Sulfolobales archaeon SCGC AB-777_K09

Taxonomy: cellular organisms; Archaea; TACK group; Crenarchaeota; Thermoprotei; Sulfolobales; unclassified Sulfolobales

Average proteome isoelectric point is 6.91

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 206 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A2T9WN64|A0A2T9WN64_9CREN Redox-regulated ATPase YchF (Fragment) OS=Sulfolobales archaeon SCGC AB-777_K09 OX=2005260 GN=ychF PE=4 SV=1
MM1 pKa = 7.78PPDD4 pKa = 3.39QQGNSYY10 pKa = 9.47YY11 pKa = 10.46SQYY14 pKa = 11.15GVFWLYY20 pKa = 11.32NWFQADD26 pKa = 4.04NNNMSIFFLMNGNIFNYY43 pKa = 9.69SLSYY47 pKa = 10.4PFIGYY52 pKa = 9.04AIPPNPPSSTINGEE66 pKa = 4.12PIFAQSYY73 pKa = 8.08IDD75 pKa = 4.03AYY77 pKa = 9.77NTAFNQEE84 pKa = 3.73TSRR87 pKa = 11.84YY88 pKa = 7.68NLNYY92 pKa = 9.61QVALNKK98 pKa = 9.59YY99 pKa = 10.17RR100 pKa = 11.84KK101 pKa = 8.79FAYY104 pKa = 10.4DD105 pKa = 3.4NVNSNFVPGTFGYY118 pKa = 10.04QEE120 pKa = 4.44EE121 pKa = 4.75YY122 pKa = 10.82NDD124 pKa = 4.04RR125 pKa = 11.84SNPSNSIIINYY136 pKa = 8.88LQDD139 pKa = 3.57DD140 pKa = 3.94ANYY143 pKa = 10.55YY144 pKa = 10.61DD145 pKa = 3.83GNPYY149 pKa = 10.72LFISAGSGGGSGFMYY164 pKa = 10.49LDD166 pKa = 3.58WVIVTYY172 pKa = 9.46GVPYY176 pKa = 9.98VVSVSS181 pKa = 3.23

Molecular weight: 20.7 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A2T9WNS1|A0A2T9WNS1_9CREN Beta-CASP ribonuclease aCPSF1 OS=Sulfolobales archaeon SCGC AB-777_K09 OX=2005260 GN=DDW04_00325 PE=4 SV=1
MM1 pKa = 7.48TSRR4 pKa = 11.84KK5 pKa = 8.94ARR7 pKa = 11.84NYY9 pKa = 9.3ARR11 pKa = 11.84KK12 pKa = 9.59KK13 pKa = 8.25VTEE16 pKa = 4.18LLSLAEE22 pKa = 4.18ILVKK26 pKa = 10.58NDD28 pKa = 3.23PEE30 pKa = 4.29TAKK33 pKa = 10.54EE34 pKa = 3.97VAEE37 pKa = 4.14LAFITSRR44 pKa = 11.84KK45 pKa = 9.3LRR47 pKa = 11.84VSIPRR52 pKa = 11.84KK53 pKa = 8.21VKK55 pKa = 9.95RR56 pKa = 11.84RR57 pKa = 11.84FCRR60 pKa = 11.84RR61 pKa = 11.84CHH63 pKa = 6.1VPLIPGVTMRR73 pKa = 11.84IRR75 pKa = 11.84IKK77 pKa = 10.66RR78 pKa = 11.84KK79 pKa = 7.16TLVVTCLTCGWIRR92 pKa = 11.84RR93 pKa = 11.84YY94 pKa = 9.5EE95 pKa = 3.96LSRR98 pKa = 11.84GVDD101 pKa = 2.91KK102 pKa = 11.66GEE104 pKa = 3.78NN105 pKa = 3.33

Molecular weight: 12.22 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

206

0

206

55869

37

1486

271.2

30.45

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.92 ± 0.178

0.616 ± 0.059

4.908 ± 0.174

7.815 ± 0.258

3.773 ± 0.11

6.753 ± 0.13

1.351 ± 0.059

6.941 ± 0.155

6.986 ± 0.227

10.283 ± 0.207

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.155 ± 0.088

3.87 ± 0.191

4.29 ± 0.125

2.191 ± 0.117

5.869 ± 0.218

6.207 ± 0.162

4.972 ± 0.237

9.077 ± 0.161

0.94 ± 0.056

4.083 ± 0.142
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski