Botrytis virus X (isolate Botrytis cinerea/New Zealand/Howitt/2006) (BOTV-X)

Taxonomy: Viruses; Riboviria; Orthornavirae; Kitrinoviricota; Alsuviricetes; Tymovirales; Alphaflexiviridae; Botrexvirus; Botrytis virus X

Average proteome isoelectric point is 6.6

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 5 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>sp|Q6YNQ6|ORF2_BOTVX Uncharacterized ORF2 protein OS=Botrytis virus X (isolate Botrytis cinerea/New Zealand/Howitt/2006) OX=686947 GN=ORF2 PE=4 SV=1
MM1 pKa = 8.1DD2 pKa = 6.13PNLDD6 pKa = 3.52QDD8 pKa = 4.01TLPTHH13 pKa = 6.28EE14 pKa = 5.49EE15 pKa = 3.43IDD17 pKa = 3.64NDD19 pKa = 3.22VDD21 pKa = 3.96SAEE24 pKa = 4.44EE25 pKa = 4.17EE26 pKa = 4.47PPEE29 pKa = 4.71PPLLPDD35 pKa = 6.43DD36 pKa = 4.92IDD38 pKa = 6.46DD39 pKa = 5.03DD40 pKa = 4.91DD41 pKa = 4.31SHH43 pKa = 7.56GSRR46 pKa = 11.84TRR48 pKa = 11.84RR49 pKa = 11.84QVKK52 pKa = 9.46PPPEE56 pKa = 3.96LLRR59 pKa = 11.84AVGACLISGHH69 pKa = 6.0YY70 pKa = 10.5DD71 pKa = 2.82GGNYY75 pKa = 9.11FRR77 pKa = 11.84WQQSIAALYY86 pKa = 10.27AKK88 pKa = 10.08AGYY91 pKa = 10.43AGDD94 pKa = 3.63IRR96 pKa = 11.84FHH98 pKa = 5.58QAAIQEE104 pKa = 4.39YY105 pKa = 9.98ALDD108 pKa = 4.12PVLPAPRR115 pKa = 11.84VSYY118 pKa = 11.09DD119 pKa = 3.62LLVAHH124 pKa = 7.34AGLRR128 pKa = 11.84YY129 pKa = 8.98QALLNEE135 pKa = 4.4QLRR138 pKa = 11.84TGKK141 pKa = 8.24TPPADD146 pKa = 3.73EE147 pKa = 4.38ALKK150 pKa = 10.9DD151 pKa = 3.92AVRR154 pKa = 11.84KK155 pKa = 8.83AAQAAYY161 pKa = 10.36DD162 pKa = 3.92NAVKK166 pKa = 9.95TGDD169 pKa = 3.88YY170 pKa = 10.13TPLIDD175 pKa = 4.17IAFKK179 pKa = 10.78GVDD182 pKa = 3.64INKK185 pKa = 9.22HH186 pKa = 5.38ASDD189 pKa = 3.78VAQLAKK195 pKa = 10.25MSVTMDD201 pKa = 3.05GTHH204 pKa = 6.45IKK206 pKa = 8.88FTAGEE211 pKa = 4.12MPKK214 pKa = 10.87DD215 pKa = 3.53KK216 pKa = 11.04VITSNSMASPNTVMNILNLITTSANVTAVTCGIEE250 pKa = 3.86FAIACAHH257 pKa = 5.87QGSSRR262 pKa = 11.84YY263 pKa = 7.37TRR265 pKa = 11.84HH266 pKa = 6.2TGTSTGGSTFEE277 pKa = 5.42LIAAHH282 pKa = 5.85VKK284 pKa = 7.25EE285 pKa = 4.64HH286 pKa = 5.26CTIRR290 pKa = 11.84QFCSYY295 pKa = 9.46FAKK298 pKa = 10.67VVWNHH303 pKa = 6.35LLTHH307 pKa = 6.5ATPPVNWAKK316 pKa = 10.73HH317 pKa = 4.94GFTLDD322 pKa = 2.93SRR324 pKa = 11.84YY325 pKa = 10.45AAFDD329 pKa = 3.5FFDD332 pKa = 3.86AVTNAAALPPKK343 pKa = 10.12NGLIRR348 pKa = 11.84APTSEE353 pKa = 4.91EE354 pKa = 3.46IRR356 pKa = 11.84AHH358 pKa = 6.02NLNAHH363 pKa = 6.37LLINASRR370 pKa = 11.84QDD372 pKa = 3.76DD373 pKa = 4.04QVSSSAQYY381 pKa = 9.0TAAIAQAGGFKK392 pKa = 10.3RR393 pKa = 11.84PQIGWGEE400 pKa = 3.9

Molecular weight: 43.5 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>sp|Q6YNQ5|CAPSD_BOTVX Capsid protein OS=Botrytis virus X (isolate Botrytis cinerea/New Zealand/Howitt/2006) OX=686947 GN=ORF3 PE=3 SV=1
MM1 pKa = 7.16PTYY4 pKa = 11.16SSMLHH9 pKa = 5.91AKK11 pKa = 9.06MIKK14 pKa = 10.1SPAALNTPQPSPKK27 pKa = 9.52QAVSNAHH34 pKa = 6.49RR35 pKa = 11.84LDD37 pKa = 4.14GGSSYY42 pKa = 11.09VVSLSEE48 pKa = 4.32ISSCPTSTTNSLFAAATPSTLLLSALYY75 pKa = 7.47CTPPSQLRR83 pKa = 11.84MKK85 pKa = 10.51SSCTLTLFIPPLNTAASPTLPDD107 pKa = 3.93TSCHH111 pKa = 6.07GEE113 pKa = 3.9WNTTTRR119 pKa = 11.84PCLAASTALMYY130 pKa = 10.98SLL132 pKa = 5.14

Molecular weight: 13.88 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

5

0

5

2351

128

1413

470.2

52.01

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.485 ± 1.041

1.148 ± 0.41

6.125 ± 0.791

4.466 ± 0.692

4.041 ± 0.459

4.381 ± 0.539

4.339 ± 0.502

4.892 ± 0.852

4.806 ± 0.812

10.038 ± 0.795

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.829 ± 0.43

4.083 ± 0.233

8.507 ± 1.763

3.488 ± 0.572

4.424 ± 0.429

6.465 ± 1.43

8.762 ± 0.564

5.019 ± 0.451

0.808 ± 0.046

2.892 ± 0.259
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski