Mycobacterium virus Fionnbarth
Average proteome isoelectric point is 6.33
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 94 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|G8IQZ5|G8IQZ5_9CAUD Uncharacterized protein OS=Mycobacterium virus Fionnbarth OX=1097753 GN=5 PE=4 SV=1
MM1 pKa = 7.0 ATLVRR6 pKa = 11.84 FIDD9 pKa = 3.85 PAAPEE14 pKa = 3.86 TEE16 pKa = 3.87 YY17 pKa = 11.39 RR18 pKa = 11.84 LDD20 pKa = 3.4 VGNFDD25 pKa = 3.59 VTTEE29 pKa = 4.09 EE30 pKa = 4.33 GLQALRR36 pKa = 11.84 DD37 pKa = 3.88 AMAEE41 pKa = 4.1 LCSDD45 pKa = 4.18 PSVVAVEE52 pKa = 4.21 EE53 pKa = 4.46 GMNGNLWMGEE63 pKa = 4.01 SLYY66 pKa = 10.79 FYY68 pKa = 11.04 AKK70 pKa = 9.3 LTTYY74 pKa = 7.57 PTLQDD79 pKa = 3.71 YY80 pKa = 11.21 VDD82 pKa = 4.1 SLSGGSVV89 pKa = 2.84
Molecular weight: 9.75 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.761
IPC2_protein 3.795
IPC_protein 3.706
Toseland 3.516
ProMoST 3.872
Dawson 3.694
Bjellqvist 3.859
Wikipedia 3.617
Rodwell 3.541
Grimsley 3.439
Solomon 3.656
Lehninger 3.617
Nozaki 3.821
DTASelect 3.973
Thurlkill 3.579
EMBOSS 3.63
Sillero 3.821
Patrickios 0.693
IPC_peptide 3.656
IPC2_peptide 3.795
IPC2.peptide.svr19 3.751
Protein with the highest isoelectric point:
>tr|G8IR62|G8IR62_9CAUD Uncharacterized protein OS=Mycobacterium virus Fionnbarth OX=1097753 GN=73 PE=4 SV=1
MM1 pKa = 7.55 GHH3 pKa = 5.5 RR4 pKa = 11.84 QKK6 pKa = 11.44 GKK8 pKa = 7.64 GCKK11 pKa = 9.47 RR12 pKa = 11.84 KK13 pKa = 10.06 RR14 pKa = 11.84 SWHH17 pKa = 5.81 RR18 pKa = 11.84 LGNQDD23 pKa = 2.84 RR24 pKa = 11.84 LYY26 pKa = 10.86 RR27 pKa = 11.84 RR28 pKa = 11.84 AIEE31 pKa = 4.55 ALDD34 pKa = 3.89 AAQQRR39 pKa = 11.84 AIEE42 pKa = 4.14 YY43 pKa = 10.81 AEE45 pKa = 3.88 VDD47 pKa = 3.19 DD48 pKa = 5.05 RR49 pKa = 11.84 EE50 pKa = 4.43 VVRR53 pKa = 11.84 RR54 pKa = 11.84 GAA56 pKa = 3.36
Molecular weight: 6.61 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.345
IPC2_protein 9.619
IPC_protein 10.526
Toseland 10.716
ProMoST 10.467
Dawson 10.804
Bjellqvist 10.54
Wikipedia 11.038
Rodwell 10.935
Grimsley 10.847
Solomon 10.965
Lehninger 10.921
Nozaki 10.701
DTASelect 10.54
Thurlkill 10.716
EMBOSS 11.125
Sillero 10.745
Patrickios 10.716
IPC_peptide 10.965
IPC2_peptide 9.589
IPC2.peptide.svr19 8.627
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
94
0
94
18532
27
1391
197.1
21.43
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
12.746 ± 0.513
1.085 ± 0.158
6.626 ± 0.261
5.93 ± 0.29
2.72 ± 0.176
8.677 ± 0.441
2.072 ± 0.177
3.766 ± 0.203
3.254 ± 0.2
8.364 ± 0.22
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.899 ± 0.099
2.93 ± 0.18
5.779 ± 0.24
3.497 ± 0.178
7.7 ± 0.409
4.954 ± 0.229
5.973 ± 0.208
7.646 ± 0.267
1.872 ± 0.097
2.509 ± 0.146
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here