Paraoerskovia marina
Taxonomy:
Average proteome isoelectric point is 5.76
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 2637 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A1H1NHU9|A0A1H1NHU9_9CELL ATP-dependent helicase HrpA OS=Paraoerskovia marina OX=545619 GN=SAMN04489860_0540 PE=4 SV=1
MM1 pKa = 7.21 HH2 pKa = 7.98 RR3 pKa = 11.84 SPTILPDD10 pKa = 3.64 AQPPRR15 pKa = 11.84 ARR17 pKa = 11.84 HH18 pKa = 4.81 GVALVAATALLALAGTTTLAAVTLDD43 pKa = 3.99 ADD45 pKa = 3.91 PAAAAGTCEE54 pKa = 5.29 ADD56 pKa = 3.79 FDD58 pKa = 6.07 VNNQWNGGFGTNVSVTSPGDD78 pKa = 3.41 PVTSWEE84 pKa = 4.4 VTWEE88 pKa = 4.04 FSGDD92 pKa = 3.65 QQVTQLWNGTVSQSGNTVHH111 pKa = 6.63 VASLPWNGAVGTGGTLSFGFNGSGDD136 pKa = 3.64 GSTATPTAVTLNGAACSTGTDD157 pKa = 3.69 PTPTPTTTTPTPTPTEE173 pKa = 4.16 TTPTPTPTEE182 pKa = 4.23 TTDD185 pKa = 3.23 PPAADD190 pKa = 3.33 GDD192 pKa = 4.58 LYY194 pKa = 11.64 VDD196 pKa = 4.45 TGNQAYY202 pKa = 9.43 AAWSAASGDD211 pKa = 4.13 DD212 pKa = 3.33 KK213 pKa = 11.9 ALLAKK218 pKa = 10.24 IALTPQAYY226 pKa = 7.69 WVGNWDD232 pKa = 4.34 DD233 pKa = 4.36 PEE235 pKa = 4.42 HH236 pKa = 6.28 AQAEE240 pKa = 4.57 VQDD243 pKa = 3.95 YY244 pKa = 9.46 TGRR247 pKa = 11.84 AVAAGQVPQLVIYY260 pKa = 8.6 AIPGRR265 pKa = 11.84 DD266 pKa = 3.3 CGSYY270 pKa = 10.22 SGGGVAEE277 pKa = 4.34 SEE279 pKa = 4.2 YY280 pKa = 11.37 ADD282 pKa = 4.59 WIDD285 pKa = 3.39 TMAAGIQGNPLVVLEE300 pKa = 4.69 PDD302 pKa = 3.57 ALAQLGDD309 pKa = 4.21 CDD311 pKa = 4.1 GQGDD315 pKa = 4.01 RR316 pKa = 11.84 VGYY319 pKa = 9.38 LAYY322 pKa = 10.05 AASSLTDD329 pKa = 3.48 AGAHH333 pKa = 5.21 VYY335 pKa = 10.11 IDD337 pKa = 4.21 AGHH340 pKa = 6.29 SGWLTPATAADD351 pKa = 4.0 RR352 pKa = 11.84 LLQVGLDD359 pKa = 3.79 DD360 pKa = 4.65 AVGFAVNTSNYY371 pKa = 7.05 QTTADD376 pKa = 3.56 SQAWAEE382 pKa = 4.09 QVSALTGGAGYY393 pKa = 10.87 VIDD396 pKa = 4.08 TSRR399 pKa = 11.84 NGNGSNGQWCNPSGRR414 pKa = 11.84 ALGDD418 pKa = 3.43 RR419 pKa = 11.84 PSLVDD424 pKa = 5.11 DD425 pKa = 4.11 STNLDD430 pKa = 3.09 ALLWVKK436 pKa = 10.69 LPGEE440 pKa = 4.21 SDD442 pKa = 3.59 GTCNGGPSAGAWWQSMALEE461 pKa = 4.5 LARR464 pKa = 11.84 NASWW468 pKa = 3.09
Molecular weight: 47.86 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.707
IPC2_protein 3.783
IPC_protein 3.821
Toseland 3.579
ProMoST 3.999
Dawson 3.821
Bjellqvist 3.973
Wikipedia 3.783
Rodwell 3.643
Grimsley 3.49
Solomon 3.821
Lehninger 3.783
Nozaki 3.935
DTASelect 4.228
Thurlkill 3.643
EMBOSS 3.783
Sillero 3.948
Patrickios 0.846
IPC_peptide 3.808
IPC2_peptide 3.923
IPC2.peptide.svr19 3.833
Protein with the highest isoelectric point:
>tr|A0A1H1VMW4|A0A1H1VMW4_9CELL DNA-binding transcriptional regulator LacI/PurR family OS=Paraoerskovia marina OX=545619 GN=SAMN04489860_2553 PE=4 SV=1
MM1 pKa = 7.4 GSVIKK6 pKa = 10.42 KK7 pKa = 8.47 RR8 pKa = 11.84 RR9 pKa = 11.84 KK10 pKa = 9.22 RR11 pKa = 11.84 MAKK14 pKa = 9.41 KK15 pKa = 9.87 KK16 pKa = 9.7 HH17 pKa = 5.84 RR18 pKa = 11.84 KK19 pKa = 8.56 LLRR22 pKa = 11.84 KK23 pKa = 7.78 TRR25 pKa = 11.84 HH26 pKa = 3.65 QRR28 pKa = 11.84 RR29 pKa = 11.84 NKK31 pKa = 9.78 KK32 pKa = 9.85
Molecular weight: 4.08 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.493
IPC2_protein 11.14
IPC_protein 12.691
Toseland 12.866
ProMoST 13.349
Dawson 12.866
Bjellqvist 12.852
Wikipedia 13.334
Rodwell 12.735
Grimsley 12.91
Solomon 13.349
Lehninger 13.261
Nozaki 12.866
DTASelect 12.852
Thurlkill 12.866
EMBOSS 13.364
Sillero 12.866
Patrickios 12.457
IPC_peptide 13.349
IPC2_peptide 12.34
IPC2.peptide.svr19 9.071
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
2637
0
2637
905901
25
4501
343.5
36.57
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
13.289 ± 0.079
0.612 ± 0.012
6.976 ± 0.056
5.933 ± 0.043
2.666 ± 0.027
9.189 ± 0.042
1.986 ± 0.026
3.486 ± 0.037
1.635 ± 0.036
9.706 ± 0.056
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.697 ± 0.022
1.743 ± 0.028
5.619 ± 0.041
2.56 ± 0.025
7.293 ± 0.073
5.572 ± 0.033
6.59 ± 0.06
10.025 ± 0.054
1.503 ± 0.022
1.917 ± 0.023
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here