Microbacterium phage Rollins

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Armstrongvirus; unclassified Armstrongvirus

Average proteome isoelectric point is 6.22

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 68 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A3G2KJA8|A0A3G2KJA8_9CAUD Uncharacterized protein OS=Microbacterium phage Rollins OX=2419975 GN=46 PE=4 SV=1
MM1 pKa = 7.46NKK3 pKa = 9.42INHH6 pKa = 6.37NDD8 pKa = 3.18SLDD11 pKa = 4.14FINSVEE17 pKa = 4.48HH18 pKa = 6.71IAHH21 pKa = 6.54HH22 pKa = 5.52MTTEE26 pKa = 4.03EE27 pKa = 4.02LQEE30 pKa = 4.22LDD32 pKa = 3.44STKK35 pKa = 10.32IALQAIEE42 pKa = 4.5TVDD45 pKa = 3.45NCVDD49 pKa = 4.67AIRR52 pKa = 11.84FIEE55 pKa = 4.29LAITAHH61 pKa = 6.62GYY63 pKa = 7.52EE64 pKa = 3.47IRR66 pKa = 11.84YY67 pKa = 8.92IVAEE71 pKa = 4.84GYY73 pKa = 10.57DD74 pKa = 3.48YY75 pKa = 11.62GQFEE79 pKa = 4.61VLSDD83 pKa = 4.08LEE85 pKa = 4.56HH86 pKa = 7.13ALEE89 pKa = 4.58MIRR92 pKa = 11.84EE93 pKa = 4.22DD94 pKa = 4.44LLEE97 pKa = 4.38DD98 pKa = 3.37

Molecular weight: 11.29 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A3G2KJ09|A0A3G2KJ09_9CAUD Uncharacterized protein OS=Microbacterium phage Rollins OX=2419975 GN=22 PE=4 SV=1
MM1 pKa = 7.55KK2 pKa = 10.43CQVCKK7 pKa = 10.36RR8 pKa = 11.84RR9 pKa = 11.84QATHH13 pKa = 6.35VIRR16 pKa = 11.84NAFASSANGKK26 pKa = 9.36RR27 pKa = 11.84ICSNTEE33 pKa = 3.44CFRR36 pKa = 11.84RR37 pKa = 11.84PTGGYY42 pKa = 8.04PAEE45 pKa = 4.16ARR47 pKa = 11.84LIRR50 pKa = 11.84GG51 pKa = 3.45

Molecular weight: 5.71 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

68

0

68

12575

45

823

184.9

20.18

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

11.547 ± 0.426

0.477 ± 0.105

6.505 ± 0.255

6.052 ± 0.267

3.459 ± 0.178

8.62 ± 0.327

1.964 ± 0.217

4.644 ± 0.27

4.247 ± 0.256

8.549 ± 0.289

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.099 ± 0.131

3.586 ± 0.258

5.113 ± 0.226

3.459 ± 0.133

6.139 ± 0.333

5.32 ± 0.303

6.632 ± 0.235

7.117 ± 0.26

1.909 ± 0.149

2.561 ± 0.2
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski