Pedobacter sp. BAL39

Taxonomy: cellular organisms; Bacteria; FCB group; Bacteroidetes/Chlorobi group; Bacteroidetes; Sphingobacteriia; Sphingobacteriales; Sphingobacteriaceae; Pedobacter; unclassified Pedobacter

Average proteome isoelectric point is 6.74

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 5097 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A6E9F8|A6E9F8_9SPHI Uncharacterized protein OS=Pedobacter sp. BAL39 OX=391596 GN=PBAL39_15229 PE=4 SV=1
MM1 pKa = 7.35IAINNVQPLSPDD13 pKa = 3.25SLFDD17 pKa = 4.2LLKK20 pKa = 9.91TDD22 pKa = 4.47FPAYY26 pKa = 9.74INEE29 pKa = 4.07QLGSNLAVEE38 pKa = 5.14FAHH41 pKa = 7.07VSDD44 pKa = 3.93IVNISFPEE52 pKa = 4.29VIEE55 pKa = 4.39GNAYY59 pKa = 8.88TITVGEE65 pKa = 4.63DD66 pKa = 2.93SLDD69 pKa = 3.62ITDD72 pKa = 4.02HH73 pKa = 5.0TTEE76 pKa = 3.81GTYY79 pKa = 8.59NTEE82 pKa = 3.93LLEE85 pKa = 3.85QHH87 pKa = 6.97LIEE90 pKa = 5.23FLTLKK95 pKa = 10.8AGG97 pKa = 3.51

Molecular weight: 10.7 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A6EBJ8|A6EBJ8_9SPHI Glutamyl-tRNA(Gln) amidotransferase subunit A OS=Pedobacter sp. BAL39 OX=391596 GN=gatA PE=3 SV=1
MM1 pKa = 7.88RR2 pKa = 11.84LFRR5 pKa = 11.84SKK7 pKa = 10.24KK8 pKa = 9.56QSISPSADD16 pKa = 2.87TLRR19 pKa = 11.84GKK21 pKa = 9.82LASYY25 pKa = 10.64ILRR28 pKa = 11.84QQSRR32 pKa = 11.84IAGWLNEE39 pKa = 4.23RR40 pKa = 11.84TSSYY44 pKa = 11.46SPFRR48 pKa = 11.84WMVLLILFCLGVGGICIYY66 pKa = 10.71LIIISII72 pKa = 3.96

Molecular weight: 8.24 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

5097

0

5097

1742446

20

7799

341.9

38.28

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.656 ± 0.043

0.717 ± 0.011

5.404 ± 0.023

5.75 ± 0.042

4.864 ± 0.025

6.948 ± 0.042

1.846 ± 0.018

6.939 ± 0.033

6.662 ± 0.039

9.623 ± 0.035

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.44 ± 0.02

5.438 ± 0.038

3.746 ± 0.02

3.84 ± 0.022

4.182 ± 0.023

6.621 ± 0.027

5.73 ± 0.044

6.36 ± 0.029

1.138 ± 0.013

4.094 ± 0.025
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski