Flavobacteriaceae bacterium 144Ye

Taxonomy: cellular organisms; Bacteria; FCB group; Bacteroidetes/Chlorobi group; Bacteroidetes; Flavobacteriia; Flavobacteriales; Flavobacteriaceae; unclassified Flavobacteriaceae

Average proteome isoelectric point is 6.42

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2918 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A4V1VEB6|A0A4V1VEB6_9FLAO Uncharacterized protein OS=Flavobacteriaceae bacterium 144Ye OX=2510794 GN=EVU94_03760 PE=4 SV=1
MM1 pKa = 7.42YY2 pKa = 10.41LGQLTTYY9 pKa = 11.49NNMKK13 pKa = 10.0KK14 pKa = 10.5NIMKK18 pKa = 10.19VKK20 pKa = 9.88YY21 pKa = 8.68IWLLAVLLGFTACEE35 pKa = 4.0NDD37 pKa = 3.87DD38 pKa = 4.29DD39 pKa = 4.72SSNGGGMEE47 pKa = 4.71LPALTAGEE55 pKa = 4.48ADD57 pKa = 3.75FSNYY61 pKa = 8.93VSVGNSLTAGFSDD74 pKa = 3.31NALFIASQEE83 pKa = 3.92NSLPNILATQFAFAGGGSFTQPLMNDD109 pKa = 3.65NIGGLILGGTPVFHH123 pKa = 7.55PLTGEE128 pKa = 3.89QLFKK132 pKa = 10.77PRR134 pKa = 11.84LVTTGGAPQDD144 pKa = 4.01LEE146 pKa = 5.03DD147 pKa = 4.16VIGPVTSTTDD157 pKa = 3.58FLLNNPTGPFNNMGVPGAASFHH179 pKa = 6.72LIAPGYY185 pKa = 10.31GNIANFPAAANPYY198 pKa = 8.57FIRR201 pKa = 11.84MTGATPDD208 pKa = 3.4ASVLEE213 pKa = 4.32LAMAQSPTFFTLWAGNNDD231 pKa = 3.25VLGYY235 pKa = 8.57ATSGGDD241 pKa = 3.38GSSAITDD248 pKa = 3.23QATFDD253 pKa = 3.78FAINTLVTTLTSGGAKK269 pKa = 9.52GVMANIPDD277 pKa = 3.89VTSIPHH283 pKa = 5.86FTTVPHH289 pKa = 6.61NPLDD293 pKa = 3.8PTNEE297 pKa = 3.98DD298 pKa = 3.81FGPQIPLLNSIFGAINQIYY317 pKa = 8.97VALGQPEE324 pKa = 4.03RR325 pKa = 11.84AIVFSEE331 pKa = 4.58TEE333 pKa = 3.62ASAVVIKK340 pKa = 10.74DD341 pKa = 3.41EE342 pKa = 4.33SLDD345 pKa = 4.58DD346 pKa = 3.67ISAQITGALMASPTFPAFIAQFGLDD371 pKa = 3.62GSNPIVVQLVANLLGQTYY389 pKa = 9.56GQTRR393 pKa = 11.84QATEE397 pKa = 3.41NDD399 pKa = 3.89LFVLPSSSVIGEE411 pKa = 4.04VNMDD415 pKa = 3.51YY416 pKa = 11.44AGFLAGQGLPAEE428 pKa = 4.57LAGQFAIEE436 pKa = 5.6GITLPLEE443 pKa = 4.76DD444 pKa = 4.55KK445 pKa = 10.16WVLIPTEE452 pKa = 3.86QAEE455 pKa = 4.49IASATASFNATLEE468 pKa = 4.22TAATSAGLAFVDD480 pKa = 4.54ANSLMMQLASSGYY493 pKa = 9.35SDD495 pKa = 4.97GDD497 pKa = 3.58FTLTSDD503 pKa = 4.47LVTGGAFSLDD513 pKa = 3.36GVHH516 pKa = 5.99PTSRR520 pKa = 11.84GYY522 pKa = 11.36ALLANEE528 pKa = 4.15FMKK531 pKa = 11.1AIDD534 pKa = 3.51ATYY537 pKa = 10.49GSNFEE542 pKa = 4.27ASGNLVNIGNYY553 pKa = 5.75PTNYY557 pKa = 10.2SPTLQQ562 pKa = 3.54

Molecular weight: 58.71 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A4Q4B4U3|A0A4Q4B4U3_9FLAO O-succinylhomoserine sulfhydrylase OS=Flavobacteriaceae bacterium 144Ye OX=2510794 GN=metZ PE=3 SV=1
MM1 pKa = 7.69SKK3 pKa = 9.01RR4 pKa = 11.84TFQPSKK10 pKa = 9.13RR11 pKa = 11.84KK12 pKa = 9.48RR13 pKa = 11.84RR14 pKa = 11.84NKK16 pKa = 9.49HH17 pKa = 3.94GFRR20 pKa = 11.84EE21 pKa = 4.19RR22 pKa = 11.84MATANGRR29 pKa = 11.84KK30 pKa = 8.93VLARR34 pKa = 11.84RR35 pKa = 11.84RR36 pKa = 11.84AKK38 pKa = 10.09GRR40 pKa = 11.84KK41 pKa = 7.97KK42 pKa = 10.6LSVSSEE48 pKa = 4.04SRR50 pKa = 11.84HH51 pKa = 5.63KK52 pKa = 10.69KK53 pKa = 9.55

Molecular weight: 6.32 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2918

0

2918

1003851

38

3435

344.0

38.81

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.245 ± 0.04

0.777 ± 0.016

5.825 ± 0.036

6.573 ± 0.042

5.193 ± 0.036

6.135 ± 0.05

1.786 ± 0.023

7.826 ± 0.043

7.682 ± 0.074

9.156 ± 0.055

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.12 ± 0.024

6.496 ± 0.044

3.262 ± 0.026

3.411 ± 0.025

3.169 ± 0.034

6.601 ± 0.04

6.155 ± 0.064

6.411 ± 0.036

1.003 ± 0.017

4.176 ± 0.032
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski