Rotavirus C (isolate RVC/Human/United Kingdom/Bristol/1989) (RV-C)
Average proteome isoelectric point is 6.41
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 11 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>sp|Q91E95|RDRP_ROTHC RNA-directed RNA polymerase OS=Rotavirus C (isolate RVC/Human/United Kingdom/Bristol/1989) OX=31567 PE=3 SV=1
MM1 pKa = 7.33 VCTTLYY7 pKa = 8.78 TVCAILFILFIYY19 pKa = 10.33 ILLFRR24 pKa = 11.84 KK25 pKa = 8.46 MFHH28 pKa = 7.25 LITDD32 pKa = 4.02 TLIVILILSNCVEE45 pKa = 4.35 WSQGQMFTDD54 pKa = 4.69 DD55 pKa = 3.54 IYY57 pKa = 11.55 YY58 pKa = 10.17 NGNVEE63 pKa = 4.41 TIINSTDD70 pKa = 3.34 PFNVEE75 pKa = 3.85 SLCIYY80 pKa = 9.73 FPNAVVGSQGPGKK93 pKa = 10.4 SDD95 pKa = 2.77 GHH97 pKa = 8.08 LNDD100 pKa = 3.7 GNYY103 pKa = 10.46 AQTIATLFEE112 pKa = 4.43 TKK114 pKa = 10.21 GFPKK118 pKa = 10.58 GSIILKK124 pKa = 9.46 TYY126 pKa = 7.58 TQTSDD131 pKa = 4.08 FINSVEE137 pKa = 4.26 MTCSYY142 pKa = 10.97 NIVIIPDD149 pKa = 3.7 SPNDD153 pKa = 3.66 SEE155 pKa = 5.39 SIEE158 pKa = 4.82 QIAEE162 pKa = 3.94 WILNVWRR169 pKa = 11.84 CDD171 pKa = 3.57 DD172 pKa = 4.06 MNLEE176 pKa = 4.3 IYY178 pKa = 9.64 TYY180 pKa = 9.6 EE181 pKa = 3.99 QIGINNLWAAFGSDD195 pKa = 4.29 CDD197 pKa = 4.41 ISVCPLDD204 pKa = 3.68 TTSNGIGCSPASTEE218 pKa = 4.12 TYY220 pKa = 10.02 EE221 pKa = 4.47 VVSNDD226 pKa = 3.22 TQLALINVVDD236 pKa = 4.1 NVRR239 pKa = 11.84 HH240 pKa = 6.38 RR241 pKa = 11.84 IQMNTAQCKK250 pKa = 9.81 LKK252 pKa = 10.97 NCIKK256 pKa = 10.93 GEE258 pKa = 3.91 ARR260 pKa = 11.84 LNTALIRR267 pKa = 11.84 ISTSSSFDD275 pKa = 3.34 NSLSPLNNGQTTRR288 pKa = 11.84 SFKK291 pKa = 10.82 INAKK295 pKa = 9.15 KK296 pKa = 9.06 WWTIFYY302 pKa = 9.29 TIIDD306 pKa = 4.45 YY307 pKa = 11.08 INTIVQAMTPRR318 pKa = 11.84 HH319 pKa = 5.18 RR320 pKa = 11.84 AIYY323 pKa = 9.33 PEE325 pKa = 3.79 GWMLRR330 pKa = 11.84 YY331 pKa = 9.45 AA332 pKa = 4.23
Molecular weight: 37.47 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.624
IPC2_protein 4.749
IPC_protein 4.673
Toseland 4.52
ProMoST 4.8
Dawson 4.647
Bjellqvist 4.787
Wikipedia 4.533
Rodwell 4.52
Grimsley 4.431
Solomon 4.635
Lehninger 4.596
Nozaki 4.749
DTASelect 4.94
Thurlkill 4.533
EMBOSS 4.546
Sillero 4.8
Patrickios 0.807
IPC_peptide 4.647
IPC2_peptide 4.8
IPC2.peptide.svr19 4.7
Protein with the highest isoelectric point:
>sp|Q91E96|VP2_ROTHC Inner capsid protein VP2 OS=Rotavirus C (isolate RVC/Human/United Kingdom/Bristol/1989) OX=31567 PE=3 SV=1
MM1 pKa = 7.11 AQSIVVDD8 pKa = 3.94 GDD10 pKa = 3.44 YY11 pKa = 11.3 DD12 pKa = 3.97 ALASRR17 pKa = 11.84 YY18 pKa = 10.17 LKK20 pKa = 10.37 FVYY23 pKa = 10.25 DD24 pKa = 4.32 FEE26 pKa = 4.73 NVTYY30 pKa = 10.35 QNNYY34 pKa = 9.06 FATDD38 pKa = 3.29 KK39 pKa = 10.54 FKK41 pKa = 11.23 KK42 pKa = 10.42 DD43 pKa = 2.7 IEE45 pKa = 4.37 QYY47 pKa = 10.67 LKK49 pKa = 10.71 SIHH52 pKa = 7.03 DD53 pKa = 3.97 GEE55 pKa = 5.93 KK56 pKa = 8.92 ITQSKK61 pKa = 9.92 IDD63 pKa = 3.87 EE64 pKa = 4.38 KK65 pKa = 11.34 EE66 pKa = 3.93 KK67 pKa = 10.74 ILLDD71 pKa = 3.42 RR72 pKa = 11.84 VPAEE76 pKa = 4.13 EE77 pKa = 4.14 RR78 pKa = 11.84 CLISKK83 pKa = 9.67 LVFAYY88 pKa = 9.75 GKK90 pKa = 9.95 HH91 pKa = 5.52 GNVEE95 pKa = 4.13 NKK97 pKa = 9.43 LVKK100 pKa = 10.85 YY101 pKa = 9.66 GVKK104 pKa = 10.21 DD105 pKa = 3.86 ALSHH109 pKa = 7.09 APQKK113 pKa = 10.15 DD114 pKa = 3.2 AKK116 pKa = 9.9 PYY118 pKa = 8.58 EE119 pKa = 4.17 NNIITSEE126 pKa = 3.85 IFKK129 pKa = 10.54 EE130 pKa = 3.99 KK131 pKa = 10.8 SEE133 pKa = 4.07 YY134 pKa = 9.44 TDD136 pKa = 3.28 IYY138 pKa = 9.73 MDD140 pKa = 4.0 PSINTSCQSNCQAMMFTISEE160 pKa = 4.2 MKK162 pKa = 10.56 LNNIKK167 pKa = 10.44 NAARR171 pKa = 11.84 LEE173 pKa = 4.0 KK174 pKa = 10.67 LFTIIAATINKK185 pKa = 9.71 YY186 pKa = 10.66 GMPRR190 pKa = 11.84 HH191 pKa = 4.69 NTRR194 pKa = 11.84 YY195 pKa = 8.54 RR196 pKa = 11.84 YY197 pKa = 8.92 EE198 pKa = 3.9 WEE200 pKa = 3.94 TMKK203 pKa = 10.97 NKK205 pKa = 9.83 PYY207 pKa = 10.77 HH208 pKa = 5.31 LAAWINSSIEE218 pKa = 3.9 MIACVVDD225 pKa = 2.54 HH226 pKa = 6.41 HH227 pKa = 6.96 TYY229 pKa = 9.82 MIARR233 pKa = 11.84 EE234 pKa = 4.43 LIVKK238 pKa = 9.94 SFTNRR243 pKa = 11.84 TSLAKK248 pKa = 10.32 LVSSPMTVLTAMLPIRR264 pKa = 11.84 GTFITTEE271 pKa = 3.96 NLEE274 pKa = 4.19 LEE276 pKa = 4.45 YY277 pKa = 11.13 SNKK280 pKa = 10.12 SINYY284 pKa = 8.98 LISKK288 pKa = 9.53 EE289 pKa = 3.97 MAEE292 pKa = 5.71 DD293 pKa = 4.13 FMQAIKK299 pKa = 9.93 QLRR302 pKa = 11.84 DD303 pKa = 3.07 EE304 pKa = 4.36 GLEE307 pKa = 4.12 YY308 pKa = 10.56 IPDD311 pKa = 3.87 YY312 pKa = 9.78 YY313 pKa = 10.88 EE314 pKa = 4.07 KK315 pKa = 10.09 WFKK318 pKa = 11.34 SPDD321 pKa = 3.54 PLTFPNIALIYY332 pKa = 10.09 SFSFHH337 pKa = 6.17 VGYY340 pKa = 10.48 RR341 pKa = 11.84 KK342 pKa = 9.09 QALSDD347 pKa = 3.44 AVYY350 pKa = 10.62 DD351 pKa = 4.08 QITVTYY357 pKa = 9.29 SDD359 pKa = 3.67 NVNMEE364 pKa = 3.9 MYY366 pKa = 10.18 KK367 pKa = 10.24 EE368 pKa = 3.87 YY369 pKa = 10.86 SEE371 pKa = 5.63 RR372 pKa = 11.84 IEE374 pKa = 4.64 NEE376 pKa = 3.1 IFTILKK382 pKa = 10.31 DD383 pKa = 4.28 KK384 pKa = 10.58 IIHH387 pKa = 5.46 EE388 pKa = 4.44 DD389 pKa = 3.24 KK390 pKa = 10.86 RR391 pKa = 11.84 LEE393 pKa = 4.08 EE394 pKa = 4.38 YY395 pKa = 10.32 EE396 pKa = 4.46 LSALLSMSSASNGILRR412 pKa = 11.84 EE413 pKa = 4.16 INFGGQKK420 pKa = 9.72 VRR422 pKa = 11.84 STKK425 pKa = 10.96 KK426 pKa = 9.83 NMHH429 pKa = 6.66 VIDD432 pKa = 5.48 DD433 pKa = 4.54 IYY435 pKa = 10.49 HH436 pKa = 6.33 KK437 pKa = 10.54 KK438 pKa = 8.56 YY439 pKa = 7.24 TTDD442 pKa = 3.2 IPPVDD447 pKa = 3.97 ARR449 pKa = 11.84 NPIPLGRR456 pKa = 11.84 RR457 pKa = 11.84 DD458 pKa = 3.43 VPGRR462 pKa = 11.84 RR463 pKa = 11.84 TRR465 pKa = 11.84 AIFILPYY472 pKa = 9.96 QYY474 pKa = 10.79 FIAQHH479 pKa = 5.69 SFAEE483 pKa = 4.12 IMLNYY488 pKa = 10.12 AKK490 pKa = 10.44 RR491 pKa = 11.84 EE492 pKa = 3.84 RR493 pKa = 11.84 EE494 pKa = 3.69 YY495 pKa = 11.49 SEE497 pKa = 5.63 FYY499 pKa = 10.85 SQANQVLSYY508 pKa = 11.54 GDD510 pKa = 3.22 VTRR513 pKa = 11.84 YY514 pKa = 10.16 LDD516 pKa = 3.99 SNSILCFTDD525 pKa = 3.64 VSQWDD530 pKa = 3.74 ASQHH534 pKa = 4.09 NTKK537 pKa = 10.07 VLRR540 pKa = 11.84 RR541 pKa = 11.84 SIIRR545 pKa = 11.84 AMKK548 pKa = 10.01 RR549 pKa = 11.84 LKK551 pKa = 10.53 QLTHH555 pKa = 6.97 NINIHH560 pKa = 5.39 KK561 pKa = 10.19 AINIYY566 pKa = 9.94 IQSQEE571 pKa = 4.01 NLEE574 pKa = 4.01 NSYY577 pKa = 11.5 VLIDD581 pKa = 4.13 KK582 pKa = 10.65 KK583 pKa = 10.76 AIQYY587 pKa = 8.39 GATASGEE594 pKa = 4.15 KK595 pKa = 7.52 QTKK598 pKa = 9.23 IMNSIANKK606 pKa = 10.46 ALIQTVLGKK615 pKa = 11.04 LMTDD619 pKa = 3.18 YY620 pKa = 11.04 TFDD623 pKa = 3.17 VKK625 pKa = 10.38 MIRR628 pKa = 11.84 VDD630 pKa = 3.5 GDD632 pKa = 3.41 DD633 pKa = 3.45 NYY635 pKa = 11.59 AIVRR639 pKa = 11.84 FPIAITEE646 pKa = 4.16 KK647 pKa = 10.46 LLSEE651 pKa = 4.6 FTSKK655 pKa = 10.44 LRR657 pKa = 11.84 SYY659 pKa = 10.12 YY660 pKa = 10.58 SEE662 pKa = 3.99 MNVKK666 pKa = 9.88 VKK668 pKa = 10.67 ALASLTGCEE677 pKa = 3.38 IAKK680 pKa = 10.08 RR681 pKa = 11.84 YY682 pKa = 8.51 VAGGMLFFRR691 pKa = 11.84 AGVNILHH698 pKa = 6.39 HH699 pKa = 6.33 EE700 pKa = 4.14 KK701 pKa = 10.58 RR702 pKa = 11.84 NQDD705 pKa = 2.94 SAYY708 pKa = 11.06 DD709 pKa = 3.75 MAATLYY715 pKa = 11.24 ANYY718 pKa = 9.6 IVNALRR724 pKa = 11.84 GLTMSRR730 pKa = 11.84 TFILTKK736 pKa = 9.36 ICQMTSIKK744 pKa = 9.37 ITGTLRR750 pKa = 11.84 LFPMKK755 pKa = 10.44 SILALNSAFKK765 pKa = 11.19 VFDD768 pKa = 3.56 EE769 pKa = 4.01 VDD771 pKa = 3.45 YY772 pKa = 11.26 VINYY776 pKa = 7.54 PISNLFIQLQRR787 pKa = 11.84 KK788 pKa = 8.93 LSSIKK793 pKa = 10.48 AKK795 pKa = 10.72 SKK797 pKa = 10.22 IADD800 pKa = 4.2 NIAKK804 pKa = 10.1 SPQFKK809 pKa = 10.72 SYY811 pKa = 11.54 VEE813 pKa = 4.31 LLNKK817 pKa = 10.34 SLTTDD822 pKa = 3.35 EE823 pKa = 4.61 NPIVSDD829 pKa = 4.71 GIRR832 pKa = 11.84 LTEE835 pKa = 3.83 KK836 pKa = 10.91 AKK838 pKa = 10.75 LNSYY842 pKa = 10.86 APIALEE848 pKa = 3.65 KK849 pKa = 10.79 RR850 pKa = 11.84 RR851 pKa = 11.84 DD852 pKa = 3.54 QFSIMVSFLQNPTTFKK868 pKa = 11.02 SEE870 pKa = 4.21 TVVTINDD877 pKa = 3.21 VLYY880 pKa = 10.23 FISGFIKK887 pKa = 9.86 IDD889 pKa = 3.36 SSTVLPKK896 pKa = 10.75 EE897 pKa = 4.55 EE898 pKa = 4.46 NNTMPLLPAIIKK910 pKa = 8.17 RR911 pKa = 11.84 TLSYY915 pKa = 10.75 FGLRR919 pKa = 11.84 THH921 pKa = 7.59 DD922 pKa = 3.77 YY923 pKa = 10.17 DD924 pKa = 3.94 IKK926 pKa = 10.91 GSSSTVSKK934 pKa = 10.41 IIKK937 pKa = 9.37 QYY939 pKa = 10.99 SVYY942 pKa = 10.25 TPGIEE947 pKa = 3.87 EE948 pKa = 4.76 LYY950 pKa = 10.55 EE951 pKa = 4.01 IVNKK955 pKa = 10.66 SVDD958 pKa = 3.57 TIRR961 pKa = 11.84 GYY963 pKa = 10.03 FASFNVPKK971 pKa = 10.55 ADD973 pKa = 2.96 VDD975 pKa = 4.32 TYY977 pKa = 11.27 ISTQMYY983 pKa = 8.78 KK984 pKa = 10.03 HH985 pKa = 6.74 DD986 pKa = 4.07 RR987 pKa = 11.84 FKK989 pKa = 10.84 ILQAYY994 pKa = 8.83 IYY996 pKa = 10.59 NLLSVNYY1003 pKa = 10.43 GMYY1006 pKa = 10.44 QLVDD1010 pKa = 4.04 LNSARR1015 pKa = 11.84 FFDD1018 pKa = 4.73 HH1019 pKa = 7.35 VIHH1022 pKa = 6.49 TPMAKK1027 pKa = 9.17 TPTAVFMIDD1036 pKa = 2.87 LALRR1040 pKa = 11.84 LKK1042 pKa = 10.38 IINHH1046 pKa = 6.7 CIEE1049 pKa = 4.21 KK1050 pKa = 10.99 GEE1052 pKa = 4.39 IITVSVHH1059 pKa = 6.42 ANKK1062 pKa = 9.54 TDD1064 pKa = 3.68 YY1065 pKa = 11.32 LKK1067 pKa = 10.7 LWRR1070 pKa = 11.84 MLWNVKK1076 pKa = 7.82 TMNSPYY1082 pKa = 10.41 SKK1084 pKa = 11.05 NSMFDD1089 pKa = 3.17 EE1090 pKa = 4.64
Molecular weight: 125.8 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 8.333
IPC2_protein 8.39
IPC_protein 8.244
Toseland 8.785
ProMoST 8.741
Dawson 9.165
Bjellqvist 8.99
Wikipedia 9.355
Rodwell 9.311
Grimsley 9.238
Solomon 9.194
Lehninger 9.136
Nozaki 8.975
DTASelect 8.931
Thurlkill 9.004
EMBOSS 9.268
Sillero 9.151
Patrickios 4.266
IPC_peptide 9.18
IPC2_peptide 7.571
IPC2.peptide.svr19 7.628
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
11
0
11
5608
150
1090
509.8
58.59
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
5.314 ± 0.369
1.409 ± 0.457
5.617 ± 0.35
5.599 ± 0.514
4.297 ± 0.221
3.852 ± 0.416
1.819 ± 0.215
8.827 ± 0.326
6.758 ± 0.636
8.06 ± 0.383
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
3.156 ± 0.191
7.614 ± 0.446
2.889 ± 0.288
3.406 ± 0.372
4.494 ± 0.191
8.006 ± 0.571
6.758 ± 0.45
5.76 ± 0.287
1.088 ± 0.208
5.278 ± 0.485
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here