Micromonas pusilla virus PL1

Taxonomy: Viruses; Varidnaviria; Bamfordvirae; Nucleocytoviricota; Megaviricetes; Algavirales; Phycodnaviridae; Prasinovirus; Micromonas pusilla virus SP1 sensu lato

Average proteome isoelectric point is 6.6

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 259 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|G8DDE4|G8DDE4_9PHYC Uncharacterized protein OS=Micromonas pusilla virus PL1 OX=373997 GN=MPWG_00163 PE=4 SV=1
MM1 pKa = 7.57NMQTGFGDD9 pKa = 4.18AGDD12 pKa = 4.17TQATNYY18 pKa = 8.03MNTMIDD24 pKa = 2.42IMMPVMEE31 pKa = 5.16KK32 pKa = 11.14SMILAAEE39 pKa = 4.17YY40 pKa = 10.7CKK42 pKa = 10.95ACGRR46 pKa = 11.84DD47 pKa = 3.49VILPEE52 pKa = 4.23DD53 pKa = 3.41MEE55 pKa = 4.55YY56 pKa = 11.12ASKK59 pKa = 10.22YY60 pKa = 9.83CAMNTVGQTIGSIFPEE76 pKa = 4.47VYY78 pKa = 10.35DD79 pKa = 4.62DD80 pKa = 5.32EE81 pKa = 4.87EE82 pKa = 6.06SEE84 pKa = 4.8DD85 pKa = 3.63EE86 pKa = 4.98DD87 pKa = 4.33EE88 pKa = 4.99IEE90 pKa = 4.5EE91 pKa = 4.27VAEE94 pKa = 4.33DD95 pKa = 3.68EE96 pKa = 4.82CPTFVRR102 pKa = 11.84YY103 pKa = 9.95SGSDD107 pKa = 3.11PKK109 pKa = 10.32FIQMNEE115 pKa = 3.9AYY117 pKa = 10.04DD118 pKa = 3.81RR119 pKa = 11.84WHH121 pKa = 5.67SWEE124 pKa = 4.1PQSPAEE130 pKa = 4.19QMLKK134 pKa = 10.5NALNSNEE141 pKa = 3.93HH142 pKa = 5.93MGAA145 pKa = 3.4

Molecular weight: 16.44 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|G8DDD3|G8DDD3_9PHYC Uncharacterized protein OS=Micromonas pusilla virus PL1 OX=373997 GN=MPWG_00152 PE=4 SV=1
MM1 pKa = 7.19VVRR4 pKa = 11.84IPLSNSGILSAHH16 pKa = 6.38GYY18 pKa = 9.14EE19 pKa = 4.5GVRR22 pKa = 11.84EE23 pKa = 3.93KK24 pKa = 11.32SEE26 pKa = 4.17LARR29 pKa = 11.84HH30 pKa = 5.63RR31 pKa = 11.84ALMRR35 pKa = 11.84VVRR38 pKa = 11.84AGEE41 pKa = 3.95PPLGLFRR48 pKa = 11.84RR49 pKa = 11.84LNVLMILFKK58 pKa = 10.98RR59 pKa = 11.84KK60 pKa = 9.72DD61 pKa = 3.52PKK63 pKa = 10.31LSKK66 pKa = 10.18IFKK69 pKa = 9.87EE70 pKa = 3.79DD71 pKa = 3.7RR72 pKa = 11.84DD73 pKa = 3.79WVRR76 pKa = 11.84EE77 pKa = 3.83KK78 pKa = 11.22LLL80 pKa = 3.82

Molecular weight: 9.34 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

259

0

259

60315

51

1699

232.9

26.3

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.446 ± 0.163

1.542 ± 0.115

5.723 ± 0.113

6.539 ± 0.196

4.341 ± 0.104

6.793 ± 0.245

2.034 ± 0.078

6.549 ± 0.147

7.174 ± 0.301

7.97 ± 0.143

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.787 ± 0.134

5.728 ± 0.216

4.193 ± 0.129

3.19 ± 0.077

4.702 ± 0.142

6.556 ± 0.159

6.68 ± 0.249

6.886 ± 0.166

1.159 ± 0.057

4.009 ± 0.112
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski