Alkalihalobacillus lonarensis

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Bacilli; Bacillales; Bacillaceae; Alkalihalobacillus

Average proteome isoelectric point is 6.32

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2581 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1G6JIM0|A0A1G6JIM0_9BACI RNA polymerase sigma factor OS=Alkalihalobacillus lonarensis OX=1464122 GN=SAMN05421737_1065 PE=3 SV=1
MM1 pKa = 7.26SQPNMPNFTPNITLDD16 pKa = 3.51RR17 pKa = 11.84DD18 pKa = 3.92DD19 pKa = 4.35VVNLLLASIAMEE31 pKa = 4.19EE32 pKa = 4.16MGLAHH37 pKa = 7.7IINAEE42 pKa = 4.05GEE44 pKa = 4.34KK45 pKa = 9.94IQYY48 pKa = 11.05ALGTLNGLTGPPATLQEE65 pKa = 3.76ILAINEE71 pKa = 4.11SAADD75 pKa = 3.65MLDD78 pKa = 3.52TIFKK82 pKa = 10.57KK83 pKa = 10.62EE84 pKa = 4.04IILEE88 pKa = 4.29SKK90 pKa = 9.82MKK92 pKa = 10.38AATNIPTLIGPTGATGATGPAGSVTNINGIFPDD125 pKa = 4.02GAGTVTITIGDD136 pKa = 3.63IPGGIEE142 pKa = 4.17NINGITAAPDD152 pKa = 3.48GSLTLDD158 pKa = 3.97AEE160 pKa = 4.66DD161 pKa = 3.89VGAFPYY167 pKa = 10.85EE168 pKa = 4.19EE169 pKa = 4.44VPSGSDD175 pKa = 2.97LDD177 pKa = 4.22SFITEE182 pKa = 4.15GVYY185 pKa = 10.55SSEE188 pKa = 4.14GNAGGPVNGPLGFTSPIWTLYY209 pKa = 10.54VSVITPGPTTSILQLLIGNPTIYY232 pKa = 10.33VRR234 pKa = 11.84SATDD238 pKa = 3.26DD239 pKa = 3.43TGTTWNPWEE248 pKa = 4.1EE249 pKa = 4.84LGTQGPTGPTGAGVTGATGATGATGATGATGATGVTGVTGVTGATGVTGATGATGVTGVTGATGVTGATGATGATGVTGVTGATGVTGATGATGVTGATGVTGATGVTGPTGVTGATGPTGPTGPTGSTGPTGATGPTGPTGPTGSTGPTGPTGVTGPTGVTGPTGPTGVTGSTGVTGITGVTGPTGPTGVTGPTGPTGVTGATGVTGVTGATGPTGSTGPTGATGPTGPDD480 pKa = 3.14PTVNEE485 pKa = 4.07VFISNSGQHH494 pKa = 5.37SVYY497 pKa = 10.38IIDD500 pKa = 5.59GNTQDD505 pKa = 4.1ILATIDD511 pKa = 3.28TTGTDD516 pKa = 3.15TRR518 pKa = 11.84GLVVNPNTNFLYY530 pKa = 10.55AINQGSSNVSVIDD543 pKa = 3.83LTTQAISTIISVAANPYY560 pKa = 10.08GLGINVDD567 pKa = 3.72TNLLYY572 pKa = 9.63VTHH575 pKa = 7.4RR576 pKa = 11.84APTNQVSVIEE586 pKa = 4.42GLNHH590 pKa = 5.22TVVTAFNIGSDD601 pKa = 3.55PVANPTVDD609 pKa = 3.3PNTNLVYY616 pKa = 10.57CANNVSNNVTVIDD629 pKa = 4.72GLTQTITATVTVGTNPFRR647 pKa = 11.84IVANPSTNQVYY658 pKa = 10.04VSNQGSDD665 pKa = 3.03NVSVINATNQTLMPTIPLAGGAAPQGITVNPNTNLIYY702 pKa = 10.81VVATNLQSVLVVDD715 pKa = 5.61WITLDD720 pKa = 3.89TIATIAVGTGAYY732 pKa = 7.47TAGANPRR739 pKa = 11.84TNLIYY744 pKa = 9.24VTKK747 pKa = 10.69VADD750 pKa = 3.77NNISVIDD757 pKa = 4.56GINNNIIATITSALPATGFRR777 pKa = 11.84SVSVLEE783 pKa = 4.06GLRR786 pKa = 11.84LTGPLYY792 pKa = 10.94SS793 pKa = 3.95

Molecular weight: 77.55 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1G6NC61|A0A1G6NC61_9BACI Acetyltransferase (GNAT) domain-containing protein OS=Alkalihalobacillus lonarensis OX=1464122 GN=SAMN05421737_11232 PE=4 SV=1
MM1 pKa = 7.65PNISRR6 pKa = 11.84IQALCRR12 pKa = 11.84MMMGLTLLAWAAARR26 pKa = 11.84FTRR29 pKa = 11.84RR30 pKa = 11.84PYY32 pKa = 10.85CPFSFIMALFGAQKK46 pKa = 8.89TAEE49 pKa = 4.13GTLRR53 pKa = 11.84FCPLVALRR61 pKa = 11.84KK62 pKa = 9.25RR63 pKa = 11.84CYY65 pKa = 10.33EE66 pKa = 3.84RR67 pKa = 11.84DD68 pKa = 3.31

Molecular weight: 7.86 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2581

0

2581

755743

29

4605

292.8

32.7

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.285 ± 0.059

0.886 ± 0.018

5.187 ± 0.042

7.228 ± 0.061

4.223 ± 0.045

7.028 ± 0.064

2.513 ± 0.029

6.314 ± 0.043

5.659 ± 0.052

9.778 ± 0.06

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.966 ± 0.027

3.324 ± 0.035

3.843 ± 0.051

4.011 ± 0.04

4.873 ± 0.045

5.429 ± 0.035

6.137 ± 0.065

7.891 ± 0.051

1.074 ± 0.021

3.35 ± 0.031
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski