIDD272 pKa = 3.16WRR274 pKa = 11.84KK275 pKa = 8.48EE276 pKa = 3.62RR277 pKa = 11.84SHH279 pKa = 6.02RR280 pKa = 11.84TSPDD284 pKa = 3.13VVKK287 pKa = 11.09VSLADD292 pKa = 3.44VVKK295 pKa = 10.19YY296 pKa = 9.26ATEE299 pKa = 3.76IFLLGKK305 pKa = 9.98AHH307 pKa = 7.11GYY309 pKa = 10.07HH310 pKa = 5.45YY311 pKa = 11.15RR312 pKa = 11.84KK313 pKa = 8.94MDD315 pKa = 3.23KK316 pKa = 10.2QKK318 pKa = 10.82YY319 pKa = 9.23ISARR323 pKa = 11.84WEE325 pKa = 3.68WSPTGSIHH333 pKa = 5.96SQYY336 pKa = 10.82PEE338 pKa = 4.76DD339 pKa = 4.67DD340 pKa = 3.87PYY342 pKa = 11.23IPGNYY347 pKa = 8.03RR348 pKa = 11.84QKK350 pKa = 10.02TKK352 pKa = 10.49FVALNMMSRR361 pKa = 11.84QHH363 pKa = 6.35VDD365 pKa = 2.47SMFLRR370 pKa = 11.84KK371 pKa = 9.52PEE373 pKa = 3.62IHH375 pKa = 6.53AWTSVKK381 pKa = 10.73YY382 pKa = 9.62EE383 pKa = 3.87WAKK386 pKa = 10.25QRR388 pKa = 11.84AIYY391 pKa = 10.09GVDD394 pKa = 3.3LTSTVITNFAMYY406 pKa = 10.0RR407 pKa = 11.84CEE409 pKa = 4.02EE410 pKa = 4.23VFKK413 pKa = 11.11HH414 pKa = 5.86RR415 pKa = 11.84FPVGEE420 pKa = 3.83EE421 pKa = 3.42AAADD425 pKa = 3.84RR426 pKa = 11.84VHH428 pKa = 6.86RR429 pKa = 11.84RR430 pKa = 11.84LKK432 pKa = 10.67IMLEE436 pKa = 4.24DD437 pKa = 4.03NEE439 pKa = 4.66SFCYY443 pKa = 10.81DD444 pKa = 3.25FDD446 pKa = 5.14DD447 pKa = 5.86FNAQHH452 pKa = 6.09STQAMQAVLVAYY464 pKa = 10.27LNVFQADD471 pKa = 3.46MSDD474 pKa = 3.73DD475 pKa = 3.63QRR477 pKa = 11.84DD478 pKa = 3.53AMSWVIDD485 pKa = 3.75SLSDD489 pKa = 3.6VTIHH493 pKa = 6.46NSNVQPPEE501 pKa = 3.75QYY503 pKa = 9.89EE504 pKa = 4.25LKK506 pKa = 9.94GTLLSGWRR514 pKa = 11.84LTTFMNTALNYY525 pKa = 10.15VYY527 pKa = 10.1FKK529 pKa = 11.06AAGCFDD535 pKa = 3.23IGGVRR540 pKa = 11.84DD541 pKa = 3.92SVHH544 pKa = 6.66NGDD547 pKa = 5.02DD548 pKa = 3.41VLVSIKK554 pKa = 10.63HH555 pKa = 5.58IGAAVKK561 pKa = 9.64IHH563 pKa = 6.24HH564 pKa = 6.72RR565 pKa = 11.84MALINARR572 pKa = 11.84AQPTKK577 pKa = 10.89CNVFSVGEE585 pKa = 3.91FLRR588 pKa = 11.84VEE590 pKa = 4.23HH591 pKa = 7.01KK592 pKa = 10.46VDD594 pKa = 3.32KK595 pKa = 10.22DD596 pKa = 3.38TGLGAQYY603 pKa = 9.02LTRR606 pKa = 11.84ACATLVHH613 pKa = 6.2SRR615 pKa = 11.84CEE617 pKa = 4.19SQEE620 pKa = 4.0PEE622 pKa = 4.31HH623 pKa = 6.18LTGAVKK629 pKa = 10.65AIVTRR634 pKa = 11.84ARR636 pKa = 11.84EE637 pKa = 3.86VFEE640 pKa = 4.02RR641 pKa = 11.84AKK643 pKa = 10.44ISQALLADD651 pKa = 4.33LVRR654 pKa = 11.84SAIRR658 pKa = 11.84RR659 pKa = 11.84VAAVFHH665 pKa = 6.74RR666 pKa = 11.84PAKK669 pKa = 8.7EE670 pKa = 3.65AFVIAEE676 pKa = 3.91LHH678 pKa = 5.85AVVGGASTEE687 pKa = 4.05DD688 pKa = 3.59FAPIDD693 pKa = 3.6FKK695 pKa = 11.3INEE698 pKa = 4.09RR699 pKa = 11.84CEE701 pKa = 3.92YY702 pKa = 9.15SQEE705 pKa = 3.87RR706 pKa = 11.84SASDD710 pKa = 3.39KK711 pKa = 11.41DD712 pKa = 4.15DD713 pKa = 3.54MVVTQDD719 pKa = 4.31LYY721 pKa = 10.84PGIFDD726 pKa = 3.83YY727 pKa = 11.32ARR729 pKa = 11.84TLAQAYY735 pKa = 9.23EE736 pKa = 4.37GVLDD740 pKa = 3.81EE741 pKa = 4.31QQAKK745 pKa = 9.91RR746 pKa = 11.84RR747 pKa = 11.84VISATTRR754 pKa = 11.84QISVTRR760 pKa = 11.84KK761 pKa = 6.37TWLDD765 pKa = 3.55IVPLPADD772 pKa = 3.35TFYY775 pKa = 11.27RR776 pKa = 11.84FGRR779 pKa = 11.84ALYY782 pKa = 10.5KK783 pKa = 9.7MYY785 pKa = 10.33HH786 pKa = 6.28GLVSMPHH793 pKa = 6.11LEE795 pKa = 3.84KK796 pKa = 10.88ARR798 pKa = 11.84FVGIPPIALLDD809 pKa = 3.65SRR811 pKa = 11.84SRR813 pKa = 11.84NIIRR817 pKa = 11.84NIIVEE822 pKa = 4.16ASDD825 pKa = 3.14VEE827 pKa = 4.31YY828 pKa = 10.99ALRR831 pKa = 11.84VLLL834 pKa = 4.86

Molecular weight:
94.54 kDa
Isoelectric point according different methods:






Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3

0

3

3192

750

1608

1064.0

120.71

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.897 ± 0.416

1.472 ± 0.084

6.234 ± 0.166

5.482 ± 0.01

4.699 ± 0.345

5.388 ± 0.207

2.82 ± 0.479

5.42 ± 0.309

4.574 ± 0.334

7.926 ± 0.18

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.757 ± 0.117

3.509 ± 0.148

4.699 ± 0.383

3.697 ± 0.166

7.018 ± 0.081

5.294 ± 0.092

6.892 ± 0.395

7.675 ± 0.413

1.723 ± 0.084

3.822 ± 0.043

Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski