Halogeometricum borinquense (strain ATCC 700274 / DSM 11551 / JCM 10706 / KCTC 4070 / PR3)

Taxonomy: cellular organisms; Archaea; Euryarchaeota; Stenosarchaea group; Halobacteria; Haloferacales; Haloferacaceae; Halogeometricum; Halogeometricum borinquense

Average proteome isoelectric point is 4.99

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3894 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|E4NLU9|E4NLU9_HALBP V-type ATP synthase subunit C OS=Halogeometricum borinquense (strain ATCC 700274 / DSM 11551 / JCM 10706 / KCTC 4070 / PR3) OX=469382 GN=atpC PE=3 SV=1
MM1 pKa = 7.6GLTEE5 pKa = 4.14TLDD8 pKa = 4.08DD9 pKa = 3.98YY10 pKa = 11.87FDD12 pKa = 3.91VQEE15 pKa = 4.88HH16 pKa = 6.16GSSVGTEE23 pKa = 3.53IVAGVTTFLTMSYY36 pKa = 9.62IVVVNPSILTDD47 pKa = 3.31QPFIEE52 pKa = 5.03GVDD55 pKa = 4.57GIAIAGFSPAEE66 pKa = 4.09VQSMLAVVTIVAAAVATFVMAIYY89 pKa = 10.71ANRR92 pKa = 11.84PFGQAPGLGLNAFFAFTVVGALGVPWQTALAAVVAEE128 pKa = 5.02GILFILLTAVGARR141 pKa = 11.84EE142 pKa = 3.72YY143 pKa = 10.82VIRR146 pKa = 11.84LFPEE150 pKa = 4.02PVKK153 pKa = 10.47FAVGTGIGLFLTIIGLQAMGLVVNDD178 pKa = 3.64SATLITLGPVASDD191 pKa = 3.35PVAILSVVGLFFTFALYY208 pKa = 10.75ARR210 pKa = 11.84GIPGSIIIGILGTAFLGWVLTVAGIVTPDD239 pKa = 3.12AGLVADD245 pKa = 5.07ASSMTLSLPVLGTFDD260 pKa = 3.73VLVPAAGASATYY272 pKa = 10.71NVTPLVGAFVGGLGNVEE289 pKa = 4.2AFSFALIVFTFFFVDD304 pKa = 4.8FFDD307 pKa = 3.79TAGTLVGVSQIAGFLDD323 pKa = 4.42DD324 pKa = 6.14DD325 pKa = 5.08GNLPDD330 pKa = 4.08IDD332 pKa = 4.47KK333 pKa = 10.7PLMADD338 pKa = 4.29AIGTTVGGMIGTSTVTTYY356 pKa = 10.82VEE358 pKa = 4.2SASGVEE364 pKa = 3.93EE365 pKa = 4.24GGRR368 pKa = 11.84TGLTALTVSVLFLVSLAFVPVAAAIPQYY396 pKa = 10.46ASHH399 pKa = 7.01IALVVIGVVMLGNVIDD415 pKa = 4.68IDD417 pKa = 3.57WSDD420 pKa = 3.64VTNTIPAGMTILVMPFTYY438 pKa = 10.44SIAYY442 pKa = 8.97GIAAGIVSYY451 pKa = 10.36PLVKK455 pKa = 9.66TAGGEE460 pKa = 4.01FDD462 pKa = 4.86DD463 pKa = 4.92VRR465 pKa = 11.84PGHH468 pKa = 6.59WALALAFVVYY478 pKa = 8.68FFVRR482 pKa = 11.84TSGIIQAQVV491 pKa = 2.47

Molecular weight: 50.54 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|E4NLG2|E4NLG2_HALBP Histidine--tRNA ligase OS=Halogeometricum borinquense (strain ATCC 700274 / DSM 11551 / JCM 10706 / KCTC 4070 / PR3) OX=469382 GN=hisS PE=3 SV=1
MM1 pKa = 7.26PTNDD5 pKa = 3.46AATKK9 pKa = 9.09RR10 pKa = 11.84TLEE13 pKa = 3.84KK14 pKa = 10.14QICMRR19 pKa = 11.84CNARR23 pKa = 11.84NPARR27 pKa = 11.84ATSCRR32 pKa = 11.84KK33 pKa = 9.62CGYY36 pKa = 10.22KK37 pKa = 10.11NLRR40 pKa = 11.84PKK42 pKa = 10.56SKK44 pKa = 10.01EE45 pKa = 3.37RR46 pKa = 11.84RR47 pKa = 11.84AAA49 pKa = 3.42

Molecular weight: 5.6 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3894

0

3894

1130016

26

2192

290.2

31.65

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

10.204 ± 0.051

0.7 ± 0.013

8.155 ± 0.056

8.341 ± 0.056

3.475 ± 0.026

8.143 ± 0.039

2.026 ± 0.022

4.267 ± 0.031

2.255 ± 0.026

8.718 ± 0.05

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.812 ± 0.018

2.714 ± 0.025

4.49 ± 0.028

2.676 ± 0.025

6.325 ± 0.041

6.167 ± 0.039

6.746 ± 0.042

8.929 ± 0.046

1.129 ± 0.015

2.726 ± 0.021
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski