Amino acid dipepetide frequency for Streptococcus phage Javan331

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
4.371AlaAla: 4.371 ± 1.182
0.475AlaCys: 0.475 ± 0.187
4.086AlaAsp: 4.086 ± 0.725
5.512AlaGlu: 5.512 ± 0.689
2.186AlaPhe: 2.186 ± 0.36
3.896AlaGly: 3.896 ± 0.604
0.475AlaHis: 0.475 ± 0.18
5.987AlaIle: 5.987 ± 0.999
4.561AlaLys: 4.561 ± 0.845
6.937AlaLeu: 6.937 ± 0.955
1.425AlaMet: 1.425 ± 0.327
3.421AlaAsn: 3.421 ± 0.673
1.711AlaPro: 1.711 ± 0.444
3.136AlaGln: 3.136 ± 0.695
3.421AlaArg: 3.421 ± 0.445
2.946AlaSer: 2.946 ± 0.636
3.706AlaThr: 3.706 ± 0.684
4.656AlaVal: 4.656 ± 0.698
0.95AlaTrp: 0.95 ± 0.318
2.851AlaTyr: 2.851 ± 0.633
0.0AlaXaa: 0.0 ± 0.0
Cys
0.095CysAla: 0.095 ± 0.092
0.0CysCys: 0.0 ± 0.0
0.0CysAsp: 0.0 ± 0.0
0.855CysGlu: 0.855 ± 0.243
0.095CysPhe: 0.095 ± 0.086
0.095CysGly: 0.095 ± 0.101
0.19CysHis: 0.19 ± 0.135
0.38CysIle: 0.38 ± 0.229
0.57CysLys: 0.57 ± 0.242
0.76CysLeu: 0.76 ± 0.231
0.0CysMet: 0.0 ± 0.0
0.665CysAsn: 0.665 ± 0.282
0.38CysPro: 0.38 ± 0.221
0.285CysGln: 0.285 ± 0.15
0.285CysArg: 0.285 ± 0.165
0.475CysSer: 0.475 ± 0.216
0.285CysThr: 0.285 ± 0.149
0.19CysVal: 0.19 ± 0.116
0.285CysTrp: 0.285 ± 0.15
0.38CysTyr: 0.38 ± 0.196
0.0CysXaa: 0.0 ± 0.0
Asp
3.231AspAla: 3.231 ± 0.54
0.095AspCys: 0.095 ± 0.101
4.086AspAsp: 4.086 ± 0.744
4.751AspGlu: 4.751 ± 0.991
2.281AspPhe: 2.281 ± 0.509
5.227AspGly: 5.227 ± 0.92
0.57AspHis: 0.57 ± 0.227
6.272AspIle: 6.272 ± 0.682
4.181AspLys: 4.181 ± 0.66
4.942AspLeu: 4.942 ± 0.578
1.33AspMet: 1.33 ± 0.33
2.946AspAsn: 2.946 ± 0.493
1.616AspPro: 1.616 ± 0.411
1.14AspGln: 1.14 ± 0.302
2.376AspArg: 2.376 ± 0.472
3.706AspSer: 3.706 ± 0.526
3.136AspThr: 3.136 ± 0.625
3.611AspVal: 3.611 ± 0.358
1.045AspTrp: 1.045 ± 0.3
3.516AspTyr: 3.516 ± 0.663
0.0AspXaa: 0.0 ± 0.0
Glu
4.656GluAla: 4.656 ± 0.629
0.285GluCys: 0.285 ± 0.163
4.086GluAsp: 4.086 ± 0.684
7.602GluGlu: 7.602 ± 1.035
3.611GluPhe: 3.611 ± 0.688
3.611GluGly: 3.611 ± 0.553
0.95GluHis: 0.95 ± 0.268
5.132GluIle: 5.132 ± 0.59
7.507GluLys: 7.507 ± 0.885
9.883GluLeu: 9.883 ± 0.966
3.041GluMet: 3.041 ± 0.42
4.276GluAsn: 4.276 ± 0.669
1.711GluPro: 1.711 ± 0.549
3.611GluGln: 3.611 ± 0.706
4.276GluArg: 4.276 ± 0.634
4.751GluSer: 4.751 ± 0.669
3.991GluThr: 3.991 ± 0.571
5.227GluVal: 5.227 ± 0.69
1.14GluTrp: 1.14 ± 0.288
2.851GluTyr: 2.851 ± 0.603
0.0GluXaa: 0.0 ± 0.0
Phe
2.376PheAla: 2.376 ± 0.484
0.38PheCys: 0.38 ± 0.207
2.946PheAsp: 2.946 ± 0.528
3.041PheGlu: 3.041 ± 0.621
2.376PhePhe: 2.376 ± 0.552
3.706PheGly: 3.706 ± 0.724
0.475PheHis: 0.475 ± 0.233
3.706PheIle: 3.706 ± 0.53
3.611PheLys: 3.611 ± 0.489
3.516PheLeu: 3.516 ± 0.658
0.95PheMet: 0.95 ± 0.287
2.566PheAsn: 2.566 ± 0.472
1.52PhePro: 1.52 ± 0.365
0.95PheGln: 0.95 ± 0.289
1.616PheArg: 1.616 ± 0.309
2.376PheSer: 2.376 ± 0.382
2.471PheThr: 2.471 ± 0.411
2.471PheVal: 2.471 ± 0.496
0.665PheTrp: 0.665 ± 0.237
1.52PheTyr: 1.52 ± 0.345
0.0PheXaa: 0.0 ± 0.0
Gly
4.847GlyAla: 4.847 ± 1.023
0.095GlyCys: 0.095 ± 0.097
5.702GlyAsp: 5.702 ± 0.579
4.371GlyGlu: 4.371 ± 0.656
2.946GlyPhe: 2.946 ± 0.46
4.561GlyGly: 4.561 ± 0.862
0.76GlyHis: 0.76 ± 0.301
4.086GlyIle: 4.086 ± 1.053
4.847GlyLys: 4.847 ± 0.521
4.751GlyLeu: 4.751 ± 0.823
1.235GlyMet: 1.235 ± 0.367
5.322GlyAsn: 5.322 ± 0.992
1.235GlyPro: 1.235 ± 0.721
2.091GlyGln: 2.091 ± 0.42
2.281GlyArg: 2.281 ± 0.424
3.136GlySer: 3.136 ± 0.446
4.371GlyThr: 4.371 ± 0.726
3.991GlyVal: 3.991 ± 0.538
1.33GlyTrp: 1.33 ± 0.568
2.946GlyTyr: 2.946 ± 0.604
0.0GlyXaa: 0.0 ± 0.0
His
0.665HisAla: 0.665 ± 0.229
0.095HisCys: 0.095 ± 0.085
0.57HisAsp: 0.57 ± 0.251
0.76HisGlu: 0.76 ± 0.267
1.52HisPhe: 1.52 ± 0.3
0.38HisGly: 0.38 ± 0.195
0.475HisHis: 0.475 ± 0.204
1.52HisIle: 1.52 ± 0.378
0.665HisLys: 0.665 ± 0.264
1.425HisLeu: 1.425 ± 0.344
0.38HisMet: 0.38 ± 0.171
0.76HisAsn: 0.76 ± 0.28
0.475HisPro: 0.475 ± 0.207
0.57HisGln: 0.57 ± 0.242
0.475HisArg: 0.475 ± 0.198
0.665HisSer: 0.665 ± 0.215
0.95HisThr: 0.95 ± 0.292
0.855HisVal: 0.855 ± 0.25
0.19HisTrp: 0.19 ± 0.139
0.38HisTyr: 0.38 ± 0.164
0.0HisXaa: 0.0 ± 0.0
Ile
5.607IleAla: 5.607 ± 0.668
0.475IleCys: 0.475 ± 0.244
4.181IleAsp: 4.181 ± 0.596
5.702IleGlu: 5.702 ± 0.818
3.326IlePhe: 3.326 ± 0.495
4.371IleGly: 4.371 ± 0.846
0.855IleHis: 0.855 ± 0.292
4.276IleIle: 4.276 ± 0.655
6.937IleLys: 6.937 ± 0.868
4.276IleLeu: 4.276 ± 0.69
1.33IleMet: 1.33 ± 0.31
2.946IleAsn: 2.946 ± 0.52
2.471IlePro: 2.471 ± 0.465
3.231IleGln: 3.231 ± 0.643
2.756IleArg: 2.756 ± 0.383
5.227IleSer: 5.227 ± 0.82
5.322IleThr: 5.322 ± 0.734
3.421IleVal: 3.421 ± 0.596
0.57IleTrp: 0.57 ± 0.34
1.901IleTyr: 1.901 ± 0.506
0.0IleXaa: 0.0 ± 0.0
Lys
5.322LysAla: 5.322 ± 0.75
0.285LysCys: 0.285 ± 0.133
5.607LysAsp: 5.607 ± 0.77
7.032LysGlu: 7.032 ± 0.782
2.661LysPhe: 2.661 ± 0.451
4.371LysGly: 4.371 ± 0.73
1.14LysHis: 1.14 ± 0.285
5.227LysIle: 5.227 ± 0.705
7.222LysLys: 7.222 ± 0.831
5.892LysLeu: 5.892 ± 0.745
2.376LysMet: 2.376 ± 0.398
4.561LysAsn: 4.561 ± 0.792
2.566LysPro: 2.566 ± 0.412
3.421LysGln: 3.421 ± 0.539
4.656LysArg: 4.656 ± 0.804
4.942LysSer: 4.942 ± 0.709
4.656LysThr: 4.656 ± 0.701
5.417LysVal: 5.417 ± 0.689
1.235LysTrp: 1.235 ± 0.308
2.566LysTyr: 2.566 ± 0.529
0.0LysXaa: 0.0 ± 0.0
Leu
5.417LeuAla: 5.417 ± 0.768
0.475LeuCys: 0.475 ± 0.225
5.607LeuAsp: 5.607 ± 0.652
9.123LeuGlu: 9.123 ± 1.033
3.706LeuPhe: 3.706 ± 0.845
5.702LeuGly: 5.702 ± 0.75
1.711LeuHis: 1.711 ± 0.351
4.276LeuIle: 4.276 ± 0.633
8.743LeuLys: 8.743 ± 1.02
5.702LeuLeu: 5.702 ± 0.792
2.376LeuMet: 2.376 ± 0.521
3.706LeuAsn: 3.706 ± 0.554
2.566LeuPro: 2.566 ± 0.5
2.661LeuGln: 2.661 ± 0.739
3.706LeuArg: 3.706 ± 0.705
5.037LeuSer: 5.037 ± 0.928
5.987LeuThr: 5.987 ± 0.656
4.656LeuVal: 4.656 ± 0.609
0.665LeuTrp: 0.665 ± 0.236
1.996LeuTyr: 1.996 ± 0.381
0.0LeuXaa: 0.0 ± 0.0
Met
1.711MetAla: 1.711 ± 0.362
0.0MetCys: 0.0 ± 0.0
1.425MetAsp: 1.425 ± 0.35
2.091MetGlu: 2.091 ± 0.406
0.95MetPhe: 0.95 ± 0.368
1.425MetGly: 1.425 ± 0.348
0.095MetHis: 0.095 ± 0.083
1.425MetIle: 1.425 ± 0.298
1.711MetLys: 1.711 ± 0.431
1.711MetLeu: 1.711 ± 0.334
0.855MetMet: 0.855 ± 0.262
1.806MetAsn: 1.806 ± 0.377
0.57MetPro: 0.57 ± 0.228
0.57MetGln: 0.57 ± 0.222
1.711MetArg: 1.711 ± 0.428
1.33MetSer: 1.33 ± 0.267
2.091MetThr: 2.091 ± 0.488
1.52MetVal: 1.52 ± 0.35
0.19MetTrp: 0.19 ± 0.121
0.665MetTyr: 0.665 ± 0.24
0.0MetXaa: 0.0 ± 0.0
Asn
3.896AsnAla: 3.896 ± 0.734
0.38AsnCys: 0.38 ± 0.199
3.326AsnAsp: 3.326 ± 0.591
4.276AsnGlu: 4.276 ± 0.584
2.186AsnPhe: 2.186 ± 0.33
5.417AsnGly: 5.417 ± 0.811
0.855AsnHis: 0.855 ± 0.342
2.756AsnIle: 2.756 ± 0.541
5.322AsnLys: 5.322 ± 0.7
4.847AsnLeu: 4.847 ± 0.704
1.14AsnMet: 1.14 ± 0.392
3.801AsnAsn: 3.801 ± 0.554
2.851AsnPro: 2.851 ± 0.426
3.326AsnGln: 3.326 ± 0.533
2.566AsnArg: 2.566 ± 0.374
3.421AsnSer: 3.421 ± 0.462
3.421AsnThr: 3.421 ± 0.463
3.326AsnVal: 3.326 ± 0.657
0.76AsnTrp: 0.76 ± 0.265
1.616AsnTyr: 1.616 ± 0.394
0.0AsnXaa: 0.0 ± 0.0
Pro
2.091ProAla: 2.091 ± 0.457
0.57ProCys: 0.57 ± 0.251
1.711ProAsp: 1.711 ± 0.522
2.471ProGlu: 2.471 ± 0.441
1.235ProPhe: 1.235 ± 0.315
1.235ProGly: 1.235 ± 0.394
0.57ProHis: 0.57 ± 0.212
1.806ProIle: 1.806 ± 0.39
2.186ProLys: 2.186 ± 0.449
2.946ProLeu: 2.946 ± 0.392
0.38ProMet: 0.38 ± 0.19
1.14ProAsn: 1.14 ± 0.401
0.285ProPro: 0.285 ± 0.179
1.52ProGln: 1.52 ± 0.383
0.855ProArg: 0.855 ± 0.3
2.186ProSer: 2.186 ± 0.48
2.756ProThr: 2.756 ± 0.53
2.186ProVal: 2.186 ± 0.469
0.38ProTrp: 0.38 ± 0.189
0.95ProTyr: 0.95 ± 0.338
0.0ProXaa: 0.0 ± 0.0
Gln
4.371GlnAla: 4.371 ± 0.86
0.19GlnCys: 0.19 ± 0.119
1.14GlnAsp: 1.14 ± 0.279
2.851GlnGlu: 2.851 ± 0.443
1.425GlnPhe: 1.425 ± 0.359
2.566GlnGly: 2.566 ± 0.534
0.475GlnHis: 0.475 ± 0.193
2.471GlnIle: 2.471 ± 0.51
2.661GlnLys: 2.661 ± 0.458
3.326GlnLeu: 3.326 ± 0.556
1.235GlnMet: 1.235 ± 0.335
2.376GlnAsn: 2.376 ± 0.456
0.855GlnPro: 0.855 ± 0.243
1.711GlnGln: 1.711 ± 0.465
1.14GlnArg: 1.14 ± 0.34
2.471GlnSer: 2.471 ± 0.421
3.611GlnThr: 3.611 ± 0.509
2.186GlnVal: 2.186 ± 0.369
0.285GlnTrp: 0.285 ± 0.157
1.045GlnTyr: 1.045 ± 0.281
0.0GlnXaa: 0.0 ± 0.0
Arg
3.611ArgAla: 3.611 ± 0.532
0.38ArgCys: 0.38 ± 0.204
2.186ArgAsp: 2.186 ± 0.496
3.326ArgGlu: 3.326 ± 0.612
1.52ArgPhe: 1.52 ± 0.434
2.091ArgGly: 2.091 ± 0.402
0.475ArgHis: 0.475 ± 0.19
3.611ArgIle: 3.611 ± 0.552
3.516ArgLys: 3.516 ± 0.681
3.611ArgLeu: 3.611 ± 0.592
0.57ArgMet: 0.57 ± 0.211
3.516ArgAsn: 3.516 ± 0.453
1.52ArgPro: 1.52 ± 0.425
1.14ArgGln: 1.14 ± 0.286
1.33ArgArg: 1.33 ± 0.36
2.376ArgSer: 2.376 ± 0.509
1.901ArgThr: 1.901 ± 0.36
2.376ArgVal: 2.376 ± 0.551
0.665ArgTrp: 0.665 ± 0.224
3.136ArgTyr: 3.136 ± 0.554
0.0ArgXaa: 0.0 ± 0.0
Ser
4.371SerAla: 4.371 ± 0.61
0.475SerCys: 0.475 ± 0.194
2.851SerAsp: 2.851 ± 0.581
5.037SerGlu: 5.037 ± 0.703
3.326SerPhe: 3.326 ± 0.545
4.181SerGly: 4.181 ± 0.665
0.95SerHis: 0.95 ± 0.224
4.181SerIle: 4.181 ± 0.777
2.946SerLys: 2.946 ± 0.57
4.847SerLeu: 4.847 ± 0.585
1.52SerMet: 1.52 ± 0.375
3.991SerAsn: 3.991 ± 0.596
1.996SerPro: 1.996 ± 0.419
1.996SerGln: 1.996 ± 0.436
1.806SerArg: 1.806 ± 0.381
3.041SerSer: 3.041 ± 0.622
3.516SerThr: 3.516 ± 0.635
4.466SerVal: 4.466 ± 0.588
0.76SerTrp: 0.76 ± 0.266
2.566SerTyr: 2.566 ± 0.549
0.0SerXaa: 0.0 ± 0.0
Thr
3.896ThrAla: 3.896 ± 0.781
0.285ThrCys: 0.285 ± 0.172
4.181ThrAsp: 4.181 ± 0.896
