Amino acid dipepetide frequency for Microbacterium phage Rollins

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
13.202AlaAla: 13.202 ± 1.408
0.318AlaCys: 0.318 ± 0.179
8.112AlaAsp: 8.112 ± 0.951
7.158AlaGlu: 7.158 ± 0.771
3.499AlaPhe: 3.499 ± 0.412
12.407AlaGly: 12.407 ± 0.79
1.75AlaHis: 1.75 ± 0.389
6.601AlaIle: 6.601 ± 1.141
5.726AlaLys: 5.726 ± 0.868
9.623AlaLeu: 9.623 ± 0.903
2.306AlaMet: 2.306 ± 0.442
3.261AlaAsn: 3.261 ± 0.476
4.772AlaPro: 4.772 ± 0.481
4.692AlaGln: 4.692 ± 0.61
5.806AlaArg: 5.806 ± 0.585
5.169AlaSer: 5.169 ± 0.716
8.192AlaThr: 8.192 ± 0.958
7.873AlaVal: 7.873 ± 0.851
2.227AlaTrp: 2.227 ± 0.393
2.704AlaTyr: 2.704 ± 0.507
0.0AlaXaa: 0.0 ± 0.0
Cys
0.318CysAla: 0.318 ± 0.164
0.0CysCys: 0.0 ± 0.0
0.08CysAsp: 0.08 ± 0.09
0.08CysGlu: 0.08 ± 0.075
0.477CysPhe: 0.477 ± 0.19
0.477CysGly: 0.477 ± 0.189
0.08CysHis: 0.08 ± 0.086
0.08CysIle: 0.08 ± 0.078
0.318CysLys: 0.318 ± 0.149
0.08CysLeu: 0.08 ± 0.085
0.08CysMet: 0.08 ± 0.081
0.159CysAsn: 0.159 ± 0.11
0.716CysPro: 0.716 ± 0.288
0.159CysGln: 0.159 ± 0.108
0.398CysArg: 0.398 ± 0.216
0.398CysSer: 0.398 ± 0.17
0.239CysThr: 0.239 ± 0.159
0.318CysVal: 0.318 ± 0.168
0.08CysTrp: 0.08 ± 0.075
0.239CysTyr: 0.239 ± 0.136
0.0CysXaa: 0.0 ± 0.0
Asp
7.078AspAla: 7.078 ± 0.634
0.08AspCys: 0.08 ± 0.087
3.579AspAsp: 3.579 ± 0.546
3.897AspGlu: 3.897 ± 0.577
3.42AspPhe: 3.42 ± 0.442
6.521AspGly: 6.521 ± 0.629
1.909AspHis: 1.909 ± 0.56
2.704AspIle: 2.704 ± 0.468
1.591AspLys: 1.591 ± 0.297
7.476AspLeu: 7.476 ± 0.745
1.193AspMet: 1.193 ± 0.299
1.909AspAsn: 1.909 ± 0.4
4.136AspPro: 4.136 ± 0.648
1.67AspGln: 1.67 ± 0.298
3.181AspArg: 3.181 ± 0.482
3.976AspSer: 3.976 ± 0.589
3.181AspThr: 3.181 ± 0.548
3.976AspVal: 3.976 ± 0.562
0.875AspTrp: 0.875 ± 0.24
2.704AspTyr: 2.704 ± 0.396
0.0AspXaa: 0.0 ± 0.0
Glu
7.237GluAla: 7.237 ± 0.734
0.239GluCys: 0.239 ± 0.14
4.454GluAsp: 4.454 ± 0.627
4.374GluGlu: 4.374 ± 0.557
2.943GluPhe: 2.943 ± 0.461
4.692GluGly: 4.692 ± 0.687
1.272GluHis: 1.272 ± 0.341
0.875GluIle: 0.875 ± 0.25
1.988GluLys: 1.988 ± 0.376
7.317GluLeu: 7.317 ± 0.676
1.034GluMet: 1.034 ± 0.21
1.988GluAsn: 1.988 ± 0.394
2.784GluPro: 2.784 ± 0.509
2.465GluGln: 2.465 ± 0.359
2.943GluArg: 2.943 ± 0.529
2.147GluSer: 2.147 ± 0.408
3.261GluThr: 3.261 ± 0.443
5.965GluVal: 5.965 ± 0.695
1.511GluTrp: 1.511 ± 0.363
1.034GluTyr: 1.034 ± 0.307
0.0GluXaa: 0.0 ± 0.0
Phe
2.943PheAla: 2.943 ± 0.384
0.0PheCys: 0.0 ± 0.0
2.068PheAsp: 2.068 ± 0.433
2.147PheGlu: 2.147 ± 0.288
1.034PhePhe: 1.034 ± 0.441
2.704PheGly: 2.704 ± 0.73
0.557PheHis: 0.557 ± 0.187
1.67PheIle: 1.67 ± 0.433
1.272PheLys: 1.272 ± 0.227
2.863PheLeu: 2.863 ± 0.505
1.113PheMet: 1.113 ± 0.294
1.511PheAsn: 1.511 ± 0.359
1.909PhePro: 1.909 ± 0.4
1.193PheGln: 1.193 ± 0.301
3.102PheArg: 3.102 ± 0.525
2.386PheSer: 2.386 ± 0.372
2.704PheThr: 2.704 ± 0.486
2.227PheVal: 2.227 ± 0.306
0.477PheTrp: 0.477 ± 0.192
0.716PheTyr: 0.716 ± 0.215
0.0PheXaa: 0.0 ± 0.0
Gly
8.351GlyAla: 8.351 ± 0.971
0.716GlyCys: 0.716 ± 0.287
6.68GlyAsp: 6.68 ± 0.659
5.249GlyGlu: 5.249 ± 0.624
3.022GlyPhe: 3.022 ± 0.536
5.885GlyGly: 5.885 ± 0.777
1.591GlyHis: 1.591 ± 0.376
5.01GlyIle: 5.01 ± 0.856
3.579GlyLys: 3.579 ± 0.503
6.044GlyLeu: 6.044 ± 0.711
2.147GlyMet: 2.147 ± 0.505
3.022GlyAsn: 3.022 ± 0.5
3.817GlyPro: 3.817 ± 0.771
4.295GlyGln: 4.295 ± 0.758
4.613GlyArg: 4.613 ± 0.557
4.454GlySer: 4.454 ± 0.632
5.726GlyThr: 5.726 ± 0.665
6.442GlyVal: 6.442 ± 0.802
2.068GlyTrp: 2.068 ± 0.378
3.499GlyTyr: 3.499 ± 0.563
0.0GlyXaa: 0.0 ± 0.0
His
2.147HisAla: 2.147 ± 0.384
0.159HisCys: 0.159 ± 0.126
1.75HisAsp: 1.75 ± 0.316
1.113HisGlu: 1.113 ± 0.337
1.193HisPhe: 1.193 ± 0.325
1.909HisGly: 1.909 ± 0.346
0.716HisHis: 0.716 ± 0.297
0.795HisIle: 0.795 ± 0.251
0.398HisLys: 0.398 ± 0.175
1.67HisLeu: 1.67 ± 0.382
0.636HisMet: 0.636 ± 0.199
0.636HisAsn: 0.636 ± 0.216
0.716HisPro: 0.716 ± 0.257
0.477HisGln: 0.477 ± 0.194
0.954HisArg: 0.954 ± 0.312
0.636HisSer: 0.636 ± 0.214
1.272HisThr: 1.272 ± 0.308
1.591HisVal: 1.591 ± 0.345
0.477HisTrp: 0.477 ± 0.27
0.398HisTyr: 0.398 ± 0.166
0.0HisXaa: 0.0 ± 0.0
Ile
6.203IleAla: 6.203 ± 1.071
0.159IleCys: 0.159 ± 0.116
2.704IleAsp: 2.704 ± 0.464
3.261IleGlu: 3.261 ± 0.49
0.716IlePhe: 0.716 ± 0.205
3.102IleGly: 3.102 ± 0.556
1.193IleHis: 1.193 ± 0.319
1.75IleIle: 1.75 ± 0.353
2.147IleLys: 2.147 ± 0.395
3.658IleLeu: 3.658 ± 0.501
0.795IleMet: 0.795 ± 0.244
2.227IleAsn: 2.227 ± 0.459
2.068IlePro: 2.068 ± 0.407
1.591IleGln: 1.591 ± 0.345
3.499IleArg: 3.499 ± 0.451
2.306IleSer: 2.306 ± 0.408
3.42IleThr: 3.42 ± 0.499
3.738IleVal: 3.738 ± 0.777
0.477IleTrp: 0.477 ± 0.195
1.432IleTyr: 1.432 ± 0.302
0.0IleXaa: 0.0 ± 0.0
Lys
6.362LysAla: 6.362 ± 0.749
0.557LysCys: 0.557 ± 0.214
1.75LysAsp: 1.75 ± 0.361
2.386LysGlu: 2.386 ± 0.447
0.875LysPhe: 0.875 ± 0.251
3.499LysGly: 3.499 ± 0.478
0.318LysHis: 0.318 ± 0.153
1.272LysIle: 1.272 ± 0.342
2.943LysLys: 2.943 ± 0.543
3.261LysLeu: 3.261 ± 0.557
1.034LysMet: 1.034 ± 0.281
1.352LysAsn: 1.352 ± 0.308
2.943LysPro: 2.943 ± 0.565
1.67LysGln: 1.67 ± 0.351
3.181LysArg: 3.181 ± 0.46
2.386LysSer: 2.386 ± 0.552
2.545LysThr: 2.545 ± 0.501
3.102LysVal: 3.102 ± 0.443
0.318LysTrp: 0.318 ± 0.171
0.716LysTyr: 0.716 ± 0.196
0.0LysXaa: 0.0 ± 0.0
Leu
9.782LeuAla: 9.782 ± 0.872
0.318LeuCys: 0.318 ± 0.155
5.726LeuAsp: 5.726 ± 0.696
6.044LeuGlu: 6.044 ± 0.741
2.863LeuPhe: 2.863 ± 0.35
6.442LeuGly: 6.442 ± 0.521
1.909LeuHis: 1.909 ± 0.477
4.295LeuIle: 4.295 ± 0.561
3.499LeuLys: 3.499 ± 0.491
6.203LeuLeu: 6.203 ± 0.624
1.75LeuMet: 1.75 ± 0.305
3.022LeuAsn: 3.022 ± 0.622
5.09LeuPro: 5.09 ± 0.631
2.863LeuGln: 2.863 ± 0.449
5.726LeuArg: 5.726 ± 0.668
4.613LeuSer: 4.613 ± 0.475
5.806LeuThr: 5.806 ± 0.592
6.76LeuVal: 6.76 ± 0.685
1.352LeuTrp: 1.352 ± 0.365
1.432LeuTyr: 1.432 ± 0.378
0.0LeuXaa: 0.0 ± 0.0
Met
3.42MetAla: 3.42 ± 0.453
0.0MetCys: 0.0 ± 0.0
1.511MetAsp: 1.511 ± 0.344
0.875MetGlu: 0.875 ± 0.275
0.08MetPhe: 0.08 ± 0.085
1.272MetGly: 1.272 ± 0.304
0.08MetHis: 0.08 ± 0.081
0.477MetIle: 0.477 ± 0.159
0.795MetLys: 0.795 ± 0.218
1.113MetLeu: 1.113 ± 0.321
0.239MetMet: 0.239 ± 0.13
1.511MetAsn: 1.511 ± 0.351
1.193MetPro: 1.193 ± 0.272
0.875MetGln: 0.875 ± 0.2
1.272MetArg: 1.272 ± 0.33
1.272MetSer: 1.272 ± 0.39
3.102MetThr: 3.102 ± 0.414
1.193MetVal: 1.193 ± 0.286
0.318MetTrp: 0.318 ± 0.168
0.398MetTyr: 0.398 ± 0.213
0.0MetXaa: 0.0 ± 0.0
Asn
3.42AsnAla: 3.42 ± 0.542
0.318AsnCys: 0.318 ± 0.151
1.272AsnAsp: 1.272 ± 0.251
1.193AsnGlu: 1.193 ± 0.299
1.034AsnPhe: 1.034 ± 0.256
4.692AsnGly: 4.692 ± 0.988
0.875AsnHis: 0.875 ± 0.274
1.113AsnIle: 1.113 ± 0.243
2.068AsnLys: 2.068 ± 0.458
2.943AsnLeu: 2.943 ± 0.479
0.716AsnMet: 0.716 ± 0.224
1.591AsnAsn: 1.591 ± 0.346
2.704AsnPro: 2.704 ± 0.355
1.113AsnGln: 1.113 ± 0.246
2.465AsnArg: 2.465 ± 0.527
2.863AsnSer: 2.863 ± 0.557
1.829AsnThr: 1.829 ± 0.508
1.988AsnVal: 1.988 ± 0.378
0.716AsnTrp: 0.716 ± 0.206
0.954AsnTyr: 0.954 ± 0.233
0.0AsnXaa: 0.0 ± 0.0
Pro
7.476ProAla: 7.476 ± 0.904
0.557ProCys: 0.557 ± 0.197
3.658ProAsp: 3.658 ± 0.596
3.976ProGlu: 3.976 ± 0.65
2.227ProPhe: 2.227 ± 0.482
3.976ProGly: 3.976 ± 0.571
1.193ProHis: 1.193 ± 0.286
3.181ProIle: 3.181 ± 0.421
2.863ProLys: 2.863 ± 0.565
3.34ProLeu: 3.34 ± 0.515
1.034ProMet: 1.034 ± 0.245
1.829ProAsn: 1.829 ± 0.409
1.75ProPro: 1.75 ± 0.341
1.113ProGln: 1.113 ± 0.294
2.227ProArg: 2.227 ± 0.398
2.545ProSer: 2.545 ± 0.548
3.261ProThr: 3.261 ± 0.491
2.863ProVal: 2.863 ± 0.527
1.113ProTrp: 1.113 ± 0.227
0.954ProTyr: 0.954 ± 0.287
0.0ProXaa: 0.0 ± 0.0
Gln
5.647GlnAla: 5.647 ± 0.661
0.318GlnCys: 0.318 ± 0.209
1.909GlnAsp: 1.909 ± 0.371
1.829GlnGlu: 1.829 ± 0.321
1.113GlnPhe: 1.113 ± 0.332
2.147GlnGly: 2.147 ± 0.539
0.636GlnHis: 0.636 ± 0.199
1.352GlnIle: 1.352 ± 0.264
0.875GlnLys: 0.875 ± 0.267
3.499GlnLeu: 3.499 ± 0.579
0.636GlnMet: 0.636 ± 0.206
1.67GlnAsn: 1.67 ± 0.399
1.272GlnPro: 1.272 ± 0.284
0.954GlnGln: 0.954 ± 0.308
2.227GlnArg: 2.227 ± 0.353
1.75GlnSer: 1.75 ± 0.367
1.591GlnThr: 1.591 ± 0.339
3.261GlnVal: 3.261 ± 0.565
0.716GlnTrp: 0.716 ± 0.214
1.193GlnTyr: 1.193 ± 0.26
0.0GlnXaa: 0.0 ± 0.0
Arg
6.362ArgAla: 6.362 ± 0.674
0.239ArgCys: 0.239 ± 0.167
3.42ArgAsp: 3.42 ± 0.487
3.817ArgGlu: 3.817 ± 0.611
2.068ArgPhe: 2.068 ± 0.414
4.772ArgGly: 4.772 ± 0.608
1.591ArgHis: 1.591 ± 0.357
3.181ArgIle: 3.181 ± 0.431
2.306ArgLys: 2.306 ± 0.316
5.885ArgLeu: 5.885 ± 0.778
1.034ArgMet: 1.034 ± 0.269
2.068ArgAsn: 2.068 ± 0.382
2.306ArgPro: 2.306 ± 0.402
2.068ArgGln: 2.068 ± 0.337
5.249ArgArg: 5.249 ± 0.738
4.215ArgSer: 4.215 ± 0.637
3.738ArgThr: 3.738 ± 0.411
4.613ArgVal: 4.613 ± 0.471
1.352ArgTrp: 1.352 ± 0.301
1.113ArgTyr: 1.113 ± 0.24
0.0ArgXaa: 0.0 ± 0.0
Ser
5.249SerAla: 5.249 ± 0.609
0.0SerCys: 0.0 ± 0.0
3.738SerAsp: 3.738 ± 0.521
1.988SerGlu: 1.988 ± 0.386
1.909SerPhe: 1.909 ± 0.368
5.726SerGly: 5.726 ± 0.759
0.716SerHis: 0.716 ± 0.288
2.306SerIle: 2.306 ± 0.365
2.624SerLys: 2.624 ± 0.379
5.328SerLeu: 5.328 ± 0.651
1.432SerMet: 1.432 ± 0.357
1.909SerAsn: 1.909 ± 0.499
2.863SerPro: 2.863 ± 0.413
1.909SerGln: 1.909 ± 0.413
3.817SerArg: 3.817 ± 0.455
3.022SerSer: 3.022 ± 0.521
3.976SerThr: 3.976 ± 0.473
2.704SerVal: 2.704 ± 0.479
1.272SerTrp: 1.272 ± 0.27
0.716SerTyr: 0.716 ± 0.24
0.0SerXaa: 0.0 ± 0.0
Thr
6.999ThrAla: 6.999 ± 0.871
0.239ThrCys: 0.239 ± 0.135
4.851ThrAsp: 4.851 ± 0.521
4.136ThrGlu: 4.136 ± 0.451
2.306ThrPhe: 2.306 ± 0.357
6.203ThrGly: 6.203 ± 0.697
1.432ThrHis: 1.432 ± 0.317
4.295ThrIle: 4.295 ± 0.509
3.738ThrLys: 3.738 ± 0.56
5.01ThrLeu: 5.01 ± 0.712
1.352ThrMet: 1.352 ± 0.27
1.829ThrAsn: 1.829 ± 0.341
4.056ThrPro: 4.056 ± 0.567
1.272ThrGln: 1.272 ± 0.269
3.42ThrArg: 3.42 ± 0.526
3.181ThrSer: 3.181 ± 0.389
4.851ThrThr: 4.851 ± 0.712
4.533ThrVal: 4.533 ± 0.773
1.352ThrTrp: 1.352 ± 0.277
1.272ThrTyr: 1.272 ± 0.293
0.0ThrXaa: 0.0 ± 0.0
Val
8.032ValAla: 8.032 ± 0.708
0.398ValCys: 0.398 ± 0.186
4.931ValAsp: 4.931 ± 0.532
4.454ValGlu: 4.454 ± 0.55
2.465ValPhe: 2.465 ± 0.532
5.647ValGly: 5.647 ± 0.678
1.432ValHis: 1.432 ± 0.339
3.181ValIle: 3.181 ± 0.557
2.386ValLys: 2.386 ± 0.484
6.362ValLeu: 6.362 ± 0.667
1.352ValMet: 1.352 ± 0.3
2.147ValAsn: 2.147 ± 0.397
3.897ValPro: 3.897 ± 0.508
2.147ValGln: 2.147 ± 0.388
4.454ValArg: 4.454 ± 0.54
3.261ValSer: 3.261 ± 0.487
4.613ValThr: 4.613 ± 0.652
5.647ValVal: 5.647 ± 0.676
1.511ValTrp: 1.511 ± 0.365
2.863ValTyr: 2.863 ± 0.545
0.0ValXaa: 0.0 ± 0.0
Trp
2.306TrpAla: 2.306 ± 0.414
0.08TrpCys: 0.08 ± 0.07
0.636TrpAsp: 0.636 ± 0.221
0.954TrpGlu: 0.954 ± 0.247
0.954TrpPhe: 0.954 ± 0.263
1.352TrpGly: 1.352 ± 0.26
0.159TrpHis: 0.159 ± 0.116
0.875TrpIle: 0.875 ± 0.265
0.716TrpLys: 0.716 ± 0.23
1.988TrpLeu: 1.988 ± 0.375
0.318TrpMet: 0.318 ± 0.144
1.272TrpAsn: 1.272 ± 0.29
0.398TrpPro: 0.398 ± 0.175
0.954TrpGln: 0.954 ± 0.295
0.795TrpArg: 0.795 ± 0.214
1.432TrpSer: 1.432 ± 0.353
1.511TrpThr: 1.511 ± 0.318
1.591TrpVal: 1.591 ± 0.287
0.318TrpTrp: 0.318 ± 0.14
0.477TrpTyr: 0.477 ± 0.173
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.943TyrAla: 2.943 ± 0.563
0.08TyrCys: 0.08 ± 0.078
2.227TyrAsp: 2.227 ± 0.378
1.591TyrGlu: 1.591 ± 0.368
0.398TyrPhe: 0.398 ± 0.153
2.784TyrGly: 2.784 ± 0.546
0.239TyrHis: 0.239 ± 0.133
1.432TyrIle: 1.432 ± 0.349
0.636TyrLys: 0.636 ± 0.203
1.829TyrLeu: 1.829 ± 0.363
0.716TyrMet: 0.716 ± 0.199
0.954TyrAsn: 0.954 ± 0.316
1.75TyrPro: 1.75 ± 0.303
1.113TyrGln: 1.113 ± 0.308
2.068TyrArg: 2.068 ± 0.382
1.193TyrSer: 1.193 ± 0.366
1.511TyrThr: 1.511 ± 0.324
0.795TyrVal: 0.795 ± 0.284
0.557TyrTrp: 0.557 ± 0.18
0.795TyrTyr: 0.795 ± 0.286
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 68 proteins (12575 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski