Pycnoporus cinnabarinus (Cinnabar-red polypore) (Trametes cinnabarina)
Average proteome isoelectric point is 6.49
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 10180 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A060SKN8|A0A060SKN8_PYCCI Uncharacterized protein OS=Pycnoporus cinnabarinus OX=5643 GN=BN946_scf185010.g7 PE=4 SV=1
MM1 pKa = 7.65 RR2 pKa = 11.84 PASFFALLAMCITVQALPIAFTLADD27 pKa = 3.7 VSAGTLTSEE36 pKa = 4.59 GLFSTALSPVATSSPVAPTDD56 pKa = 3.5 SVSDD60 pKa = 3.96 VNSTAAIPTASTTDD74 pKa = 3.38 TSSDD78 pKa = 3.49 PASTDD83 pKa = 3.18 NANGASQTDD92 pKa = 3.73 PSIASATDD100 pKa = 3.73 DD101 pKa = 3.98 GSSDD105 pKa = 3.24 ASSTVVTDD113 pKa = 4.15 SSASPSSTATGDD125 pKa = 3.29 RR126 pKa = 11.84 ATGTDD131 pKa = 3.55 SPTSSAAQPTSTSVSFDD148 pKa = 3.26 IPVVPPACLALASGAQTTSLPTSTIASTAFTTLPPSGSSSLSNNTIISTIASNATATPSVTGSSAVSALPTDD220 pKa = 3.85 VASNGTLSRR229 pKa = 11.84 RR230 pKa = 11.84 IAQDD234 pKa = 3.32 DD235 pKa = 4.22 LPAVAQAWQDD245 pKa = 3.35 LCLVSGGDD253 pKa = 2.91 IFTNEE258 pKa = 4.2 PCVQLAGVNGINALLAHH275 pKa = 7.57 ADD277 pKa = 3.45 PCAQQDD283 pKa = 3.75 NADD286 pKa = 3.63 AMIDD290 pKa = 3.8 FAKK293 pKa = 10.81 SPGVTNSAALIANAVAYY310 pKa = 8.63 RR311 pKa = 11.84 QHH313 pKa = 6.58 PRR315 pKa = 11.84 NVLDD319 pKa = 3.4 ISGVLPSTPFCQRR332 pKa = 11.84 APRR335 pKa = 11.84 NAEE338 pKa = 3.53 LAGVVNGQLDD348 pKa = 3.89 GLNAGIFGSVGVGLFAFGADD368 pKa = 4.08 GSCPFGQVPDD378 pKa = 4.57 DD379 pKa = 4.0 TTCTCSS385 pKa = 2.97
Molecular weight: 38.05 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.722
IPC2_protein 3.617
IPC_protein 3.656
Toseland 3.414
ProMoST 3.872
Dawson 3.694
Bjellqvist 3.846
Wikipedia 3.681
Rodwell 3.478
Grimsley 3.325
Solomon 3.681
Lehninger 3.63
Nozaki 3.808
DTASelect 4.139
Thurlkill 3.49
EMBOSS 3.681
Sillero 3.795
Patrickios 1.888
IPC_peptide 3.668
IPC2_peptide 3.757
IPC2.peptide.svr19 3.743
Protein with the highest isoelectric point:
>tr|A0A060SJX4|A0A060SJX4_PYCCI Macro domain-containing protein OS=Pycnoporus cinnabarinus OX=5643 GN=BN946_scf184979.g41 PE=4 SV=1
LL1 pKa = 6.5 SQQMNPLMRR10 pKa = 11.84 APRR13 pKa = 11.84 SQLAARR19 pKa = 11.84 RR20 pKa = 11.84 FHH22 pKa = 5.19 QHH24 pKa = 6.2 HH25 pKa = 7.69 LIQTHH30 pKa = 6.26 LRR32 pKa = 11.84 PTPSPLPPHH41 pKa = 5.26 ATEE44 pKa = 4.59 RR45 pKa = 11.84 LLAGKK50 pKa = 9.87 GGRR53 pKa = 11.84 LHH55 pKa = 7.13 RR56 pKa = 11.84 PLSPKK61 pKa = 8.57 IWKK64 pKa = 8.37 WLPPRR69 pKa = 11.84 PMLRR73 pKa = 11.84 RR74 pKa = 11.84 LRR76 pKa = 11.84 HH77 pKa = 4.01 QTRR80 pKa = 11.84 GAPALHH86 pKa = 6.4 VVAAWALARR95 pKa = 11.84 QTGSRR100 pKa = 3.76
Molecular weight: 11.47 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.479
IPC2_protein 11.052
IPC_protein 12.647
Toseland 12.822
ProMoST 13.32
Dawson 12.822
Bjellqvist 12.808
Wikipedia 13.29
Rodwell 12.398
Grimsley 12.852
Solomon 13.32
Lehninger 13.217
Nozaki 12.822
DTASelect 12.808
Thurlkill 12.822
EMBOSS 13.32
Sillero 12.822
Patrickios 12.135
IPC_peptide 13.32
IPC2_peptide 12.31
IPC2.peptide.svr19 9.136
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
10180
0
10180
4930602
28
5011
484.3
53.33
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
9.526 ± 0.025
1.126 ± 0.009
5.72 ± 0.016
6.085 ± 0.026
3.534 ± 0.014
6.398 ± 0.02
2.623 ± 0.011
4.41 ± 0.019
4.333 ± 0.02
9.117 ± 0.024
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.982 ± 0.008
3.077 ± 0.012
6.986 ± 0.03
3.748 ± 0.016
6.589 ± 0.023
8.349 ± 0.033
5.816 ± 0.016
6.413 ± 0.017
1.397 ± 0.009
2.633 ± 0.012
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here