4.276ThrGlu: 4.276 ± 0.716
2.471ThrPhe: 2.471 ± 0.529
4.466ThrGly: 4.466 ± 0.703
1.045ThrHis: 1.045 ± 0.265
4.751ThrIle: 4.751 ± 0.606
5.132ThrLys: 5.132 ± 0.81
5.322ThrLeu: 5.322 ± 0.681
1.425ThrMet: 1.425 ± 0.307
3.896ThrAsn: 3.896 ± 0.621
1.33ThrPro: 1.33 ± 0.369
2.661ThrGln: 2.661 ± 0.563
3.136ThrArg: 3.136 ± 0.399
3.136ThrSer: 3.136 ± 0.57
3.231ThrThr: 3.231 ± 0.442
4.847ThrVal: 4.847 ± 0.876
0.76ThrTrp: 0.76 ± 0.282
1.425ThrTyr: 1.425 ± 0.507
0.0ThrXaa: 0.0 ± 0.0
Val
3.706ValAla: 3.706 ± 0.649
0.475ValCys: 0.475 ± 0.24
3.896ValAsp: 3.896 ± 0.536
4.942ValGlu: 4.942 ± 0.715
2.661ValPhe: 2.661 ± 0.479
4.466ValGly: 4.466 ± 0.654
0.855ValHis: 0.855 ± 0.307
3.991ValIle: 3.991 ± 0.754
4.847ValLys: 4.847 ± 0.736
4.561ValLeu: 4.561 ± 0.447
1.52ValMet: 1.52 ± 0.335
4.086ValAsn: 4.086 ± 0.625
1.711ValPro: 1.711 ± 0.408
1.52ValGln: 1.52 ± 0.341
2.091ValArg: 2.091 ± 0.381
4.847ValSer: 4.847 ± 0.607
5.132ValThr: 5.132 ± 0.702
5.037ValVal: 5.037 ± 0.666
0.665ValTrp: 0.665 ± 0.348
1.901ValTyr: 1.901 ± 0.443
0.0ValXaa: 0.0 ± 0.0
Trp
0.665TrpAla: 0.665 ± 0.267
0.475TrpCys: 0.475 ± 0.239
0.19TrpAsp: 0.19 ± 0.135
0.95TrpGlu: 0.95 ± 0.383
0.665TrpPhe: 0.665 ± 0.264
0.665TrpGly: 0.665 ± 0.248
0.285TrpHis: 0.285 ± 0.14
1.045TrpIle: 1.045 ± 0.312
1.235TrpLys: 1.235 ± 0.38
1.33TrpLeu: 1.33 ± 0.352
0.095TrpMet: 0.095 ± 0.114
1.52TrpAsn: 1.52 ± 0.537
0.285TrpPro: 0.285 ± 0.158
0.665TrpGln: 0.665 ± 0.233
0.475TrpArg: 0.475 ± 0.23
1.045TrpSer: 1.045 ± 0.263
0.285TrpThr: 0.285 ± 0.157
0.665TrpVal: 0.665 ± 0.208
0.38TrpTrp: 0.38 ± 0.191
0.855TrpTyr: 0.855 ± 0.537
0.0TrpXaa: 0.0 ± 0.0
Tyr
1.806TyrAla: 1.806 ± 0.406
0.38TyrCys: 0.38 ± 0.217
2.281TyrAsp: 2.281 ± 0.399
3.231TyrGlu: 3.231 ± 0.54
2.091TyrPhe: 2.091 ± 0.539
2.566TyrGly: 2.566 ± 0.562
0.57TyrHis: 0.57 ± 0.218
2.281TyrIle: 2.281 ± 0.464
2.756TyrLys: 2.756 ± 0.536
3.231TyrLeu: 3.231 ± 0.501
0.57TyrMet: 0.57 ± 0.205
2.186TyrAsn: 2.186 ± 0.422
1.806TyrPro: 1.806 ± 0.407
2.186TyrGln: 2.186 ± 0.487
1.901TyrArg: 1.901 ± 0.364
1.711TyrSer: 1.711 ± 0.395
0.855TyrThr: 0.855 ± 0.26
1.806TyrVal: 1.806 ± 0.428
0.855TyrTrp: 0.855 ± 0.284
2.376TyrTyr: 2.376 ± 0.651
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 56 proteins (10524 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski