Streptococcus phage Javan331

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; unclassified Siphoviridae

Average proteome isoelectric point is 6.18

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 56 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A4D6A6Y6|A0A4D6A6Y6_9CAUD Uncharacterized protein OS=Streptococcus phage Javan331 OX=2548111 GN=Javan331_0015 PE=4 SV=1
MM1 pKa = 8.1AYY3 pKa = 10.01QVIRR7 pKa = 11.84PFKK10 pKa = 10.32DD11 pKa = 3.11LRR13 pKa = 11.84DD14 pKa = 3.88PQQYY18 pKa = 9.81EE19 pKa = 4.0YY20 pKa = 10.82QIGDD24 pKa = 3.73IYY26 pKa = 10.71PRR28 pKa = 11.84TGYY31 pKa = 10.9KK32 pKa = 8.42STKK35 pKa = 9.28TFIQEE40 pKa = 4.32LLDD43 pKa = 4.01GSNSAGSIFLTKK55 pKa = 9.67IDD57 pKa = 4.7DD58 pKa = 3.68VDD60 pKa = 3.33ISEE63 pKa = 5.19GEE65 pKa = 4.15TEE67 pKa = 3.75PRR69 pKa = 11.84EE70 pKa = 4.07PEE72 pKa = 4.15EE73 pKa = 4.23EE74 pKa = 4.14DD75 pKa = 3.55EE76 pKa = 4.34EE77 pKa = 4.47

Molecular weight: 8.94 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A4D6ABR3|A0A4D6ABR3_9CAUD Cro/CI family transcriptional regulator OS=Streptococcus phage Javan331 OX=2548111 GN=Javan331_0052 PE=4 SV=1
MM1 pKa = 7.32YY2 pKa = 10.09KK3 pKa = 10.46FSNAKK8 pKa = 9.12SVKK11 pKa = 9.05ISLAIRR17 pKa = 11.84FLSFLRR23 pKa = 11.84NQVVAVNCSVVNPDD37 pKa = 3.27VFSTHH42 pKa = 6.59AFTKK46 pKa = 9.96AWIVNSRR53 pKa = 11.84VAGLMLLL60 pKa = 4.06

Molecular weight: 6.7 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

56

0

56

10524

37

938

187.9

21.3

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.67 ± 0.412

0.627 ± 0.106

6.005 ± 0.307

8.219 ± 0.355

4.323 ± 0.298

6.499 ± 0.416

1.378 ± 0.167

6.918 ± 0.278

8.115 ± 0.357

8.381 ± 0.346

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.385 ± 0.151

5.948 ± 0.311

2.946 ± 0.192

3.839 ± 0.263

4.285 ± 0.223

5.882 ± 0.299

6.167 ± 0.355

6.29 ± 0.224

1.378 ± 0.182

3.744 ± 0.363
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski