Sulfitobacter sp. S4J41

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Alphaproteobacteria; Rhodobacterales; Rhodobacteraceae; Antarcticimicrobium

Average proteome isoelectric point is 6.26

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4217 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A4R5F0K9|A0A4R5F0K9_9RHOB Glycosyltransferase family 2 protein OS=Sulfitobacter sp. S4J41 OX=2546227 GN=E1B25_00805 PE=4 SV=1
MM1 pKa = 7.59SKK3 pKa = 8.77TLTAALALGAPVLFSASDD21 pKa = 3.47VLAQEE26 pKa = 4.51SRR28 pKa = 11.84RR29 pKa = 11.84FTWDD33 pKa = 2.92GEE35 pKa = 4.1IEE37 pKa = 3.88IDD39 pKa = 3.66YY40 pKa = 11.13EE41 pKa = 4.98SIFDD45 pKa = 3.72SDD47 pKa = 3.87VPANEE52 pKa = 4.17SDD54 pKa = 3.25TAYY57 pKa = 10.75GSGEE61 pKa = 3.97FNAAYY66 pKa = 10.57ALTDD70 pKa = 3.43RR71 pKa = 11.84VAIFGGLTFEE81 pKa = 4.38EE82 pKa = 4.93LEE84 pKa = 4.22NAAGNTGYY92 pKa = 11.1GFYY95 pKa = 10.47FHH97 pKa = 7.4EE98 pKa = 5.73LGLQFEE104 pKa = 4.66TGAASFQIGKK114 pKa = 7.75VHH116 pKa = 7.06PEE118 pKa = 4.26FGTAWDD124 pKa = 4.06TAAGFYY130 pKa = 10.82GSALAEE136 pKa = 4.52DD137 pKa = 4.84YY138 pKa = 10.56EE139 pKa = 4.7LSEE142 pKa = 4.31QIGVLAEE149 pKa = 4.49LDD151 pKa = 3.63LGTAGTLAMGVFYY164 pKa = 10.94ADD166 pKa = 3.66DD167 pKa = 3.91TGLSDD172 pKa = 4.0SLGFNRR178 pKa = 11.84GRR180 pKa = 11.84NTTAAGGAGNTGTLNNASLQWSKK203 pKa = 11.61DD204 pKa = 2.96WGQTFAYY211 pKa = 9.86VGARR215 pKa = 11.84FLTAGTGDD223 pKa = 3.38VSDD226 pKa = 3.7EE227 pKa = 4.15TGIVAGVGHH236 pKa = 6.38SLGNGLDD243 pKa = 3.7LFGEE247 pKa = 4.6VAAFDD252 pKa = 4.83GYY254 pKa = 11.27GGSADD259 pKa = 3.75DD260 pKa = 3.83ATYY263 pKa = 8.77ATLNAAYY270 pKa = 9.95AIGEE274 pKa = 4.28LTLSGTYY281 pKa = 11.04AMRR284 pKa = 11.84DD285 pKa = 3.04VDD287 pKa = 4.07SAGRR291 pKa = 11.84TDD293 pKa = 4.42LVSLAAEE300 pKa = 4.21YY301 pKa = 10.85EE302 pKa = 4.36LTNGMTLGGALARR315 pKa = 11.84VDD317 pKa = 3.82DD318 pKa = 5.08AGVADD323 pKa = 4.09TLFGVNLVIPFGGG336 pKa = 3.3

Molecular weight: 34.75 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A4V2Z8A9|A0A4V2Z8A9_9RHOB TIGR02302 family protein OS=Sulfitobacter sp. S4J41 OX=2546227 GN=E1B25_06230 PE=4 SV=1
MM1 pKa = 7.45KK2 pKa = 9.61RR3 pKa = 11.84TFQPSNLVRR12 pKa = 11.84KK13 pKa = 9.18RR14 pKa = 11.84RR15 pKa = 11.84HH16 pKa = 4.42GFRR19 pKa = 11.84ARR21 pKa = 11.84MATKK25 pKa = 10.37AGRR28 pKa = 11.84KK29 pKa = 8.54ILNARR34 pKa = 11.84RR35 pKa = 11.84ARR37 pKa = 11.84GRR39 pKa = 11.84KK40 pKa = 9.07SLSAA44 pKa = 3.93

Molecular weight: 5.14 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4217

0

4217

1348334

22

2210

319.7

34.66

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

12.384 ± 0.049

0.898 ± 0.011

5.972 ± 0.035

5.711 ± 0.031

3.647 ± 0.021

8.783 ± 0.035

2.047 ± 0.016

5.107 ± 0.024

3.129 ± 0.03

10.311 ± 0.048

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.825 ± 0.018

2.464 ± 0.017

5.19 ± 0.028

3.259 ± 0.021

6.816 ± 0.037

5.16 ± 0.027

5.476 ± 0.025

7.241 ± 0.029

1.36 ± 0.016

2.219 ± 0.019
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski