Amino acid dipepetide frequency for Pedobacter sp. BAL39

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
7.24AlaAla: 7.24 ± 0.093
0.609AlaCys: 0.609 ± 0.022
4.669AlaAsp: 4.669 ± 0.052
4.777AlaGlu: 4.777 ± 0.064
3.546AlaPhe: 3.546 ± 0.046
5.891AlaGly: 5.891 ± 0.087
1.254AlaHis: 1.254 ± 0.031
5.246AlaIle: 5.246 ± 0.06
4.445AlaLys: 4.445 ± 0.065
7.287AlaLeu: 7.287 ± 0.071
1.887AlaMet: 1.887 ± 0.039
3.656AlaAsn: 3.656 ± 0.05
2.313AlaPro: 2.313 ± 0.038
2.92AlaGln: 2.92 ± 0.046
2.761AlaArg: 2.761 ± 0.039
4.796AlaSer: 4.796 ± 0.06
4.365AlaThr: 4.365 ± 0.086
5.08AlaVal: 5.08 ± 0.061
0.796AlaTrp: 0.796 ± 0.019
3.021AlaTyr: 3.021 ± 0.043
0.0AlaXaa: 0.0 ± 0.0
Cys
0.525CysAla: 0.525 ± 0.019
0.121CysCys: 0.121 ± 0.009
0.349CysAsp: 0.349 ± 0.014
0.331CysGlu: 0.331 ± 0.016
0.411CysPhe: 0.411 ± 0.02
0.576CysGly: 0.576 ± 0.02
0.173CysHis: 0.173 ± 0.011
0.555CysIle: 0.555 ± 0.018
0.461CysLys: 0.461 ± 0.017
0.709CysLeu: 0.709 ± 0.019
0.18CysMet: 0.18 ± 0.01
0.319CysAsn: 0.319 ± 0.015
0.278CysPro: 0.278 ± 0.014
0.224CysGln: 0.224 ± 0.013
0.313CysArg: 0.313 ± 0.014
0.491CysSer: 0.491 ± 0.018
0.382CysThr: 0.382 ± 0.015
0.407CysVal: 0.407 ± 0.017
0.087CysTrp: 0.087 ± 0.007
0.275CysTyr: 0.275 ± 0.015
0.0CysXaa: 0.0 ± 0.0
Asp
4.125AspAla: 4.125 ± 0.06
0.357AspCys: 0.357 ± 0.019
2.678AspAsp: 2.678 ± 0.049
3.395AspGlu: 3.395 ± 0.053
3.149AspPhe: 3.149 ± 0.05
3.933AspGly: 3.933 ± 0.055
1.201AspHis: 1.201 ± 0.032
3.726AspIle: 3.726 ± 0.05
3.44AspLys: 3.44 ± 0.047
5.326AspLeu: 5.326 ± 0.052
1.192AspMet: 1.192 ± 0.025
2.499AspAsn: 2.499 ± 0.042
2.172AspPro: 2.172 ± 0.041
2.444AspGln: 2.444 ± 0.041
2.35AspArg: 2.35 ± 0.037
2.628AspSer: 2.628 ± 0.042
2.515AspThr: 2.515 ± 0.043
3.651AspVal: 3.651 ± 0.048
0.739AspTrp: 0.739 ± 0.024
2.524AspTyr: 2.524 ± 0.039
0.0AspXaa: 0.0 ± 0.0
Glu
4.27GluAla: 4.27 ± 0.061
0.293GluCys: 0.293 ± 0.015
2.88GluAsp: 2.88 ± 0.045
3.932GluGlu: 3.932 ± 0.076
2.327GluPhe: 2.327 ± 0.039
3.643GluGly: 3.643 ± 0.052
1.252GluHis: 1.252 ± 0.029
4.174GluIle: 4.174 ± 0.056
4.401GluLys: 4.401 ± 0.062
6.057GluLeu: 6.057 ± 0.076
1.499GluMet: 1.499 ± 0.032
3.14GluAsn: 3.14 ± 0.042
1.567GluPro: 1.567 ± 0.029
2.776GluGln: 2.776 ± 0.04
2.798GluArg: 2.798 ± 0.04
2.949GluSer: 2.949 ± 0.042
2.89GluThr: 2.89 ± 0.041
4.043GluVal: 4.043 ± 0.05
0.634GluTrp: 0.634 ± 0.019
1.977GluTyr: 1.977 ± 0.033
0.0GluXaa: 0.0 ± 0.0
Phe
3.288PheAla: 3.288 ± 0.041
0.409PheCys: 0.409 ± 0.014
2.888PheAsp: 2.888 ± 0.042
2.818PheGlu: 2.818 ± 0.042
2.413PhePhe: 2.413 ± 0.039
3.401PheGly: 3.401 ± 0.052
0.833PheHis: 0.833 ± 0.022
3.353PheIle: 3.353 ± 0.051
3.115PheLys: 3.115 ± 0.048
4.309PheLeu: 4.309 ± 0.06
1.157PheMet: 1.157 ± 0.025
2.926PheAsn: 2.926 ± 0.041
1.77PhePro: 1.77 ± 0.03
1.451PheGln: 1.451 ± 0.03
2.071PheArg: 2.071 ± 0.034
4.096PheSer: 4.096 ± 0.052
2.872PheThr: 2.872 ± 0.053
2.795PheVal: 2.795 ± 0.047
0.59PheTrp: 0.59 ± 0.017
2.087PheTyr: 2.087 ± 0.035
0.0PheXaa: 0.0 ± 0.0
Gly
5.033GlyAla: 5.033 ± 0.088
0.57GlyCys: 0.57 ± 0.023
3.414GlyAsp: 3.414 ± 0.051
3.26GlyGlu: 3.26 ± 0.045
3.641GlyPhe: 3.641 ± 0.049
4.99GlyGly: 4.99 ± 0.097
1.224GlyHis: 1.224 ± 0.03
5.244GlyIle: 5.244 ± 0.058
5.267GlyLys: 5.267 ± 0.064
6.653GlyLeu: 6.653 ± 0.065
1.853GlyMet: 1.853 ± 0.031
3.839GlyAsn: 3.839 ± 0.062
1.629GlyPro: 1.629 ± 0.033
2.461GlyGln: 2.461 ± 0.04
2.814GlyArg: 2.814 ± 0.043
4.688GlySer: 4.688 ± 0.062
4.433GlyThr: 4.433 ± 0.087
4.369GlyVal: 4.369 ± 0.061
0.923GlyTrp: 0.923 ± 0.023
3.178GlyTyr: 3.178 ± 0.051
0.0GlyXaa: 0.0 ± 0.0
His
1.225HisAla: 1.225 ± 0.032
0.17HisCys: 0.17 ± 0.01
0.865HisAsp: 0.865 ± 0.022
1.047HisGlu: 1.047 ± 0.024
1.175HisPhe: 1.175 ± 0.027
1.121HisGly: 1.121 ± 0.029
0.582HisHis: 0.582 ± 0.019
1.324HisIle: 1.324 ± 0.031
0.999HisLys: 0.999 ± 0.028
1.928HisLeu: 1.928 ± 0.037
0.369HisMet: 0.369 ± 0.013
0.865HisAsn: 0.865 ± 0.023
1.006HisPro: 1.006 ± 0.025
0.941HisGln: 0.941 ± 0.024
0.777HisArg: 0.777 ± 0.022
1.015HisSer: 1.015 ± 0.025
1.019HisThr: 1.019 ± 0.023
0.97HisVal: 0.97 ± 0.028
0.23HisTrp: 0.23 ± 0.012
0.837HisTyr: 0.837 ± 0.022
0.0HisXaa: 0.0 ± 0.0
Ile
5.789IleAla: 5.789 ± 0.062
0.681IleCys: 0.681 ± 0.026
4.099IleAsp: 4.099 ± 0.05
3.908IleGlu: 3.908 ± 0.054
3.125IlePhe: 3.125 ± 0.051
4.742IleGly: 4.742 ± 0.06
1.268IleHis: 1.268 ± 0.026
4.51IleIle: 4.51 ± 0.06
4.393IleLys: 4.393 ± 0.06
6.016IleLeu: 6.016 ± 0.066
1.388IleMet: 1.388 ± 0.029
3.985IleAsn: 3.985 ± 0.046
2.972IlePro: 2.972 ± 0.051
2.324IleGln: 2.324 ± 0.04
3.193IleArg: 3.193 ± 0.048
5.489IleSer: 5.489 ± 0.061
4.207IleThr: 4.207 ± 0.057
3.979IleVal: 3.979 ± 0.046
0.711IleTrp: 0.711 ± 0.021
2.614IleTyr: 2.614 ± 0.045
0.0IleXaa: 0.0 ± 0.0
Lys
4.978LysAla: 4.978 ± 0.065
0.288LysCys: 0.288 ± 0.014
4.076LysAsp: 4.076 ± 0.056
4.364LysGlu: 4.364 ± 0.059
2.457LysPhe: 2.457 ± 0.042
4.537LysGly: 4.537 ± 0.058
1.217LysHis: 1.217 ± 0.03
4.703LysIle: 4.703 ± 0.055
5.134LysLys: 5.134 ± 0.064
5.784LysLeu: 5.784 ± 0.056
1.843LysMet: 1.843 ± 0.035
3.854LysAsn: 3.854 ± 0.05
2.512LysPro: 2.512 ± 0.044
2.498LysGln: 2.498 ± 0.045
2.67LysArg: 2.67 ± 0.037
3.947LysSer: 3.947 ± 0.048
3.97LysThr: 3.97 ± 0.054
4.359LysVal: 4.359 ± 0.049
0.742LysTrp: 0.742 ± 0.02
2.689LysTyr: 2.689 ± 0.041
0.0LysXaa: 0.0 ± 0.0
Leu
6.701LeuAla: 6.701 ± 0.081
0.761LeuCys: 0.761 ± 0.023
4.823LeuAsp: 4.823 ± 0.051
5.175LeuGlu: 5.175 ± 0.069
4.652LeuPhe: 4.652 ± 0.065
5.9LeuGly: 5.9 ± 0.065
1.676LeuHis: 1.676 ± 0.036
6.521LeuIle: 6.521 ± 0.075
7.332LeuLys: 7.332 ± 0.079
9.628LeuLeu: 9.628 ± 0.106
2.441LeuMet: 2.441 ± 0.044
5.809LeuAsn: 5.809 ± 0.062
4.124LeuPro: 4.124 ± 0.049
3.751LeuGln: 3.751 ± 0.05
4.115LeuArg: 4.115 ± 0.055
7.757LeuSer: 7.757 ± 0.08
5.504LeuThr: 5.504 ± 0.072
5.329LeuVal: 5.329 ± 0.059
0.952LeuTrp: 0.952 ± 0.027
3.282LeuTyr: 3.282 ± 0.043
0.0LeuXaa: 0.0 ± 0.0
Met
1.901MetAla: 1.901 ± 0.035
0.139MetCys: 0.139 ± 0.008
1.322MetAsp: 1.322 ± 0.03
1.589MetGlu: 1.589 ± 0.035
0.859MetPhe: 0.859 ± 0.02
1.616MetGly: 1.616 ± 0.03
0.417MetHis: 0.417 ± 0.018
1.632MetIle: 1.632 ± 0.033
2.041MetLys: 2.041 ± 0.037
2.34MetLeu: 2.34 ± 0.041
0.74MetMet: 0.74 ± 0.025
1.405MetAsn: 1.405 ± 0.026
1.097MetPro: 1.097 ± 0.025
0.927MetGln: 0.927 ± 0.025
1.019MetArg: 1.019 ± 0.025
1.544MetSer: 1.544 ± 0.032
1.303MetThr: 1.303 ± 0.031
1.635MetVal: 1.635 ± 0.031
0.176MetTrp: 0.176 ± 0.01
0.7MetTyr: 0.7 ± 0.02
0.0MetXaa: 0.0 ± 0.0
Asn
4.184AsnAla: 4.184 ± 0.06
0.337AsnCys: 0.337 ± 0.015
2.798AsnAsp: 2.798 ± 0.044
2.915AsnGlu: 2.915 ± 0.047
2.607AsnPhe: 2.607 ± 0.048
4.116AsnGly: 4.116 ± 0.068
0.937AsnHis: 0.937 ± 0.022
3.865AsnIle: 3.865 ± 0.046
3.365AsnLys: 3.365 ± 0.048
4.871AsnLeu: 4.871 ± 0.051
1.295AsnMet: 1.295 ± 0.027
3.056AsnAsn: 3.056 ± 0.069
2.616AsnPro: 2.616 ± 0.043
1.985AsnGln: 1.985 ± 0.037
2.289AsnArg: 2.289 ± 0.047
3.28AsnSer: 3.28 ± 0.053
3.304AsnThr: 3.304 ± 0.055
3.374AsnVal: 3.374 ± 0.046
0.682AsnTrp: 0.682 ± 0.022
2.5AsnTyr: 2.5 ± 0.05
0.0AsnXaa: 0.0 ± 0.0
Pro
3.221ProAla: 3.221 ± 0.055
0.193ProCys: 0.193 ± 0.011
2.51ProAsp: 2.51 ± 0.037
3.003ProGlu: 3.003 ± 0.044
1.915ProPhe: 1.915 ± 0.036
2.919ProGly: 2.919 ± 0.049
0.599ProHis: 0.599 ± 0.022
2.201ProIle: 2.201 ± 0.039
2.061ProLys: 2.061 ± 0.039
3.506ProLeu: 3.506 ± 0.046
0.843ProMet: 0.843 ± 0.023
1.794ProAsn: 1.794 ± 0.034
0.949ProPro: 0.949 ± 0.025
1.351ProGln: 1.351 ± 0.029
1.185ProArg: 1.185 ± 0.027
2.346ProSer: 2.346 ± 0.036
1.886ProThr: 1.886 ± 0.041
3.085ProVal: 3.085 ± 0.046
0.384ProTrp: 0.384 ± 0.014
1.513ProTyr: 1.513 ± 0.028
0.0ProXaa: 0.0 ± 0.0
Gln
2.71GlnAla: 2.71 ± 0.042
0.183GlnCys: 0.183 ± 0.01
1.786GlnAsp: 1.786 ± 0.032
2.301GlnGlu: 2.301 ± 0.042
1.679GlnPhe: 1.679 ± 0.032
2.176GlnGly: 2.176 ± 0.032
0.881GlnHis: 0.881 ± 0.025
2.487GlnIle: 2.487 ± 0.042
2.464GlnLys: 2.464 ± 0.039
4.37GlnLeu: 4.37 ± 0.066
0.981GlnMet: 0.981 ± 0.024
1.874GlnAsn: 1.874 ± 0.041
1.536GlnPro: 1.536 ± 0.035
2.355GlnGln: 2.355 ± 0.043
1.741GlnArg: 1.741 ± 0.035
2.321GlnSer: 2.321 ± 0.039
2.04GlnThr: 2.04 ± 0.037
2.536GlnVal: 2.536 ± 0.041
0.423GlnTrp: 0.423 ± 0.017
1.553GlnTyr: 1.553 ± 0.032
0.0GlnXaa: 0.0 ± 0.0
Arg
2.713ArgAla: 2.713 ± 0.045
0.228ArgCys: 0.228 ± 0.011
2.051ArgAsp: 2.051 ± 0.037
2.371ArgGlu: 2.371 ± 0.038
2.305ArgPhe: 2.305 ± 0.044
2.305ArgGly: 2.305 ± 0.038
0.744ArgHis: 0.744 ± 0.019
3.236ArgIle: 3.236 ± 0.042
3.029ArgLys: 3.029 ± 0.045
4.223ArgLeu: 4.223 ± 0.054
1.268ArgMet: 1.268 ± 0.026
2.461ArgAsn: 2.461 ± 0.039
1.436ArgPro: 1.436 ± 0.03
1.684ArgGln: 1.684 ± 0.034
1.833ArgArg: 1.833 ± 0.036
2.638ArgSer: 2.638 ± 0.041
2.212ArgThr: 2.212 ± 0.039
2.498ArgVal: 2.498 ± 0.04
0.572ArgTrp: 0.572 ± 0.017
2.009ArgTyr: 2.009 ± 0.039
0.0ArgXaa: 0.0 ± 0.0
Ser
5.14SerAla: 5.14 ± 0.059
0.586SerCys: 0.586 ± 0.02
3.294SerAsp: 3.294 ± 0.052
3.112SerGlu: 3.112 ± 0.048
3.668SerPhe: 3.668 ± 0.05
5.379SerGly: 5.379 ± 0.066
1.069SerHis: 1.069 ± 0.025
4.715SerIle: 4.715 ± 0.054
3.984SerLys: 3.984 ± 0.046
6.349SerLeu: 6.349 ± 0.066
1.53SerMet: 1.53 ± 0.033
3.426SerAsn: 3.426 ± 0.055
2.473SerPro: 2.473 ± 0.045
1.988SerGln: 1.988 ± 0.033
2.911SerArg: 2.911 ± 0.039
4.73SerSer: 4.73 ± 0.06
3.761SerThr: 3.761 ± 0.064
4.278SerVal: 4.278 ± 0.052
0.814SerTrp: 0.814 ± 0.018
3.003SerTyr: 3.003 ± 0.048
0.0SerXaa: 0.0 ± 0.0
Thr
5.017ThrAla: 5.017 ± 0.082
0.317ThrCys: 0.317 ± 0.013
3.323ThrAsp: 3.323 ± 0.048
3.111ThrGlu: 3.111 ± 0.038
2.799ThrPhe: 2.799 ± 0.051
4.84ThrGly: 4.84 ± 0.073
0.908ThrHis: 0.908 ± 0.025
4.087ThrIle: 4.087 ± 0.056
3.09ThrLys: 3.09 ± 0.042
5.551ThrLeu: 5.551 ± 0.069
1.125ThrMet: 1.125 ± 0.027
2.827ThrAsn: 2.827 ± 0.059
2.43ThrPro: 2.43 ± 0.047
1.785ThrGln: 1.785 ± 0.029
2.024ThrArg: 2.024 ± 0.038
3.562ThrSer: 3.562 ± 0.057
3.524ThrThr: 3.524 ± 0.077
3.966ThrVal: 3.966 ± 0.085
0.611ThrTrp: 0.611 ± 0.023
2.401ThrTyr: 2.401 ± 0.045
0.0ThrXaa: 0.0 ± 0.0
Val
4.859ValAla: 4.859 ± 0.054
0.494ValCys: 0.494 ± 0.02
3.339ValAsp: 3.339 ± 0.043
3.37ValGlu: 3.37 ± 0.051
3.095ValPhe: 3.095 ± 0.046
3.732ValGly: 3.732 ± 0.05
1.069ValHis: 1.069 ± 0.026
4.673ValIle: 4.673 ± 0.055
4.297ValLys: 4.297 ± 0.047
6.202ValLeu: 6.202 ± 0.073
1.664ValMet: 1.664 ± 0.033
3.584ValAsn: 3.584 ± 0.055
2.543ValPro: 2.543 ± 0.04
2.102ValGln: 2.102 ± 0.037
2.529ValArg: 2.529 ± 0.042
4.644ValSer: 4.644 ± 0.061
3.934ValThr: 3.934 ± 0.07
4.36ValVal: 4.36 ± 0.058
0.644ValTrp: 0.644 ± 0.021
2.467ValTyr: 2.467 ± 0.039
0.0ValXaa: 0.0 ± 0.0
Trp
0.741TrpAla: 0.741 ± 0.024
0.113TrpCys: 0.113 ± 0.009
0.616TrpAsp: 0.616 ± 0.02
0.652TrpGlu: 0.652 ± 0.017
0.542TrpPhe: 0.542 ± 0.016
0.78TrpGly: 0.78 ± 0.02
0.22TrpHis: 0.22 ± 0.011
0.747TrpIle: 0.747 ± 0.022
0.832TrpLys: 0.832 ± 0.021
1.137TrpLeu: 1.137 ± 0.026
0.366TrpMet: 0.366 ± 0.017
0.684TrpAsn: 0.684 ± 0.02
0.358TrpPro: 0.358 ± 0.014
0.482TrpGln: 0.482 ± 0.019
0.464TrpArg: 0.464 ± 0.018
0.693TrpSer: 0.693 ± 0.021
0.656TrpThr: 0.656 ± 0.02
0.659TrpVal: 0.659 ± 0.021
0.169TrpTrp: 0.169 ± 0.01
0.467TrpTyr: 0.467 ± 0.022
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.901TyrAla: 2.901 ± 0.037
0.315TyrCys: 0.315 ± 0.012
2.264TyrAsp: 2.264 ± 0.041
2.074TyrGlu: 2.074 ± 0.038
2.278TyrPhe: 2.278 ± 0.034
2.889TyrGly: 2.889 ± 0.054
0.942TyrHis: 0.942 ± 0.024
2.393TyrIle: 2.393 ± 0.039
2.474TyrLys: 2.474 ± 0.037
3.983TyrLeu: 3.983 ± 0.055
0.779TyrMet: 0.779 ± 0.023
2.371TyrAsn: 2.371 ± 0.047
1.684TyrPro: 1.684 ± 0.033
1.95TyrGln: 1.95 ± 0.035
1.959TyrArg: 1.959 ± 0.036
2.593TyrSer: 2.593 ± 0.042
2.521TyrThr: 2.521 ± 0.047
2.227TyrVal: 2.227 ± 0.041
0.501TyrTrp: 0.501 ± 0.018
1.845TyrTyr: 1.845 ± 0.038
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 5097 proteins (1742446 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski